Implementación de la metodología Flipped Classroom en prácticas de análisis de datos: evaluación de satisfacción y rendimiento académico del estudiante

Memoria ID-0144. Ayudas de la Universidad de Salamanca para la innovación docente, curso 2016-2017

Bee trypanosomatids: first steps in the analysis of the fenetic variation and population structure of Lotmaria passim, Crithidia bombi and Crithidia mellificae

Trypanosomatids are among the most prevalent parasites in bees but, despite the fact that their impact on the colonies can be quite important and that their infectivity may potentially depend on their genotypes, little is known about the population diversity of these pathogens. Here we cloned and sequenced three non-repetitive single copy loci (DNA topoisomerase II, glyceraldehyde-3-phosphate dehydrogenase and RNA polymerase II large subunit, RPB1) to produce new genetic data from Crithidia bombi, C. mellificae and Lotmaria passim isolated from honeybees and bumblebees. These were analysed by applying population genetic tools in order to quantify and compare their variability within and between species, and to obtain information on their demography and population structure. The general pattern for the three species was that they were subject to the action of purifying selection on nonsynonymous variants, the levels of within species diversity were similar irrespective of the host, there was evidence of recombination among haplotypes and they showed no haplotype structuring according to the host. C. bombi exhibited the lowest levels of synonymous variation (πS= 0.06 ± 0.04%) — and a mutation frequency distribution compatible with a population expansion after a bottleneck — that contrasted with the extensive polymorphism displayed by C. mellificae (πS= 2.24 ± 1.00 %), which likely has a more ancient origin. L. passim showed intermediate values (πS= 0.40 ± 0.28 %) and an excess of variants a low frequencies probably linked to the spread of this species to new geographical areasThis study was supported by the Ministerio de Economía y Competitividad (MINECO) (grant number CGL2012-34897), the Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) - European Regional Development Fund (ERDF) (grant numbers E-RTA2014-00003-C03-01, 02 and 03), the Eva Crane Trust (grant number ECTA_20210308) and the Fundación Séneca - Agencia de Ciencia y Tecnología de la Región de Murcia (grant of Regional Excellence19908/GERM/2015)S

Difusión de los resultados de las investigaciones realizadas por los estudiantes de grado en las asignaturas de Metodología de Investigación

Memoria ID-113. Ayudas de la Universidad de Salamanca para la innovación docente, curso 2018-2019

Schnurri-3 drives tumor growth and invasion in cancer cells expressing interleukin-13 receptor alpha 2

Interleukin 13 receptor alpha 2 (IL13Rα2) is a relevant therapeutic target in glioblastoma (GBM) and other tumors associated with tumor growth and invasion. In a previous study, we demonstrated that protein tyrosine phosphatase 1B (PTP1B) is a key mediator of the IL-13/IL13Rα2 signaling pathway. PTP1B regulates cancer cell invasion through Src activation. However, PTP1B/Src downstream signaling mechanisms that modulate the invasion process remain unclear. In the present research, we have characterized the PTP1B interactome and the PTP1B-associated phosphoproteome after IL-13 treatment, in different cellular contexts, using proteomic strategies. PTP1B was associated with proteins involved in signal transduction, vesicle transport, and with multiple proteins from the NF-κB signaling pathway, including Tenascin-C (TNC). PTP1B participated with NF-κB in TNC-mediated proliferation and invasion. Analysis of the phosphorylation patterns obtained after PTP1B activation with IL-13 showed increased phosphorylation of the transcription factor Schnurri-3 (SHN3), a reported competitor of NF-κB. SHN3 silencing caused a potent inhibition in cell invasion and proliferation, associated with a down-regulation of the Wnt/β-catenin pathway, an extensive decline of MMP9 expression and the subsequent inhibition of tumor growth and metastasis in mouse models. Regarding clinical value, high expression of SHN3 was associated with poor survival in GBM, showing a significant correlation with the classical and mesenchymal subtypes. In CRC, SHN3 expression showed a preferential association with the mesenchymal subtypes CMS4 and CRIS-B. Moreover, SHN3 expression strongly correlated with IL13Rα2 and MMP9-associated poor prognosis in different cancers. In conclusion, we have uncovered the participation of SNH3 in the IL-13/IL13Rα2/PTP1B pathway to promote tumor growth and invasion. These findings support a potential therapeutic value for SHN3.Angela Martín-Regalado was supported by an FPU fellowship (FPU18/05766-MEFP). Laura Pintado-Berninches was supported by a Margarita Salas contract (CA1/RSUE/2021-00208) from the Ministry of Universities (Spain). Javier Robles and Issam Boukich were supported by IND2019/BMD-17153 and IND2022/BMD-23554 fellow ships of the Comunidad de Madrid. This project was supported by grants RTI2018-095055-B-I00, PID2021-122227OB-I00 and CPP2021-008337 from the MCIN/AEI/10.13039/501100011033 using Next Generation EU/PRTR funds to JIC, and PI21CIII/00002 from the MCIN and FEDER funds to PSG and NINDS R01 NS122395 to IVB.S

Gamificación en la universidad: diseño, desarrollo y evaluación del empleo de Kahoot en el aula. Satisfacción y rendimiento académico de los estudiantes en las asignaturas de metodología de investigación

Memoria ID-0123. Ayudas de la Universidad de Salamanca para la innovación docente, curso 2017-2018

SARS-CoV-2 Infection and C-Section: A Prospective Observational Study

Pregnant women are particularly vulnerable to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. In addition to unfavorable perinatal outcomes, there has been an increase in obstetric interventions. With this study, we aimed to clarify the reasons, using Robson's classification model, and risk factors for cesarean section (C-section) in SARS-CoV-2-infected mothers and their perinatal results. This was a prospective observational study that was carried out in 79 hospitals (Spanish Obstetric Emergency Group) with a cohort of 1704 SARS-CoV-2 PCR-positive pregnant women that were registered consecutively between 26 February and 5 November 2020. The data from 1248 pregnant women who delivered vaginally (vaginal + operative vaginal) was compared with those from 456 (26.8%) who underwent a C-section. C-section patients were older with higher rates of comorbidities, in vitro fertilization and multiple pregnancies (p < 0.05) compared with women who delivered vaginally. Moreover, C-section risk was associated with the presence of pneumonia (p < 0.001) and 41.1% of C-sections in patients with pneumonia were preterm (Robson's 10th category). However, delivery care was similar between asymptomatic and mild-moderate symptomatic patients (p = 0.228) and their predisposing factors to C-section were the presence of uterine scarring (due to a previous C-section) and the induction of labor or programmed C-section for unspecified obstetric reasons. On the other hand, higher rates of hemorrhagic events, hypertensive disorders and thrombotic events were observed in the C-section group (p < 0.001 for all three outcomes), as well as for ICU admission. These findings suggest that this type of delivery was associated with the mother's clinical conditions that required a rapid and early termination of pregnancy.This project was supported by public funds that were obtained in competitive calls: Grant COV20/00021 (EUR 43,000 from the Instituto de Salud Carlos III-Spanish Ministry of Health) and co-financed with Fondo Europeo de Desarrollo Regional (FEDER) funds

Conocimiento, conducta y actitud ante el Código europeo contra el cáncer por parte de los profesionales sanitarios de atención primaria

Objetivo: Determinar si los profesionales de atención primaria (AP) conocen el Código europeo contra el cáncer (CECC) y si ello se relaciona con que practiquen sus recomendaciones. Diseño: Observacional descriptivo. Emplazamiento: Centros de AP españoles. Participantes: Profesionales de AP. Intervenciones: Cuestionario autoadministrado. Mediciones principales: Factores de riesgo y protectores del cáncer, pruebas de cribado y conocimiento del CECC. Resultados: Respondieron 1.734 profesionales (10, 5%), edad media de 48, 5 años (DT: 9, 2), el 71, 5% mujeres. El 50, 0% desconoce el CECC; los tutores (OR = 1, 61), adscritos al PAPPS (OR = 1, 51) y que llevan más tiempo trabajando en AP (OR = 2, 62) son los que más lo conocen. El 7, 2% fuma y el 79, 1% bebe alcohol. El 64, 1% presenta normopeso y el 19% realiza actividad física. Evita exponerse al sol el 52, 7% y usa protectores el 53, 8%. El 85, 2% nunca se ha realizado un cribado colorrectal, el 11% nunca una citología y el 12% una mamografía. Existe relación entre conocer el CECC y el tabaquismo (p < 0, 001), sobrepeso/obesidad (p = 0, 024), actividad física (p = 0, 003), ingesta de carnes (p < 0, 001), mamografía (p < 0, 001), citología (p = 0, 022), PSA (p = 0, 045), así como el fomento de sus consejos entre los pacientes. Conclusiones: El CECC es desconocido por la mitad de los profesionales de AP. La práctica de sus recomendaciones es desigual, siendo la prevalencia de tabaquismo baja, y la protección solar una conducta poco extendida. Los que lo conocen son los que más practican sus recomendaciones. Objective: To decide if Primary Care (PC) professionals know the European Code against Cancer (CECC) and if this relates to practice the recommendations with themselves and with their patients. Design: descriptive, observational study. Setting: Spanish Health Centers. Participants: Health professionals. Interventions: self-administered questionnaire. Main measurements: cancer protective and risk factors, screening for colorectal, breast, cervix and prostate cancer, level of knowledge of the ECAC. Results: 1734 participants (10.5%), mean age 47.4 years (SD: 8.6), 71.5% female. 50.0% do not know the CECC; tutors (OR = 1.61), assigned to the PAPPS (OR = 1.51) and who have been working in AP for more time (OR = 2.62) are the ones who know it most. 7.2% smoke and 79.1% drink alcohol. 64.1% presented normal weight and 19% performed physical activity. They avoid exposing themselves to the sun 52.7% and use protectors 53.8%. 85.2% have never had a colorectal screening, 11% never had a cytology, and 12% had a mammogram. There is a relationship between knowing the CECC and smoking (P <.001), overweight/obesity (P =.024), physical activity (P =.003), meat intake (P <.001), mammography (P <.001), cytology (P =.022), PSA (P =.045), as well as the promotion of their advice among patients. Conclusions: The CECC is unknown by half of the AP professionals. The practice of its recommendations is uneven, with the prevalence of low smoking, and sun protection a little extended behavior. Those who know him are the ones who most practice his recommendations

Study on the Acceptability of an ICT Platform for Older Adults with Mild Cognitive Impairment

EhcoBUTLER is an Information and Communication Technology (ICT) solution funded by the EuropeanUnion (H2020; ID: 643566)and intended especially for elderly people with mild cognitive impairment (MCI) to improve their health, independence and quality oflife, particularly at the social level. The purpose of this study is to assess the acceptability of ehcoBUTLER based on a survey deliveredto potential users and actors involved in their care, exploring theirexpectations and preferences, while anticipating the system'sfunctional requirements. The survey was delivered online to 313participants (11% end users, 25% informal caregivers, 48% formalcaregivers and 16% administration/management staff) from eight countries. Participants rated the different functionalities ofehcoBUTLER positively, 86.1% perceiving it as an interesting and useful system. Likewise, they assessed it as a commerciallyattractive product (75.1%). End users expressed a stronger preference for the social module. Nevertheless, they would be ready topay a low monthly price for ehcoBUTLER. Professionals would be willing to pay choosing its functionalities modularly, but theywould also expect it to be funded by the National Health System, centres or businesses. The conclusion is that all participants foundehcoBUTLER interesting, useful and ergonomic. However, to effectively implement it, it is necessary to bridge the digital gap andaddress the issue of insufficient investment in products aimed atolder adults with cognitive impairment. To supplement cognitivetraining systems with social, emotional or entertainment functionalities could improve adherence to their use

Signal como aula virtual

Memoria ID-053 Ayudas de la Universidad de Salamanca para la innovación docente, curso 2020-2021

Transformando enseñanzas presenciales en semipresenciales: un ejemplo, la especialidad de Formación y Orientación Laboral en el Máster en Formación del Profesorado. Protocolo de actuación, evaluación y nuevos diseños para la docencia universitaria

Fac. de Ciencias Políticas y SociologíaFALSEsubmitte