Confocal laser scanning microscope, raman microscopy and western blotting to evaluate inflammatory response after myocardial infarction

Cardiac muscle necrosis is associated with inflammatory cascade that clears the infarct from dead cells and matrix debris, and then replaces the damaged tissue with scar, through three overlapping phases: the inflammatory phase, the proliferative phase and the maturation phase. Western blotting, laser confocal microscopy, Raman microscopy are valuable tools for studying the inflammatory response following myocardial infarction both humoral and cellular phase, allowing the identification and semiquantitative analysis of proteins produced during the inflammatory cascade activation and the topographical distribution and expression of proteins and cells involved in myocardial inflammation. Confocal laser scanning microscopy (CLSM) is a relatively new technique for microscopic imaging, that allows greater resolution, optical sectioning of the sample and three-dimensional reconstruction of the same sample. Western blotting used to detect the presence of a specific protein with antibody-antigen interaction in the midst of a complex protein mixture extracted from cells, produced semi-quantitative data quite easy to interpret. Confocal Raman microscopy combines the three-dimensional optical resolution of confocal microscopy and the sensitivity to molecular vibrations, which characterizes Raman spectroscopy. The combined use of western blotting and confocal microscope allows detecting the presence of proteins in the sample and trying to observe the exact location within the tissue, or the topographical distribution of the same. Once demonstrated the presence of proteins (cytokines, chemokines, etc.) is important to know the topographical distribution, obtaining in this way additional information regarding the extension of the inflammatory process in function of the time stayed from the time of myocardial infarction. These methods may be useful to study and define the expression of a wide range of inflammatory mediators at several different timepoints providing a more detailed analysis of the time course of the infarct

Persistent and radiation-induced currents in distorted quantum rings

Persistent and radiation-induced currents in distorted narrow quantum rings are theoretically investigated. We show that ring distorsions can be described using a geometrical potential term. We analyse the effect of this term on the current induced by a magnetic flux (persistent current) and by a polarized coherent electromagnetic field (radiation-induced current). The strongest effects in persistent currents are observed for distorted rings with a small number of electrons. The distortion smoothes the current oscillations as a function of the magnetic flux and changes the temperature dependence of the current amplitude. For radiation-induced currents, the distortion induces an ac component in the current and affects its dependence on the radiation frequency and intensity

Short-Baseline Electron Neutrino Disappearance, Tritium Beta Decay and Neutrinoless Double-Beta Decay

We consider the interpretation of the MiniBooNE low-energy anomaly and the Gallium radioactive source experiments anomaly in terms of short-baseline electron neutrino disappearance in the framework of 3+1 four-neutrino mixing schemes. The separate fits of MiniBooNE and Gallium data are highly compatible, with close best-fit values of the effective oscillation parameters Delta m^2 and sin^2 2 theta. The combined fit gives Delta m^2 >~ 0.1 eV^2 and 0.11 < sin^2 2 theta < 0.48 at 2 sigma. We consider also the data of the Bugey and Chooz reactor antineutrino oscillation experiments and the limits on the effective electron antineutrino mass in beta-decay obtained in the Mainz and Troitsk Tritium experiments. The fit of the data of these experiments limits the value of sin^2 2 theta below 0.10 at 2 sigma. Considering the tension between the neutrino MiniBooNE and Gallium data and the antineutrino reactor and Tritium data as a statistical fluctuation, we perform a combined fit which gives Delta m^2 \simeq 2 eV and 0.01 < sin^2 2 theta < 0.13 at 2 sigma. Assuming a hierarchy of masses m_1, m_2, m_3 << m_4, the predicted contributions of m_4 to the effective neutrino masses in beta-decay and neutrinoless double-beta-decay are, respectively, between about 0.06 and 0.49 and between about 0.003 and 0.07 eV at 2 sigma. We also consider the possibility of reconciling the tension between the neutrino MiniBooNE and Gallium data and the antineutrino reactor and Tritium data with different mixings in the neutrino and antineutrino sectors. We find a 2.6 sigma indication of a mixing angle asymmetry.Comment: 14 pages; final version published in Phys.Rev.D82:053005,201

Single muscle fiber proteomics reveals unexpected mitochondrial specialization

Mammalian skeletal muscles are composed of multinucleated cells termed slow or fast fibers according to their contractile and metabolic properties. Here, we developed a high-sensitivity workflow to characterize the proteome of single fibers. Analysis of segments of the same fiber by traditional and unbiased proteomics methods yielded the same subtype assignment. We discovered novel subtype-specific features, most prominently mitochondrial specialization of fiber types in substrate utilization. The fiber type-resolved proteomes can be applied to a variety of physiological and pathological conditions and illustrate the utility of single cell type analysis for dissecting proteomic heterogeneity

On the environmental stability of quantum chaotic ratchets

The transitory and stationary behavior of a quantum chaotic ratchet consisting of a biharmonic potential under the effect of different drivings in contact with a thermal environment is studied. For weak forcing and finite $\hbar$, we identify a strong dependence of the current on the structure of the chaotic region. Moreover, we have determined the robustness of the current against thermal fluctuations in the very weak coupling regime. In the case of strong forcing, the current is determined by the shape of a chaotic attractor. In both cases the temperature quickly stabilizes the ratchet, but in the latter it also destroys the asymmetry responsible for the current generation. Finally, applications to isomerization reactions are discussed.Comment: 6 pages, 5 figure

Petrography, mineralogy and geochemistry of a primitive pumice from Stromboli: implications for the deep feeding system

We describe the field relations, petrographic, mineralogical and geochemical characteristics of an exceptional “golden” pumice belonging to a tephra layer exposed on the summit area of Stromboli volcano, Italy. Pumice sample PST-9 comes from a fallout deposit older than a spatter agglutinate sequence emplaced during the twentieth century. The eruption that produced it had a size exceeding that of intermediate paroxysms but was smaller than large-scale, spatter-forming, paroxysms from the sixteenth century and 1930 A.D. Lapilli are strongly vesicular and crystal-poor, similar to other “golden” pumices. Modal proportions include 89 vol% glass, 8 vol% clinopyroxene, 1–2 vol% olivine and 1–2 vol% plagioclase. Plagioclase is represented by reacted crystals coming from the shallow resident magma and incorporated in the pumice during eruption. A total of 74 and 44 crystals of olivine and clinopyroxene, respectively, were examined and 187 and 99 electron microprobe analyses obtained. Fo in olivine ranges between 70 and 92 mol% and Fs in clinopyroxene between 3 and 13 mol%. PST-9 hosts a higher proportion of Fo-rich olivine and Fs-poor clinopyroxene than the other “golden” pumices. Groundmass glasses are basaltic (Mg# = 66–69), as are most rim glasses around olivine and clinopyroxene, and glass inclusions in clinopyroxene. They are more primitive than in the other “golden” pumices. A few rim glasses and glass inclusions are shoshonitic (Mg# = 45–50). Most glass inclusions in olivine have CaO/Al2O3 higher than the other glasses and the whole-rock. PST-9 has the highest bulk MgO, CaO, Mg# and CaO/Al2O3 and the lowest FeOt of all “golden” pumices analysed to date. Analysis of Fe-Mg partitioning between olivine, clinopyroxene and melt allows three crystallization stages to be recognized. The first involves primitive mantle-derived melts (Mg# = 74–80), the second basaltic melts represented by groundmass glasses and the third is associated with more evolved melts represented by the shoshonitic glasses. The population of crystals in “golden” pumices is heterogeneous not only because of crystal incorporation from the shallow resident magma, but also because of pre-eruptive recharge of the deep reservoir with primitive melts. Differences between PST-9 and the other “golden” pumices in terms of groundmass glass composition and distribution of olivine and clinopyroxene compositions reflect contrasted replenishment rates of the deep reservoir with primitive liquids. Gabbroic inclusions in a clinopyroxene crystal provide a direct illustration of melt wall-rock interaction and stress the variability of the deep reservoir in terms of temperature, crystallinity and phase assemblages. Deep crystallization of plagioclase should be considered as a possibility at Stromboli. PST-9 is exceptionally well representative of the early magmatic evolution of “golden” pumices

Strong disorder fixed point in absorbing state phase transitions

The effect of quenched disorder on non-equilibrium phase transitions in the directed percolation universality class is studied by a strong disorder renormalization group approach and by density matrix renormalization group calculations. We show that for sufficiently strong disorder the critical behaviour is controlled by a strong disorder fixed point and in one dimension the critical exponents are conjectured to be exact: \beta=(3-\sqrt{5})/2 and \nu_\perp=2. For disorder strengths outside the attractive region of this fixed point, disorder dependent critical exponents are detected. Existing numerical results in two dimensions can be interpreted within a similar scenario.Comment: final version as accepted for PRL, contains new results in two dimension

Heat Sensing Receptor TRPV1 Is a Mediator of Thermotaxis in Human Spermatozoa

The molecular bases of sperm thermotaxis, the temperature-oriented cell motility, are currently under investigation. Thermal perception relies on a subclass of the transient receptor potential [TRP] channels, whose member TRPV1 is acknowledged as the heat sensing receptor. Here we investigated the involvement of TRPV1 in human sperm thermotaxis. We obtained semen samples from 16 normozoospermic subjects attending an infertility survey programme, testis biopsies from 6 patients with testicular germ cell cancer and testis fine needle aspirates from 6 patients with obstructive azoospermia undergoing assisted reproductive technologies. Expression of TRPV1 mRNA was assessed by RT-PCR. Protein expression of TRPV1 was determined by western blot, flow cytometry and immunofluorescence. Sperm motility was assessed by Sperm Class Analyser. Acrosome reaction, apoptosis and intracellular-Ca2+ content were assessed by flow cytometry. We found that TRPV1 mRNA and protein were highly expressed in the testis, in both Sertoli cells and germ-line cells. Moreover, compared to no-gradient controls at 31°C or 37°C (Ctrl 31°C and Ctrl 37°C respectively), sperm migration towards a temperature gradient of 31-37°C (T gradient) in non-capacitated conditions selected a higher number of cells (14,9 ± 4,2×106 cells T gradient vs 5,1± 0,3×106 cells Ctrl 31°C and 5,71±0,74×106 cells Ctrl 37°C; P = 0,039). Capacitation amplified the migrating capability towards the T gradient. Sperms migrated towards the T gradient showed enriched levels of both TRPV1 protein and mRNA. In addition, sperm cells were able to migrate toward a gradient of capsaicin, a specific agonist of TRPV1, whilst capsazepine, a specific agonist of TRPV1, blocked this effect. Finally, capsazepine severely blunted migration towards T gradient without abolishing. These results suggest that TRPV1 may represent a facilitating mediator of sperm thermotaxis