Análise de dados aplicada à enogastronomia

Orientador : Denise Fukumi TsunodaMonografia (graduação) - Universidade Federal do Paraná, Setor de Ciências Sociais Aplicadas, Curso de Graduação em Gestão da Informação.Inclui referênciasResumo : Estudo de natureza qualitativa que objetiva realizar uma análise de dados a partir de técnicas estatísticas e mineração de dados, a fim de identificar relações e padrões de ocorrência em uma base de dados Enogastronômica. Visa a concepção de uma base de dados que reúna elementos da Gastronomia e Enologia delimitando-se a pizzarias de Curitiba premiadas nos concursos Bom Gourmet 2018 e Comer&Beber 2017/2018, cuja finalidade é comparar os resultados de 2 (dois) algoritmos de mineração de dados, Apriori e Prism, voltados para a descoberta de padrões a partir de regras de associação e classificação; e, aplicar 2 (dois) métodos estatísticos, Qui-quadrado e Análise de Correspondência Múltipla (ACM), com o propósito de realizar uma análise descritiva da base de dados. Apresenta resultados satisfatórios na execução dos algoritmos de mineração de dados, em que a aplicação do Apriori resulta em regras com grau de interesse (lift) superior a 1,45, apresentando uma correlação positiva entre os atributos, além de compor regras com dois ou mais atributos no consequente; enquanto para o Prism considerou os atributos que possuem uma taxa de acerto inicial de 85,00% entre as instâncias, onde o maior índice de acerto foi na aplicação cujo atributo-meta é o ingrediente molho de tomate com 96,89% .Valida as regras geradas nestas aplicações junto aos especialistas com a finalidade de verificar a ocorrência destas no ato de uma harmonização ou a geração de um novo conhecimento. Revela que na inferência estatística, o teste Qui-quadrado apresenta diferenças estatisticamente significativas em um dos atributos e no ACM é possível explicar o método em 2 (duas) dimensões e visualizar 4 (quatro) agrupamentos distintos. Verifica a existência de relação entre um agrupamento da ACM e as regras do algoritmo Prism. Sugere como trabalhos futuros, a ampliação da base de dados para a realização de uma análise qualitativa dos dados; a concepção de outras bases de dados Enogastronômicas voltadas a demais estilos de Gastronomia; e, a criação de um guia de harmonização a partir dos dados coletados, visando auxiliar a escolha do cliente dos estabelecimentos participantes do estudo

Converting Speech to Text Using JAVAscript

This article is about the process of working with the Web Speech API. This is a very powerful browser interface that allows to record human speech and convert it to text. We will also consider the way to use it for the opposite procedure of reading lines with a human voice

Effective Methods of Seo Promotion of Web Sites Built on the Basis of Cms Wordpress

The article presents practical ways of work with web sites built on the basis of CMS Wordpress. They will help to achieve maximum results in their SEO promotion. It is considered as a built-in CMS Wordpress functionality, presented by third-party plug-ins

Maintaining a sense of direction during long-range communication on DNA

Many biological processes rely on the interaction of proteins with multiple DNA sites separated by thousands of base pairs. These long-range communication events can be driven by both the thermal motions of proteins and DNA, and directional protein motions that are rectified by ATP hydrolysis. The present review describes conflicting experiments that have sought to explain how the ATP-dependent Type III restriction–modification enzymes can cut DNA with two sites in an inverted repeat, but not DNA with two sites in direct repeat. We suggest that an ATPase activity may not automatically indicate a DNA translocase, but can alternatively indicate a molecular switch that triggers communication by thermally driven DNA sliding. The generality of this mechanism to other ATP-dependent communication processes such as mismatch repair is also discussed

Characterization and crystal structure of the type IIG restriction endonuclease RM.BpuSI

A type IIG restriction endonuclease, RM.BpuSI from Bacillus pumilus, has been characterized and its X-ray crystal structure determined at 2.35Å resolution. The enzyme is comprised of an array of 5-folded domains that couple the enzyme's N-terminal endonuclease domain to its C-terminal target recognition and methylation activities. The REase domain contains a PD-x15-ExK motif, is closely superimposable against the FokI endonuclease domain, and coordinates a single metal ion. A helical bundle domain connects the endonuclease and methyltransferase (MTase) domains. The MTase domain is similar to the N6-adenine MTase M.TaqI, while the target recognition domain (TRD or specificity domain) resembles a truncated S subunit of Type I R–M system. A final structural domain, that may form additional DNA contacts, interrupts the TRD. DNA binding and cleavage must involve large movements of the endonuclease and TRD domains, that are probably tightly coordinated and coupled to target site methylation status

The fragment structure of a putative HsdR subunit of a type I restriction enzyme from Vibrio vulnificus YJ016: implications for DNA restriction and translocation activity

Among four types of bacterial restriction enzymes that cleave a foreign DNA depending on its methylation status, type I enzymes composed of three subunits are interesting because of their unique DNA cleavage and translocation mechanisms performed by the restriction subunit (HsdR). The elucidated N-terminal fragment structure of a putative HsdR subunit from Vibrio vulnificus YJ016 reveals three globular domains. The nucleolytic core within an N-terminal nuclease domain (NTD) is composed of one basic and three acidic residues, which include a metal-binding site. An ATP hydrolase (ATPase) site at the interface of two RecA-like domains (RDs) is located close to the probable DNA-binding site for translocation, which is far from the NTD nucleolytic core. Comparison of relative domain arrangements with other functionally related ATP and/or DNA complex structures suggests a possible translocation and restriction mechanism of the HsdR subunit. Furthermore, careful analysis of its sequence and structure implies that a linker helix connecting two RDs and an extended region within the nuclease domain may play a central role in switching the DNA translocation into the restriction activity

Type III restriction endonuclease EcoP15I is a heterotrimeric complex containing one Res subunit with several DNA-binding regions and ATPase activity

For efficient DNA cleavage, the Type III restriction endonuclease EcoP15I communicates with two inversely oriented recognition sites in an ATP-dependent process. EcoP15I consists of methylation (Mod) and restriction (Res) subunits forming a multifunctional enzyme complex able to methylate or to cleave DNA. In this study, we determined by different analytical methods that EcoP15I contains a single Res subunit in a Mod2Res stoichiometry. The Res subunit comprises a translocase (Tr) domain carrying functional motifs of superfamily 2 helicases and an endonuclease domain with a PD..D/EXK motif. We show that the isolated Tr domain retains ATP-hydrolyzing activity and binds single- and double-stranded DNA in a sequence-independent manner. To localize the regions of DNA binding, we screened peptide arrays representing the entire Res sequence for their ability to interact with DNA. We discovered four DNA-binding regions in the Tr domain and two DNA-binding regions in the endonuclease domain. Modelling of the Tr domain shows that these multiple DNA-binding regions are located on the surface, free to interact with DNA. Interestingly, the positions of the DNA-binding regions are conserved among other Type III restriction endonucleases

Type I restriction enzymes and their relatives

Type I restriction enzymes (REases) are large pentameric proteins with separate restriction (R), methylation (M) and DNA sequence-recognition (S) subunits. They were the first REases to be discovered and purified, but unlike the enormously useful Type II REases, they have yet to find a place in the enzymatic toolbox of molecular biologists. Type I enzymes have been difficult to characterize, but this is changing as genome analysis reveals their genes, and methylome analysis reveals their recognition sequences. Several Type I REases have been studied in detail and what has been learned about them invites greater attention. In this article, we discuss aspects of the biochemistry, biology and regulation of Type I REases, and of the mechanisms that bacteriophages and plasmids have evolved to evade them. Type I REases have a remarkable ability to change sequence specificity by domain shuffling and rearrangements. We summarize the classic experiments and observations that led to this discovery, and we discuss how this ability depends on the modular organizations of the enzymes and of their S subunits. Finally, we describe examples of Type II restriction–modification systems that have features in common with Type I enzymes, with emphasis on the varied Type IIG enzymes

Struktura motorové podjednotky a translokační model pro EcoR124I restrikční-modifikační komplex

structure determination of HsdR motor subunit and translocation model for EcoR124I restriction-modification comple