HELECHOS Y LICOFITAS: ACTUALIZACIÓN DE LA FLORA DEL VALLE DE LERMA – SALTA, ARGENTINA

Se presenta un listado actualizado de la diversidad de helechos y licófitas del Valle de Lerma, Salta (Argentina), como resultado de la revisión bibliográfica, la consulta de los ejemplares depositados en herbarios nacionales y colectas hechas ex profeso. Se registran 115 especies, nueve correspondientes a actualizaciones taxonómicas y 16 a nuevos registros respecto a la publicación original, dos de ellos se citan por primera vez para la región noroeste de Argentina: Microgramma vaccinifolia y Pellaea atropurpurea. La gran mayoría son plantas nativas, cuatro crecen como naturalizadas: Adiantum peruvianum, Pellaea atropurpurea, Pteris cretica y P. vittata. De las 17 familias presentes, las mejor representadas son Pteridaceae con 33 especies, Polypodiaceae con 19 especies, Aspleniaceae con 15 especies y Dryopteridaceae con 11 especies. La diversidad de helechos y licófitas para el Valle de Lerma representa el 27 % de la citada para la flora argentina

Huperzia reflexa y Lycopodiella cernua (Lycopodiales-Pteridophyta): dos nuevas citas para el NO de la Argentina y actualizaciones sobre su morfología

Huperzia reflexa and Lycopodiella cernua are recorded for the first time for the northwest of Argentina; along with the new records, the characters of the leaf morphology and spores are analyzed, and updated descriptions and geographical distribution of both taxa are also given. Three of the four genera of the Lycopodiaceae are present so far in the area: Huperzia, Lycopodium and Lycopodiella, and the number of species come to nine. To facilitate the recognition of all taxa, this paper also includes a key for their determination.Huperzia reflexa y Lycopodiella cernua se citan por primera vez para el noroeste de la Argentina, se analizan aspectos característicos de su morfología foliar y esporal, se actualizan las descripciones de ambos taxones y su distribución geográfica. Con la presencia de Lycopodiella cernua, se encuentran representados en el área tres de los cuatro géneros de Lycopodiaceae, Huperzia, Lycopodiella y Lycopodium, con un total de nueve especies. Para facilitar el reconocimiento de todas las especies de Lycopodiaceae presentes hasta el momento en la región, en este trabajo se incluye también una clave para su determinación

Accessible lifelong learning at higher education:outcomes and lessons Learned at two different PilotSites in the EU4ALL Project

[EN] The EU4ALL project (IST-FP6-034778) has developed a general framework to address the needs of accessible lifelong learning at Higher Education level consisting of several standards-based interoperable components integrated into an open web service architecture aimed at supporting adapted interaction to guarantee students' accessibility needs. Its flexibility has supported the project implementation at several sites with different settings and various learning management systems. Large-scale evaluations involving hundreds of users, considering diverse disability types, and key staff roles have allowed obtaining valuable lessons with respect to "how to adopt or enhance eLearning accessibility" at university. The project was evaluated at four higher education institutions, two of the largest in Europe and two mediumsized. In this paper, we focus on describing the implementation and main conclusions at the largest project evaluation site (UNED), which was involved in the project from the beginning, and thus, in the design process, and a medium-sized university that adopted the EU4ALL approach (UPV). This implies dealing with two well-known open source learning environments (i.e. dotLRN and Sakai), and considering a wide variety of stakeholders and requirements. Thus the results of this evaluation serve to illustrate the coverage of both the approach and developments.The authors would like to thank the European Commission for the financial support of the EU4ALL project (IST-2006-034478). The work at aDeNu is also supported by the Spanish Ministry of Science and Innovation (TIN2008-06862-C04-01/TSI “A2UN@”). Authors would also like to thank all the EU4ALL partners for their collaboration.Boticario, JG.; Rodriguez-Ascaso, A.; Santos, OC.; Raffenne, E.; Montandon, L.; Roldán Martínez, D.; Buendía García, F. (2012). Accessible lifelong learning at higher education:outcomes and lessons Learned at two different PilotSites in the EU4ALL Project. Journal of Universal Computer Science. 18(1):62-85. http://hdl.handle.net/10251/37117628518

Glucocorticoid receptor intestinal epithelial knockout mice show attenuated colonic inflammatory response but unaffected permeability in early experimental sepsis

Introduction: Sepsis is defined as an organic dysfunction that threatens the life of patients due to an abnormally regulated response to infection [1]. The initial phase of sepsis is dominated by an increased production of proinflammatory cytokines, which leads to augmented capillary permeability, extravasation, hypercoagulability and myelopoiesis. One of the main sources of infection in sepsis is believed to be the intestinal microbiota via traslocation through the mucosa to the bloodstream. Systemic inflammation weakens intestinal barrier function (IBF) in animal models, resulting in increased bacterial traslocation [2]. Even if the management of sepsis has advanced in the last decades, mortality is still high and there are blanks in terms of pathological systems and long-term consequences. Thus, the search for effective treatments is clearly justified. Glucocorticoids (GC) are part of the drugs used in sepsis, but they have only shown a moderate therapeutic effect. This fact may be caused by harmful effects of GCs on IBF, whose compromise may limit GC clinical benefit by facilitating luminal translocation of microorganisms. Besides, GC treatment impairs epithelial healing in experimental colitis in mice [3]. Previous results of our research group have shown that mice with induced deletion of the GC receptor (GR) in intestinal epithelial cells (i.e. NR3C1ΔIEC mice) are protected against dextran sulphate sodium (DSS)-induced colitis [4]. In turn, gene deletion results in a short lived inflammatory response in the colon [5]. Objective: Understanding the role of the intestinal epithelial GR and its involvement in IBF regulation in experimental sepsis, with the ultimate goal of improving the management of sepsis with GCs. Matherial and methods: The cecal ligation and puncture (CLP) model of sepsis was applied to WT C57BL/6J and NR3C1ΔIEC mice. Ceacum-exposed mice were used as control (Sham). Mice were sacrificed 24 hours after surgery. Four hours before sacrifice, mice were administered 4 kD FITC-dextran, a fluorescent marker of permeability. Colon, jejunum, adrenes, kidney and liver RT-qPCRs were performed as well as determination of plasma FITC-dextran and corticosterone plasma levels. Results: After 24 h, CLP mice exhibited elevated corticosterone plasma levels with hypoglycemia and splenomegaly. Intestinal barrier function was weakened, as indicated by increased FITC-dextran plasma levels. A modest increase in inflammatory markers (S100a8, Cxcl1) was noted in the colon and jejunum. The expression of Tjp1, involved in barrier function, was downregulated in CLP mice. Similarly, the colonic expression of Cyp11a1 and Lrh1, involved in local steroidogenesis, was lower in CLP mice, regardless of genotype. Markers of inflammation were also augmented in the lung and kidney. CLP mice exhibited hypercorticosteronemia, which was associated to increased Cyp11a1 in the adrenes. Of note, both parameters were less pronounced in KO mice. The latter also exhibited dampened inflammatory response in the colon but not the jejunum. FITC-dextran plasma levels were similarly increased in WT and KO mice. Conclusions: In the early stages of the CLP model of sepsis the colon and jejunum are inflamed, and epithelial deletion of the glucocorticoid receptor appears to modulate inflammation in the former, with no change in barrier function. Further studies will characterize the microbiota composition and phenotype in later stages and in the response to glucocorticoid treatment

Geomicrobiological heterogeneity of lithic habitats in the extreme environment of Antarctic nunataks: a potential early Mars analog

Nunataks are permanent ice-free rocky peaks that project above ice caps in polar regions, thus being exposed to extreme climatic conditions throughout the year. They undergo extremely low temperatures and scarcity of liquid water in winter, while receiving high incident and reflected (albedo) UVA-B radiation in summer. Here, we investigate the geomicrobiology of the permanently exposed lithic substrates of nunataks from Livingston Island (South Shetlands, Antarctic Peninsula), with focus on prokaryotic community structure and their main metabolic traits. Contrarily to first hypothesis, an extensive sampling based on different gradients and multianalytical approaches demonstrated significant differences for most geomicrobiological parameters between the bedrock, soil, and loose rock substrates, which overlapped any other regional variation. Brevibacillus genus dominated on bedrock and soil substrates, while loose rocks contained a diverse microbial community, including Actinobacteria, Alphaproteobacteria and abundant Cyanobacteria inhabiting the milder and diverse microhabitats within. Archaea, a domain never described before in similar Antarctic environments, were also consistently found in the three substrates, but being more abundant and potentially more active in soils. Stable isotopic ratios of total carbon (δ 13C) and nitrogen (δ 15N), soluble anions concentrations, and the detection of proteins involved in key metabolisms via the Life Detector Chip (LDChip), suggest that microbial primary production has a pivotal role in nutrient cycling at these exposed areas with limited deposition of nutrients. Detection of stress-resistance proteins, such as molecular chaperons, suggests microbial molecular adaptation mechanisms to cope with these harsh conditions. Since early Mars may have encompassed analogous environmental conditions as the ones found in these Antarctic nunataks, our study also contributes to the understanding of the metabolic features and biomarker profiles of a potential Martian microbiota, as well as the use of LDChip in future life detection missions.This project has been funded by the Spanish Ministry of Science and Innovation (MICINN)/European Regional Development Fund (FEDER) project no. RTI2018-094368-B-I00; the European Research Council Consolidator grant no. 818602; and the Spanish State Research Agency (AEI) project no. MDM-2017-0737, Unidad de Excelencia “María de Maeztu” to Centro de Astrobiología

Mesonia oceanica sp. Nov., isolated from oceans during the tara oceans expedition, with a preference for mesopelagic waters

Strain ISS653T, isolated from Atlantic seawater, is a yellow pigmented, non-motile, Gram-reaction-negative rod-shaped bac-terium, strictly aerobic and chemoorganotrophic, slightly halophilic (1-15% NaCl) and mesophilic (4-37 °C), oxidase-and catalase-positive and proteolytic. Its major cellular fatty acids are iso-C15:0, iso-C15:0 2-OH, and iso-C17:0 3-OH; the major identified phospholipid is phosphatidylethanolamine and the major respiratory quinone is MK6. Genome size is 4.28 Mbp and DNA G+C content is 34.9 mol%. 16S rRNA gene sequence similarity places the strain among members of the family Flavobacteriaceae, with the type strains of Mesonia phycicola (93.2%), Salegentibacter mishustinae (93.1%) and Mesonia mobilis (92.9%) as closest relatives. Average amino acid identity (AAI) and average nucleotide identity (ANI) indices show highest values with M. mobilis (81% AAI; 78.9% ANI), M. phycicola (76% AAI; 76.3% ANI), Mesonia maritima (72% AAI, 74.9% ANI), Mesonia hippocampi (64% AAI, 70.8% ANI) and Mesonia algae (68% AAI; 72.2% ANI). Phylogenomic analysis using the Up-to-date-Bacterial Core Gene set (UBCG) merges strain ISS653T in a clade with species of the genus Mesonia. We conclude that strain ISS653T represents a novel species of the genus Mesonia for which we propose the name Mesonia oceanica sp. nov., and strain ISS653T (=CECT 9532T=LMG 31236T) as the type strain. A second strain of the species, ISS1889 (=CECT 30008) was isolated from Pacific Ocean seawater. Data obtained throughout the Tara oceans expedition indicate that the species is more abundant in the mesopelagic dark ocean than in the photic layer and it is more frequent in the South Pacific, Indian and North Atlantic oceans

El efecto del capital social en la violencia en la pareja adolescente desde una perspectiva de interseccionalidad

Antecedentes: La violencia de pareja (VP) es un fenómeno social complejo ampliamente estudiado. Sin embargo, pocos de estos estudios consideran el capital social y la interseccionalidad. Nuestro objetivo fue describir el efecto del capital social en la victimización por VP entre estudiantes de secundaria, considerando tres factores de interseccionalidad (sexo asignado al nacer, orientación sexual y origen migratorio) en dos ciudades de España en 2019-2020. Métodos: Se analizó una muestra transversal de 640 adolescentes con pareja, de 13 a 16 años, de un programa de promoción de relaciones equitativas en Alicante y Terrassa. La variable resultado fue haber sufrido violencia en la pareja (control, miedo, física o sexual) en algún momento de la vida. Se utilizaron variables individuales y relacionales para calcular la razón de prevalencia ajustada (RPa) a partir de modelos multivariable construidos mediante regresión robusta de Poisson, y estratificados por sexo, orientación sexual y origen migratorio. Resultados: La mayor prevalencia de VP (56,25%) se encontró en adolescentes lesbianas, gays o bisexuales (LGB) sin actividad social. La orientación LGB se asoció significativamente con la victimización por VP en los adolescentes con origen en países de bajos ingresos (PBI) (aPR: 1,93) y en las chicas (aPR: 1,53). Por último, la procedencia de un PBI fue un determinante significativo de la VP en los chicos (aPR: 1,76) y en los estudiantes, independientemente de la orientación sexual. Una mayor actividad social mostró un efecto protector para los estudiantes de origen en países de altos ingresos (PAI) y de orientación sexual LGB, independientemente del sexo y la orientación sexual. Conclusiones: La actividad social se asocia de forma desigual con una menor victimización por violencia de la pareja en algún momento de la vida adolescente. La fuerte asociación del origen migratorio y la orientación sexual con la VPI refuerza el enfoque de su prevención en los adolescentes desde una perspectiva de interseccionalidad.Background: Intimate partner violence (IPV) is a complex social phenomenon widely studied. However, few of these studies consider social capital and intersectionality. Our aim was to describe the effect of social capital in IPV victimisation among secondary students, considering three factors of intersectionality (sex assigned at birth, sexual orientation and migration background) in two cities of Spain in 2019-2020. Methods: We analysed a cross-sectional sample of 640 ever-partnered adolescents aged 13–16 years who had taken part in a programme for positive relationship. The main outcome was lifetime IPV (control, fear, physical or sexual violence). Individual and relational variables (bonding social capital) were used to calculate adjusted prevalence ratios (aPR) from multivariate Robust Poisson regression models stratified by sex assigned at birth, sexual orientation and migration background (factors of intersectionality). Results: The highest IPV prevalence (56.25%) was found in lesbian, gay or bisexual (LGB) adolescents without social activity. LGB orientation was significantly associated with IPV victimisation in adolescents with low-income country (LIC) backgrounds (aPR: 1.93) and in girls (1.53). Finally, a LIC background was a significant determinant of IPV in boys (aPR: 1.76) and in students independently of sexual orientation. Higher social activity showed a protective effect for students with HIC backgrounds and LGB-sexual orientations. A possible protective effect of social support in HIC backgrounds and regardless of sex and sexual orientation must be considered. Conclusions: Social activity is unequally associated with less lifetime IPV. The strong association of migration background and sexual orientation with IPV reinforces the approach to its prevention in adolescents from an intersectionality perspective.This study was funded by the multi-centre project "Promotion of protective assets against gender violence in adolescence and preadolescence" (Grant reference PI18/00590 and PI18/00544) of the Carlos III Institute of Health, as an intermediary body of the European Regional Development Fund (ERDF) and the European Social Fund (ESF). The project was also supported by the Centro de Investigación Biomédica en Red de Epidemiología Salud Pública (CIBERESP) through the subprogramme on Prevention of Gender Violence of the Programme on Social Determinants of Health (no grant award number specified)

Estudio comparativo de la composición química de los aceites esenciales de aloysia triphylla l´her britton cultivada en diferentes regiones de colombia

Se compararon las composiciones químicas de aceites esenciales (AEs) de plantas de Aloysia triphylla, recolectadas en tres regiones de Colombia, en tres estados de madurez. Los AEs fueron obtenidos por hidrodestilación asistida por la radiación de microondas (MWHD) y analizados por GC-MS. El componente mayoritario presente en los AEs evaluados fue el citral (32-41 por ciento). El mayor rendimiento ( por cientop/p) se obtuvo para las plantas recolectadas en Bolívar, Santander (0.88 por ciento). La mayor acumulación de metabolitos de interés se logró para las plantas con dos meses de edad y antes de floración

Estudio comparativo de la composición química de los aceites esenciales de aloysia triphylla l´her britton cultivada en diferentes regiones de colombia

Se compararon las composiciones químicas de aceites esenciales (AEs) de plantas de Aloysia triphylla, recolectadas en tres regiones de Colombia, en tres estados de madurez. Los AEs fueron obtenidos por hidrodestilación asistida por la radiación de microondas (MWHD) y analizados por GC-MS. El componente mayoritario presente en los AEs evaluados fue el citral (32-41 por ciento). El mayor rendimiento ( por cientop/p) se obtuvo para las plantas recolectadas en Bolívar, Santander (0.88 por ciento). La mayor acumulación de metabolitos de interés se logró para las plantas con dos meses de edad y antes de floración