Bioconjugated, Immunological and Synthetic Nanoplatforms for diagnoses and development of new treatments for infectious diseases.

En esta comunicación se ha abordado diferentes temas implicados en el desarrollo de nuevos tratamientos y vacunas aplicadas a patologías desarrolladas en el ser humano tal como las producidas por virus. En este contexto se han presentado y discutido diferentes estrategias utilizadas, las cuales contemplan desde el diseño de moléculas pequeñas hacia mayores estructuras químicas y nuevas Nano-estructuras. En particular se ha discutido estudios para el tratamiento del Corona Virus; los cuales permitieron el planteo de los principales mecanismos de acción de fármacos y Nanoparticulas con funcionalidades específicas. En esta dirección se ha destacado la importancia de la Bioconjugación de moléculas y variables Nano-arquitecturas para la incorporación dentro de células y desarrollo de vacunas. Además, se ha tratado sobre el desarrollo de tratamientos combinados en base a diferentes estrategias aplicadas. En este mismo sentido, finalmente se han presentados diferentes estudios y desarrollos actualmente en progreso, relacionados con el diseño de Nanoarquitecturas funcionales o Multifuncionales, con potencial implicación en tratamientos avenir.In this communication it was addressed different themes and topics implicated in the development of new treatments and vaccines applied to pathologies developed in humans such as by Virus. In this context, it was presented and discussed different strategies used, which were contemplated from the design of small molecules, towards higher sized chemical structures and new Nanoarchitectures. In particular, it was discussed varied studies developed for the Corona Virus treatment; which afforded to the main mechanisms of action of pharmacophores and targeted functional Nanoparticles. In this direction, it was highlighted the importance of Bioconjugation of molecules and variable Nanoarchitectures for their incorporation within cells and for the development of vaccines. Moreover, it was discussed about the development of combinatory treatments based on different applied strategies. In similar manner, finally it was presented different studies and developments actually in progress related with the design of functional and Multifunctional Nanoarchitectures with potential implications in future treatments.Fil: Gomez Palacios, Luna R. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Martinez, Sofia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; ArgentinaFil: Tettamanti, Cecilia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; ArgentinaFil: Quinteros, Daniela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; ArgentinaFil: Bracamonte, Angel Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina. Laval University; Canad

Bioconjugated, Immunological and Synthetic Nanoplatforms for diagnoses and development of new treatments for infectious diseases.

En esta comunicación se ha abordado diferentes temas implicados en el desarrollo de nuevos tratamientos y vacunas aplicadas a patologías desarrolladas en el ser humano tal como las producidas por virus. En este contexto se han presentado y discutido diferentes estrategias utilizadas, las cuales contemplan desde el diseño de moléculas pequeñas hacia mayores estructuras químicas y nuevas Nano-estructuras. En particular se ha discutido estudios para el tratamiento del Corona Virus; los cuales permitieron el planteo de los principales mecanismos de acción de fármacos y Nanoparticulas con funcionalidades específicas. En esta dirección se ha destacado la importancia de la Bioconjugación de moléculas y variables Nano-arquitecturas para la incorporación dentro de células y desarrollo de vacunas. Además, se ha tratado sobre el desarrollo de tratamientos combinados en base a diferentes estrategias aplicadas. En este mismo sentido, finalmente se han presentados diferentes estudios y desarrollos actualmente en progreso, relacionados con el diseño de Nanoarquitecturas funcionales o Multifuncionales, con potencial implicación en tratamientos avenir.In this communication it was addressed different themes and topics implicated in the development of new treatments and vaccines applied to pathologies developed in humans such as by Virus. In this context, it was presented and discussed different strategies used, which were contemplated from the design of small molecules, towards higher sized chemical structures and new Nanoarchitectures. In particular, it was discussed varied studies developed for the Corona Virus treatment; which afforded to the main mechanisms of action of pharmacophores and targeted functional Nanoparticles. In this direction, it was highlighted the importance of Bioconjugation of molecules and variable Nanoarchitectures for their incorporation within cells and for the development of vaccines. Moreover, it was discussed about the development of combinatory treatments based on different applied strategies. In similar manner, finally it was presented different studies and developments actually in progress related with the design of functional and Multifunctional Nanoarchitectures with potential implications in future treatments.Fil: Gomez Palacios, Luna R. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Martinez, Sofia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; ArgentinaFil: Tettamanti, Cecilia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; ArgentinaFil: Quinteros, Daniela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; ArgentinaFil: Bracamonte, Angel Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina. Laval University; Canad

Clinical usefulness of infrared thermography to detect sick animals: frequent and current cases

Infrared thermography (IRT) is a tool that has been studied extensively in the experimental medical field as a method for assessing surface thermal responses under various conditions. These may involve local inflammatory processes resulting from surgical procedures, wounds, neoplasms, pathologies, painful events, or stressful states in animals. IRT measures changes in blood flow in surface blood capillaries and the resulting heat radiation. In the clinical field, thermography has been used as a support method for detecting painful conditions. However, some guidelines indicate that it could be applied for assessing and monitoring animals in rehabilitation to quantify objectively possible improvements in their quality of life. Similarly, IRT makes it possible to assess the degree of circulation in dermal tissue, suggesting that it could be used to determine the degree of damage in traumatized tissue in cases of thromboembolic diseases and burns. This would be useful to distinguish between damaged and healthy tissue and thus determine the optimal therapy for burn patients. This review aims to analyze scientific evidence on the clinical applications of IRT for detecting diseases and assessing painful conditions. A literature search on different databases was performed to recover articles related to the application of IRT as a complementary diagnostic tool, and its potential for assisting in rehabilitation, monitoring wounds, and evaluating body temperature in domestic animals

The curse of the uncultured fungus

The international DNA sequence databases abound in fungal sequences not annotated beyond the kingdom level, typically bearing names such as "uncultured fungus". These sequences beget lowresolution mycological results and invite further deposition of similarly poorly annotated entries. What do these sequences represent? This study uses a 767,918-sequence corpus of public full-length that represent truly unidentifiable fungal taxa - and what proportion of them that would have deposition. Our results suggest that more than 70% of these sequences would have been trivial to identify to at least the order/family level at the time of sequence deposition, hinting that factors other than poor availability of relevant reference sequences explain the low-resolution names. We speculate that researchers' perceived lack of time and lack of insight into the ramifications of this problem are the main explanations for the low-resolution names. We were surprised to find that more than a fifth of these sequences seem to have been deposited by mycologists rather than researchers unfamiliar with the consequences of poorly annotated fungal sequences in molecular repositories. The proportion of these needlessly poorly annotated sequences does not decline over time, suggesting that this problem must not be left unchecked

How, not if, is the question mycologists should be asking about DNA-based typification

Fungal metabarcoding of substrates such as soil, wood, and water is uncovering an unprecedented number of fungal species that do not seem to produce tangible morphological structures and that defy our best attempts at cultivation, thus falling outside the scope of the International Code of Nomenclature for algae, fungi, and plants. The present study uses the new, ninth release of the species hypotheses of the UNITE database to show that species discovery through environmental sequencing vastly outpaces traditional, Sanger sequencing-based efforts in a strongly increasing trend over the last five years. Our findings chal-lenge the present stance of some in the mycological community - that the current situation is satisfactory and that no change is needed to "the code" - and suggest that we should be discussing not whether to allow DNA-based descriptions (typifications) of species and by extension higher ranks of fungi, but what the precise requirements for such DNA-based typifications should be. We submit a tentative list of such criteria for further discussion. The present authors hope for a revitalized and deepened discussion on DNA-based typification, because to us it seems harmful and counter-productive to intentionally deny the overwhelming majority of extant fungi a formal standing under the International Code of Nomenclature for algae, fungi, and plants

Intervención de urbanismo táctico para la implementación de cruces seguros

A lo largo de este reporte se informa sobre las distintas labores, investigaciones y visitas de campo que se llevan a cabo en el Proyecto de Aplicación Profesional de Movilidad en el periodo de primavera 2021. Durante el cual se abordó la problemática de movilidad urbana y falta de infraestructura que cotidianamente experimentan los usuarios que atraviesan el cruce de Av. Ramón Corona, Colegiales, Libertad y C. Degollado, denominado “Área de intervención”, ubicado en la colonia Mexicaltzingo, Guadalajara, Jal. La confluencia de diferentes dinámicas de las unidades transporte público colectivo en la zona, así como la falta de regulación y organización de las mismas generan un evidente desorden en materia de movilidad en el cruce de las calles anteriormente mencionadas, dicha problemática sumada a la ausencia infraestructura y señalética urbana adecuada que dote de claridad al espacio, pone en riesgo la vida de miles de usuarios que hacen uso del mismo diariamente, especialmente al peatón ya que la complejidad de la intersección de varias vialidades sumado a las falta de claridad en el espacio y los movimientos de transporte motorizado generan cruces inseguros entre vialidades que exponen al peatón. El objetivo principal de este trabajo es generar una intervención de urbanismo táctico así como una propuesta formal de implementación de cruces seguros en la intersección anteriormente mencionada, esto con la finalidad de organizar los movimientos peatonales en vialidades del área de intervención. La propuesta desarrollada podrá servir como un incentivo para que las autoridades correspondientes intervengan y ejecuten un proyecto de urbanismo permanente en base a la intervención de urbanismo táctico y la propuesta formal generada como resultado de este proyecto. Utilizamos una metodología de investigación mixta, por un lado utilizamos un abordaje cuantitativo para observar elementos medibles en el espacio, mientras que el abordaje cualitativo fue útil para obtener información acerca de la calidad del mismo.ITESO, A.C

Seroprevalence and Risk Factors Possibly Associated with Emerging Zoonotic Vaccinia Virus in a Farming Community, Colombia

In 2014, vaccinia virus (VACV) infections were identified among farmworkers in Caquetá Department, Colombia; additional cases were identified in Cundinamarca Department in 2015. VACV, an orthopoxvirus (OPXV) used in the smallpox vaccine, has caused sporadic bovine and human outbreaks in countries such as Brazil and India. In response to the emergence of this disease in Colombia, we surveyed and collected blood from 134 farmworkers and household members from 56 farms in Cundinamarca Department. We tested serum samples for OPXV antibodies and correlated risk factors with seropositivity by using multivariate analyses. Fifty-two percent of farmworkers had OPXV antibodies; this percentage decreased to 31% when we excluded persons who would have been eligible for smallpox vaccination. The major risk factors for seropositivity were municipality, age, smallpox vaccination scar, duration of time working on a farm, and animals having vaccinia-like lesions. This investigation provides evidence for possible emergence of VACV as a zoonosis in South America.https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000318507https://scholar.google.com.co/citations?user=cU2KyT4AAAAJ&hl=enhttps://scienti.minciencias.gov.co/gruplac/jsp/visualiza/visualizagr.jsp?nro=00000000008981https://orcid.org/0000-0002-8093-054

Energy Estimation of Cosmic Rays with the Engineering Radio Array of the Pierre Auger Observatory

The Auger Engineering Radio Array (AERA) is part of the Pierre Auger Observatory and is used to detect the radio emission of cosmic-ray air showers. These observations are compared to the data of the surface detector stations of the Observatory, which provide well-calibrated information on the cosmic-ray energies and arrival directions. The response of the radio stations in the 30 to 80 MHz regime has been thoroughly calibrated to enable the reconstruction of the incoming electric field. For the latter, the energy deposit per area is determined from the radio pulses at each observer position and is interpolated using a two-dimensional function that takes into account signal asymmetries due to interference between the geomagnetic and charge-excess emission components. The spatial integral over the signal distribution gives a direct measurement of the energy transferred from the primary cosmic ray into radio emission in the AERA frequency range. We measure 15.8 MeV of radiation energy for a 1 EeV air shower arriving perpendicularly to the geomagnetic field. This radiation energy -- corrected for geometrical effects -- is used as a cosmic-ray energy estimator. Performing an absolute energy calibration against the surface-detector information, we observe that this radio-energy estimator scales quadratically with the cosmic-ray energy as expected for coherent emission. We find an energy resolution of the radio reconstruction of 22% for the data set and 17% for a high-quality subset containing only events with at least five radio stations with signal.Comment: Replaced with published version. Added journal reference and DO

Measurement of the Radiation Energy in the Radio Signal of Extensive Air Showers as a Universal Estimator of Cosmic-Ray Energy

We measure the energy emitted by extensive air showers in the form of radio emission in the frequency range from 30 to 80 MHz. Exploiting the accurate energy scale of the Pierre Auger Observatory, we obtain a radiation energy of 15.8 \pm 0.7 (stat) \pm 6.7 (sys) MeV for cosmic rays with an energy of 1 EeV arriving perpendicularly to a geomagnetic field of 0.24 G, scaling quadratically with the cosmic-ray energy. A comparison with predictions from state-of-the-art first-principle calculations shows agreement with our measurement. The radiation energy provides direct access to the calorimetric energy in the electromagnetic cascade of extensive air showers. Comparison with our result thus allows the direct calibration of any cosmic-ray radio detector against the well-established energy scale of the Pierre Auger Observatory.Comment: Replaced with published version. Added journal reference and DOI. Supplemental material in the ancillary file