Contribución al establecimiento de un modelo de estudio de imprinting y reprogramación basado en el cultivo de células haploides y transferencia nuclear en pez cebra

La existencia del mecanismo de imprinting en pez cebra puede ser útil para estudiar el papel que cumple este mismo fenómeno en mamíferos. El uso de cultivos celulares derivados de larvas haploides casi totalmente diferenciadas en experimentos de transplante nuclear de células somáticas, permitiría evaluar mejor la capacidad del ovocito para reprogramar el núcleo de dichas células.Santos Merino, MDC. (2011). Contribución al establecimiento de un modelo de estudio de imprinting y reprogramación basado en el cultivo de células haploides y transferencia nuclear en pez cebra. http://hdl.handle.net/10251/15891Archivo delegad

Assessment of Malnutrition in Heart Failure and Its Relationship with Clinical Problems in Brazilian Health Services.

Malnutrition in heart failure (HF) is frequent and associated with a worse prognosis. Due to differences in investment and the profile of those assisted, the objective of this study was to evaluate the frequency of malnutrition in hospitalized patients with HF and its association with clinical outcomes in the public and private health systems. Methodology: A cross-sectional study, with 247 volunteers hospitalized with HF in three public hospitals and one private hospital in Aracaju, SE, Brazil. A subjective global nutritional assessment (SGA) and mini nutritional assessment (MNA) were performed. Results: Sample with 72.5% users of the public health system and 75.3% with malnutrition (public = 74.9%; private = 76.5%; p = 0.793). Regardless of the healthcare system, hospital stay (>14 days) was longer (p = 0.020) among those with malnutrition (48.4%) than wellnourished patients (29.5%). Malnutrition in the public system had higher mortality (7.5%; 5.8%; p < 0.001) and hospital transfer rate (21.1%; 0.0%; p < 0.001) than those in the private system. Death after discharge was observed only in the public system (p = 0.039). Conclusion: Malnutrition was frequent in both systems and was associated with longer hospital stays and, in the public hospital, in-hospital death and transfers.post-print772 K

Mind the gap of leadership : the gender mentoring program of the Building Engineering School

In order to enhance women's leadership, a Gender Mentoring Program (GMP) was implemented at the Escuela Técnica Superior de Edificación (ETSEM) from the Technical University of Madrid (UPM), during the academic year 2014-2015. This paper evaluates the GMP experience highlighting the advantages and barriers found during its implementation and giving remedial actions for the following years. The analysis carried out was mainly performed using the questionnaires answered in the sessions and the satisfaction surveys completed by all participants regarding different aspects of the GMP. Results show a high dropout rate as only 50% of the mentees completed the GMP and attended to all the sessions. 100% of them considered that the support given by the mentors was the most valued item of the Program. Moreover, 36% of mentees were very satisfied with the knowledge and the leadership experience gained, and 41% would recommend participating in the GMP. In general, the overall assessment of the Mentoring is very positive since 100% of respondents rated it above 4 (out of 5). Finally, giving ECTS credits to the mentees and reducing the duration of the program ? finishing all the training sessions in September and therefore avoiding the exams period in December?, are some of the remedial actions planned to be implemented next course in order to keep up students? participation and decrease their dropout rate

Manejo del dolor infantil por los profesionales de enfermería

Introducción: La sensación de dolor es subjetiva y en el caso de la infancia se hace más compleja su valoración debido a las dificultades que los niños tienen para comunicarse. Por tanto, se tiende a infravalorar su dolor y el tratamiento es inadecuado. Este artículo está centrado en los profesionales de Enfermería que cuidan a diario a niños que están sometidos a situaciones dolorosas por estar ingresados o padecer alguna alteración de salud. El objetivo es evaluar el grado de conocimiento de los profesionales de Enfermería sobre el dolor infantil. Metodología: Se realizó un estudio cuasiexperimental de tipo pretest-postest, en el que se distinguieron 3 Fases: I: Medida del grado de conocimiento sobre el dolor pediátrico mediante un cuestionario ya validado. II: Intervención formativa para mejorar los conocimientos, la actitud y las habilidades necesarias para el manejo adecuado del dolor. III: Evaluación de la intervención y del nivel de conocimientos tras la misma. Resultados: El resultado global más importante es que el 57% de los profesionales poseen un nivel de conocimientos considerado “Alto” y el 30% posee un nivel “Medio”. Este nivel de conocimientos es mayor en las unidades de Neonatología e inferior en las unidades de Maternidad. Conclusiones: La experiencia y la especialización suponen una cualidad para el conocimiento sobre el manejo del dolor infantil. Los profesionales necesitan más tiempo para la formación especializada y la formación mejora el conocimiento sobre el manejo del dolor infantil.Introduction: The pain sensation is subjective and in the case of the childhood it becomes more complex its valuation due to the difficulties that the children have to communicate. Therefore, he/she couches to undervalue their pain and the treatment is inadequate. This article, it is centered in the professionals of Infirmary that take care to newspaper to children that are subjected to painful situations to be entered or to suffer some alteration of health. The objective is to evaluate the grade of the professionals' of Infirmary knowledge on the infantile pain. Metodology: It is a study pretest-postest cuasiexperimental, in which is distinguished 3 Phases: I: Measured of the grade of knowledge on the pediatric pain by means of a questionnaire already validated. II: formative Intervention to improve the knowledge, the attitude and the abilities necessary for the appropriate handling of the pain. III: Evaluation of the intervention and of the level of knowledge after the same one. Results: The most important global result is that 57% of the professionals possesses a "High" considered level of knowledge and 30% it possesses a "Half" level. This level of knowledge is bigger in the units of Neonatología, and inferior in the of Maternity unidade. Conclusions: The experience and the specialization suppose a quality for the knowledge on the handling of the infantile pain. The professionals need more time for the specialized formation. and the formation improves the knowledge on the handling of the infantile pain

Los experimentos en la investigación de estrategia: una mirada múltiple

El objetivo de este artículo es mostrar la aplicabilidad de la metodología experimental en la investigación de estrategia empresarial. Para ello se delimita la metodología experimental, se destacan sus cualidades y limitaciones y se revisa su uso en el campo de la estrategia. La metodología utilizada es la revisión de literatura tanto de la caracterización de la metodología experimental como de su utilización en el campo de estrategia. Este artículo refleja la utilidad de la experimentación como metodología de investigación, destaca su complementariedad con otras metodologías e ilustra su abanico de aplicaciones en distintas temáticas. Además, se destaca su potencial de aplicación en los tópicos de investigación más recientes del campo de la estrategia

Feasibility of recycling CDW as raw material in gypsum composites

On average, Europe generates around 890 million tonnes of Construction and Demolition Waste (CDW) per year and only 50% of this CDW is recycled. This is far from the objectives determined in the European Directive for 2020 and aware of this situation, the European Countries are implementing national policies to prevent the waste that can be avoidable and to promote measures to increase recycling and recovering. In Spain, one of these measures has been the development of a CDW recycling guide for the manufacture of mortar, concrete, brick and lightweight aggregates

Plasmid conjugation from Proteobacteria as evidence for the origin of xenologous genes in Cyanobacteria

Comparative genomics showed that 5% of Synechococcus elongatus PCC 7942 genes are of probable proteobacterial origin. To investigate the role of inter-phylum conjugation in cyanobacterial gene acquisition, we tested the ability of a set of prototype proteobacterial conjugative plasmids (RP4, pKM101, R388, R64 and F) to transfer DNA from E. coli to S. elongatus. A series of BioBrick-compatible, mobilizable shuttle vectors was developed. These vectors were based on the putative origin of replication of the Synechococcus resident plasmid pANL. Not only broad-host range plasmids, such as RP4 and R388, but also narrower host-range plasmids, such as pKM101, all encoding MPFT-type IV secretion systems, were able to transfer plasmid DNA from E. coli to S. elongatus by conjugation. Neither MPFF, nor MPFI could be used as interphylum DNA delivery agents. Reciprocally, pANL-derived cointegrates could be introduced in E. coli by electroporation, where they conferred a functional phenotype. These results suggest the existence of potentially ample channels of gene flow between Proteobacteria and Cyanobacteria and point to MPFT-based inter-phylum conjugation as a potential mechanism to explain the proteobacterial origin of a majority of S. elongatus xenologous genes

Two-dimensional distributed feedback lasers with thermally-nanoimprinted perylenediimide-containing films

Two-dimensional (2D) distributed feedback (DFB) lasers with gratings imprinted by thermal nanoimprint lithography on the active film are reported. They show thresholds for lasing of ∼10 kW/cm2, similar to the most efficient imprinted DFB lasers reported; and long operational lifetimes (under ambient conditions) of ∼12 × 104 pump pulses. The key for their successful operation has been the selection of a highly efficient and stable dye, perylene orange (PDI-O), and a proper matrix to host it, the fluoro-modified thermoplastic resist mr-I7030R, which has enabled 2D imprinting while preserving the dye optical properties. The use of the UV-curable resist SU8 as an alternative matrix for PDI-O to be imprinted by combined nanoimprint and photolithography was also investigated, and was concluded to be unsuccessful due to severe photoluminescence quenching. By replacing PDI-O with Rhodamine 6G, lasers with reasonable thresholds, but with significantly inferior operational lifetimes in comparison to PDI-O/mr-I7030R devices, were obtained.We thank support from the Spanish Government (MINECO) and the European Community (FEDER) through Grants MAT2011-28167-C02 and MAT2015-66586-R, as well as to the University of Alicante. M.M-V. has been partly supported by a MINECO FPI fellowship (no. BES-2009-020747) and by a Junta de Castilla y León grant (no. SA046U16)

Distributed feedback lasers based on perylenediimide dyes for label-free refractive index sensing

The refractive index sensing capabilitity of distributed feedback (DFB) lasers based on highly photostable (under ambient conditions) perylenediimide-based active films, are reported. The sensor bulk sensitivity is determined from changes in the laser emission wavelength upon exposure to different liquids. The role of the active film thickness (hf) on the sensor sensitivity and on the laser parameters is studied. Sensors based on very thin films (hf = 160 nm) show the highest sensitivities, but their laser thresholds are relatively high and their operational durabilities moderate. The use of thicker films (hf = 850 nm) allows reducing the laser threshold and increasing the durability by two orders of magnitude. In this case, a higher sensitivity is achieved when the sensor operates at the wavelength corresponding to the first-order TE1 mode, instead of at the TE0 one. Finally, it is also shown that the inclusion of a high refractive index TiO2 layer on top of the sensor structure improves the sensitivity by around two times.This work was supported by the Spanish Government (MINECO) and the European Community (FEDER) through grant no. MAT-2011–28167-C02. This work was partially funded by the Basque Government within the framework of the Etortek Program (Grant No. IE13-360). M. Morales-Vidal has been partly supported by a MINECO FPI fellowship (no. BES-2009-020747)