20 research outputs found
The Ink4a/Arf locus operates as a regulator of the circadian clock modulating RAS activity
The mammalian circadian clock and the cell cycle are two major biological oscillators whose coupling influences cell fate decisions. In the present study, we use a model-driven experimental approach to investigate the interplay between clock and cell cycle components and the dysregulatory effects of RAS on this coupled system. In particular, we focus on the Ink4a/Arf locus as one of the bridging clock-cell cycle elements. Upon perturbations by the rat sarcoma viral oncogene (RAS), differential effects on the circadian phenotype were observed in wild-type and Ink4a/Arf knock-out mouse embryonic fibroblasts (MEFs), which could be reproduced by our modelling simulations and correlated with opposing cell cycle fate decisions. Interestingly, the observed changes can be attributed to in silico phase shifts in the expression of core-clock elements. A genome-wide analysis revealed a set of differentially expressed genes that form an intricate network with the circadian system with enriched pathways involved in opposing cell cycle phenotypes. In addition, a machine learning approach complemented by cell cycle analysis classified the observed cell cycle fate decisions as dependent on Ink4a/Arf and the oncogene RAS and highlighted a putative fine-tuning role of Bmal1 as an elicitor of such processes, ultimately resulting in increased cell proliferation in the Ink4a/Arf knock-out scenario. This indicates that the dysregulation of the core-clock might work as an enhancer of RAS-mediated regulation of the cell cycle. Our combined in silico and in vitro approach highlights the important role of the circadian clock as an Ink4a/Arf-dependent modulator of oncogene-induced cell fate decisions, reinforcing its function as a tumour-suppressor and the close interplay between the clock and the cell cycle network
Photography-based taxonomy is inadequate, unnecessary, and potentially harmful for biological sciences
The question whether taxonomic descriptions naming new animal species without type specimen(s) deposited in collections should be accepted for publication by scientific journals and allowed by the Code has already been discussed in Zootaxa (Dubois & NemĂ©sio 2007; Donegan 2008, 2009; NemĂ©sio 2009aâb; Dubois 2009; Gentile & Snell 2009; Minelli 2009; Cianferoni & Bartolozzi 2016; Amorim et al. 2016). This question was again raised in a letter supported
by 35 signatories published in the journal Nature (Pape et al. 2016) on 15 September 2016. On 25 September 2016, the following rebuttal (strictly limited to 300 words as per the editorial rules of Nature) was submitted to Nature, which on
18 October 2016 refused to publish it. As we think this problem is a very important one for zoological taxonomy, this text is published here exactly as submitted to Nature, followed by the list of the 493 taxonomists and collection-based
researchers who signed it in the short time span from 20 September to 6 October 2016
EXORDIUM regulates brassinosteroid-responsive genes
AbstractIn a screen for potential mediators of brassinosteroid (BR) effects, the EXORDIUM (EXO) protein was identified as a regulator of BR-responsive genes. The EXO gene was characterized as a BR-up-regulated gene. EXO overexpression under the control of the 35SCaMV promoter resulted in increased transcript levels of the BR-up-regulated KCS1, Exp5, ÎŽ-TIP, and AGP4 genes, which likely are involved in the mediation of BR-promoted growth. 35S::EXO lines grown in soil or in synthetic medium showed increased vegetative growth in comparison to wild-type plants, resembling the growth phenotype of BR-treated plants. Thus, the EXO protein most likely promotes growth via the modulation of gene expression patterns
Order reduction stiffness and mass matrices Part 2 - Lanczos method
Translated from Slovak (Report of the Inst. of Materials and Machine Mechanics, Slovak Academy of Sciences, Martin, 1996)SIGLEAvailable from British Library Document Supply Centre-DSC:9023.190(VR-Trans--8297)T / BLDSC - British Library Document Supply CentreGBUnited Kingdo