Development of a Questionnaire for the Search for Occupational Causes in Patients with Non-Hodgkin Lymphoma: The RHELYPRO Study

Non-Hodgkin lymphoma (NHL), multiple myeloma and chronic lymphocytic leukemia are possibly related to environmental and/or occupational exposure. The primary objective of this study was to develop a questionnaire for screening patients with these blood disorders who might benefit from a specialized consultation for possible recognition of the disease as an occupational disease. The study included 205 subjects (male gender, 67.3%; mean age, 60 years; NHL, 78.5%). The questionnaire performed very satisfactorily in identifying the exposures most frequently retained by experts for their potential involvement in the occurrence of NHL. Its sensitivity and specificity in relation to the final expertise were 96% and 96% for trichloroethylene, 85% and 82% for benzene, 78% and 87% for solvents other than trichloroethylene and dichloromethane, 87% and 95% for pesticides, respectively. Overall, 15% of the subjects were invited to ask National Social Insurance for compensation as occupational disease. These declarations concerned exposure to pesticides (64%), solvents (trichloroethylene: 29%; benzene: 18%; other than chlorinated solvents: 18%) and sometimes multiple exposures. In conclusion, this questionnaire appears as a useful tool to identify NHL patients for a specialized consultation, in order to ask for compensation for occupational disease

Brexucabtagene autoleucel in relapsed or refractory mantle cell lymphoma, intention-to-treat use in the DESCAR-T registry

Not available

TRAF4 is a novel phosphoinositide-binding protein modulating tight junctions and favoring cell migration

Tumor necrosis factor (TNF) receptor-associated factor 4 (TRAF4) is frequently overexpressed in carcinomas, suggesting a specific role in cancer. Although TRAF4 protein is predominantly found at tight junctions (TJs) in normal mammary epithelial cells (MECs), it accumulates in the cytoplasm of malignant MECs. How TRAF4 is recruited and functions at TJs is unclear. Here we show that TRAF4 possesses a novel phosphoinositide (PIP)-binding domain crucial for its recruitment to TJs. Of interest, this property is shared by the other members of the TRAF protein family. Indeed, the TRAF domain of all TRAF proteins (TRAF1 to TRAF6) is a bona fide PIP-binding domain. Molecular and structural analyses revealed that the TRAF domain of TRAF4 exists as a trimer that binds up to three lipids using basic residues exposed at its surface. Cellular studies indicated that TRAF4 acts as a negative regulator of TJ and increases cell migration. These functions are dependent from its ability to interact with PIPs. Our results suggest that TRAF4 overexpression might contribute to breast cancer progression by destabilizing TJs and favoring cell migration

Exploring the richness of the French Galaxy Ecosystem

International audienceThe French Bioinformatics Community has embraced Galaxy since its inception, with a pivotal moment being the Galaxy Tour de France led by Nate Coroar, Anton Nekrutenko, and James Taylor in 2012. This adoption has led to the establishment of over 10 Galaxy servers across France, catering to diverse local needs and specialized thematic areas such as ecology, biodiversity, NGS, proteomics, and more.Among these servers, UseGalaxy.fr stands out as the flagship national instance, launched in 2021 and hosted by the French Institute for Bioinformatics (IFB - ELIXIR-FR). With robust infrastructure boasting 8300 CPU cores, 52 TB of RAM, and GPU cards, UseGalaxy.fr offers a comprehensive suite of over 3,000 tools, including interactive options like Jupyter Notebook, AlphaFold, and Helixer. Notably, it has garnered over 6,000 users who have collectively executed over 3.6 million jobs. Moreover, UseGalaxy.fr hosts specialized subdomains catering to various community needs, such as ecology, metabarcoding, and COVID-19 research, with ongoing integration of new subdomains.The community's commitment to collaboration and consolidation is evident as several local servers have migrated to UseGalaxy.fr in recent years, with others expressing interest in doing the same. The French Galaxy community is deeply engaged in a multitude of projects at national, European, and global levels, including EOSC FAIR EASE, EuroScienceGateway, ATLASea and ABRomics.To foster cohesion and synergy within the community, a Galaxy Working Group led by the French Bioinformatics Institute facilitates regular interactions. This group serves to connect Galaxy users across France, share knowledge, support UseGalaxy.fr, and combat misconceptions about Galaxy within the French scientific community.In this poster presentation, we provide an overview of the dynamic French Galaxy ecosystem, highlighting its diverse servers, engaged researchers, ongoing projects, and collaborative efforts. Through this exploration, we aim to showcase the vibrancy and impact of Galaxy within the French bioinformatics landscape

BeEAM (bendamustine, etoposide, cytarabine, melphalan) prior to autologous stem cell transplant for chemosensitive relapses in patients with follicular lymphoma: a prospective multicentre phase II study in Lymphoma Study Association centres

International audienceNo abstract availabl

Serological responses against seasonal influenza viruses in patients with multiple myeloma treated or untreated with daratumumab after two doses of tetravalent vaccine

International audienceObjectivesDaratumumab-treated myeloma patients may face increased seasonal influenza risk due to weakened postvaccination immune responses, especially with daratumumab treatment. We aimed to assess humoral responses to boosted influenza vaccination in daratumumab-treated or -untreated patients.MethodsIn a single-center study, we evaluated humoral responses (hemagglutination-inhibition assay) one month following a two-injection (4-weeks apart) influenza vaccination (standard dose) in 84 patients with multiple myeloma (40 with daratumumab in the past year).ResultsSeroprotection rates (titer ≥1/40) after the second vaccine injection were low across vaccinal subtypes (except for A-H3N2): 71.3% (A-H3N2), 19.7% (A-H1N1pdm09), 9.9% (B-Victoria), 11.3% (B-Yamagata). Only A-H3N2 seroprotection rates significantly increased with the booster in daratumumab-treated patients (30% (12/40) after one injection vs 55% (22/40) after the boost; P = 0.01).After propensity score weighting, daratumumab was not significantly associated with a reduced likelihood of seroprotection against at least one vaccine strain (OR 0.65 [95% CI: 0.22-1.88]).ConclusionWhile daratumumab treatment did not lead to a significant reduction in seroprotection rates following influenza vaccination, a booster vaccine injection demonstrated potential benefit for specific strains (A-H3N2) in patients undergoing daratumumab treatment. Nevertheless, the overall low response rates in patients with multiple myeloma necessitates the development of alternative vaccination and prophylaxis strategies

Mouvements paysans face à la politique agricole commune et à la mondialisation (1957-2011)

Cette publication s'inscrit dans le cadre du programme de recherche du Centre de Recherches en Histoire Internationale et Atlantique de l'université de Nantes sur l'Histoire des mouvements sociaux dans l'Europe Atlantique. Réfléchissant aux réactions des sociétés civiles de l'ère contemporaine face aux grands événements internationaux de leur temps, ce programme, mené en lien avec les ressources du Centre d'Histoire du Travail (CHT), s'est intéressé à l'histoire des mouvements paysans face aux mutations agricoles conditionnées par la création de la Politique Agricole Commune et face aux changements induits par le développement de l'Organisation Mondiale du Commerce. Outre des mises en perspective larges sur les problématiques de la PAC et de l'OMC, cet ouvrage collectif, issu d'une journée d'études, a cherché à approfondir certains conflits sociaux par la publication d'études scientifiques et monographiques portant sur les régions de l'Ouest, du Sud-Ouest et du monde méditerranéen, de témoignages ou de documents d'archives venant des organisations agricoles (le plan Mansholt, les grèves du lait, les conflits viticoles, les conflits de l'agroalimentaire…)

Scientific workflows for computational reproducibility in the life sciences: Status, challenges and opportunities

International audienceWith the development of new experimental technologies, biologists are faced with an avalanche of data to be computationally analyzed for scientific advancements and discoveries to emerge. Faced with the complexity of analysis pipelines, the large number of computational tools, and the enormous amount of data to manage, there is compelling evidence that many if not most scientific discoveries will not stand the test of time: increasing the reproducibility of computed results is of paramount importance. The objective we set out in this paper is to place scientific workflows in the context of reproducibility. To do so, we define several kinds of repro-ducibility that can be reached when scientific workflows are used to perform experiments. We characterize and define the criteria that need to be catered for by reproducibility-friendly scientific workflow systems, and use such criteria to place several representative and widely used workflow systems and companion tools within such a framework. We also discuss the remaining challenges posed by reproducible scientific workflows in the life sciences. Our study was guided by three use cases from the life science domain involving in silico experiments

Echocardiographic Patterns of Left Ventricular Diastolic Function in Cardiac Amyloidosis: An Updated Evaluation

Aims: Multimodal imaging has allowed cardiac amyloidosis (CA) to be increasingly recognised as a treatable cause of heart failure with preserved ejection fraction, but its prognosis remains poor due to late diagnosis. To assess the left ventricular diastolic function (LVDF) patterns in a large contemporary CA cohort according to the current recommendations and to identify their determinants. Methods and Results: We conducted a monocentric, observational study on a cohort of CA patients from a tertiary CA referral centre. Diastolic function was analysed using standard echocardiography and clinical, laboratory and survival parameters were collected. Four hundred and sixty-four patients with one of the three main type of CA were included: 41% had grade III diastolic dysfunction (restrictive mitral pattern), 25% had grade II diastolic dysfunction, and 25% had grade I diastolic dysfunction; 9% were unclassified. No difference was found between the main CA types. After multivariate analyses, grades II and III were independently associated with dyspnoea, elevated NT-proBNP, cardiac infiltration and systolic dysfunction (global longitudinal strain). Grade I patients had a better prognosis. Conclusions: All LVDF patterns can be observed in CA. One quarter of CA patients have grade I LVDF, reflecting the emergence of earlier stage-related phenotypes with a better prognosis