Genetic polymorphism of candidate genes in pig meat production

H-FABP, LEPR and MC5R genes were suggested as candidate genes for fat content in pig meat. The aim of this study was to detect genetic variation in the porcine H-FABP, LEPR and MC5R genes by PCR-RFLP method in a group of pigs. Genotyping of pigs was done by PCRRFLP methods. We identified three genotypes in the set of pigs, HH (0.504), Hh (0.412) and hh (0.084) for H-FABP (HinfI). Allele H showed higher frequency than allele h (0.710 vs. 0.290). Three genotypes were identified for the H-FABP (HaeIII) gene (DD - 0.194, Dd - 0.494, dd - 0.312). The allele D (0.441) showed slightly lower frequency than allele d (0.559). All three genotypes were identified for LEPR (HpaII) in the group of pigs (AA – 0.137, AB - 0.314, BB – 0.549). Higher frequency of LEPR gene was confirmed for allele B (0.706), as compared with allele A (0.294). We identified two genotypes for MC5R (BsaHI) in the group of pigs (AA - 0.348 and AG - 0.652), genotype GG was not found. As conforms with genotype structure, we recognize a higher frequency of allele A (0.674) as compared with allele G (0.326).&nbsp

Tick-borne encephalitis virus inhibits rRNA synthesis and host protein production in human cells of neural origin

Tick-borne encephalitis virus (TBEV), a member of the genus Flavivirus (Flaviviridae), is a causative agent of a severe neuroinfection. Recently, several flaviviruses have been shown to interact with host protein synthesis. In order to determine whether TBEV interacts with this host process in its natural target cells, we analysed de novo protein synthesis in a human cell line derived from cerebellar medulloblastoma (DAOY HTB-186). We observed a significant decrease in the rate of host protein synthesis, including the housekeeping genes HPRT1 and GAPDH and the known interferon-stimulated gene viperin. In addition, TBEV infection resulted in a specific decrease of RNA polymerase I (POLR1) transcripts, 18S and 28S rRNAs and their precursor, 45-47S pre-rRNA, but had no effect on the POLR3 transcribed 5S rRNA levels. To our knowledge, this is the first report of flavivirus-induced decrease of specifically POLR1 rRNA transcripts accompanied by host translational shut-off

Production of Electric Energy by Use of Wind Energy

Taking advantage of the wind is one of the oldest ways to acquire energy. Traditional wind power sources are classic windmills. Nowadays, we are trying to focus more on generating electricity from alternative sources and one of these sources is also the energy of the wind. The most often uses of the potential of wind power is to generate electricity. In addition, this energy is attractive today because its use does not produce any waste, does not pollute the air and does not have a negative impact on human health. Recently, we can recognize using of so-called "small wind power plants” in households. This paper provides an overview and technological solutions to be taken into account for designing and realizing the construction of a wind power plant. This paper proposed rotor with a horizontal axis which bears the designation of Savoni rotor. This paper represents a contribution to the development of the use of small wind power plants, as contains a large number of illustration

Tick-borne viruses and biological processes at the tick-host-virus interface

Ticks are efficient vectors of arboviruses, although less than 10% of tick species are known to be virus vectors. Most tick-borne viruses (TBV) are RNA viruses some of which cause serious diseases in humans and animals world-wide. Several TBV impacting human or domesticated animal health have been found to emerge or re-emerge recently. In order to survive in nature, TBV must infect and replicate in both vertebrate and tick cells, representing very different physiological environments. Information on molecular mechanisms that allow TBV to switch between infecting and replicating in tick and vertebrate cells is scarce. In general, ticks succeed in completing their blood meal thanks to a plethora of biologically active molecules in their saliva that counteract and modulate different arms of the host defense responses (haemostasis, inflammation, innate and acquired immunity, and wound healing). The transmission of TBV occurs primarily during tick feeding and is a complex process, known to be promoted by tick saliva constituents. However, the underlying molecular mechanisms of TBV transmission are poorly understood. Immunomodulatory properties of tick saliva helping overcome the first line of defense to injury and early interactions at the tick-host skin interface appear to be essential in successful TBV transmission and infection of susceptible vertebrate hosts. The local host skin site of tick attachment, modulated by tick saliva, is an important focus of virus replication. Immunomodulation of the tick attachment site also promotes co-feeding transmission of viruses from infected to non-infected ticks in the absence of host viraemia (non-viraemic transmission). Future research should be aimed at identification of the key tick salivary molecules promoting virus transmission, and a molecular description of tick-host-virus interactions and of tick-mediated skin immunomodulation. Such insights will enable the rationale design of anti-tick vaccines that protect against disease caused by tick-borne viruses

The life of a patient with the illness disease Ankylosing

Tématem mé bakalářské práce je Život pacienta s onemocněním Bechtěrevova choroba. Práce je rozdělena do dvou částí. První je teoretická část, zde je pojednáváno o anatomii páteře, zejména křížové oblasti. Část práce je věnovaná samotnému onemocnění Bechtěrevova choroba, základnímu rozdělení, ztuhnutí páteře klinickému obrazu a léčbě. Jedna z kapitol se věnuje vyšetření hybnosti páteře. V oblasti léčby jsem se nejvíce zaměřila na pohybovou aktivitu. Druhá část je výzkumná, zaměřená na vyhodnocení dotazníku a statistické zpracování údajů u vybraných respondentů ve zdravotnickém zařízení provádějící diagnostiku a ambulantní a hospitalizační léčbu revmatických onemocnění. Dotazníky byly zaměřeny především na kvalitu života, jak ambulantních, tak hospitalizovaných klientů. Především jsem zjišťovala, jak se cítí, zda je nemoc nějak omezuje. Praktickým výstupem práce je návrh cvičebných úkonů, které brání ztuhnutí páteře a ve výsledku působí i úlevu od bolesti. Účelem je protažení páteře a velkých kloubů.The topic of my thesis is "the patient´s life with the Bechterew disease". The work is divided into two parts. The first part is theoretical; here is the analysis of the anatomy of the spine, especially the lumbar. Part of the work is devoted to the Bechterew's disease, primary distribution, stiff spine clinical picture and treatment. One chapter is devoted to examination of spinal mobility. In the area of treatment I was most focused on physical activity. The second part of my thesis is research oriented, focused on the evaluation of the questionnaire and statistical processing of data from selected respondents in a health care facility performing diagnostics and outpatient and inpatient treatment of rheumatic diseases. Questionnaires were focused primarily on the quality of life of both outpatient and hospitalized clients. In particular, I investigated how they feel, whether the disease somehow restricts them. The practical outcome of this work is to design exercise acts that prevent stiffening of the spine and result in operating and relief from pain. The purpose is to stretch the spine and large joints.Katedra ošetřovatelstvíHodnocení vedoucího: výborně mínus Hodnocení oponenta: výborně mínus Doplňující otázky k obhajobě: 1. Jak pacienti ve sledované skupině tiší svou bolest? 2. Využívají pacienti ve sledované skupině nefarmakologické léčby bolesti, a jaké? Obhajoba bakalářské práce s prezentací velmi dobrá