Felt senses of self and no-self in therapy

The thesis develops Gendlin's concept of the felt sense in two directions, and introduces parallel concepts of self. It starts by examining western and eastern cultural contexts, neuroscientific conceptualisations and linguistic issues as they relate to self, using the lens of Gendlin's two ways of relating to the world ̶ interpreting according to the unit model and thinking beyond patterns, to point out conceptual confusions. Buddhist philosophy and practice are discussed as methods of undoing such conceptual confusions in order to relieve suffering, with self as an independent, stable, substantial entity being the primary example of such a confusion. Dualism is identified as the basic misconception from which suffering ensues. Non-duality is investigated as a spiritual endstate, an integral part of the goals of humanistic therapies and an intrinsic element in 'carrying forward', then compared with Gendlin's implicit intricacy, Sartre's Being-in-itself and intersubjective theories. A small qualitative study investigates what happens when felt senses of self are intentionally produced or accessed by focusing. A continuum of experiences is described, ranging from self to no-self, with trauma proving a major block to both self and no-self experiencings. The felt sense is re-defined in two ways, as an extending boundary and as a direct referent. A sense of self is also considered both as a boundary drawing exercise, and a direct referent. Self may function in either of these forms on a relative level, constructively or destructively, according to circumstances and conditions, while on an ontological level no such single entity may be proven to exist. The conclusion is drawn that self and no-self form a kind of twisting human thread, which shows, at any one moment, just one side of a duality. These sides are conceptually, rather than actually, distinct

Cytotoxicity and Oxidative Stress Induction by Root Canal Sealers in Human Periodontal Ligament Fibroblasts: An in vitro Study

Introduction: The aim of the study was to investigate the in vitro cytotoxicity, the profile of cell death, and the level of oxidative stress in human periodontal ligament fibroblasts (HPdLFs) after exposure to selected root canal sealers. Methods and Materials: Freshly mixed or set Endomethasone N (EN), RealSeal (RSEAL), Roeko Seal Automix (RSA), and Sealapex (SP) were incubated with HPdLFs. Fluorescein isothiocyanate (FITC)-annexin V (AnV) and propidium iodide (PI) staining followed by flow cytometry was used to identify the effects of the materials on cell viability and the profile of cell death. 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA) with fluorescence-activated cell sorting was used to determine reactive oxygen species (ROS) formation in HPdLFs. Statistical analyses were performed using one-way ANOVA followed by post hoc tests, and significance was determined at P<0.05. Results: All materials reduced the viability of the cultured cells compared with the controls (P<0.05). Fresh SP and EN, and set RSA generated an increase of necrotic cells (P<0.05), whilst fresh RSEAL and RSA induced an elevation of apoptotic cells (P<0.001). Set RSEAL caused a rise in both apoptotic and necrotic cells compared with the controls (P<0.05). Fresh EN, RSEAL, and SP resulted in increased intracellular ROS generation compared with the negative control (P<0.001), whilst fresh RSA and all set materials were ineffective. Conclusions: This in vitro study showed us the materials tested were characterized by differentiated cytotoxic effects on HPdLFs. The fresh and set forms of sealers were capable of eliciting toxic action, inducing apoptosis and/or necrosis in HPdLFs. The toxic effects of fresh EN, RSEAL, and SP might have been due to the induction of oxidative stress in human periodontal fibroblasts. The cytotoxicity of RSA seemed to be related to the involvement of other mechanisms

Hybrids and the Boundaries of Moral Considerability or Revisiting the Idea of Non-Instrumental Value

The transgressive ontological character of hybrids—entities crossing the ontological binarism of naturalness and artificiality, e.g., biomimetic projects—calls for pondering the question of their ethical status, since metaphysical and moral ideas are often inextricably linked. The example of it is the concept of “moral considerability” and related to it the idea of “intrinsic value” understood as a non-instrumentality of a being. Such an approach excludes hybrids from moral considerations due to their instrumental character. In the paper, we revisit the boundaries of moral considerability by reexamining the legitimacy of identifying intrinsic value with a non-instrumental one. We offer the concept of “functional value,” which we define as a simultaneous contribution to the common good of the ecosystem and the possibility to disclose the full variety of aspects of a being’s identity. We argue that such a value of hybrids allows us to include them into the scope of moral considerability.</p

Evaluation of cytotoxicity and pH changes generated by various dental pulp capping materials — an in vitro study

Introduction. Various materials are used in direct dental pulp capping method. Their biocompatibility and alkalizing abilities are of primary importance affecting therapeutic effects. The aim of this study was to evaluate and compare the cytotoxicity of various pulp-capping materials on human gingival fibroblasts and investigate the pH changes induced by these materials. Material and methods. Human gingival fibroblasts were cultured with nine direct pulp materials using culture plate inserts. The cytotoxic effects were recorded by using an MTT-based colorimetric assay after 3 and 24 h. In the second part of the experiment, the materials were inserted in dialysis tubes and transferred into plastic vials containing deionized water. The changes of the medium pH were measured after 3 and 24 h. Results. We showed differences in cell viability of gingival fibroblasts after varied time of exposition for the tested materials. Cell viability after 24 h increased for Dycal, Biopulp, and Calcipro, and decreased for Calcipulpe, Angelus, Angelus White, and ProRoot Regular. Cell viability for ProRoot and Life did not change. Non-setting calcium hydroxide preparations followed by the MTA group and setting calcium hydroxide materials produced the highest pH. All the tested materials significantly increased pH (p &lt; 0.0001) at 24 h. Conclusions. Currently used pulp capping materials varied in their cytotoxicity relative to human gingival fibroblasts and their alkalizing capacities. Since most likely pH does not affect the viability of cultured cells, further investigations are required to determine physicochemical properties of these materials and the biological activity of the dental pulp

Wild food plant use in 21st century Europe: The disappearance of old traditions and the search for new cuisines involving wild edibles

The aim of this review is to present an overview of changes in the contemporary use of wild food plants in Europe, mainly using the examples of our home countries: Poland, Italy, Spain, Estonia and Sweden. We set the scene referring to the nutrition of 19th century peasants, involving many famine and emergency foods. Later we discuss such issues as children's wild snacks, the association between the decline of plant knowledge and the disappearance of plant use, the effects of over-exploitation, the decrease of the availability of plants due to ecosystem changes, land access rights for foragers and intoxication dangers. We also describe the 20th and 21st century vogues in wild plant use, particularly their shift into the domain of haute-cuisine. © The Author(s) 2012

Crustal noble gases in deep brines as natural tracers of vertical transport processes in the Michigan Basin

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/94594/1/ggge1543.pd

New audiences, international distribution, and translation

The interconnectivity made possible by the technological advancements of the past three decades has changed the way how audiences engage with audiovisual content around the world. On the one hand, viewers have become empowered consumers who are also engaged in the distribution of content; on the other, companies serving global audiences have emerged as key players in the audiovisual market. With more access to content, through piracy or official channels, new consumption habits, such as binge watching, have become common among viewers. Non-professional subtitling has played a key role in the expansion of the audiovisual market, the configuration of international audiences and the development of new viewing traditions. By looking at non-professional subtitling as a constituent of the international media flows, this chapter proposes Translation Studies should look at the reception of non-professional subtitles at a global scale to understand the interplay between non-professional subtitling, its producers/users and the audiovisual market, as well as the societal impact of the phenomenon

Comparison of some chemical parameters of a naturally debittered olive (Olea europaea L.) type with regular olive varieties

Some olives grown in Karaburun peninsula in the west part of Turkey and mostly coming from Erkence variety lose their bitterness while still on the tree and are called Hurma among locals. This olive type does not require further processing to remove the bitter compounds. In this study, sugar, organic acid and fatty acid profiles of Hurma, Erkence (not naturally debittered) and Gemlik (commonly consumed as table olive) olives were determined throughout 8 weeks of maturation period for two consecutive harvest seasons, and the results were analysed by principal component analysis (PCA). PCA of sugar and organic acid data revealed a differentiation in terms of harvest year but not on variety. Hurma olive is separated from others due to its fatty acid profile, and it has higher linoleic acid content compared to others. This might be an indication of increased desaturase enzyme activity for Hurma olives during natural debittering phase.TUBITAK (TOVAG-110O780

Changes in Phospholipid Composition Studied by HPLC and Electric Properties of Liver Cell Membrane of Ethanol-Poisoned Rats

Ethanol introduced into the organism undergoes rapid metabolism to acetaldehyde and then to acetic acid. The process is accompanied by formation of reactive oxygen species (ROS), which damage mainly lipids of membrane cells. The effects of ROS can be neutralized by administering preparations with antioxidant properties. The natural preparations of this kind are teas