Interactions between metals and soil organic matter in various particle size fractions of soil contaminated with waste water

Only scarce field studies concern the consequences of natural soil organic matter (SOM) and metal interactions on SOM dynamics in soils. We investigated the interactions of four metals (Pb, Zn, Cu and Cd) with the SOM associated to five different size fractions (between 2000 μm and b2 μm) of a sandy top soil contaminated by waste water. Metal, organic carbon and nitrogen concentrations were measured and chemical extractions (with Na4P2O7 and EDTA) were also performed to assess the variations of SOM–metal interactions irrespective of the size fraction. In addition, as in that selected contaminated site, maize (C4 plant), replaced C3 crops 15 years ago, natural isotopic 13C labelling gave new insights into SOM turnover. First, the results suggest that metals influence the SOM dynamics in that sandy soil: a C3 "old carbon" enrichment was observed in the small or clay size fractions, while the "new" C4 carbon associated with sandy soil particles presents a rapid turnover. Metal accumulation in the clay fraction is attributed to particulate organic matter (poorly associated) and SOM decay which overtime accumulated metals and eventually these metal–SOM associations prevent the biological decomposition of such carbon pools. Moreover, the δ13C signals, C/N ratios and results from chemical extractions clearly showed differences in the origin, nature and reactivity of the SOM as a function of the size fraction with consequences on the metal behaviour. Differences were observed between metals studied: Zn seems to be mainly bound to SOM associated with clay particles, while Pb seems to prefer to interact directly with the mineral surfaces versus the SOM

Genomic Diversity of the Ostreid Herpesvirus Type 1 Across Time and Location and Among Host Species

The mechanisms underlying virus emergence are rarely well understood, making the appearance of outbreaks largely unpredictable. This is particularly true for pathogens with low per-site mutation rates, such as DNA viruses, that do not exhibit a large amount of evolutionary change among genetic sequences sampled at different time points. However, whole-genome sequencing can reveal the accumulation of novel genetic variation between samples, promising to render most, if not all, microbial pathogens measurably evolving and suitable for analytical techniques derived from population genetic theory. Here, we aim to assess the measurability of evolution on epidemiological time scales of the Ostreid herpesvirus 1 (OsHV-1), a double stranded DNA virus of which a new variant, OsHV-1 μVar, emerged in France in 2008, spreading across Europe and causing dramatic economic and ecological damage. We performed phylogenetic analyses of heterochronous (n = 21) OsHV-1 genomes sampled worldwide. Results show sufficient temporal signal in the viral sequences to proceed with phylogenetic molecular clock analyses and they indicate that the genetic diversity seen in these OsHV-1 isolates has arisen within the past three decades. OsHV-1 samples from France and New Zealand did not cluster together suggesting a spatial structuration of the viral populations. The genome-wide study of simple and complex polymorphisms shows that specific genomic regions are deleted in several isolates or accumulate a high number of substitutions. These contrasting and non-random patterns of polymorphism suggest that some genomic regions are affected by strong selective pressures. Interestingly, we also found variant genotypes within all infected individuals. Altogether, these results provide baseline evidence that whole genome sequencing could be used to study population dynamic processes of OsHV-1, and more broadly herpesviruses

Are animals a source of Stenotrophomonas maltophilia in human infections? Contributions of a nationwide molecular study

Stenotrophomonas maltophilia (Sm) is an archetypal environmental opportunistic bacterium responsible for health care-associated infections. The role of animals in human Sm infections is unknown. This study aims to reveal the genetic and phylogenetic relationships between pathogenic strains of Sm, both animal and human, and identify a putative role for animals as a reservoir in human infection. We phenotypically and genotypically characterized 61 Sm strains responsible for animal infections (mainly respiratory tract infections in horses) from a French nationwide veterinary laboratory network. We tested antimicrobial susceptibility and performed MLST and genogrouping using the concatenation of the seven housekeeping genes from the original MLST scheme. Excluding the eight untypeable strains owing to the lack of gene amplification, only 10 out of the 53 strains yielded a known ST (ST5, ST39, ST162, ST8, ST27, ST126, ST131). The genogroup distribution highlighted not only genogroups (genogroups 5 and 9) comprised exclusively of animal strains but also genogroups shared by human and animal strains. Interestingly, these shared genogroups were primarily groups 2 and 6, which have previously been identified as the two most frequent genogroups among human-pathogenic Sm strains, especially among respiratory pathogens. The antimicrobial susceptibility testing underlined the presence of acquired resistance: 18.8 and 7.5% of the tested isolates were resistant to the sulfonamide-trimethoprim combination and ciprofloxacin, respectively. Animal strains of Sm shared phylogenetic traits with some of the most successful human strains. The exact relationships between the human and animal strains, and the genetic support of these common traits, need to be determined

Design status of ASPIICS, an externally occulted coronagraph for PROBA-3

The "sonic region" of the Sun corona remains extremely difficult to observe with spatial resolution and sensitivity sufficient to understand the fine scale phenomena that govern the quiescent solar corona, as well as phenomena that lead to coronal mass ejections (CMEs), which influence space weather. Improvement on this front requires eclipse-like conditions over long observation times. The space-borne coronagraphs flown so far provided a continuous coverage of the external parts of the corona but their over-occulting system did not permit to analyse the part of the white-light corona where the main coronal mass is concentrated. The proposed PROBA-3 Coronagraph System, also known as ASPIICS (Association of Spacecraft for Polarimetric and Imaging Investigation of the Corona of the Sun), with its novel design, will be the first space coronagraph to cover the range of radial distances between ~1.08 and 3 solar radii where the magnetic field plays a crucial role in the coronal dynamics, thus providing continuous observational conditions very close to those during a total solar eclipse. PROBA-3 is first a mission devoted to the in-orbit demonstration of precise formation flying techniques and technologies for future European missions, which will fly ASPIICS as primary payload. The instrument is distributed over two satellites flying in formation (approx. 150m apart) to form a giant coronagraph capable of producing a nearly perfect eclipse allowing observing the sun corona closer to the rim than ever before. The coronagraph instrument is developed by a large European consortium including about 20 partners from 7 countries under the auspices of the European Space Agency. This paper is reviewing the recent improvements and design updates of the ASPIICS instrument as it is stepping into the detailed design phase

La perte de SCRIB enflamme les macrophages

Cette année encore, dans le cadre du module d’enseignement « Physiopathologie de la signalisation » proposé par l’université Paris-sud, les étudiants du Master « Biologie Santé » de l’université Paris-Saclay se sont confrontés à l’écriture scientifique. Ils ont sélectionné 12 articles scientifiques récents dans le domaine de la signalisation cellulaire présentant des résultats originaux, via des approches expérimentales variées, sur des thèmes allant des interactions hôte-pathogène au métabolisme, en passant par la compétition cellulaire et le microbiote. Après un travail préparatoire réalisé avec l’équipe pédagogique, les étudiants, organisés en binômes/trinômes, ont ensuite rédigé, guidés par des chercheurs, une Nouvelle soulignant les résultats majeurs et l’originalité de l’article étudié. Ils ont beaucoup apprécié cette initiation à l’écriture d’articles scientifiques et, comme vous pourrez le lire, se sont investis dans ce travail avec enthousiasme

Therapeutic drug monitoring of corticosteroids/β2-agonists in the hair of patients with asthma: an open-label feasibility study

International audienceBackground: Although adherence to inhaled medication is critically important for treatment efficiency, around half of patients taking these drugs are non-adherent or make critical errors when using their delivery device. Segmental hair analysis might be a valuable tool for therapeutic monitoring because hair concentrations reflect exposure from month to month. The objective of the present proof-of-concept study was to establish the feasibility of segmental hair analysis of inhaled budesonide and formoterol in asthma patients.Methods: We conducted a prospective, open-label, interventional study of adult patients being treated with budesonide/formoterol for controlled, moderate-to-severe asthma (CorticHair, NCT03691961). Asthma control, lung function, and medication adherence were recorded. Hair samples were taken 4 months after enrolment and cut into four 1 cm segments.Results: Samples were available from 21 patients (20 women; median age: 53; median budesonide dose: 600 μg/d). Budesonide and formoterol were detected in samples from 18 to 13 patients, respectively. The median hair concentrations were 6.25 pg/mg for budesonide and 0.9 pg/mg for formoterol. The intrapatient coefficient of variation between hair segments was 21% for budesonide and 40% for formoterol. Pearson’s coefficients for the correlations between the hair concentration and the self-reported drug dose and the prescribed drug dose were respectively 0.42 ( p = 0.08) and 0.29 ( p = 0.25) for budesonide and 0.24 ( p = 0.44) and 0.17 ( p = 0.57) for formoterol.Conclusion: Segmental hair analysis of inhaled medications was feasible, with low intrapatient variability. This innovative, non-invasive means of assessing monthly drug exposure might help physicians to personalize drug regimens for patients with difficult-to-treat asthma

Impact of temporal beach grain size variability on aeolian sediment transport and topographic evolution in a microtidal environment

While the impact of the spatio/temporal variability of grain size on morphological beach state is reasonably well understood, relatively little is known on its impact at a scale of days/months on aeolian sediment transport. This study focuses on five short intensive wind events during which aeolian sediment transport measurements, beach surface sampling and elevation change surveys were carried out for 1 to 3 days, over a 16-month period on a microtidal beach dominated by offshore winds. Monthly observations show a high temporal variability in beach grain size in relation to the decoupling between hydrodynamic and aeolian processes, from medium sand after a marine storm and inundation of the beach, to very coarse sand after several weeks of storm-force winds. During each wind event, topographic change on the beach ranged from zero, to 0.55 m. The time scale of coarsening depended on the initial beach grain size and could be very fast when the beach was composed of medium sand (e.g. 388 μm sand changed to coarse sand of 547 μm in 40 h). In contrast, it took one month to transition from a coarse median beach grain size of 883 μm to a very coarse one of 1323 μm. This variability in grain size results in dramatically different rates of sediment flux. For example, during average wind speeds of 10 to 14 m/s the sediment flux when the beach was composed of medium sized sand ranged between 21 and 154 kg/m/h compared to 0.4 to 50 kg/m/h when the beach was composed of coarse and very coarse sized grains. Overall, this study highlights the importance of beach grain size variability on aeolian sediment transport and shows that for similar incident wind and climatic conditions aeolian sediment transport rates vary dramatically. The study demonstrates the importance of taking care when using a constant median grain size in the calculation of long-term aeolian sand transport on beaches with heterogeneous spatial and temporal beach grain size variability

Démarche de prévention dans le secteur médico-social : MESOCAP

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