The Gun

Scholarships & Prizes Office. University of Sydne

Chesnut-Miller-Manning Papers - Accession 771

The Chesnut-Miller-Manning Family Papers consist of financial and property records, correspondence, estate records, legal documents, and other items. Included are the papers of John Chesnut (1743-1818), James Chesnut, Sr. (1773-1866), James Chesnut, Jr. (1815-1885), Stephen Decatur Miller (1787-1838), and John Laurence Manning (1816-1889). Papers pertain to the business, financial, and plantation affairs and political activities of three generations of the Chesnut family, John Chesnut, James Chesnut, Sr. and James Chesnut, Jr., as well as James Chesnut, Jr.’s father-in-law Stephen Decatur Miller and John Laurence Manning.https://digitalcommons.winthrop.edu/manuscriptcollection_findingaids/1760/thumbnail.jp

A Preliminary List of Some Families of Iowa Insects

For some ten years the Iowa Insect Survey has been collecting specimens and data from all parts of this state in a study of the geographic and seasonal distribution of the insects of Iowa. Comparatively little has been done heretofore with most of the families of the Hymenoptera and the Diptera of Iowa. With the hope of stimulating a more general interest in these groups, preliminary lists of the species now known to occur within the state are being submitted. Students in systematic Entomology in the college have taken a family and given it special attention in their collecting for one or more years. These students have collaborated with the senior author in the preparation of this paper. The name of the student thus taking the responsibility of getting a list for a family in shape is printed at the head of the list. Determinations throughout the list have been made or checked by specialists as indicated for each group. Assistance given by the Iowa Academy of Science and the State University of Iowa has helped materially in keeping the Survey going

MoKCa database - mutations of kinases in cancer

Members of the protein kinase family are amongst the most commonly mutated genes in human cancer, and both mutated and activated protein kinases have proved to be tractable targets for the development of new anticancer therapies The MoKCa database (Mutations of Kinases in Cancer, http://strubiol.icr.ac.uk/extra/mokca) has been developed to structurally and functionally annotate, and where possible predict, the phenotypic consequences of mutations in protein kinases implicated in cancer. Somatic mutation data from tumours and tumour cell lines have been mapped onto the crystal structures of the affected protein domains. Positions of the mutated amino-acids are highlighted on a sequence-based domain pictogram, as well as a 3D-image of the protein structure, and in a molecular graphics package, integrated for interactive viewing. The data associated with each mutation is presented in the Web interface, along with expert annotation of the detailed molecular functional implications of the mutation. Proteins are linked to functional annotation resources and are annotated with structural and functional features such as domains and phosphorylation sites. MoKCa aims to provide assessments available from multiple sources and algorithms for each potential cancer-associated mutation, and present these together in a consistent and coherent fashion to facilitate authoritative annotation by cancer biologists and structural biologists, directly involved in the generation and analysis of new mutational data

Porcupine Bank Nephrops Grounds (FU16) 2023 UWTV Survey Report and catch scenarios for 2024

This report provides the results of the eleventh underwater television on the ‘Porcupine Bank Nephrops grounds’ ICES assessment area; Functional Unit 16. The survey was multi disciplinary in nature collecting UWTV and other ecosystem data. In total 71 UWTV stations were successfully completed (100% of the planned stations) in a randomised 6 nautical mile isometric grid covering the full spatial extent of the stock. The mean burrow density observed in 2023, adjusted for edge effect, was 0.27 burrows/m². The final krigged abundance estimate was 2002 million burrows with a CV of 3% and an estimated stock area of 7,130 km2 . The 2023 abundance estimate was 47% higher than in 2022. Using the 2023 estimate of abundance and updated stock data imply that catches in 2024 should be between 3677 and 4560 tonnes, according to the EU MAP and ICES MSY approach (assuming that all catch is landed). Four species of sea-pen (Virgularia mirabilis, Funiculina quadrangularis, Pennatula phosphorea and the deepwater sea-pen Kophobelemnon stelliferum) were observed during the survey. Trawl marks were also observed on 20% of the stations surveyed.Marine Institut

Visual motion and decision-making in dyslexia: reduced accumulation of sensory evidence and related neural dynamics

Children with and without dyslexia differ in their behavioural responses to visual information, particularly when required to pool dynamic signals over space and time. Importantly, multiple processes contribute to behavioural responses. Here we investigated which processing stages are affected in children with dyslexia when performing visual motion processing tasks, by combining two methods that are sensitive to the dynamic processes leading to responses. We used a diffusion model which decomposes response time and accuracy into distinct cognitive constructs, and high-density EEG. 50 children with dyslexia (24 male) and 50 typically developing children (28 male) aged 6 to 14 years judged the direction of motion as quickly and accurately as possible in two global motion tasks (motion coherence and direction integration), which varied in their requirements for noise exclusion. Following our pre-registered analyses, we fitted hierarchical Bayesian diffusion models to the data, blinded to group membership. Unblinding revealed reduced evidence accumulation in children with dyslexia compared to typical children for both tasks. Additionally, we identified a response-locked EEG component which was maximal over centro-parietal electrodes which indicated a neural correlate of reduced drift-rate in dyslexia in the motion coherence task, thereby linking brain and behaviour. We suggest that children with dyslexia tend to be slower to extract sensory evidence from global motion displays, regardless of whether noise exclusion is required, thus furthering our understanding of atypical perceptual decision-making processes in dyslexia

Motor-dependent microtubule disassembly driven by tubulin tyrosination

In cells, stable microtubules (MTs) are covalently modified by a carboxypeptidase, which removes the C-terminal Tyr residue of α-tubulin. The significance of this selective detyrosination of MTs is not understood. In this study, we report that tubulin detyrosination in fibroblasts inhibits MT disassembly. This inhibition is relieved by overexpression of the depolymerizing motor mitotic centromere-associated kinesin (MCAK). Conversely, suppression of MCAK expression prevents disassembly of normal tyrosinated MTs in fibroblasts. Detyrosination of MTs suppresses the activity of MCAK in vitro, apparently as the result of a decreased affinity of the adenosine diphosphate (ADP)–inorganic phosphate- and ADP-bound forms of MCAK for the MT lattice. Detyrosination also impairs MT disassembly in neurons and inhibits the activity of the neuronal depolymerizing motor KIF2A in vitro. These results indicate that MT depolymerizing motors are directly inhibited by the detyrosination of tubulin, resulting in the stabilization of cellular MTs. Detyrosination of transiently stabilized MTs may give rise to persistent subpopulations of disassembly-resistant polymers to sustain subcellular cytoskeletal differentiation

One class classification as a practical approach for accelerating π–π co-crystal discovery

Machine learning using one class classification on a database of existing co-crystals enables the identification of co-formers which are likely to form stable co-crystals, resulting in the synthesis of two co-crystals of polyaromatic hydrocarbons.</p

Targeted long-read sequencing of the Ewing sarcoma 6p25.1 susceptibility locus identifies germline-somatic interactions with EWSR1-FLI1 binding

Ewing sarcoma (EwS) is a rare bone and soft tissue malignancy driven by chromosomal translocations encoding chimeric transcription factors, such as EWSR1-FLI1, that bind GGAA motifs forming novel enhancers that alter nearby expression. We propose that germline microsatellite variation at the 6p25.1 EwS susceptibility locus could impact downstream gene expression and EwS biology. We performed targeted long-read sequencing of EwS blood DNA to characterize variation and genomic features important for EWSR1-FLI1 binding. We identified 50 microsatellite alleles at 6p25.1 and observed that EwS-affected individuals had longer alleles (>135 bp) with more GGAA repeats. The 6p25.1 GGAA microsatellite showed chromatin features of an EWSR1-FLI1 enhancer and regulated expression of RREB1, a transcription factor associated with RAS/MAPK signaling. RREB1 knockdown reduced proliferation and clonogenic potential and reduced expression of cell cycle and DNA replication genes. Our integrative analysis at 6p25.1 details increased binding of longer GGAA microsatellite alleles with acquired EWSR-FLI1 to promote Ewing sarcomagenesis by RREB1-mediated proliferation

Treatment as Prevention: Characterization of Partner Infections in the HIV Prevention Trials Network 052 Trial

HIV Prevention Trials Network (HPTN) 052 demonstrated that antiretroviral therapy (ART) prevents HIV transmission in serodiscordant couples. HIV from index-partner pairs was analyzed to determine the genetic linkage status of partner infections. Forty-six infections were classified as linked, indicating that the index was the likely source of the partner’s infection. Lack of viral suppression and higher index viral load were associated with linked infection. Eight linked infections were diagnosed after the index started ART: four near the time of ART initiation and four after ART failure. Linked infections were not observed when the index participant was stably suppressed on ART