Biospectroscopy of Nanodiamond-Induced Alterations in Conformation of Intra- and Extracellular Proteins: A Nanoscale IR Study

The toxicity of nanomaterials raises major concerns because of the impact that nanomaterials may have on health, which remains poorly understood. We need to explore the fate of individual nanoparticles in cells at nano and molecular levels to establish their safety. Conformational changes in secondary protein structures are one of the main indicators of impaired biological function and hence, the ability to identify these changes at a nanoscale level offers unique insights into the nanotoxicity of materials. Here, we used nanoscale infrared spectroscopy and demonstrated for the first time that nanodiamonds induced alterations in both extra- and intracellular secondary protein structures, leading to the formation of antiparallel β-sheet, β-turns, intermolecular β- sheet and aggregation of proteins. These conformational changes of the protein structure may result in the loss of functionality of proteins and in turn lead to adverse effects

Asynchronous warming and δ18O evolution of deep Atlantic water masses during the last deglaciation

Author Posting. © The Author(s), 2017. This is the author's version of the work. It is posted here under a nonexclusive, irrevocable, paid-up, worldwide license granted to WHOI. It is made available for personal use, not for redistribution. The definitive version was published in Proceedings of the National Academy of Sciences of the United States of America 114 (2017): 11075-11080, doi: 10.1073/pnas.1704512114.The large-scale reorganization of deep-ocean circulation in the Atlantic involving changes in North Atlantic Deep Water (NADW) and Antarctic Bottom Water (AABW) played a critical role in regulating hemispheric and global climate during the last deglaciation. However, changes in the relative contributions of NADW and AABW and their properties are poorly constrained by marine records, including δ18O of benthic foraminiferal calcite (δ18Oc). Here we use an isotope-enabled ocean general circulation model with realistic geometry and forcing conditions to simulate the deglacial water mass and δ18O evolution. Model results suggest that in response to North Atlantic freshwater forcing during the early phase of the last deglaciation, NADW nearly collapses while AABW mildly weakens. Rather than reflecting changes in NADW or AABW properties due to freshwater input as suggested previously, the observed phasing difference of deep δ18Oc likely reflects early warming of the deep northern North Atlantic by ~1.4°C while deep Southern Ocean temperature remains largely unchanged. We propose a thermodynamic mechanism to explain the early warming in the North Atlantic, featuring a strong mid-depth warming and enhanced downward heat flux via vertical mixing. Our results emphasize that the way ocean circulation affects heat, a dynamic tracer, is considerably different than how it affects passive tracers like δ18O, and call for caution when inferring water mass changes from δ18Oc records while assuming uniform changes in deep temperatures.This work is supported by the U.S. NSF P2C2 projects (1401778 and 1401802) and OCE projects (1600080 and 1566432), China NSFC 41630527, and the Wisconsin Alumni Research Foundatio

Investigating the Direct Meltwater Effect in Terrestrial Oxygenâ Isotope Paleoclimate Records Using an Isotopeâ Enabled Earth System Model

Variations in terrestrial oxygenâ isotope reconstructions from ice cores and speleothems have been primarily attributed to climatic changes of surface air temperature, precipitation amount, or atmospheric circulation. Here we demonstrate with the fully coupled isotopeâ enabled Community Earth System Model an additional process contributing to the oxygenâ isotope variations during glacial meltwater events. This process, termed â the direct meltwater effect,â involves propagating large amounts of isotopically depleted meltwater throughout the hydrological cycle and is independent of climatic changes. We find that the direct meltwater effect can make up 15â 35% of the δ18O signals in precipitation over Greenland and eastern Brazil for large freshwater forcings (0.25â 0.50 sverdrup (106 m3/s)). Model simulations further demonstrate that the direct meltwater effect increases with the magnitude and duration of the freshwater forcing and is sensitive to both the location and shape of the meltwater. These new modeling results have important implications for past climate interpretations of δ18O.Key PointsA portion of the δ18O signal in landâ based paleoclimate proxies can be attributed to the direct meltwater effect instead of climatic changesThe direct meltwater effect can make up 15â 35% of the δ18O signals in precipitation in Greenland and eastern Brazil for large meltwater eventsThe direct meltwater effect increases with the magnitude and duration of the freshwater forcing and is sensitive to location and shape dependentPeer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/141374/1/grl56782_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/141374/2/grl56782-sup-0001-Supporting_Information.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/141374/3/grl56782.pd

Early to Late Holocene Surface Exposure Ages From Two Marine-Terminating Outlet Glaciers in Northwest Greenland

Terrestrial chronologies from southern Greenland provide a detailed deglacial history of the Greenland Ice Sheet (GIS). The northern GIS margin history, however, is less established. Here we present surface exposure ages from moraines associated with two large outlet glaciers, Petermann and Humboldt, in the northwestern sector of the GIS. These moraine chronologies indicate a Little Ice Age advance of the ice sheet margin before similar to 0.3 ka and a possible equivalent advance of similar magnitude prior to similar to 2.8 ka. An early Holocene moraine at Humboldt Glacier was abandoned by 8.3 +/- 1.7 ka and is contemporaneous with other moraines deposited along the entire western GIS margin. This widespread ice margin stability between similar to 9 and 8 ka indicates that while this margin was influenced by warming atmospheric temperatures during the early Holocene, the warming was likely overprinted with the effect of the abrupt climate cooling at 9.3 and 8.2 ka. Plain Language Summary The global climate is warming, and the Greenland Ice Sheet is responding. A more complete understanding of this process is needed to better predict its future response to climate change. We determine how the ice sheet changed following the last ice age in northwest Greenland. The northwest sector of the ice sheet retreated to the coast by similar to 10,000 years ago during a period of warming atmospheric temperatures. About 8,300 years ago the ice stopped retreating despite relatively high atmospheric temperatures. A similar standstill occurred in areas along western Greenland between similar to 9,000 and 8,000 years ago. This suggests that despite the long-term warming, well-known abrupt cooling events that occurred in the region at this time influenced the ice sheet margin and temporarily stopped the long-term pattern of ice retreat. The ice sheet retreated after 8,300 years ago and then advanced during the latest cold period, the Little Ice Age (similar to 350-1850 CE), in a fashion similar to elsewhere in Greenland. Our study finds that the Greenland Ice Sheet margins are sensitive to both long-term (>1,000 years) and short-term (<100 years) atmospheric temperature changes. This sensitivity of the ice margin has important implications when assessing ongoing and future ice loss today

Southwest Atlantic water mass evolution during the last deglaciation

The rise in atmospheric CO2 during Heinrich Stadial 1 (HS1; 14.5–17.5 kyr B.P.) may have been driven by the release of carbon from the abyssal ocean. Model simulations suggest that wind‐driven upwelling in the Southern Ocean can liberate 13C‐depleted carbon from the abyss, causing atmospheric CO2 to increase and the δ13C of CO2 to decrease. One prediction of the Southern Ocean hypothesis is that water mass tracers in the deep South Atlantic should register a circulation response early in the deglaciation. Here we test this idea using a depth transect of 12 cores from the Brazil Margin. We show that records below 2300 m remained 13C‐depleted until 15 kyr B.P. or later, indicating that the abyssal South Atlantic was an unlikely source of light carbon to the atmosphere during HS1. Benthic δ18O results are consistent with abyssal South Atlantic isolation until 15 kyr B.P., in contrast to shallower sites. The depth dependent timing of the δ18O signal suggests that correcting δ18O for ice volume is problematic on glacial terminations. New data from 2700 to 3000 m show that the deep SW Atlantic was isotopically distinct from the abyss during HS1. As a result, we find that mid‐depth δ13C minima were most likely driven by an abrupt drop in δ13C of northern component water. Low δ13C at the Brazil Margin also coincided with an ~80‰ decrease in Δ14C. Our results are consistent with a weakening of the Atlantic meridional overturning circulation and point toward a northern hemisphere trigger for the initial rise in atmospheric CO2 during HS1.Key PointsDeep SW Atlantic was unlikely source of light carbon to atmosphere during HS1Mid‐depth isotopic anomalies due to change in northern component waterNorthern component water had robust influence in South Atlantic during HS1Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/111970/1/palo20190.pd

Activation of D2 dopamine receptor-expressing neurons in the nucleus accumbens increases motivation.

Striatal dopamine receptor D1-expressing neurons have been classically associated with positive reinforcement and reward, whereas D2 neurons are associated with negative reinforcement and aversion. Here we demonstrate that the pattern of activation of D1 and D2 neurons in the nucleus accumbens (NAc) predicts motivational drive, and that optogenetic activation of either neuronal population enhances motivation in mice. Using a different approach in rats, we further show that activating NAc D2 neurons increases cue-induced motivational drive in control animals and in a model that presents anhedonia and motivational deficits; conversely, optogenetic inhibition of D2 neurons decreases motivation. Our results suggest that the classic view of D1-D2 functional antagonism does not hold true for all dimensions of reward-related behaviours, and that D2 neurons may play a more prominent pro-motivation role than originally anticipated.A special acknowledgement to Karl Deisseroth from Stanford University, for providing viral constructs and for comments on the manuscript, and to Alan Dorval from the University of Utah, for providing mouse strains. Thanks to Luis Jacinto, Joao Oliveira and Joana Silva that helped in some technical aspects of the experiments. C.S.-C., B.C., A.D.-P. and S.B. are recipients of Fundacao para a Ciencia e Tecnologia (FCT) fellowships (SFRH/BD/51992/2012; SFRH/BD/98675/2013; SFRH/BD/90374/2012; SFRH/BD/89936/2012). A.J.R. is a FCT Investigator (IF/00883/2013). This work was co-financed by the Portuguese North Regional Operational Program (ON.2 - O Novo Norte) under the National Strategic Reference Framework (QREN), through the European Regional Development Fund (FEDER). Part of the work was supported by the Janssen Neuroscience Prize (1st edition).info:eu-repo/semantics/publishedVersio

Europe's lost forests: a pollen-based synthesis for the last 11,000 years

8000 years ago, prior to Neolithic agriculture, Europe was mostly a wooded continent. Since then, its forest cover has been progressively fragmented, so that today it covers less than half of Europe’s land area, in many cases having been cleared to make way for fields and pasture-land. Establishing the origin of Europe’s current, more open land-cover mosaic requires a long-term perspective, for which pollen analysis offers a key tool. In this study we utilise and compare three numerical approaches to transforming pollen data into past forest cover, drawing on >1000 14C-dated site records. All reconstructions highlight the different histories of the mixed temperate and the northern boreal forests, with the former declining progressively since ~6000 years ago, linked to forest clearance for agriculture in later prehistory (especially in northwest Europe) and early historic times (e.g. in north central Europe). In contrast, extensive human impact on the needle-leaf forests of northern Europe only becomes detectable in the last two millennia and has left a larger area of forest in place. Forest loss has been a dominant feature of Europe’s landscape ecology in the second half of the current interglacial, with consequences for carbon cycling, ecosystem functioning and biodiversity

Global cooling as a driver of diversification in a major marine clade

Climate is a strong driver of global diversity and will become increasingly important as human influences drive temperature changes at unprecedented rates. Here we investigate diversification and speciation trends within a diverse group of aquatic crustaceans, the Anomura. We use a phylogenetic framework to demonstrate that speciation rate is correlated with global cooling across the entire tree, in contrast to previous studies. Additionally, we find that marine clades continue to show evidence of increased speciation rates with cooler global temperatures, while the single freshwater clade shows the opposite trend with speciation rates positively correlated to global warming. Our findings suggest that both global cooling and warming lead to diversification and that habitat plays a role in the responses of species to climate change. These results have important implications for our understanding of how extant biota respond to ongoing climate change and are of particular importance for conservation planning of marine ecosystems

Holocene break-up and reestablishment of the Petermann Ice Tongue, Northwest Greenland

Over the last decade, two major calving events of the Petermann Ice Tongue in Northwest Greenland have led to speculation on its future stability and contribution to further Greenland Ice Sheet mass loss. However, it has been unclear if these events are anomalous or typical within the context of limited historical observations. We extend the historical record of the floating ice tongue using the stratigraphy of Petermann Fjord sediments to provide a longer-term perspective. Computed tomography (CT) scans, X-Ray Fluorescence (XRF) scans, Ice-Rafted Debris (IRD) counts, and the magnetic properties of specific particle size fractions constrain changes in depositional processes and sediment sources at our core sites, allowing for reconstructions of past behavior of the Petermann Ice Tongue. Radiocarbon dating of foraminifera, 210Pb, and paleomagnetic secular variation (PSV) provide age control and help to address uncertainties in radiocarbon reservoir ages. A floating ice tongue in Petermann Fjord formed in late glacial time as Petermann Glacier retreated from an advanced grounded position. This paleo-ice tongue broke-up during the early Holocene when high northern latitude summer insolation was higher than present. After gradual regrowth of the ice tongue associated with regional cooling, the ice tongue reached its historical extent only within the last millennium. Little or no ice tongue was present for nearly 5000 years during the middle Holocene, when decadal mean regional temperatures are estimated to be 0.8e2.9 °C higher than preindustrial (1750 CE) and seasonal sea-ice in the Lincoln Sea was reduced. This pre-historical behavior shows that recent anthropogenic warming may already be in the range of ice tongue instability and future projected warming increases the risk of ice tongue break-up by the mid- 21st Century

The intertropical convergence zone modulates intense hurricane strikes on the western North Atlantic margin

© The Author(s), 2016. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Scientific Reports 6 (2016): 21728, doi:10.1038/srep21728Most Atlantic hurricanes form in the Main Development Region between 9°N to 20°N along the northern edge of the Intertropical Convergence Zone (ITCZ). Previous research has suggested that meridional shifts in the ITCZ position on geologic timescales can modulate hurricane activity, but continuous and long-term storm records are needed from multiple sites to assess this hypothesis. Here we present a 3000 year record of intense hurricane strikes in the northern Bahamas (Abaco Island) based on overwash deposits in a coastal sinkhole, which indicates that the ITCZ has likely helped modulate intense hurricane strikes on the western North Atlantic margin on millennial to centennial-scales. The new reconstruction closely matches a previous reconstruction from Puerto Rico, and documents a period of elevated intense hurricane activity on the western North Atlantic margin from 2500 to 1000 years ago when paleo precipitation proxies suggest that the ITCZ occupied a more northern position. Considering that anthropogenic warming is predicted to be focused in the northern hemisphere in the coming century, these results provide a prehistoric analog that an attendant northern ITCZ shift in the future may again return the western North Atlantic margin to an active hurricane interval.This research was supported by NSF Awards: OCE-1519578, OCE-1356708, BCS-1118340