187 research outputs found
Lattice Boltzmann simulations of anisotropic particles at liquid interfaces
Complex colloidal fluids, such as emulsions stabilized by complex shaped
particles, play an important role in many industrial applications. However,
understanding their physics requires a study at sufficiently large length
scales while still resolving the microscopic structure of a large number of
particles and of the local hydrodynamics. Due to its high degree of locality,
the lattice Boltzmann method, when combined with a molecular dynamics solver
and parallelized on modern supercomputers, provides a tool that allows such
studies. Still, running simulations on hundreds of thousands of cores is not
trivial. We report on our practical experiences when employing large fractions
of an IBM Blue Gene/P system for our simulations. Then, we extend our model for
spherical particles in multicomponent flows to anisotropic ellipsoidal objects
rendering the shape of e.g. clay particles. The model is applied to a number of
test cases including the adsorption of single particles at fluid interfaces and
the formation and stabilization of Pickering emulsions or bijels.Comment: 10 pages, 5 figures; ParCFD 2011 proceedings contributio
Zoonosis emergence linked to agricultural intensification and environmental change
A systematic review was conducted by a multidisciplinary team to analyze qualitatively best available scientific evidence on the effect of agricultural intensification and environmental changes on the risk of zoonoses for which there are epidemiological interactions between wildlife and livestock. The study found several examples in which agricultural intensification and/or environmental change were associated with an increased risk of zoonotic disease emergence, driven by the impact of an expanding human population and changing human behavior on the environment. We conclude that the rate of future zoonotic disease emergence or reemergence will be closely linked to the evolution of the agriculture–environment nexus. However, available research inadequately addresses the complexity and interrelatedness of environmental, biological, economic, and social dimensions of zoonotic pathogen emergence, which significantly limits our ability to predict, prevent, and respond to zoonotic disease emergence
Free energy of colloidal particles at the surface of sessile drops
The influence of finite system size on the free energy of a spherical
particle floating at the surface of a sessile droplet is studied both
analytically and numerically. In the special case that the contact angle at the
substrate equals a capillary analogue of the method of images is
applied in order to calculate small deformations of the droplet shape if an
external force is applied to the particle. The type of boundary conditions for
the droplet shape at the substrate determines the sign of the capillary
monopole associated with the image particle. Therefore, the free energy of the
particle, which is proportional to the interaction energy of the original
particle with its image, can be of either sign, too. The analytic solutions,
given by the Green's function of the capillary equation, are constructed such
that the condition of the forces acting on the droplet being balanced and of
the volume constraint are fulfilled. Besides the known phenomena of attraction
of a particle to a free contact line and repulsion from a pinned one, we
observe a local free energy minimum for the particle being located at the drop
apex or at an intermediate angle, respectively. This peculiarity can be traced
back to a non-monotonic behavior of the Green's function, which reflects the
interplay between the deformations of the droplet shape and the volume
constraint.Comment: 24 pages, 19 figure
Electron-Electron Interaction in Disordered Mesoscopic Systems: Weak Localization and Mesoscopic Fluctuations of Polarizability and Capacitance
The weak localization correction and the mesoscopic fluctuations of the
polarizability and the capacitance of a small disordered sample are studied
systematically in 2D and 3D geometries. While the grand canonical ensemble
calculation gives the positive magnetopolarizability, in the canonical ensemble
(appropriate for isolated samples) the sign of the effect is reversed. The
magnitude of mesoscopic fluctuations for a single sample exceeds considerably
the value of the weak localization correction.Comment: 13 pages Latex, 3 .eps figures included. To appear in Phys. Rev. B.
Minor corrections, in particular in formulae; new references adde
Identification of O-mannosylated Virulence Factors in Ustilago maydis
The O-mannosyltransferase Pmt4 has emerged as crucial for fungal virulence in the animal pathogens Candida albicans or Cryptococcus neoformans as well as in the phytopathogenic fungus Ustilago maydis. Pmt4 O-mannosylates specific target proteins at the Endoplasmic Reticulum. Therefore a deficient O-mannosylation of these target proteins must be responsible for the loss of pathogenicity in pmt4 mutants. Taking advantage of the characteristics described for Pmt4 substrates in Saccharomyces cerevisiae, we performed a proteome-wide bioinformatic approach to identify putative Pmt4 targets in the corn smut fungus U. maydis and validated Pmt4-mediated glycosylation of candidate proteins by electrophoretic mobility shift assays. We found that the signalling mucin Msb2, which regulates appressorium differentiation upstream of the pathogenicity-related MAP kinase cascade, is O-mannosylated by Pmt4. The epistatic relationship of pmt4 and msb2 showed that both are likely to act in the same pathway. Furthermore, constitutive activation of the MAP kinase cascade restored appressorium development in pmt4 mutants, suggesting that during the initial phase of infection the failure to O-mannosylate Msb2 is responsible for the virulence defect of pmt4 mutants. On the other hand we demonstrate that during later stages of pathogenic development Pmt4 affects virulence independently of Msb2, probably by modifying secreted effector proteins. Pit1, a protein required for fungal spreading inside the infected leaf, was also identified as a Pmt4 target. Thus, O-mannosylation of different target proteins affects various stages of pathogenic development in U. maydis
РЕЦЕПТОР ЧЕЛОВЕЧЕСКОГО ЭПИДЕРМАЛЬНОГО ФАКТОРА РОСТА HER2 ПРИ РАКЕ ЖЕЛУДКА (РЕЗУЛЬТАТЫ МНОГОЦЕНТРОВОГО КЛИНИЧЕСКОГО ИССЛЕДОВАНИЯ)
In this article results of the multicenter trial are displayed. Data of successful efficacy of trastuzumab treatment in HER2 positive gastric cancer patients has shown.В статье приведены данные многоцентрового исследования. Показаны успешные результаты лечения трастузумабом больных с HER2 позитивным раком желудка
Membrane-mediated interactions
Interactions mediated by the cell membrane between inclusions, such as
membrane proteins or antimicrobial peptides, play important roles in their
biological activity. They also constitute a fascinating challenge for
physicists, since they test the boundaries of our understanding of
self-assembled lipid membranes, which are remarkable examples of
two-dimensional complex fluids. Inclusions can couple to various degrees of
freedom of the membrane, resulting in different types of interactions. In this
chapter, we review the membrane-mediated interactions that arise from direct
constraints imposed by inclusions on the shape of the membrane. These effects
are generic and do not depend on specific chemical interactions. Hence, they
can be studied using coarse-grained soft matter descriptions. We deal with
long-range membrane-mediated interactions due to the constraints imposed by
inclusions on membrane curvature and on its fluctuations. We also discuss the
shorter-range interactions that arise from the constraints on membrane
thickness imposed by inclusions presenting a hydrophobic mismatch with the
membrane.Comment: 38 pages, 10 figures, pre-submission version. In: Bassereau P., Sens
P. (eds) Physics of Biological Membranes. Springer, Cha
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Effect of NaCl on the Growth of Germinating Upland Cotton (Gossypium hirsutum L.) Seeds
Responses to environmental stresses such as excess salinity are difficult to understand if not studied under defined conditions. The objective of this study was to determine if cottonseed radicle and cotyledon growth during germination was affected by exogenous NaCl. Cottonseeds were imbibed in moistened paper toweling for 24 h at 32°C before transfer to an agar media containing selected concentrations of NaCl for an additional 24 h. Radicle linear growth extension was stimulated at low NaC1 concentrations (80 mM), but increasingly inhibited as NaCl concentrations increased from 160 to 400 mM. Radicle and cotyledon fresh weights were increasingly inhibited by NaCl concentrations between 0 and 400 mM. NaCl inhibition of radicle fresh weights was more pronounced than that of cotyledons. We conclude that the inhibitory effects of NaCl can be quantified as reductions in cottonseed radicle and cotyledon growth
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