39 research outputs found

    Response of a Model of CO Oxidation with CO Desorption and Diffusion to a Periodic External CO Pressure

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    We present a study of the dynamical behavior of a Ziff-Gulari-Barshad model with CO desorption and lateral diffusion. Depending on the values of the desorption and diffusion parameters, the system presents a discontinuous phase transition between low and high CO coverage phases. We calculate several points on the coexistence curve between these phases. Inclusion of the diffusion term produces a significant increase in the CO_2 production rate. We further applied a square-wave periodic pressure variation of the partial CO pressure with parameters that can be tuned to modify the catalytic activity. Contrary to the diffusion-free case, this driven system does not present a further enhancement of the catalytic activity, beyond the increase induced by the diffusion under constant CO pressure.Comment: 5 pages, RevTe

    Mechanical properties and fire-resistance of composites with marble particles

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    The main aim of this work is to manufacture a composite material based on a natural material (marble) with acceptable mechanical properties and fire resistance, for being used in habitat industry as floor or wall in buildings. Marble used as raw material is the waste powder of quarry or plate manufacturing. To achieve this objective, polyester matrix composites with 50 wt.% of marble and 3 wt.% of glass fiber (short fiber or mesh) were prepared. The novelty of this study is the high percentage of ceramic material added to a polymer matrix composite and the fire resistance study. Samples were characterized mechanically through flexural test, Charpy impact test, compression test and wear resistance by pin-on-disk test. Fracture surfaces were analyzed by scanning electron microscope (SEM), and wear tracks were studied by SEM and 3D optical profilometer. Besides, samples were subjected to fire test using a Bunsen burner at 900 °C for 20 min. Sample temperature at the opposite-to-fire test side was measured with an infrared thermometer. Results show that marble improves mechanical properties of polyester and the effect of the glass fiber depends on its morphology (fiber or mesh). Fire resistance is high, and the fire goes out when the flame is turned off. Furthermore, the mesh maintains the integrity of the sample.This research received funding from the SUDOE project SOE1/P1/E307

    Hyper, a Hydrogen Peroxide Sensor, Indicates the Sensitivity of the Arabidopsis Root Elongation Zone to Aluminum Treatment

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    Emerging evidence indicates that some reactive oxygen species (ROS), such as the superoxide anion radical and hydrogen peroxide (H2O2), are central regulators of plant responses to biotic and abiotic stresses. Thus, the cellular levels of ROS are thought to be tightly regulated by an efficient and elaborate pro- and antioxidant system that modulates the production and scavenging of ROS. Until recently, studies of ROS in plant cells have been limited to biochemical assays and the use of fluorescent probes; however, the irreversible oxidation of these fluorescent probes makes it impossible to visualize dynamic changes in ROS levels. In this work, we describe the use of Hyper, a recently developed live cell probe for H2O2 measurements in living cells, to monitor oxidative stress in Arabidopsis roots subjected to aluminum treatment. Hyper consists of a circularly permuted YFP (cpYFP) inserted into the regulatory domain of the Escherichia coli hydrogen peroxide-binding protein (OxyR), and is a H2O2-specific ratiometric, and therefore quantitative, probe that can be expressed in plant and animal cells. Now we demonstrate that H2O2 levels drop sharply in the elongation zone of roots treated with aluminum. This response could contribute to root growth arrest and provides evidence that H2O2 is involved in early Al sensing

    Developmental role of the tomato Mediator complex subunit MED18 in pollen ontogeny

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    [EN] Pollen development is a crucial step in higher plants, which not only makes possible plant fertilization and seed formation, but also determines fruit quality and yield in crop species. Here, we reported a tomato T-DNA mutant, pollen deficient1 (pod1), characterized by an abnormal anther development and the lack of viable pollen formation, which led to the production of parthenocarpic fruits. Genomic analyses and the characterization of silencing lines proved that pod1 mutant phenotype relies on the tomato SlMED18 gene encoding the subunit 18 of Mediator multi-protein complex involved in RNA polymerase II transcription machinery. The loss of SlMED18 function delayed tapetum degeneration, which resulted in deficient microspore development and scarce production of viable pollen. A detailed histological characterization of anther development proved that changes during microgametogenesis and a significant delay in tapetum degeneration are associated with a high proportion of degenerated cells and, hence, should be responsible for the low production of functional pollen grains. Expression of pollen marker genes indicated that SlMED18 is essential for the proper transcription of a subset of genes specifically required to pollen formation and fruit development, revealing a key role of SlMED18 in male gametogenesis of tomato. Additionally, SlMED18 is able to rescue developmental abnormalities of the Arabidopsis med18 mutant, indicating that most biological functions have been conserved in both species. Significance Statement Pollination is a key development process in the life cycle of flowering plants. Genetic and molecular characterization of a tomato mutant have led to the identification of POD1 gene encoding the Mediator complex subunit MED18 whose function is required for tapetum tissue degeneration, a crucial step for pollen development. Furthermore, we show that MED18 fulfils an essential role in tomato, ensuring proper gene regulation during pollen ontogeny.This research was supported by the Spanish Ministry of Economy and Competitiveness (grants AGL2015-64991-C3-1-R, AGL2015-64991-C3-2-R, AGL2015-64991-C3-3-R, BIO2013-43098-R, BFU2016-77243-P and BIO2016-77559-R) and Junta de Andalucia (grant P12-AGR-1482).Pérez Martín, F.; Juan Yuste-Lisbona, F.; Pineda, B.; García Sogo, B.; Del Olmo, I.; Alché, JDD.; Egea, I.... (2018). Developmental role of the tomato Mediator complex subunit MED18 in pollen ontogeny. The Plant Journal. 96(2):300-315. https://doi.org/10.1111/tpj.14031S300315962Allen, B. L., & Taatjes, D. J. (2015). 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Critical Reviews in Plant Sciences, 16(1), 31-53. doi:10.1080/07352689709701945Helliwell, C. (2003). Constructs and methods for high-throughput gene silencing in plants. Methods, 30(4), 289-295. doi:10.1016/s1046-2023(03)00036-7Honys, D., & Twell, D. (2004). Transcriptome analysis of haploid male gametophyte development in Arabidopsis. Genome Biology, 5(11). doi:10.1186/gb-2004-5-11-r85Jeong, H.-J., Kang, J.-H., Zhao, M., Kwon, J.-K., Choi, H.-S., Bae, J. H., … Kang, B.-C. (2014). Tomato Male sterile 1035 is essential for pollen development and meiosis in anthers. Journal of Experimental Botany, 65(22), 6693-6709. doi:10.1093/jxb/eru389Jimenez-Lopez, J. C., Zienkiewicz, A., Zienkiewicz, K., Alché, J. D., & Rodríguez-García, M. I. (2015). Biogenesis of protein bodies during legumin accumulation in developing olive (Olea europaea L.) seed. Protoplasma, 253(2), 517-530. doi:10.1007/s00709-015-0830-5Kornberg, R. D. (2005). Mediator and the mechanism of transcriptional activation. Trends in Biochemical Sciences, 30(5), 235-239. doi:10.1016/j.tibs.2005.03.011Lai, Z., Schluttenhofer, C. M., Bhide, K., Shreve, J., Thimmapuram, J., Lee, S. Y., … Mengiste, T. (2014). MED18 interaction with distinct transcription factors regulates multiple plant functions. Nature Communications, 5(1). doi:10.1038/ncomms4064Larivière, L., Geiger, S., Hoeppner, S., Röther, S., Sträßer, K., & Cramer, P. (2006). Structure and TBP binding of the Mediator head subcomplex Med8–Med18–Med20. Nature Structural & Molecular Biology, 13(10), 895-901. doi:10.1038/nsmb1143Lee, S. K., Chen, X., Huang, L., & Stargell, L. A. (2013). The head module of Mediator directs activation of preloaded RNAPII in vivo. Nucleic Acids Research, 41(22), 10124-10134. doi:10.1093/nar/gkt796Li, D.-D., Xue, J.-S., Zhu, J., & Yang, Z.-N. (2017). Gene Regulatory Network for Tapetum Development in Arabidopsis thaliana. 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Pollen Semi-Sterility1 Encodes a Kinesin-1–Like Protein Important for Male Meiosis, Anther Dehiscence, and Fertility in Rice. The Plant Cell, 23(1), 111-129. doi:10.1105/tpc.109.07369

    Nurses' perceptions of aids and obstacles to the provision of optimal end of life care in ICU

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    Contains fulltext : 172380.pdf (publisher's version ) (Open Access

    Influencia de taninos sobre características físicas y sensoriales de carne de bovinos en engorda

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    Para evaluar la adición de 0.3 % extracto de taninos en las características físicas y sensoriales de la carne de bovinos engordados de forma intensiva, se utilizaron muestras del músculo Longissimus dorsi de 16 toretes ( Bos taurus x Bos indicus ), los cuales durante los últimos 70 días de la engorda recibieron dieta de finalización (13.3% PC y 2.0 Mcal ENm/kg de MS), y en grupos de ocho se asignaron al azar para recibir uno de dos tratamientos: 1) die ta de finalización (testigo); 2) testigo más 0.3% (base seca) de extracto de taninos (ET). El extracto de taninos fue de Bypro® (SilvaFeed, Indunor, S.A., Argentina) que contiene 70 % de taninos. Los toretes se sacrificaron en l a planta TIF No. 99. A las 24 h posteriores al sacrificio se tomaron cuatro muestras (2.5 cm de grosor) a la altu ra de la 12 a y 13 a costilla de la media canal izquierda. Se evaluaron las características físicas y el perfil sensorial , así como la percepción y aceptaci ón de la carne por consumidores. Los taninos no influyeron en las características físicas de la carne ( P >0.05). El perfil sensorial no fue influenciado por tratamientos ( P >0.05). La percepción y preferencia del producto respecto a jugosidad y terneza fue similar para ambos tratamientos. Los consumidores mostraron preferencia por el nivel Gusta moderadamente , para testigo y tratamiento. Se concluye que la adición de 0.3 % de extracto de taninos en la dieta de bovinos en engorda intensiva, no afecta las características físic as y sensoriales de la carne

    Influencia de la adiciòn de extracto de taninos sobre las caracterìsticas fìsicas y atributos sensoriales de la carne de bovinos engordados de forma intensiva

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    Para evaluar la adición de 0.3 % extracto de taninos en las características físicas y sensoriales de la carne de bovinosengordados de forma intensiva, se utilizaron muestras del músculo Longissimus dorside 16 toretes (Bos taurus xBosindicus), los cuales durante los últimos 70 días de la engorda recibieron dieta de finalización (13.3% PC y 2.0 McalENm/kg de MS), y en grupos de ocho se asignaron al azar para recibir uno de dos tratamientos: 1) dieta de finalización(testigo); 2) testigo más 0.3% (base seca) de extracto de taninos (ET). El extracto de taninos fue de Bypro®(SilvaFeed, Indunor, S.A., Argentina) que contiene 70 % de taninos. Los toretes se sacrificaron en la planta TIFNo. 99. A las 24 h posteriores al sacrificio se tomaron cuatro muestras (2.5 cm de grosor) a la altura de la 12ay 13acostilla de la media canal izquierda. Se evaluaron las características físicas y el perfil sensorial, así comola percepción y aceptación de la carne por consumidores. Los taninos no influyeron en las características físicasde la carne (P>0.05). El perfil sensorial no fue influenciado por tratamientos (P>0.05). La percepción y preferenciadel producto respecto a jugosidad y terneza fue similar para ambos tratamientos. Los consumidores mostraronpreferencia por el nivel “Gusta moderadamente”, para testigo y tratamiento. Se concluye que la adición de 0.3 % deextracto de taninos en la dieta de bovinos en engorda intensiva, no afecta las características físicas y sensoriales dela carneCONACYT UAEMex UAM- Iztapalapa UA
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