132 research outputs found

    Feasibility of unheated large gutter-connect greenhouses for winter organic vegetable production in Iowa

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    The researcher compared air and soil temperature dynamics, as well as growth and yield of crops in small hoop and large greenhouses, and evaluated the effects on temperature and crop performance of different row covers and row cover management

    Alien Registration- Mclaskey, Alice M. (Fryeburg, Oxford County)

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    https://digitalmaine.com/alien_docs/18078/thumbnail.jp

    Individual growth rate (IGR) and aminoacyl-tRNA synthetases (AARS) activity as individual-based indicators of growth rate of North Pacific krill, Euphausia pacifica

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    We investigated aminoacyl-tRNA synthetases (AARS) activity and individual growth rate (IGR) as individual-based in situ indicators of growth in adult krill, Euphausia pacifica. AARS enzymes catalyze the first step in protein synthesis while the IGR method is based on changes in body length during molting. Growth rates of field-collected krill were measured via the IGR method and individuals were subsequently preserved for AARS analysis to yield paired measurements. Our results show that conditions during the IGR incubation period influenced AARS activity in these individuals precluding a direct comparison but revealing the different timescales across which these two measures integrate. Importantly, they show that AARS activity provides a snap-shot image of an organism's metabolism, while IGR of krill is thought to integrate their environmental experience over several days. Each method would require repeated measurements to estimate population growth rates integrated over seasonal or generational time scales. As part of this project, we investigated how specific the AARS assay is to protein synthesis by testing a modified protocol that includes an additional blank and found evidence that the current assay may be measuring other cellular processes in addition to its intended signal. Our results suggest that a new NADH Blank might be optimized to improve the specificity of the assay.Postprint2,26

    Seismic moment tensor and b value variations over successive seismic cycles in laboratory stick-slip experiments

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    The formation of fault damage due to slip under high normal stresses can rarely be monitored under in situ conditions. To advance our understanding of microfracture processes, we investigated stick-slip events on Westerly granite samples containing the following: (1) a planar saw cut fault and (2) a fault developed from a fresh fracture surface. We examined temporal changes of seismic moment tensors and b values of acoustic emission (AE) events. During experiment on the saw cut surface, small AEs exhibiting non-double-couple components were observed continuously and strong AEs displaying double-couple components were visible only when approaching the slip onsets. Sliding on naturally fractured surfaces showed, in addition to double-couple components, significant volumetric contributions, especially during the interslip periods and immediately after stick-slip events indicating substantial shear-enhanced compaction within a relatively broad damage zone. The obtained results shed light on how differences in fault structure control the kinematics of microseismicity during different periods of the seismic cycle

    The high-frequency signature of slow and fast laboratory earthquakes

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    Tectonic faults fail through a spectrum of slip modes, ranging from slow aseismic creep to rapid slip during earthquakes. Understanding the seismic radiation emitted during these slip modes is key for advancing earthquake science and earthquake hazard assessment. In this work, we use laboratory friction experiments instrumented with ultrasonic sensors to document the seismic radiation properties of slow and fast laboratory earthquakes. Stick-slip experiments were conducted at a constant loading rate of 8 μm/s and the normal stress was systematically increased from 7 to 15 MPa. We produced a full spectrum of slip modes by modulating the loading stiffness in tandem with the fault zone normal stress. Acoustic emission data were recorded continuously at 5 MHz. We demonstrate that the full continuum of slip modes radiate measurable high-frequency energy between 100 and 500 kHz, including the slowest events that have peak fault slip rates <100 μm/s. The peak amplitude of the high-frequency time-domain signals scales systematically with fault slip velocity. Stable sliding experiments further support the connection between fault slip rate and high-frequency radiation. Experiments demonstrate that the origin of the high-frequency energy is fundamentally linked to changes in fault slip rate, shear strain, and breaking of contact junctions within the fault gouge. Our results suggest that having measurements close to the fault zone may be key for documenting seismic radiation properties and fully understanding the connection between different slip modes

    Characterization of rockfalls from seismic signal: insights from laboratory experiments

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    International audienceThe seismic signals generated by rockfalls can provide information on their dynamics and location. However, the lack of field observations makes it difficult to establish clear relationships between the characteristics of the signal and the source. In this study, scaling laws are derived from analytical impact models to relate the mass and the speed of an individual impactor to the radiated elastic energy and the frequency content of the emitted seismic signal. It appears that the radiated elastic energy and frequencies decrease when the impact is viscoelastic or elasto-plastic compared to the case of an elastic impact. The scaling laws are validated with laboratory experiments of impacts of beads and gravels on smooth thin plates and rough thick blocks. Regardless of the involved materials, the masses and speeds of the impactors are retrieved from seismic measurements within afactor of 3. A quantitative energy budget of the impacts is established. On smooth thin plates, the lost energy is either radiated in elastic waves or dissipated in viscoelasticity when the impactor is large or small with respect to the plate thickness, respectively. In contrast, on rough thick blocks, theelastic energy radiation represents less than 5% of the lost energy. Most of the energy is lost in plastic deformation or rotation modes of the bead owingto surface roughness. Finally, we estimate the elastic energy radiated during field scale rockfalls experiments. This energy is shown to be proportional to the boulder mass, in agreement with the theoretical scaling laws

    Eco-physiological responses of copepods and pteropods to ocean warming and acidification

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    We compare physiological responses of the crustacean copepod Calanus pacificus and pelagic pteropod mollusk Limacina helicina to ocean temperatures and pH by measuring biomarkers of oxidative stress, antioxidant defences, and the activity of the respiratory electron transport system in organisms collected on the 2016 West Coast Ocean Acidification cruise in the California Current System. Copepods and pteropods exhibited strong but divergent responses in the same habitat; copepods had higher oxygen-reactive absorbance capacity, glutathione-S-transferase, and total glutathione content. The ratio between reduced to oxidised glutathione was higher in copepods than in pteropods, indicating lower oxidative stress in copepods. Pteropods showed higher activities of glutathione reductase, catalase, and lipid peroxidation, indicating increased antioxidant defences and oxidative stress. Thus, the antioxidant defence system of the copepods has a greater capacity to respond to oxidative stress, while pteropods already face severe stress and show limited capacity to deal with further changes. The results suggest that copepods have higher adaptive potential, owing to their stronger vertical migration behaviour and efficient glutathione metabolism, whereas pteropods run the risk of oxidative stress and mortality under high CO2 conditions. Our results provide a unique dataset and evidence of stress-inducing mechanisms behind pteropod ocean acidification responses.</p

    Seismic energy analysis as generated by impact and fragmentation of single-block experimental rockfalls

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    The analysis of seismic signals obtained from near-source triaxial accelerometer recordings of two sets of single-block rockfall experiments is presented. The tests were carried out under controlled conditions in two quarries in northeastern Spain; in the first test (Foj limestone quarry, Barcelona), 30 blocks were released with masses ranging between 475 and 11,480 kg. The second test (Ponderosa andesite quarry, Tarragona) consisted of the release of 44 blocks with masses from 466 to 13,581 kg. An accelerometer and three high-speed video cameras were deployed, so that the trajectories, velocities, and block fragmentation could be tracked precisely. These data were used to explore the relationship between seismic energy and rockfall kinetics (the latter obtained from video analysis). We determined absolute and relative values of seismic energy and used them to estimate rockfall volumes. Finally, the seismic signature of block fragmentation was assessed in both the frequency and time domains. The ratios of seismic energy after impact to kinetic energy before impact ranged between 10-7 and 10-4. These variables were weakly correlated. The use of seismic energy relative to impacting kinetic energy was preferred for the estimation of volumes. Block fragmentation impacts were dominated by higher acceleration spectrum centroid frequencies than those of nonfragmentation impacts: 56.62 ± 2.88 and 48.46 ± 4.39 Hz at Foj and 52.84 ± 12.73 and 38.14 ± 4.73 Hz at Ponderosa.Peer ReviewedPostprint (published version

    Alien Registration- Mclaskey, Colin L. (Brownfield, Oxford County)

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    https://digitalmaine.com/alien_docs/13271/thumbnail.jp
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