Unsuccessful Cassava Brown Streak Disease (CBSD) evaluation attempts in western Democratic Republic of Congo and implications with cassava root necrosis disease (CRND) etiology

Open Access ArticleCassava brown streak disease (CBSD) is the second most important virus disease after Cassava mosaic disease (CMD), infecting cassava (ManihotesculetaCrantz) in Africa. The disease is caused by two distinct viruses, Cassava brown streak virus [2, 3] and Ugandan Cassava brown streak virus (family, Potyviridae: genus, Ipomovirus). Transmission of CBSV from one plant to another is reported to occur through grafting CBSV-free with infected cuttings and subsequent dissemination by infected cuttings. The basic approach to control of CBSD is selecting planting material from symptomless mother plants. Graft inoculation is the most efficient and effective of the techniques for CBSD virus transmission and consequently cuttings are the most effective way of the disease spreading. In early 2000s, cassava root necrosis similar to those of CBSD were reported in western provinces of Democratic Republic of Congo (RDC) (Kinshasa and Kongo Central) and up to date PCR diagnoses did not detect any causal agent related to the observed symptoms and the disease which was still referred as ‘CBSD-like disease’. Due to lack of molecular data and the similarity of root symptoms with CBSD, the existence of a virus has always been suspected to be the cause of CBSD-like propagation. Thus, 2 field experiments were proposed in order to verify the existence of a systematic transmission of a possible CBSD related virus, knowing that CBSD viruses are transmitted efficiently by cuttings. The first trial focused on the field evaluation of CBSD – like infected and apparently uninfected planting materials, while the second trial involved the importation of tanzanian CBSD resistant genotypes for evaluation in INERA Mvuazi research center under CBSD-like infection conditions. Results of the first trial did not show a systemic transmission of any CBSD-like pathogen while CBSD-resistant parents involved in the second trial all succumbed to CBSD-like disease

Radiative Neutralino Decay in Supersymmetric Models

The radiative decay Z2-> Z1 gamma proceeds at the one-loop level in the MSSM. It can be the dominant decay mode for the second lightest neutralino Z2 in certain regions of parameter space of supersymmetric models, where either a dynamical and/or kinematic enhancement of the branching fraction occurs. We perform an updated numerical study of this decay mode in both the minimal supergravity model (mSUGRA) and in the more general MSSM framework. In mSUGRA, the largest rates are found in the ``focus point'' region, where the mu parameter becomes small, and the lightest neutralinos become higgsino-like; in this case, radiative branching fraction can reach the 1% level. Our MSSM analysis includes a scan over independent positive and negative gaugino masses. We show branching fractions can reach the 10-100% level even for large values of the parameter tan(beta). These regions of parameter space are realized in supergravity models with non-universal gaugino masses. Measurement of the radiative neutralino branching fraction may help pin down underlying parameters of the fundamental supersymmetric model.Comment: 19 page JHEP file with 8 PS figures; previous version contained figure misplacemen

Attempts to identify Cassava Brown Streak Virus in western Democratic Republic of Congo

Open Access ArticleRoot necrosis similar to those of the cassava brown streak disease (CBSD) were observed on cassava in western provinces of the Democratic Republic of Congo (DR.Congo) in the early 2000’s. However molecular laboratory diagnosis were not able to detect any causative agent responsible for the attacks, hence, the disease related to these symptoms was named CBSD-like disease. In order to assess the distribution and the incidence of the CBSD-like disease, surveys were carried out in four western provinces, comprising, Kwango and Kwilu, Sud Ubangi, Kinshasa and Kongo Central. CBSD-like disease was observed in all surveyed provinces on the basis of root symptoms because foliar symptoms were different to those of the documented cases of CBSD in other parts of east Africa. CBSD-like disease incidence was high in Kongo Central and Sud Ubangi, exceeding an average of 50 %, but low in Kwango and Kwilu (32.8%) and in Kinshasa (19.1%). During the surveys, cassava leaf samples were collected for lab identification of the causal agent. PCR diagnosis was done on these samples using primers specific for the two known CBSVs. All samples tested negative with no amplification of DNA fragments of the correct size. Thus, further analysis on the causative organism is needed using Next Generation Sequencing (NGS) approaches. NGS approaches will help also to identify the causative organism in other Central Africa countries (Angola, Congo-Brazzaville and Gabon) where such cassava root necrosis have been reported or are suspected

Probing Slepton Mass Non-Universality at e^+e^- Linear Colliders

There are many models with non-universal soft SUSY breaking sfermion mass parameters at the grand unification scale. Even in the mSUGRA model scalar mass unification might occur at a scale closer to M_Planck, and renormalization effects would cause a mass splitting at M_GUT. We identify an experimentally measurable quantity Delta that correlates strongly with delta m^2 = m^2_{selectron_R}(M_GUT) - m^2_{selectron_L}(M_GUT), and which can be measured at electron-positron colliders provided both selectrons and the chargino are kinematically accessible. We show that if these sparticle masses can be measured with a precision of 1% at a 500 GeV linear collider, the resulting precision in the determination of Delta may allow experiments to distinguish between scalar mass unification at the GUT scale from the corresponding unification at Q ~ M_Planck. Experimental determination of Delta would also provide a distinction between the mSUGRA model and the recently proposed gaugino-mediation model. Moreover, a measurement of Delta (or a related quantity Delta') would allow for a direct determination of delta m^2.Comment: 15 pages, RevTeX, 4 postscript figure

Phytochemical composition, and analgesic and antiinflammatory properties of essential oil of Chamaemelum nobile (Asteraceae L All) in rodents

Purpose: To investigate the in vivo analgesic and anti-inflammatory activities of essential oil of dried flowers of Chamaemelum nobile (Asteraceae L. All) in Swiss mice and Wistar rats, respectively.Methods: The volatile oil of the dried flowers of C. nobile obtained by hydrodistillation was analyzed by gas chromatography-mass spectrometry (GC-MS). Animals were assigned to the following experimental groups: C. nobile (180 mg/kg), ibuprofen (100 mg/kg) and vehicle-treated groups. Chemical and thermal pain models were used for the antinociceptive study in mice while fresh egg albumin-induced acute inflammation model in rats was used for anti-inflammatory study of the essential oil.Results: The most abundant components of the oil were α-bisabolol (50 %) and farnesene (5.35 %). Oral administration of essential oil (180 mg/kg, po) significantly (p < 0.05) reduced pain and prevented inflammation in the different test models used in this study.Conclusion: The essential oil of the dried flowers of C. nobile shows in vivo analgesic and antiinflammatory activities in rodents, and therefore, possesses potentials for development into effective analgesic and anti-inflammatory agent.Keywords: Chamaemelum nobile, Asteracea, essential oil, anti-inflammatory, antinociceptiv

Spectroscopic rotational velocities of brown dwarfs

We have obtained projected rotation velocities (vsini) of a sample of 19 ultracool dwarfs with spectral types in the interval M6.5-T8 using high-resolution, near-infrared spectra obtained with NIRSPEC and the Keck II telescope. Among our targets there are two young brown dwarfs, two likely field stars, and fifteen likely brown dwarfs (30-72 Mjup) of the solar neighborhood. Our results indicate that the T-type dwarfs are fast rotators in marked contrast to M-type stars. We have derived vsini velocities between <15 and 40 km/s for them, and have found no clear evidence for T dwarfs rotating strongly faster than L dwarfs. However, there is a hint for an increasing lower envelope on moving from mid-M to the L spectral types in the vsini-spectral type diagram that was previously reported in the literature; our vsini results extend it to even cooler types. Assuming that field brown dwarfs have a size of 0.08-0.1 Rsol, we can place an upper limit of 12.5 h on the equatorial rotation period of T-type brown dwarfs. In addition, we have compared our vsini measurements to spectroscopic rotational velocities of very young brown dwarfs of similar mass available in the literature. The comparison, although model-dependent, suggests that brown dwarfs lose some angular momentum during their contraction; however, their spin down time seems to be significantly longer than that of solar-type to early-M stars.Comment: Accepted for publication in ApJ (revised version

Pregnancy complications and adverse birth outcomes among women with celiac disease: a population-based study from England

OBJECTIVES: Evidence-based information about adverse birth outcomes and pregnancy complications is crucial when counseling women with celiac disease (CD); however, limited population-based data on such risks exist. We estimated these for pregnant women with CD diagnosed before and after delivery. METHODS: We included all singleton pregnancies between 1997 and 2012 using linked primary care data from the Clinical Practice Research Datalink and secondary care Hospital Episode Statistics data. Risks of pregnancy complications (antepartum and postpartum hemorrhage, pre-eclampsia, and mode of delivery) and adverse birth outcomes (preterm birth, stillbirth, and low birth weight) were compared between pregnancies of women with and without CD using logistic/multinomial regression. Risks were stratified on the basis of whether women were diagnosed or yet undiagnosed before delivery. RESULTS: Of 363,930 pregnancies resulting in a live birth or stillbirth, 892 (0.25%) were among women with CD. Diagnosed CD was not associated with an increased risk of pregnancy complications or adverse birth outcomes compared with women without CD. However, the risk of postpartum hemorrhage and assisted delivery was slightly higher among pregnant women with diagnosed CD (adjusted odds ratio (aOR)=1.34). We found no increased risk of any pregnancy complication among those with undiagnosed CD. We only observed a 1% absolute excess risk of preterm birth and low birth weight among undiagnosed CD mothers corresponding to aOR=1.24 (95% confidence interval (CI)=0.82–1.87) and aOR=1.36 (95% CI=0.83–2.24), respectively. CONCLUSIONS: Whether diagnosed or undiagnosed during pregnancy, CD is not associated with a major increased risk of pregnancy complications and adverse birth outcomes. These findings are reassuring to both women and clinicians

Transport of Cytoplasmically Synthesized Proteins into the Mitochondria in a Cell Free System from Neurospora crassa

Synthesis and transport of mitochondrial proteins were followed in a cell-free homogenate of Neurospora crassa in which mitochondrial translation was inhibited. Proteins synthesized on cytoplasmic ribosomes are transferred into the mitochondrial fraction. The relative amounts of proteins which are transferred in vitro are comparable to those transferred in whole cells. Cycloheximide and puromycin inhibit the synthesis of mitochondrial proteins but not their transfer into mitochondria. The transfer of immunoprecipitable mitochondrial proteins was demonstrated for matrix proteins, carboxyatractyloside-binding protein and cytochrome c. Import of proteins into mitochondria exhibits a degree of specificity. The transport mechanism differentiates between newly synthesized proteins and preexistent mitochondrial proteins, at least in the case of matrix proteins. In the cell-free homogenate membrane-bound ribosomes are more active in the synthesis of mitochondrial proteins than are free ribosomes. The finished translation products appear to be released from the membrane-bound ribosomes into the cytosol rather than into the membrane vesicles. The results suggest that the transport of cytoplasmically synthesized mitochondrial proteins is essentially independent of cytoplasmic translation; that cytoplasmically synthesized mitochondrial proteins exist in an extramitochondrial pool prior to import; that the site of this pool is the cytosol for at least some of the mitochondrial proteins; and that the precursors in the extramitochondrial pool differ in structure or conformation from the functional proteins in the mitochondria

Acute toxicity study and prevention of Nω-nitro-L-arginine methyl ester-induced hypertension by Osteopermum imbricatum

Purpose: To determine the phytochemical content, toxicity and hypertension prevention effects of Osteospermum imbricatum leaf and root extracts.Methods: Phytochemical screening of leaf and root extracts of O. imbricatum was performed by colorimetric procedure. Acute toxicity of leaf and root extracts was conducted in two phases; phase I consisted of three groups of mice (n = 3) for each dose level of 10, 100 and 1000 mg/kg while phase II had three mice that received 1600, 2900 or 5000 mg/kg. Wistar rats were grouped into 7 groups that were co-treated with L-NAME and normal saline or L-NAME and hydroethanolic leaf extract (150 and 300 mg/kg) or L-NAME and hydroethanolic root extract (150 and 300 mg/kg) or L-NAME and amlodipine (5 mg/kg) or normal saline only for 4 weeks. Treatment was carried out via the oral route while blood pressure was measured weekly for 4 weeks by non-invasive tail cuff method.Results: The phytochemical profile of the leaf and root extracts revealed the presence of phenols, terpenoids, flavonoids, glycosides, tannins, steroids and saponins. Both the leaf and root extracts were toxic at 5000 mg/kg with an LD50 of 3807.89 mg/kg. In the fourth week of the study, only the leaf extract significantly (p &lt; 0.01) prevented the progression of L-NAME induced hypertension; systolic and diastolic blood pressure of the group treated with L-NAME and leaf extract (300 mg/kg) were 183 ± 1 and 140 ± 1 mmHg, respectively, compared to the group that was treated with L-NAME and normal saline which produced systolic and diastolic BP values of 213 ± 3 and 172 ± 4 mmHg, respectively. The extracts, especially OIR300, exhibited diuretic effects in the second and third week of study by promoting excretion of 16 and 19 ml urine, respectively, compared to 11 and 14 ml for LN group.Conclusion: The results suggest that O. imbricatum is moderately toxic at a high dose and contains a wide range of phytochemicals which offer partial protection against the development of nitric oxide deficiency hypertension.Keywords: Hypertension, Phytochemicals, Acute toxicity, Nω-Nitro-L-arginine methyl ester (L-NAME), Osteospermum imbricatu

Optical linear polarization in ultra cool dwarfs: A tool to probe dust in the ultra cool dwarf atmospheres

Aims.Recent studies have detected linear polarization in L dwarfs in the optical I band. Theoretical models have been developed to explain this polarization. These models predict higher polarization at shorter wavelengths. We discuss the polarization in the R and I band of 4 ultra cool dwarfs. Methods.We report linear polarization measurements of 4 ultra cool dwarfs in the R and I bands using the Intermediate dispersion Spectrograph and Imaging System (ISIS) mounted on the 4.2m William Herschel Telescope (WHT). Results.As predicted by theoretical models, we find a higher degree of polarization in the R band when compared to polarization in the I band for 3/4 of these ultra cool dwarfs. This suggests that dust scattering asymmetry is caused by oblateness >.We also show how these measurements fit the theoretical models. A case for variability of linear polarization is found, which suggests the presence of randomly distributed dust clouds. We also discuss one case for the presence of a cold debris disk.Comment: 7 pages, 2 figure