Main viruses in sweet cherry plantations of Central-Western Spain

Sweet cherry trees (Prunus avium L.) are susceptible to a range of diseases, but there have been no studies to date about the viral infection of sweet cherry trees in Spain. To determine the phytosanitary status of Spanish sweet cherry plantations, the incidence and leaf symptoms induced by Prune dwarf (PDV), Prunus necrotic ringspot (PNRSV) and Apple chlorotic leaf spot (ACLSV) viruses were investigated during 2009. Young leaf samples were taken from 350 sweet cherry trees, corresponding to 17 cultivars, and were analysed by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). To associate the leaf symptoms with the virus, 50 mature leaves from each infected tree were visually inspected during the summer. The ELISA results revealed that 72 % of sweet cherry trees were infected by at least one of the viruses. PDV occurred in all sampled cultivars and presented the highest infection rate, followed by ACLSV and PNRSV. A high number of trees showed asymptomatic, in both single and mixed infections. The leaf symptoms associated with the viruses involved generalized chlorosis around the midvein (PDV), chlorotic and dark brown necrotic ringspots on both secondary veins and intervein regions (PNRSV), chlorotic and reddish necrotic ringspots (ACLSV) and generalized interveinal chlorosis (PDV-PNRSV)

Sour and duke cherry viruses in South-West Europe

This study investigated the phytosanitary status of sour and duke cherry genetic resources in the Iberian Peninsula, and the incidence and leaf symptoms induced by the Prunus necrotic ringspot virus (PNRSV), Prune dwarf virus (PDV) and Apple chlorotic leaf spot virus (ACLSV). Young leaf samples were taken from 204 sour and duke cherry trees belonging to ten cultivars, and were assayed by DAS-ELISA. Samples positive for any of the three viruses were also tested by RT-PCR. To associate the leaf symptoms with virus presence, 50 mature leaves from each infected tree were visually inspected during the summer. The ELISA and RT-PCR results indicated that 63% of the cherry trees were infected by at least one of these viruses. PNRSV occurred in all cultivars sampled and presented the highest infection rate (46%), followed by PDV (31%) and ACLSV (6%). Many trees, (60 to 100%), were asymptomatic while harbouring single and mixed virus infections. The leaf symptoms associated with the viruses included chlorotic and dark brown necrotic ringspots on secondary veins and interveinal regions, for PNRSV, generalized chlorosis around the midveins, for PDV, chlorotic and reddish necrotic ringspots, for ACLSV, and generalized interveinal chlorosis, for mixed PNRSV and PDVinfections

Predicting the physicochemical properties and geographical ORIGIN of lentils using near infrared spectroscopy

[EN]Calibration statistical descriptors for both whole and ground lentils using Near Infrared Spectroscopy (NIRS), combined with fiber-optic probe, are presented and discussed. The models were developed for estimating the weight, size, total raw protein, moisture, total fat, total fiber, and ash. Standard methods were used to determine compositional parameters of 42 samples of different varieties of lentils. The calibration curves show a wide range of validity for all parameters. The results showed excellent predictability for the determination of weight, fiber, and ash in whole lentils. However, size, moisture, and total fat were predicted satisfactorily in ground lentils. The total protein content could be predicted for both whole and ground lentils. Moreover, NIRS and Direct Partial Least Squares (DPLS) were used to determine whether a sample of lentils belonged to the Protected Geographical Indication (PGI) “Lenteja de La Armuña” or not. The results showed that 95% of the samples were correctly classified as belonging to a PGI. This result demonstrates that this technique allows the differentiation of samples from nearby regions

¿Puede considerarse diferente la calidad de la proteína y grasa de la leche de Cabra y vaca?

Peer reviewe

Elaboración de material didáctico para actividades prácticas: casos prácticos de sanidad vegetal (3º grado de Ingeniería Agrícola)

Memoria ID-085. Ayudas de la Universidad de Salamanca para la innovación docente, curso 2013-2014

Elaboración de material didáctico para actividades prácticas. Casos prácticos de sanidad vegetal (3º Grado de Ingeniería Agrícola). 2ª edición

Memoria ID-0147. Ayudas de la Universidad de Salamanca para la innovación docente, curso 2014-2015

Instalación de un laboratorio de prácticas en la facultad de ciencias agrarias y ambientales (Universidad de Salamanca)

Memoria ID12-0104. Ayudas de la Universidad de Salamanca para la innovación docente, curso 2012-2013