Light-induced rhythmic changes in thermotolerance in stationary-phase cells of Candida utilis

The original publication is available on LINK at In synchronized light-dark cycles, stationary-phase cultures of the budding yeast Candida utilis were able to survive heat treatment at 50ºC with an apparent circadian-like rhythm related to the onset of light. However, in continuous darkness this pattern did not run freely and was markedly dampened. We discuss these findings in terms of the potential circadian control of heat tolerance, which has been described in the fission yeast Schizosaccharomyces pombe. Our results suggest that the resistance pattern observed in C. utilis is most likely an adaptive response to the light-induced generation of reactive oxygen species rather than the occurrence of a truly endogenous circadian rhythmLa supervivencia de cultivos de Candida utilisen la fase estacionaria tras ser sometidos a temperaturas de 50ºC en ciclos sincronizados de luz/oscuridad presentó un aparente ritmo circadiano relacionado con el inicio de la fase iluminada. Sin embargo, en condiciones de oscuridad continua este patrón no se observaba tan claramente y mostraba una marcada ambigüedad. Estas observaciones se discuten en términos de un posible control circadiano de la tolerancia a altas temperaturas, que ha sido descrito para la levadura Schizosaccharomyces pombe. Nuestros resultados indican que el patrón de resistencia observado en C. utilises muy probablemente una respuesta adaptativa a la generación de especies reactivas de oxígeno inducida por luz y que no existe un verdadero ritmo circadiano endógeno.Asobrevivência de cultivos de Candida utilis na fase estacionária depois de ser submetidos a temperaturas de 50ºC em ciclos sincronizados de luz/escuridão apresentou um aparente ritmo circadiano relacionado com o início da fase iluminada. No entanto, em condições de escuridão contínua este patrão não foi observado tão claramente e mostrou uma marcada ambigüidade. Estas observações são discutidas com relação a um possível controle circadiano da tolerância a altas temperaturas, que foi descrito para a levedura Schizosaccharomyces pombe. Nossos resultados indicam que o patrão de resistência observado em C. utilisé muito provavelmente uma resposta adaptativa à geração de espécies reativas de oxigênio induzida por luz e que não existe um verdadeiro ritmo circadiano endógeno

Light-induced rhythmic changes in thermotolerance in stationary-phase cells of Candida utilis

In synchronized light-dark cycles, stationary-phase cultures of the budding yeast Candida utilis were able to survive heat treatment at 50ºC with an apparent circadian-like rhythm related to the onset of light. However, in continuous darkness this pattern did not run freely and was markedly dampened. We discuss these findings in terms of the potential circadian control of heat tolerance, which has been described in the fission yeast Schizosaccharomyces pombe. Our results suggest that the resistance pattern observed in C. utilis is most likely an adaptive response to the light-induced generation of reactive oxygen species rather than the occurrence of a truly endogenous circadian rhythm. [Int Microbiol 2006; 9(1):61-64

Orientatio ad sidera: Astronomy and landscape in Qart Hadašt/Carthago Nova

[ES] Las investigaciones arqueológicas en Cartagena –la antigua Qart Hadašt púnica, posterior Carthago Nova romana– evidencian la existencia de elementos topográficos, urbanísticos y rituales, susceptibles de ser analizados desde la perspectiva de la Astronomía Cultural. Por ello, en octubre de 2013 un equipo interdisciplinar de astrofísicos y arqueólogos realizó una campaña de mediciones de los principales hitos topográficos y arqueológicos de la ciudad púnica y romana. Metodológicamente, para cada ítem se estableció el criterio básico de orientación y se tomó su azimut utilizando tres tándems de brújula de precisión más clinómetro. Los datos obtenidos demuestran la relevancia en la ciudad antigua de una serie de orientaciones hacia la salida y la puesta del sol en el solsticio de verano, cuya significación se integra plenamente en el contexto del ritual fenicio-púnico. Dicho sentido pudo incorporarse y reinterpretarse en el contexto de las posteriores refundaciones romanas y de sus sucesivos programas urbanísticos y arquitectónicos, en particular el del período augústeo, cuando determinadas orientaciones astronómicas pudieron servir para afianzar la imagen de Roma y el princeps como restauradores de la paz y garantes de un nuevo orden fundado en elementos cosmológicos.[EN] Archaeological investigations in Cartagena -the ancient Punic Qart Hadašt, Roman Carthago Nova- have manifested the existence of ritual, urban and topographical elements that could be analyzed from the perspective of Cultural Astronomy. Therefore, in October 2013, an interdisciplinary team of astronomers and archaeologists conducted a field campaign of the main topographic and archaeological landmarks of the Punic and Roman periods of the city. Methodologically, a basic guide criterion was established for each particular element, measuring its corresponding azimuth(s). Three tandems, including precision compasses and clinometers, were used to take the measurements. The data obtained have demonstrated the relevance, within the ancient city, of a series of orientations towards sunrise and sunset at the summer solstice, whose significance could be fully integrated within the context of the Punic ritual. This skyscaping was merged and reinterpreted in the framework of the subsequent Roman appropriation of the city landscape, including their successive urban and architectural programs, in particular that of the period of Emperor Augustus, when certain astronomical orientations could serve to strengthen the image of Rome and the 'Princeps' as restorers of peace and guarantees of a new order based in cosmological elements.Peer Reviewe

Collaborative working: understanding mobile applications requirements

1 archivo PDF (4 páginas)The Rational Unified Process (RUP) as well as the related work embrace the importance of collaborative working teams during software development; but, user-interface designers and system analysts work in parallel or in sequential mode. However, this kind of relationship may not be effective, resulting on functional software but not meeting usability issues. Our proposal is that in order to understand mobile applications requirements work should be made collaboratively between analysts and user-interface designers through sharing artifacts like use-case scenarios, sketching and mock-up. In this paper, we propose a collaborative work framework to meet mobile applications requirements. Also, we show preliminary results of a case study to assess this approach. Results suggest that the collaborative team got a common understanding about system limits and functional and usability requirements.Universidad Autónoma Metropolitan

Transportability of non-target arthropod field data for the use in environmental risk assessment of genetically modified maize in Northern Mexico

In country, non-target arthropod (NTA) field evaluations are required to comply with the regulatory process for cultivation of genetically modified (GM) maize in Mexico. Two sets of field trials, Experimental Phase and Pilot Phase, were conducted to identify any potential harm of insect-protected and glyphosate-tolerant maize (MON89Ø34-3 × MON-88Ø17-3 and MON-89Ø34-3 × MON-ØØ6Ø3-6) and glyphosatetolerant maize (MON-ØØ6Ø3-6) to local NTAs compared to conventional maize. NTA abundance data were collected at 32 sites, providing high geographic and environmental diversity within maize production areas from four ecological regions (ecoregions) in northern Mexico. The most abundant herbivorous taxa collected included field crickets, corn flea beetles, rootworm beetles, cornsilk flies, aphids, leafhoppers, plant bugs and thrips while the most abundant beneficial taxa captured were soil mites, spiders, predatory ground beetles, rove beetles, springtails (Collembola), predatory earwigs, ladybird beetles, syrphid flies, tachinid flies, minute pirate bugs, parasitic wasps and lacewings. Across the taxa analysed, no statistically significant differences in abundance were detected between GM maize and the conventional maize control for 69 of the 74 comparisons (93.2%) indicating thatthe single or stacked insect-protected and herbicide-tolerant GM traits generally exert no marked adverse effects on the arthropod populations compared with conventional maize. The distribution of taxa observed in this study provides evidence that irrespective of variations in overall biodiversity of a given ecoregion, important herbivore, predatory and parasitic arthropod taxa within the commercial maize agroecosystem are highly similar indicating that relevant data generated in one ecoregion can be transportable for the risk assessment of the same or similar GM crop in another ecoregion

Measurement procedures for an adaptive antenna system: application to WCDMA

Adaptive type smart antennas have not been implemented yet on the deployed UMTS systems, although UTRA-UMTS preview their operation and they also could improve capacity especially in a multiservice environment. This paper describes a set of novel measurement techniques that must be performed to evaluate the correct operation of a smart antenna system. The paper also describes the measurements carried out on a UMTS smart antenna prototype

Nephrin mutations cause childhood- and adult-onset focal segmental glomerulosclerosis

Mutations in the NPHS1 gene cause congenital nephrotic syndrome of the Finnish type presenting before the first 3 months of life. Recently, NPHS1 mutations have also been identified in childhood-onset steroid-resistant nephrotic syndrome and milder courses of disease, but their role in adults with focal segmental glomerulosclerosis remains unknown. Here we developed an in silico scoring matrix to evaluate the pathogenicity of amino-acid substitutions using the biophysical and biochemical difference between wild-type and mutant amino acid, the evolutionary conservation of the amino-acid residue in orthologs, and defined domains, with the addition of contextual information. Mutation analysis was performed in 97 patients from 89 unrelated families, of which 52 presented with steroid-resistant nephrotic syndrome after 18 years of age. Compound heterozygous or homozygous NPHS1 mutations were identified in five familial and seven sporadic cases, including one patient 27 years old at onset of the disease. Substitutions were classified as ‘severe’ or ‘mild’ using this in silico approach. Our results suggest an earlier onset of the disease in patients with two ‘severe’ mutations compared to patients with at least one ‘mild’ mutation. The finding of mutations in a patient with adult-onset focal segmental glomerulosclerosis indicates that NPHS1 analysis could be considered in patients with later onset of the disease

Posible efecto de los canabinoides endógenos y el rimonabant sobre la insulinorresistencia y la función de las células β

El propósito del trabajo es estudiar el posible papel regulador del sistema canabinoide sobre la secreción de insulina y los cambios endocrino-metabólicos inducidos en ratas normales por una dieta rica en fructosa (DRF).Facultad de Ciencias Médicas (FCM

COSMO: Corrientes Marinas y Seguridad en el Medio Marino

Ejercicio internacional de salvamento y lucha contra la contaminación marina "Polex 24-17" organizado por la Dirección General de Marina Mercante y Salvamento Marítimo del 14 al 16 de junio de 2017 en SantanderDisponer en tiempo real de información sobre las corrientes oceánicas es clave para algunos de los servicios encomendados a la Sociedad de Salvamento y Seguridad Marítima y al Cuerpo Nacional de Policía (CNP). Un alto porcentaje de las emergencias de búsqueda de personas y náufragos, y de los incidentes de contaminación gestionados por Salvamento Marítimo, tienen lugar en zonas cercanas a la costa. Asimismo, el 71% de los casos de restos humanos no identificados (CSI) acontecidos en España durante el período 1968-2015 se da en zonas costeras. El proyecto COSMO busca mejorar la eficacia de las operaciones de búsqueda y de predicción de derivas, y mejorar la proporción de identificaciones positivas acelerando la resolución de casos de recuperación de restos humanosProyecto cofinanciado por el Ministerio de Economía Industria y Competitividad y Fondos FEDER de la UE (COSMO-CTM2016-79474-R, UE)Peer Reviewe

The Expanded mtDNA Phylogeny of the Franco-Cantabrian Region Upholds the Pre-Neolithic Genetic Substrate of Basques

The European genetic landscape has been shaped by several human migrations occurred since Paleolithic times. The accumulation of archaeological records and the concordance of different lines of genetic evidence during the last two decades have triggered an interesting debate concerning the role of ancient settlers from the Franco-Cantabrian region in the postglacial resettlement of Europe. Among the Franco-Cantabrian populations, Basques are regarded as one of the oldest and more intriguing human groups of Europe. Recent data on complete mitochondrial DNA genomes focused on macrohaplogroup R0 revealed that Basques harbor some autochthonous lineages, suggesting a genetic continuity since pre-Neolithic times. However, excluding haplogroup H, the most representative lineage of macrohaplogroup R0, the majority of maternal lineages of this area remains virtually unexplored, so that further refinement of the mtDNA phylogeny based on analyses at the highest level of resolution is crucial for a better understanding of the European prehistory. We thus explored the maternal ancestry of 548 autochthonous individuals from various Franco-Cantabrian populations and sequenced 76 mitogenomes of the most representative lineages. Interestingly, we identified three mtDNA haplogroups, U5b1f, J1c5c1 and V22, that proved to be representative of Franco-Cantabria, notably of the Basque population. The seclusion and diversity of these female genetic lineages support a local origin in the Franco-Cantabrian area during the Mesolithic of southwestern Europe, ∼10,000 years before present (YBP), with signals of expansions at ∼3,500 YBP. These findings provide robust evidence of a partial genetic continuity between contemporary autochthonous populations from the Franco-Cantabrian region, specifically the Basques, and Paleolithic/Mesolithic hunter-gatherer groups. Furthermore, our results raise the current proportion (≈15%) of the Franco-Cantabrian maternal gene pool with a putative pre-Neolithic origin to ≈35%, further supporting the notion of a predominant Paleolithic genetic substrate in extant European populations