Transplacental transmission of field and rescued strains of BTV-2 and BTV-8 in experimentally infected sheep

Transplacental transmission of bluetongue virus has been shown previously for the North European strain of serotype 8 (BTV-8) and for tissue culture or chicken egg-adapted vaccine strains but not for field strains of other serotypes. In this study, pregnant ewes (6 per group) were inoculated with either field or rescued strains of BTV-2 and BTV-8 in order to determine the ability of these viruses to cross the placental barrier. The field BTV-2 and BTV-8 strains was passaged once in Culicoides KC cells and once in mammalian cells. All virus inoculated sheep became infected and seroconverted against the different BTV strains used in this study. BTV RNA was detectable in the blood of all but two ewes for over 28 days but infectious virus could only be detected in the blood for a much shorter period. Interestingly, transplacental transmission of BTV-2 (both field and rescued strains) was demonstrated at high efficiency (6 out of 13 lambs born to BTV-2 infected ewes) while only 1 lamb of 12 born to BTV-8 infected ewes showed evidence of in utero infection. In addition, evidence for horizontal transmission of BTV-2 between ewes was observed. As expected, the parental BTV-2 and BTV-8 viruses and the viruses rescued by reverse genetics showed very similar properties to each other. This study showed, for the first time, that transplacental transmission of BTV-2, which had been minimally passaged in cell culture, can occur; hence such transmission might be more frequent than previously thought

Large adiabatic temperature and magnetic entropy changes in EuTiO3

We have investigated the magnetocaloric effect in single and polycrystalline samples of quantum paraelectric EuTiO3 by magnetization and heat capacity measurements. Single crystalline EuTiO3 shows antiferromagnetic ordering due to Eu2+ magnetic moments below TN = 5.6 K. This compound shows a giant magnetocaloric effect around its Neel temperature. The isothermal magnetic entropy change is 49 Jkg-1K-1, the adiabatic temperature change is 21 K and the refrigeration capacity is 500 JKg-1 for a field change of 7 T at TN. The single crystal and polycrystalline samples show similar values of the magnetic entropy change and adiabatic temperature changes. The large magnetocaloric effect is due to suppression of the spin entropy associated with localized 4f moment of Eu2+ ions. The giant magnetocaloric effect together with negligible hysteresis, suggest that EuTiO3 could be a potential material for magnetic refrigeration below 20 K.Comment: 12 pages, 4 figure

Association between exercise blood pressure, Na+ ingestion and Cold Pressor Test: A Pilot Study

Please view abstract in the attached PDF fil

Post-test simulations for the NACIE-UP benchmark by STH codes

This paper illustrates the results obtained in the last phase of the NACIE-UP benchmark activity foreseen inside the EU SESAME Project. The purpose of this research activity, performed by system thermal–hydraulic (STH) codes, is finalized to the improvement, development and validation of existing STH codes for Heavy Liquid Metal (HLM) systems. All the participants improved their modelling of the NACIE-UP facility, respect to the initial blind simulation phase, adopting the actual experimental boundary conditions and reducing as much as possible sources of uncertainty in their numerical model. Four different STH codes were employed by the participants to the benchmark to model the NACIE-UP facility, namely: CATHARE for ENEA, ATHLET for GRS, RELAP5-3D© for the “Sapienza” University of Rome and RELAP5/Mod3.3(modified) for the University of Pisa. Three reference tests foreseen in the NACIE-UP benchmark and carried out at ENEA Brasimone Research Centre were analysed from four participants. The data from the post-test analyses, performed independently by the participant using different STH codes, were compared together and with the available experimental results and critically discussed

Application of XeCl308nm excimer laser radiation to mutate industrial microorganisms

EnIn this study, we have investigated the effects of an XeCl308 nm excimer laser radiation on bacterial mutagenesis. Our experiments have revealed that the mutagenesis inducted by the XeCl308 nm excimer laser radiation is independent from RecA protein, the regulator of the SOS response, unlike UV254 nm radiation that is not mutagenic for Escherichia coli mutants lacking the RecA protein. This found suggests that the UV308 nm laser radiation might be mutagenic also in microorganisms naturally lacking the SOS response. To test this hypothesis, we applied our innovative mutagenesis approach on Nonomuraea ATCC 39727, an industrial strain producing an antibiotic, which is relatively refractory to UV254 nm radiation-induced mutagenesis. Our results demonstrated the efficiency of XeCl308 nm excimer laser radiation to induce mutagenesis in Nonomuraea ATCC 39727

Spaces of Memory

In the last decade, museums, memorials and monuments have become the battlefield for competing and conflicting visions of the past and the hegemonic or counter memories of the so-called “difficult heritage” or “traumatic heritage”. Far from being mere spaces of musealization that freeze and fix dominant narratives of the past, spaces of memory are increasingly turning into sites of negotiations and reconfigurations of meaning in which social and political identities are debated, strengthened, or weakened in reference to the traumatic experiences of the past which they “represent”. Yet, what does it mean to spatially represent a (traumatic) memory, and what is a space of memory? In expanding and, simultaneously, problematizing Pierre Nora’s (Nora 1996) category of lieu de mémoire, the way we think of spaces of memory aims at an in-depth examination of the peculiar yet specific ways of re-thinking the nexus between space and memory: how do we elaborate, activate, and make visible spaces for memory? This question points to the dynamic construction that underlines the production and connection of spatiality and memory, as well as to the coexistence of a plurality of meanings and experiences that characterize spaces of memory

A multilevel graph approach for IoT-based complex scenario management through situation awareness and semantic approaches

Modern reality and the environments in which we live are characterized by technology components geared toward automatic management through pervasive services. Thanks to the advent of the Internet of Things, such environments can provide information such as pollution levels, public transport conditions, efficiency of energy distribution networks, and identification of suspicious activities by generating complex scenarios. The profitable management of such scenarios can be performed through context modeling and methodologies that can extract and understand environmental information by preventing certain events through artificial intelligence techniques by increasing Situation Awareness. This paper focuses on developing a methodology with predictive capabilities and context adaptability for managing complex scenarios. The use of semantic and graph-based approaches, unlike many approaches used, leads to better integration of knowledge, resulting in improved system performance. In addition, such approaches allow understanding of what is happening in the system at a given time, enabling manipulation and integration of semantic information. Graph-based approaches chosen for this purpose are Ontologies, Context Dimension Trees, and Bayesian Networks, which are able to support the end-user or expert user in handling complex scenarios. The proposed methodology has been validated and applied to real complex scenarios based on the IoT paradigm. The proposed approach validation was conducted using open data from the city of London; a practical scenario case study was conducted in the field of automated management of a Smart Home. In both cases, the system achieved promising results

The impact of diabetes in implant oral rehabilitations: A bibliometric study and literature review

Introduction: Diabetes represents a potential risk factor for bone healing and dental implant treatment predictability. The aim of the present investigation was to perform a bibliometric evaluation of articles on the topic of the impact of diabetes on implant oral rehabilitations. Material and methods: A Boolean keywords search was performed on Scopus database and recorded the list of articles, authors and affiliations. The journal impact factor was calculated by the Journal Citation Report Clarivate electronic database. The total papers, number of citations and journal impact factors were calculated. Results: a total of 476 papers and 162 authors were assessed. The mean authors total citations were 2880.11± 4070.24 and the mean impact factor value was 1.942±1.15 Conclusions: uncontrolled diabetes impacts on dental implant rehabilitation with an increased risk of implant failure and periimplant disease in long-term rehabilitation

Magnetic field tuning of antiferromagnetic Yb3_{3}Pt4_{4}

We present measurements of the specific heat, magnetization, magnetocaloric effect and magnetic neutron diffraction carried out on single crystals of antiferromagnetic Yb$_{3}$Pt$_{4}$, where highly localized Yb moments order at $T_{\rm N}=2.4$ K in zero field. The antiferromagnetic order was suppressed to $T_{\rm N}\rightarrow 0$ by applying a field of 1.85 T in the $ab$ plane. Magnetocaloric effect measurements show that the antiferromagnetic phase transition is always continuous for $T_{\rm N}>0$, although a pronounced step in the magnetization is observed at the critical field in both neutron diffraction and magnetization measurements. These steps sharpen with decreasing temperature, but the related divergences in the magnetic susceptibility are cut off at the lowest temperatures, where the phase line itself becomes vertical in the field-temperature plane. As $T_{\rm N}\rightarrow0$, the antiferromagnetic transition is increasingly influenced by a quantum critical endpoint, where $T_{\rm N}$ ultimately vanishes in a first order phase transition.Comment: 9 pages, 6 figure

DNA damage by a single intense shot of soft X-rays emitted by a laser-produced plasma

EnA suitable plane transmission line was developed and its behaviour analysed at 900 MHz radiofrequency fields to study the DNA mutability and repair of microorganisms. In this work, utilizing such a device, we investigated the behaviour of the DNA mutability and repair of Escherichia coli strains. The transmission line was very simple and versatile in changing its characteristic resistance and field intensity by varying its sizes. In absence of cell samples inside the transmission line, the relative modulation of the electric and/or magnetic field was ±31% with respect to the mean values, allowing the processing of more samples at different exposure fields in a single run. Slight decrease in spontaneous mutability to rifampicin-resistance of the E. Coli JC411 strain, was demonstrated in mismatch-repair proficient samples exposed to the radio-frequency fields during their growth on solid medium