38 research outputs found
Individualism, collectivism, and dissonance: a within-culture comparison
Cognitive dissonance was examined among individuals with collectivist and individualist tendencies within a culture. To arouse dissonance, participants wrote counter-attitudinal essays under either high-choice or low-choice conditions. Participants were also given an individualism-collectivism questionnaire. Results indicated that participants in the high-choice condition experienced more cognitive dissonance (measured by degree of attitude change) than participants in the low-choice condition. Participants who scored high in individualism showed no significant difference in dissonance experienced compared to those who scored low in individualism. These results suggest that differences in cognitive dissonance experienced cross-culturally are due to factors other than individualism. Possible alternative explanations for the absence of a relationship between individualism and dissonance are discussed
Structure of the sporulation histidine kinase inhibitor Sda from Bacillus subtilis and insights into its solution state
The crystal structure of the DNA-damage checkpoint inhibitor of sporulation, Sda, from Bacillus subtilis, has been solved by the MAD technique using selenomethionine-substituted protein. The structure closely resembles that previously solved by NMR, as well as the structure of a homologue from Geobacillus stearothermophilus solved in complex with the histidine kinase KinB. The structure contains three molecules in the asymmetric unit. The unusual trimeric arrangement, which lacks simple internal symmetry, appears to be preserved in solution based on an essentially ideal fit to previously acquired scattering data for Sda in solution. This interpretation contradicts previous findings that Sda was monomeric or dimeric in solution. This study demonstrates the difficulties that can be associated with the characterization of small proteins and the value of combining multiple biophysical techniques. It also emphasizes the importance of understanding the physical principles behind these techniques and therefore their limitations
Lovers in a dangerous time: Ecologically motivated relationship safety regulation
The proposed model of ecologically motivated relationship safety regulation posits that close relationships offer symbolic safety when natural ecologically-based threats activate the need for self-preservation. The model makes the twin assumptions that (1) natural ecological threats motivate people in unreliable relationships to perceive their relationships as bastions of safety, but (2) that their personal capacities for resilience in the face of threat constrain such motivated perceptions. Two internal meta-analyses of 4 correlational/cross-sectional and 5 experimental studies (Ntotal=5,030) using different methods and conceptualizations of natural ecological threats (acute and chronic pain; pathogenic transmission) supported the hypotheses. People in less satisfying relationships symbolically defended against natural ecological threats by affirming the available safety in their close relationships when they were high in self-esteem (i.e., high in personal resiliency), but not when they were low in self-esteem. However, people in highly satisfying relationships did not defend against natural ecological threats, likely because they already felt safe in their relationships
Tidally trapped pulsations in a close binary star system discovered by TESS
It has long been suspected that tidal forces in close binary stars could modify the orientation of the pulsation axis of the constituent stars. Such stars have been searched for, but until now never detected. Here we report the discovery of tidally trapped pulsations in the ellipsoidal variable HD 74423 in Transiting Exoplanet Survey Satellite (TESS) space photometry data. The system contains a δ Scuti pulsator in a 1.6 d orbit, whose pulsation mode amplitude is strongly modulated at the orbital frequency, which can be explained if the pulsations have a much larger amplitude in one hemisphere of the star. We interpret this as an obliquely pulsating distorted dipole oscillation with a pulsation axis aligned with the tidal axis. This is the first time that oblique pulsation along a tidal axis has been recognized. It is unclear whether the pulsations are trapped in the hemisphere directed towards the companion or in the side facing away from it, but future spectral measurements can provide the solution. In the meantime, the single-sided pulsator HD 74423 stands out as the prototype of a new class of obliquely pulsating stars in which the interactions of stellar pulsations and tidal distortion can be studied
A Novel Biochemical Route for Fuels and Chemicals Production from Cellulosic Biomass
The conventional biochemical platform featuring enzymatic hydrolysis involves five key steps: pretreatment, cellulase production, enzymatic hydrolysis, fermentation, and product recovery. Sugars are produced as reactive intermediates for subsequent fermentation to fuels and chemicals. Herein, an alternative biochemical route is proposed. Pretreatment, enzymatic hydrolysis and cellulase production is consolidated into one single step, referred to as consolidated aerobic processing, and sugar aldonates are produced as the reactive intermediates for biofuels production by fermentation. In this study, we demonstrate the viability of consolidation of the enzymatic hydrolysis and cellulase production steps in the new route using Neurospora crassa as the model microorganism and the conversion of cellulose to ethanol as the model system. We intended to prove the two hypotheses: 1) cellulose can be directed to produce cellobionate by reducing β-glucosidase production and by enhancing cellobiose dehydrogenase production; and 2) both of the two hydrolysis products of cellobionate—glucose and gluconate—can be used as carbon sources for ethanol and other chemical production. Our results showed that knocking out multiple copies of β-glucosidase genes led to cellobionate production from cellulose, without jeopardizing the cellulose hydrolysis rate. Simulating cellobiose dehydrogenase over-expression by addition of exogenous cellobiose dehydrogenase led to more cellobionate production. Both of the two hydrolysis products of cellobionate: glucose and gluconate can be used by Escherichia coli KO 11 for efficient ethanol production. They were utilized simultaneously in glucose and gluconate co-fermentation. Gluconate was used even faster than glucose. The results support the viability of the two hypotheses that lay the foundation for the proposed new route
Clever girl: Benevolent sexism and cardiovascular threat
Benevolent sexism is a double-edged sword that uses praise to maintain gender inequality, which consequently makes women feel less efficacious, agentic and competent. This study investigated whether benevolently sexist feedback that was supportive could result in cardiovascular responses indicative of threat (lower cardiac output/higher total peripheral resistance). Women received either supportive non-sexist or supportive yet benevolent sexist feedback from a male evaluator following practice trials on a verbal reasoning test. As expected, women receiving benevolent sexist feedback exhibited cardiovascular threat during a subsequent test, relative to women receiving non-sexist feedback. There was no support for an alternative hypothesis that benevolent sexist feedback would lead to cardiovascular responses consistent with disengaging from the task altogether (i.e., lower heart rate and ventricular contractility). These findings illustrate that the consequences of benevolent sexism can occur spontaneously, while women are engaged with a task, and when the sexist feedback is intended as supportive
Predictors of Opposition to and Support for the Ordination of Women: Insights from the LDS Church
Female ordination is an important component of gender equality in Christian religious communities. While prior research has examined societal, institutional, and organisational factors that predict changes in ordination policies in Christian denominations, there is very little research examining individuals’ attitudes regarding the ordination of women. In this research, we use data from two surveys of members of The Church of Jesus Christ of Latter-day Saints (LDS) to examine the factors that predict attitudes towards the ordination of women using two polity-specific measures of support or opposition. Results indicate greater support for the ordination of women when framed in a way that takes into account LDS church governance. While several demographic variables predict attitudes towards ordination, the strongest correlate is perceptions of inequality
Germ-line transformation of the Queensland fruit fly, Bactrocera tryoni, using a piggyBac vector in the presence of endogenous piggyBac elements
We report the heritable germ-line transformation of the Queensland fruit fly, Bactrocera tryoni, using a piggyBac vector marked with either the fluorescent protein DsRed or EGFP. A transformation frequency of 5–10% was obtained. Inheritance of the transgenes has remained stable over more than 15 generations despite the presence of endogenous piggyBac sequences in the B. tryoni genome.
The sequence of insertion sites shows the usual canonical pattern of piggyBac integraton into TTAA target sites. An investigation of endogenous piggyBac elements in the B. tryoni genome reveals the presence of sequences almost identical to those reported recently for the B. dorsalis complex of fruit flies and two noctuid moths, suggesting a common origin of piggyBac sequences in these
species. The availability of transformation protocols for B. tryoni has the potential to deliver improvements in the performance of the Sterile Insect Technique for this pest species