5 research outputs found
Spatio-Temporal Dynamics of Yeast Mitochondrial Biogenesis: Transcriptional and Post-Transcriptional mRNA Oscillatory Modules
Examples of metabolic rhythms have recently emerged from studies of budding
yeast. High density microarray analyses have produced a remarkably detailed
picture of cycling gene expression that could be clustered according to
metabolic functions. We developed a model-based approach for the decomposition
of expression to analyze these data and to identify functional modules which,
expressed sequentially and periodically, contribute to the complex and intricate
mitochondrial architecture. This approach revealed that mitochondrial
spatio-temporal modules are expressed during periodic spikes and specific
cellular localizations, which cover the entire oscillatory period. For instance,
assembly factors (32 genes) and translation regulators (47 genes) are expressed
earlier than the components of the amino-acid synthesis pathways (31 genes). In
addition, we could correlate the expression modules identified with particular
post-transcriptional properties. Thus, mRNAs of modules expressed
“early” are mostly translated in the vicinity of
mitochondria under the control of the Puf3p mRNA-binding protein. This last
spatio-temporal module concerns mostly mRNAs coding for basic elements of
mitochondrial construction: assembly and regulatory factors. Prediction that
unknown genes from this module code for important elements of mitochondrial
biogenesis is supported by experimental evidence. More generally, these
observations underscore the importance of post-transcriptional processes in
mitochondrial biogenesis, highlighting close connections between nuclear
transcription and cytoplasmic site-specific translation
Integrative epigenomic mapping defines four main chromatin states in Arabidopsis
This first comprehensive view of the Arabidopsis epigenome reveals that it is organized into four main chromatin types based on the integrative mapping of a broad set of 11 histone marks and DNA methylation in seedlings
No viral transcripts associated with folliculotropic mycosis fungoides using a high throughput sequencing approach
Abstract is missing (Short communication