Anti-inflammatory, analgesic and antipyretic effects of Lepidagathis anobrya Nees (Acanthaceae)

This study investigated the general acute, anti-inflammatory, analgesic and antipyretic effects of methanol extract of Lepidagathis anobrya Nees (Acanthaceae). Carrageenan-induced rat paw edema and croton oil-induced ear edema in rats were used for the evaluation of general acute anti-inflammatory effects. Acetic acid-induced writhing response and yeastinduced hyperpyrexia in mice were used to evaluate the analgesic and antipyretic activities respectively. The extract at doses of 10, 25, 50 and 100 mgkg-1 for carrageenan test and doses of 0.5 mg/ear for croton oil test induced a significant reduction (p < 0.001) of paw and ear edemas in rats. In the analgesic and antipyretic tests, the extract has shown a significant inhibition of writhes and hyperpyrexia with all the doses used when compared to the untreated control group. These results clearly show the anti-inflammatory, analgesic and antipyretic effects of the methanol extract of Lepidagathis anobrya and give the scientific basis for its traditional use. Further studies are needed to clarify the mechanism of action and the components responsible for these pharmacological effects.Key words: Lepidagathis anobrya, anti-inflammatory, analgesic, antipyretic, acute toxicity.doi: 10.4314/ajtcam.v8i4.1

Acidification des sols ferrugineux et ferrallitiques dans les systèmes de production cotonnière au Burkina Faso

La productivité des systèmes de culture coton-céréales est menacée par la dégradation et l’acidification des terres. Afin de déterminer les causes de l’acidification des sols dans la zone cotonnière du Burkina Faso, une expérimentation a été conduite pendant deux années, en milieu paysan, sur des sols ferrugineux et ferrallitiques, respectivement sur les sites de Dohoun et Balla. Trois modes d’exploitation des terres, définis par la jachère, la culture attelée et la culture motorisée, ont été comparés pour déterminer leurs effets sur les caractéristiques physico-chimiques des sols étudiés. Par rapport à la jachère, comparée à la culture attelée, la culture motorisée a entraîné, en surface (0-20 cm) des sols ferrugineux et ferrallitiques, une augmentation des teneurs en sable de 8 à 12% et une baisse du taux d’argile de 35% qui expose ces sols à l’acidification. Contrairement à la jachère, la culture motorisée a entraîné des pertes significatives de carbone et d’azote, qui rendent les sols plus sensibles à l’acidification qu’en culture attelée. L’exploitation des terres s’est aussi traduite par une baisse significative des teneurs des cations majeurs du complexe adsorbant (Ca2+ et Mg2+), de la somme des bases échangeables (SBE) et de la capacité d’échange cationique (CEC), accentuant la vulnérabilité à l’acidification sous culture motorisée. La dégradation des sols cultivés a été plus importante pour les sols ferrallitiques qu’au niveau des sols ferrugineux, dont la CEC a été 2 à 3 fois plus élevée. La culture attelée et la culture motorisée, pratiquées, le plus souvent, sans restitution organique, se sont révélées comme étant des causes de l’acidification des sols ferrallitiques, plus accentuée que celle des sols ferrugineux. L’étude recommande d’adopter des techniques adéquates de travail du sol et de procéder à des amendements appropriés pour atténuer l’acidification et garantir la durabilité des systèmes de production.Mots clés: Exploitation des terres, acidification, sol ferrugineux, sol ferrallitique, zone cotonnière

Efficacy of the association of cover crops with maize and direct sowing short-term effect on crops? yields in maize-cotton cropping system in Western Burkina Faso.

To improve the productivity and sustainability of cotton and cereals based system, direct sow ing under mulch was tested for its efficacy on cotton and maize yields on the research station of Farako - Bâ, in Western Burkina Faso. The experimental design was a complete randomized blocks of Fisher with four replications. Conventional tillage by annual moldboard plowing (T7) was compared with direct sowing under mulch -based cropping system (DMC) using maize association with cover crop s defined as: maize without cover crop (T1), maize +Brachiaria ruziziensis(T2), maize + B. ruziziensis+ Mucuna cochinchinensis (T3), maize + B. ruziziensis+ Panicum maximum (T4), maize + B. ruziziensis + Stylosantes hamata (T5), and maize + Crotalaria juncea (T6). Cover crops were planted 21 days after maize emergence between the rows of this main crop. The biomass produced by the cover crops and maize straws were evaluated as well as maize and cotton yields, during the first 6 years of the study, from 2010 to 2015. Results showed that among cover crops, the biomass production was significantly lower with C. juncea. The associations of cover crops with maize increased significantly the production of total dry matter compared to plots without cover crops, in the conventional tillage. Association with cover crops did not influence significantly nitrogen, phosphorus and potassium contents of maize and the maize’s yields even if the depressive effects were recorded. Compared to the conventional tillage, the DMC appeared also effective on seed cotton yields even without a significant improvement during the 6 first years of the study . These promising results, confirm the feasibility in tropical conditions of DMC which must be continued to better analyze its long-term effects on soil properties

Interleukin-10 and soluble tumor necrosis factor receptor II are potential biomarkers of Plasmodium falciparum infections in pregnant women: a case-control study from Nanoro, Burkina Faso.

BACKGROUND: Diagnosis of malaria in pregnancy is problematic due to the low sensitivity of conventional diagnostic tests (rapid diagnostic test and microscopy), which is exacerbated due to low peripheral parasite densities, and lack of clinical symptoms. In this study, six potential biomarkers to support malaria diagnosis in pregnancy were evaluated. METHODS: Blood samples were collected from pregnant women at antenatal clinic visits and at delivery. Microscopy and real-time PCR were performed for malaria diagnosis and biomarker analyses were performed by ELISA (interleukin 10, IL-10; tumor necrosis factor-α, TNF-α; soluble tumor necrosis factor receptor II, sTNF-RII; soluble fms-like tyrosine kinase 1, sFlt-1; leptin and apolipoprotein B, Apo-B). A placental biopsy was collected at delivery to determine placental malaria. RESULTS: IL-10 and sTNF-RII were significantly higher at all time-points in malaria-infected women (p < 0.001). Both markers were also positively associated with parasite density (p < 0.001 and p = 0.003 for IL-10 and sTNF-RII respectively). IL-10 levels at delivery, but not during pregnancy, were negatively associated with birth weight. A prediction model was created using IL-10 and sTNF-RII cut-off points. For primigravidae the model had a sensitivity of 88.9% (95%CI 45.7-98.7%) and specificity of 83.3% (95% CI 57.1-94.9%) for diagnosing malaria during pregnancy. For secundi- and multigravidae the sensitivity (81.8% and 56.5% respectively) was lower, while specificity (100.0% and 94.3% respectively) was relatively high. Sub-microscopic infections were detected in 2 out of 3 secundi- and 5 out of 12 multigravidae. CONCLUSIONS: The combination of biomarkers IL-10 and sTNF-RII have the potential to support malaria diagnosis in pregnancy. Additional markers may be needed to increase sensitivity and specificity, this is of particular importance in populations with sub-microscopic infections or in whom other inflammatory diseases are prevalent

Interleukin-10 and soluble tumor necrosis factor receptor II are potential biomarkers of Plasmodium falciparum infections in pregnant women: a case-control study from Nanoro, Burkina Faso

Background: Diagnosis of malaria in pregnancy is problematic due to the low sensitivity of conventional diagnostic tests (rapid diagnostic test and microscopy), which is exacerbated due to low peripheral parasite densities, and lack of clinical symptoms. In this study, six potential biomarkers to support malaria diagnosis in pregnancy were evaluated.Methods: Blood samples were collected from pregnant women at antenatal clinic visits and at delivery. Microscopy and real-time PCR were performed for malaria diagnosis and biomarker analyses were performed by ELISA (interleukin 10, IL-10; tumor necrosis factor-α, TNF-α; soluble tumor necrosis factor receptor II, sTNF-RII; soluble fms-like tyrosine kinase 1, sFlt-1; leptin and apolipoprotein B, Apo-B). A placental biopsy was collected at delivery to determine placental malaria.Results: IL-10 and sTNF-RII were significantly higher at all time-points in malaria-infected women (p < 0.001). Both markers were also positively associated with parasite density (p < 0.001 and p = 0.003 for IL-10 and sTNF-RII respectively). IL-10 levels at delivery, but not during pregnancy, were negatively associated with birth weight. A prediction model was created using IL-10 and sTNF-RII cut-off points. For primigravidae the model had a sensitivity of 88.9% (95%CI 45.7–98.7%) and specificity of 83.3% (95% CI 57.1–94.9%) for diagnosing malaria during pregnancy. For secundi- and multigravidae the sensitivity (81.8% and 56.5% respectively) was lower, while specificity (100.0% and 94.3% respectively) was relatively high. Sub-microscopic infections were detected in 2 out of 3 secundi- and 5 out of 12 multigravidae.Conclusions: The combination of biomarkers IL-10 and sTNF-RII have the potential to support malaria diagnosis in pregnancy. Additional markers may be needed to increase sensitivity and specificity, this is of particular importance in populations with sub-microscopic infections or in whom other inflammatory diseases are prevalent

Ecological challenges for the buffer zone management of protected areas of forest-savannah mosaic in West Africa

In sub-Saharan Africa, the management of buffer zones around protected areas does not often take into serious account the needs of resource exploitation by the local populations or the conservation needs of these areas. We described the ecological characteristics and management issues affecting the buffer zone around the Fazao-Malfakassa National Park; a 192,000-ha protected area in central-western Togo of utmost conservation importance within the Dahomey Gap region. Within the buffer zone (10 km radius, 334,800 ha), we focussed on four high conservation value areas totalling 65,594 ha (20% of the total buffer zone area). Using 2015 sentinel-2 images we analyzed land cover patterns and described existing ecological zones. We complemented these with field surveys and interviews with 300 people living in 22 villages within the buffer zone to describe the conditions affecting the resident human population. Although over 60% of the total buffer zone area is degraded, we identified four areas of high conservation value (total area = 65,594 ha). Interviewees recognized that slash-and-burn was the most common form of land use, followed by agroforestry practices. Agriculture, charcoal, and firewood production were the main drivers affecting habitats, and land conflicts were recurrent due to the rise in human population. The decline in agriculture, reported by interviewees in some sectors, was attributable to ravages of crops by elephants. Three independent diversity indices showed that, in preserved zones, a greater diversity of animals (with similar utilization frequencies) were hunted than in degraded sites (where grasscutters were the dominant hunted species). There were also significant differences between degraded and preserved zones in terms of plants used for charcoal production and for non-timber forest products. We advocate the development of community-controlled hunting areas to enhance the conservation value of the four well-preserved zones. Instead, promoting sustainable agricultural production systems in the degraded areas can help to further stabilize the agricultural front and reduce land pressure on the park

Diagnosis of Bacterial Bloodstream Infections: A 16S Metagenomics Approach

YesBackground. Bacterial bloodstream infection (bBSI) is one of the leading causes of death in critically ill patients and accurate diagnosis is therefore crucial. We here report a 16S metagenomics approach for diagnosing and understanding bBSI. Methodology/Principal Findings. The proof-of-concept was delivered in 75 children (median age 15 months) with severe febrile illness in Burkina Faso. Standard blood culture and malaria testing were conducted at the time of hospital admission. 16S metagenomics testing was done retrospectively and in duplicate on the blood of all patients. Total DNA was extracted from the blood and the V3–V4 regions of the bacterial 16S rRNA genes were amplified by PCR and deep sequenced on an Illumina MiSeq sequencer. Paired reads were curated, taxonomically labeled, and filtered. Blood culture diagnosed bBSI in 12 patients, but this number increased to 22 patients when combining blood culture and 16S metagenomics results. In addition to superior sensitivity compared to standard blood culture, 16S metagenomics revealed important novel insights into the nature of bBSI. Patients with acute malaria or recovering from malaria had a 7-fold higher risk of presenting polymicrobial bloodstream infections compared to patients with no recent malaria diagnosis (p-value = 0.046). Malaria is known to affect epithelial gut function and may thus facilitate bacterial translocation from the intestinal lumen to the blood. Importantly, patients with such polymicrobial blood infections showed a 9-fold higher risk factor for not surviving their febrile illness (p-value = 0.030). Conclusions/Significance. Our data demonstrate that 16S metagenomics is a powerful approach for the diagnosis and understanding of bBSI. This proof-of-concept study also showed that appropriate control samples are crucial to detect background signals due to environmental contamination.This work was supported by the Flemish Ministry of Sciences (EWI, SOFI project IDIS).This paper has been subject to a correction. Please see Correction file above

A genomic appraisal of invasive Salmonella Typhimurium and associated antibiotic resistance in sub-Saharan Africa

Invasive non-typhoidal Salmonella (iNTS) disease manifesting as bloodstream infection with high mortality is responsible for a huge public health burden in sub-Saharan Africa. Salmonella enterica serovar Typhimurium (S. Typhimurium) is the main cause of iNTS disease in Africa. By analysing whole genome sequence data from 1303 S. Typhimurium isolates originating from 19 African countries and isolated between 1979 and 2017, here we show a thorough scaled appraisal of the population structure of iNTS disease caused by S. Typhimurium across many of Africa’s most impacted countries. At least six invasive S. Typhimurium clades have already emerged, with ST313 lineage 2 or ST313-L2 driving the current pandemic. ST313-L2 likely emerged in the Democratic Republic of Congo around 1980 and further spread in the mid 1990s. We observed plasmid-borne as well as chromosomally encoded fluoroquinolone resistance underlying emergences of extensive-drug and pan-drug resistance. Our work provides an overview of the evolution of invasive S. Typhimurium disease, and can be exploited to target control measures

Crop rotation and soil amendments: impacts on cotton and maize production in a cotton-based system in western Burkina Faso.

Crop production in the semi arid zone is limited by nutrients availability. Farmers in the area are unable to afford chemical fertilizers because of their low financial conditions. The design of cropping system with low inputs is therefore of importance for farmers. A crop rotation and soil amendment techniques study was carried out from 2000 to 2005 in Farakô-Ba research centre, western Burkina Faso to observe effects on maize and cotton seed yields. The treatments included six crop rotation systems and four soil amendments. The results showed a significant impact of crop rotation on both maize grain and cotton seed yields when a legume was included in the rotation. The yields were low when the rotation included only cereals and cotton. Soil amendment techniques including farmyard manure and phosphate rock generated the highest impact on crops yields while they were lowest when the straight fertilizers (urea, KCl, phosphate rock) were used. No significant interactions were found between crop rotation and soil amendment techniques. This suggests the possibility to save on fertilizers for maize while a minimum input is essential to support good cotton seed production. A crop rotation including a legume combined with organic manures is suitable to ensure a sustainable crop production in the semi arid zone of Burkina Faso. International Journal of Biological & Chemical Sciences Vol. 1 (2) 2007: pp. 143-15