A simple integrated single-atom detector

We present a reliable and robust integrated fluorescence detector capable of detecting single atoms. The detector consists of a tapered lensed single-mode fiber for precise delivery of excitation light and a multimode fiber to collect the fluorescence. Both are mounted in lithographically defined SU-8 holding structures on an atom chip. Rb87 atoms propagating freely in a magnetic guide are detected with an efficiency of up to 66%, and a signal-to-noise ratio in excess of 100 is obtained for short integration times.Comment: 3 pages, 3 figure

MicroRNA 19a replacement partially rescues fin and cardiac defects in zebrafish model of Holt Oram syndrome

Holt-Oram Syndrome (HOS) is an autosomal dominant heart-hand syndrome caused by mutations in the TBX5 gene, a transcription factor capable of regulating hundreds of cardiac-specific genes through complex transcriptional networks. Here we show that, in zebrafish, modulation of a single miRNA is sufficient to rescue the morphogenetic defects generated by HOS. The analysis of miRNA-seq profiling revealed a decreased expression of miR-19a in Tbx5-depleted zebrafish embryos compared to the wild type. We revealed that the transcription of the miR-17-92 cluster, which harbors miR-19a, is induced by Tbx5 and that a defined dosage of miR-19a is essential for the correct development of the heart. Importantly, we highlighted that miR-19a replacement is able to rescue cardiac and pectoral fin defects and to increase the viability of HOS zebrafish embryos. We further observed that miR-19a replacement shifts the global gene expression profile of HOS-like zebrafish embryos towards the wild type condition, confirming the ability of miR-19a to rescue the Tbx5 phenotype. In conclusion our data demonstrate the importance of Tbx5/miR-19a regulatory circuit in heart development and provide a proof of principle that morphogenetic defects associated with HOS can be rescued by transient miRNA modulation

X-ray Emission from the Binary Central Stars of the Planetary Nebulae HFG 1, DS 1, and LoTr 5

Close binary systems undergoing mass transfer or common envelope interactions can account for the morphological properties of some planetary nebulae. The search for close binary companions in planetary nebulae is hindered by the difficulty of detecting cool, late-type, main sequence companions in binary systems with hot pre-white dwarf primaries. However, models of binary PN progenitor systems predict that mass accretion or tidal interactions can induce rapid rotation in the companion, leading to X-ray-emitting coronae. To test such models, we have searched for, and detected, X-ray emission from three binary central stars within planetary nebulae: the post-common envelope close binaries in HFG 1 and DS 1 consisting of O-type subdwarfs with late-type, main sequence companions, and the binary system in LoTr 5 consisting of O-type subdwarf and rapidly rotating, late-type giant companion. The X-ray emission in each case is best characterized by spectral models consisting of two optically-thin thermal plasma components with characteristic temperatures of about 10 MK and 15-40 MK, and total X-ray luminosities about 10^30 erg/s. We consider the possible origin of the X-ray emission from these binary systems and conclude that the most likely origin is, in each case, a corona around the late-type companion, as predicted by models of interacting binaries.Comment: tell your friend

Venture capital investments during Covid-19: evidence from the U.S. market

This paper studies the impact of Covid-19on the Venture Capital industry in the United States. Using a sample of 8,802 funding rounds, different univariate analyses and multiple regressions were conducted. The number of investments was not significantly affected, while companies received on average a higher round amount compared to the pre-pandemic period. This effect is mainly driven by later stage investments, leading to the conclusion that financing stages are impacted differently. Finally, the results show that during the crisis, lead Venture Capitalists tend to invest more in early stage companies operating in their core sector within first rounds

Effect Of Priming On Seed Vigor Of Wheat (Triticum Aestivum L.)

Priming is a process that controls the process of hydration of seeds for the ongoing metabolic processes before germination. Research on priming was conducted at ICERI seed laboratory from May to September 2009 to evaluate the effect of different priming methods on wheat seed vigor. Physical properties and chemical composition of seed were evaluated before seeds were treated. The priming treatment were conducted by soaking 250 g of seed in 500 mL of solution for hydropriming and halopriming. Two seed lots of Nias and Dewata variety were subjected to heated and unheated distilled water for 12hours and subjected to KCl and CaCl2 at 10, 20, and 30 ppm and unprimed seed. The experiment were arranged in completely randomized design, replicated thrice. Vigor evaluation by observed seed germination, simultaneity growth, germination rate, seedling dry weight, electric conductivity of seed leakage and length of primary root. The results showed that highest germination, simultaneity growth, seedling dry weight, and length of primary root, were priming treatment with KCl 30 ppm and CaCl2 20 and 30 ppm. Priming with distilled water for 12 hours gave higher germination percentage and simultaneity growth

Discovering miRNA Regulatory Networks in Holt–Oram Syndrome Using a Zebrafish Model

MicroRNAs (miRNAs) are small non-coding RNAs that play an important role in the post-transcriptional regulation of gene expression. miRNAs are involved in the regulation of many biological processes such as differentiation, apoptosis, and cell proliferation. miRNAs are expressed in embryonic, postnatal, and adult hearts, and they have a key role in the regulation of gene expression during cardiovascular development and disease. Aberrant expression of miRNAs is associated with abnormal cardiac cell differentiation and dysfunction. Tbx5 is a member of the T-box gene family, which acts as transcription factor involved in the vertebrate heart development. Alteration of Tbx5 level affects the expression of hundreds of genes. Haploinsufficiency and gene duplication of Tbx5 are at the basis of the cardiac abnormalities associated with Holt-Oram syndrome (HOS). Recent data indicate that miRNAs might be an important part of the regulatory circuit through which Tbx5 controls heart development. Using high-throughput technologies, we characterized genome-widely the miRNA and mRNA expression profiles in WT- and Tbx5-depleted zebrafish embryos at two crucial developmental time points, 24 and 48 h post fertilization (hpf). We found that several miRNAs, which are potential effectors of Tbx5, are differentially expressed; some of them are already known to be involved in cardiac development and functions, such as miR-30, miR-34, miR-190, and miR-21. We performed an integrated analysis of miRNA expression data with gene expression profiles to refine computational target prediction approaches by means of the inversely correlation of miRNA-mRNA expressions, and we highlighted targets, which have roles in cardiac contractility, cardiomyocyte proliferation/apoptosis, and morphogenesis, crucial functions regulated by Tbx5. This approach allowed to discover complex regulatory circuits involving novel miRNAs and protein coding genes not considered before in the HOS such as miR-34a and miR-30 and their targets.</p

Luces en una crisis global

El segundo semestre de 2008 fue especialmente difícil, en donde destacaron diversos puntos que se recogen en este número de Análisis Plural, como: los secuestros y el “ya basta”; los efectos de la crisis en México; el narcotráfico, terrorismo y corrupción; la “inacabada” reforma energética; el “avionazo” de Mouriño; la crisis en el Partido de la Revolución Democrática; el estallido bursátil estadounidense, de resonancia mundial, y los problemas energéticos agudizados por la globalización

automatic shape optimization of structural components with manufacturing constraints

Abstract Among optimization procedures, mesh morphing gained a relevant position: it proved to be a suitable tool in obtaining weight and stress concentration reduction, without the need to iterate the numerical model generation. Shape modification through mesh morphing can be performed in an automatic fashion adopting two approaches: defining parameters which will describe the modified shape or exploiting results coming from numerical analyses. With this second approach, it is possible to achieve a very high automation grade: stress values retrieved on component surfaces can be successfully employed to drive the shape modification of the component itself. This 'driven-by-numerical-results' automatic approach can lead to complex optimized shapes, which can be easily achieved with modern additive manufacturing processes, but not adopting traditional manufacturing processes. In the present work a method to include manufacturing constraints in a shape optimization workflow is presented and applied to different structural optimization cases, in order to demonstrate how even manufacturing based on traditional processes can take advantage of automatic shape optimization of structural components