Genetic Identity and Diversity of Apple Accessions within a Candidate Collection for the Norwegian National Clonal Germplasm Repository

In order to best conserve, as well as utilize, traditional apple germplasm in Norway, an apple heritage cultivar collection was established in Ullensvang, western Norway, which aims to become the National Clonal Germplasm Repository. The establishment of the apple heritage cultivar collection was preceded by a molecular study that aimed to genotype a large number of apple accessions maintained in various ex situ sites in western and south-eastern Norway, using a rather small set of eight SSR markers. However limited, the marker set managed to identify synonyms, homonyms, and duplicates within and among the investigated collections. In this study, 171 apple accessions from the Ullensvang apple heritage cultivar collection were genotyped using a set of 20 different SSR markers. Approximately half of the accessions have been previously genotyped using eight SSR markers, enabling an assessment of whether the use of a larger marker set would yield a more accurate characterization. Based on the obtained molecular data, the apple heritage cultivar collection was determined to hold a key part of the overall genetic diversity of the Norwegian apple germplasm. Furthermore, the twelve additional SSR markers were able to differentiate several accessions groups originally thought to be synonyms, as well as to provide a more detailed insight into the genetic structure of this germplasm. © 2022 by the authors

Reproductive biology traits affecting productivity of sour cherry

The objective of this work was to evaluate variability in reproductive biology traits and the correlation between them in genotypes of 'Oblacinska' sour cherry (Prunus cerasus). High genetic diversity was found in the 41 evaluated genotypes, and significant differences were observed among them for all studied traits: flowering time, pollen germination, number of fruiting branches, production of flower and fruit, number of flowers per bud, fruit set, and limb yield efficiency. The number of fruiting branches significantly influenced the number of flower and fruit, fruit set, and yield efficiency. In addition to number of fruiting branches, yield efficiency was positively correlated with fruit set and production of flower and fruit. Results from principal component analysis suggested a reduction of the reproductive biology factors affecting yield to four main characters: number and structure of fruiting branches, flowering time, and pollen germination. Knowledge of the reproductive biology of the 'Oblacinska' genotypes can be used to select the appropriate ones to be grown or used as parents in breeding programs. In this sense, genotypes II/2, III/9, III/13, and III/14 have very good flower production and satisfactory pollen germination

Prolonged maternal separation induces undernutrition and systemic inflammation with disrupted hippocampal development in mice

Objective: Prolonged maternal separation (PMS) in the first 2 wk of life has been associated with poor growth with lasting effects in brain structure and function. This study aimed to investigate whether PMS-induced undernutrition could cause systemic inflammation and changes in nutrition-related hormonal levels, affecting hippocampal structure and neurotransmission in C57BL/6J suckling mice. Methods: This study assessed mouse growth parameters coupled with insulin-like growth factor-1 (IGF-1) serum levels. In addition, leptin, adiponectin, and corticosterone serum levels were measured following PMS. Hippocampal stereology and the amino acid levels were also assessed. Furthermore, we measured myelin basic protein and synapthophysin (SYN) expression in the overall brain tissue and hippocampal SYN immunolabeling. For behavioral tests, we analyzed the ontogeny of selected neonatal reflexes. PMS was induced by separating half the pups in each litter from their lactating dams for defined periods each day (4 h on day 1, 8 h on day 2, and 12 h thereafter). A total of 67 suckling pups were used in this study. Results: PMS induced significant slowdown in weight gain and growth impairment. Significant reductions in serum leptin and IGF-1 levels were found following PMS. Total CA3 area and volume were reduced, specifically affecting the pyramidal layer in PMS mice. CA1 pyramidal layer area was also reduced. Overall hippocampal SYN immunolabeling was lower, especially in CA3 field and dentate gyrus. Furthermore, PMS reduced hippocampal aspartate, glutamate, and gammaaminobutyric acid levels, as compared with unseparated controls. Conclusion: These findings suggest that PMS causes significant growth deficits and alterations in hippocampal morphology and neurotransmission.This work was supported in part by National Institutes of Health (NIH) research grant 5R01HD053131, funded by the Eunice Kennedy Shriver National Institute of Child Health and Human Development and the NIH Office of Dietary Supplements, and Brazilian grants from CNPq and CAPES (Grant # RO1 HD053131). The authors would like to thank Dr. Patricia Foley for veterinarian technical support and Dr. Jose Paulo Andrade for the excellent comments and suggestions to improve this manuscript. N.S. contributed with the stereological studies. I.L.F. and R.B.O. contributed with the behavioral studies. I.L.F., R.B.O., and R.L.G. contributed with the study design, study analysis, and manuscript preparation. G.A.M. and P.B.F. contributed with neurochemical brain analyses. J.I.A.L. and G.M.A. contributed with hormonal and CRP serum analyses. D.G.C., K.M.C., and R.S.R. contributed with animal experimentation and data collection

The impact of the long - term fertilisation with mineral fertilisers on the chemical properties of vertisol (central Serbia)

© 2019, ALÖKI Kft., Budapest, Hungary. The study is based on establishing soil acidity and fertility of Vertisols after the mineral fertilisers had continuously been applied from 1984 to 2016. The two phosphorus (35 and 70 kg P ha-1), the invariable nitrogen (120 kg N ha-1) and potassium (66.4 kg K ha-1) doses were used in Kragujevac location (central Serbia) for over 33 study years. The impact of fertilisation with mineral fertilisers on soil acidity, organic carbon content, the total N, P and K as well as on P, K forms balance and on some microelements (Fe, Mn, Cu and Zn) availability was studied at soil depths of 0-20 and 20-40 cm. After 33 years, the highest changes were noticed in the surface layer (0-20 cm), with an increase in the acidity level and a decrease in organic carbon content and in the total N. Simultaneously, a higher P and K content and a higher Fe and Mn mobility were established in the same soil layer whereas mineral fertilisers had no significant effect on the changes in chemical properties at 20-40 cm deep soils

An analysis of the profitability of the restitution of sessile oak forests affected by wildfires in Serbia

Wildfires caused by climate change in Europe have numerous and long-lasting ecological, economic, and social implications for sustainable development (natural environment, economy, and society as a whole). Artificial revitalization of sessile oak forests destroyed by wildfires in Serbia is feasible, but it is quite expensive compared to the spontaneous regeneration. Cost-benefit analysis (an economic approach to comparing and estimating the benefits and shortcomings of a particular business plan, i.e., an investment project by analyzing its costs and benefits) is aimed at finding the best solutions and making the best decisions about the desirability of a project. It was concluded that the investment in the restitution of sessile oak forests could be profitable only if it was based on wood production. Wood production may bear slightly higher interest rates compared to previous estimates which ranged mainly around 3%. This primarily applies to better site classes, while poor quality sites require lower rates. At lower discount rates, it takes more time to reach the break-even point, while the period of time needed to reach the break-even point shortens with higher discount rates. The length of the production cycle plays an important role in determining the investment profitability, especially regarding the relationship between the length of the production cycle and production targets (the type and the quality of wood assortments produced). According to the results of the analysis of intangible benefits and costs, the costs outweigh the benefits, which makes the restitution of sessile oak forests destroyed by fires unacceptable

TUBER YIELD AND EVAPOTRANSPIRATION OF POTATO DEPENDING ON SOIL MATRIC POTENTIAL

Abstract AKSIC, M., S. GUDZIC, N. DELETIC, N. GUDZIC, S. STOJKOVIC and J. KNEZEVIC, 2014. Tuber yield and evapotranspiration of potato depending on soil matric potential. Bulg. J. Agric. Sci., This study has been carried out through field trials with irrigation in the river valley of Southern Morava, near Nis, at the alluvium soil type, in the period [2008][2009]. The experimental field consisted of three treatments with irrigation (soil matric potential of 20, 30 and 40 kPa), as well as unirrigated control. Irrigation schedule was determined by tensiometers based on the observed soil matric potential values. The highest potato tuber yield (48.30 t ha . The data concerning tuber yield, evapotranspiration and WUE of potato point to the fact that, in order to reach high potato tuber yield and quality, one ought to keep soil matric potential at levels around 30 kPa for the conditions of south Serbia

Analysis and characterisation of phytochemicals in mulberry (Morus alba L.) fruits grown in Vojvodina, North Serbia

In this study, the polyphenolic profile of 11 Morus alba fruits grown in the Vojvodina region was investigated. Ultra high performance liquid chromatography (UHPLC) coupled with Linear Trap Quadrupole and OrbiTrap mass analyzer, and UHPLC coupled with a diode array detector and a triple-quadrupole mass spectrometer were used for the identification and quantification of the polyphenols, respectively. A total of 14 hydroxycinnamic acid esters, 13 flavonol glycosides, and 14 anthocyanins were identified, in the extracts with different distributions and contents according to the sampling. The total phenolic content ranged from 43.84 to 326.29 mg GAE/100 g frozen fruit. The radical scavenging capacity (50.18-86.79%), metal chelating ability (0.21-8.15%), ferric ion reducing power (0.03-38.45 mu M ascorbic acid) and superoxide anion radical scavenging activity (16.53-62.83%) were assessed. The findings indicated that mulberry polyphenolics may act as potent superoxide anion radical scavengers and reducing agent

Maternal Deprivation in Rats Decreases the Expression of Interneuron Markers in the Neocortex and Hippocampus

Early life stress has profound effects on the development of the central nervous system. We exposed 9-day-old rat pups to a 24 h maternal deprivation (MD) and sacrificed them as young adults (60-day-old), with the aim to study the effects of early stress on forebrain circuitry. We estimated numbers of various immunohistochemically defined interneuron subpopulations in several neocortical regions and in the hippocampus. MD rats showed reduced numbers of parvalbumin-expressing interneurons in the CA1 region of the hippocampus and in the prefrontal cortex, compared with controls. Numbers of reelin-expressing and calretinin-expressing interneurons were also reduced in the CA1 and CA3 hippocampal areas, but unaltered in the neocortex of MD rats. The number of calbinin-expressing interneurons in the neocortex was similar in the MD rats compared with controls. We analyzed cell death in 15-day-old rats after MD and found no difference compared to control rats. Thus, our results more likely reflect the downregulation of markers than the actual loss of interneurons. To investigate synaptic activity in the hippocampus we immunostained for glutamatergic and inhibitory vesicular transporters. The number of inhibitory synapses was decreased in the CA1 and CA3 regions of the hippocampus in MD rats, with the normal number of excitatory synapses. Our results indicate complex, cell type-specific, and region-specific alterations in the inhibitory circuitry induced by maternal deprivation. Such alterations may underlie symptoms of MD at the behavioral level and possibly contribute to mechanisms by which early life stress causes neuropsychiatric disorders, such as schizophrenia