Sustainability and oxidase biocatalysis – An overview

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Panchromatic properties of galaxies in wide-field optical spectroscopic and photometric surveys

The past 15 years have seen an explosion in the number of redshifts recovered via wide area spectroscopic surveys. At the current time there are approximately 2million spectroscopic galaxy redshifts known (and rising) which represents an extraordinary growth since the pioneering work of Marc Davis and John Huchra. Similarly there has been a parallel explosion in wavelength coverage with imaging surveys progressing from single band, to multi-band, to truly multiwavelength or pan-chromatic involving the coordination of multiple facilities. With these empirically motivated studies has come a wealth of new discoveries impacting almost all areas of astrophysics. Today individual surveys, as best demonstrated by the Sloan Digital Sky Survey, now rank shoulder-to-shoulder alongside major facilities. In the coming years this trend is set to continue as we being the process of designing and conducting the next generation of spectroscopic surveys supported by multi-facility wavelength coverage.Comment: Invited review article to be published in Proceedings of IAU Symposium 284 on "The Spectral Energy Distribution of Galaxies", (Eds: R.J.Tuffs & C.C.Popescu

Biotechnological Potential of Oxidative Enzymes from Actinobacteria

Oxidative enzymes are often considered for use in industrial processes because of the variety of reactions they are able to catalyse. In the past, most of these oxidative enzymes were obtained from fungi. However, in recent years, it has become evident that these enzymes are also produced by bacteria, including actinobacterial strains, which can therefore be considered as an underexploited resource of oxidative enzymes with potential for application in various industries. This chapter will focus on selected oxidative enzymes found in actinobacteria, their potential for application in industrial processes and how we can access and improve these enzymes to suit the required bioprocess conditions

Long Duration Life Test of Propylene Glycol Water Based Thermal Fluid Within Thermal Control Loop

Evaluations of thermal properties and resistance to microbial growth concluded that 50% Propylene Glycol (PG)-based fluid and 50% de-ionized water mixture was desirable for use as a fluid within a vehicle s thermal control loop. However, previous testing with a commercial mixture of PG and water containing phosphate corrosion inhibitors resulted in corrosion of aluminum within the test system and instability of the test fluid. This paper describes a follow-on long duration testing and analysis of 50% Propylene Glycol (PG)-based fluid and 50% de-ionized water mixture with inorganic corrosion inhibitors used in place of phosphates. The test evaluates the long-term fluid stability and resistance to microbial and chemical change

Comparison of charge modulations in La1.875_{1.875}Ba0.125_{0.125}CuO4_4 and YBa2_2Cu3_3O6.6_{6.6}

A charge modulation has recently been reported in (Y,Nd)Ba$_2$Cu$_3$O$_{6+x}$ [Ghiringhelli {\em et al.} Science 337, 821 (2013)]. Here we report Cu $L_3$ edge soft x-ray scattering studies comparing the lattice modulation associated with the charge modulation in YBa$_2$Cu$_3$O$_{6.6}$ with that associated with the well known charge and spin stripe order in La$_{1.875}$Ba$_{0.125}$CuO$_4$. We find that the correlation length in the CuO$_2$ plane is isotropic in both cases, and is $259 \pm 9$ \AA for La$_{1.875}$Ba$_{0.125}$CuO$_4$ and $55 \pm 15$ \AA for YBa$_2$Cu$_3$O$_{6.6}$. Assuming weak inter-planar correlations of the charge ordering in both compounds, we conclude that the order parameters of the lattice modulations in La$_{1.875}$Ba$_{0.125}$CuO$_4$ and YBa$_2$Cu$_3$O$_{6.6}$ are of the same order of magnitude.Comment: 3 pages, 2 figure

Parametrisations of the D -> K l nu form factor and the determination of \hat{g}

The vector form factor f_+(t) of the semileptonic decay D -> K l nu, measured recently with a high accuracy, can be used to determine the strong coupling constant g_{D_s^* D K}. The latter is related to the normalised coupling \hat{g} releveant in heavy-meson chiral perturbation theory. This determination relies on the estimation of the residue of the form factor at the D_s^* pole and thus on an extrapolation of the form factor in the unphysical region (m_D-m_K)^2<t<(m_D+m_K)^2. We test this extrapolation for several parametrisations of the form factors by determining the value of \hat{g}, whose value can be compared to other (experimental and theoretical) estimates. Several unsophisticated parametrisations, differing by the amount of physical information that they embed, are shown to pass this test. An apparently more elaborated parametrisation of form factors, the so-called z-expansion, is at variance with the other models, and we point out some significant shortcomings of this parametrisation for the problem under consideration.Comment: 16 pages, 2 figures. A few references added. Accepted for publication in JoP

Cardiovascular risk profile and frailty in a population-based study of older British men.

BACKGROUND: Frailty in older age is known to be associated with cardiovascular disease (CVD) risk. However, the extent to which frailty is associated with the CVD risk profile has been little studied. Our aim was to examine the associations of a range of cardiovascular risk factors with frailty and to assess whether these are independent of established CVD. METHODS: Cross-sectional study of a socially representative sample of 1622 surviving men aged 71-92 examined in 2010-2012 across 24 British towns, from a prospective study initiated in 1978-1980. Frailty was defined using the Fried phenotype, including weight loss, grip strength, exhaustion, slowness and low physical activity. RESULTS: Among 1622 men, 303 (19%) were frail and 876 (54%) were pre-frail. Compared with non-frail, those with frailty had a higher odds of obesity (OR 2.03, 95% CI 1.38 to 2.99), high waist circumference (OR 2.30, 95% CI 1.67 to 3.17), low high-density lipoprotein-cholesterol (HDL-C) (OR 2.28, 95% CI 1.47 to 3.54) and hypertension (OR 1.79, 95% CI 1.27 to 2.54). Prevalence of these factors was also higher in those with frailty (prevalence in frail vs non-frail groups was 46% vs 31% for high waist circumference, 20% vs 11% for low HDL and 78% vs 65% for hypertension). Frail individuals had a worse cardiovascular risk profile with an increased risk of high heart rate, poor lung function (forced expiratory volume in 1 s (FEV1)), raised white cell count (WCC), poor renal function (low estimated glomerular filtration rate), low alanine transaminase and low serum sodium. Some risk factors (HDL-C, hypertension, WCC, FEV1, renal function and albumin) were also associated with being pre-frail. These associations remained when men with prevalent CVD were excluded. CONCLUSIONS: Frailty was associated with increased risk of a range of cardiovascular factors (including obesity, HDL-C, hypertension, heart rate, lung function, renal function) in older people; these associations were independent of established CVD

Inelastic x-ray scattering investigations of lattice dynamics in SmFeAsO1−x_{1-x}Fy_y superconductors

We report measurements of the phonon density of states as measured with inelastic x-ray scattering in SmFeAsO$_{1-x}$F$_y$ powders. An unexpected strong renormalization of phonon branches around 23 meV is observed as fluorine is substituted for oxygen. Phonon dispersion measurements on SmFeAsO$_{1-x}$F$_y$ single crystals allow us to identify the 21 meV A$_{1g}$ in-phase (Sm,As) and the 26 meV B$_{1g}$ (Fe,O) modes to be responsible for this renormalization, and may reveal unusual electron-phonon coupling through the spin channel in iron-based superconductors.Comment: 4 pages, 3 figures, submitted for SNS2010 conference proceeding

Hydrogel microparticles for biosensing

Due to their hydrophilic, biocompatible, and highly tunable nature, hydrogel materials have attracted strong interest in the recent years for numerous biotechnological applications. In particular, their solution-like environment and non-fouling nature in complex biological samples render hydrogels as ideal substrates for biosensing applications. Hydrogel coatings, and later, gel dot surface microarrays, were successfully used in sensitive nucleic acid assays and immunoassays. More recently, new microfabrication techniques for synthesizing encoded particles from hydrogel materials have enabled the development of hydrogel-based suspension arrays. Lithography processes and droplet-based microfluidic techniques enable generation of libraries of particles with unique spectral or graphical codes, for multiplexed sensing in biological samples. In this review, we discuss the key questions arising when designing hydrogel particles dedicated to biosensing. How can the hydrogel material be engineered in order to tune its properties and immobilize bioprobes inside? What are the strategies to fabricate and encode gel particles, and how can particles be processed and decoded after the assay? Finally, we review the bioassays reported so far in the literature that have used hydrogel particle arrays and give an outlook of further developments of the field. Keywords: Hydrogel; Biosensor; Microparticle; Multiplex assayNovartis Institutes of Biomedical Research (Presidential Fellowship)Novartis Institutes of Biomedical Research (Education Office)National Cancer Institute (U.S.) (Grant 5R21CA177393-02)National Science Foundation (U.S.) (Grant CMMI-1120724)Institute for Collaborative Biotechnologies (Grant W911NF-09-0001)United States. Army Research Offic