Array phasing device Patent

Apparatus for generating microwave signals at progressively related phase angles for driving antenna arra

Cryo-Electron Tomography Elucidates the Molecular Architecture of Treponema pallidum, the Syphilis Spirochete

Cryo-electron tomography (CET) was used to examine the native cellular organization of Treponema pallidum, the syphilis spirochete. T. pallidum cells appeared to form flat waves, did not contain an outer coat and, except for bulges over the basal bodies and widening in the vicinity of flagellar filaments, displayed a uniform periplasmic space. Although the outer membrane (OM) generally was smooth in contour, OM extrusions and blebs frequently were observed, highlighting the structure’s fluidity and lack of attachment to underlying periplasmic constituents. Cytoplasmic filaments converged from their attachment points opposite the basal bodies to form arrays that ran roughly parallel to the flagellar filaments along the inner surface of the cytoplasmic membrane (CM). Motile treponemes stably attached to rabbit epithelial cells predominantly via their tips. CET revealed that T. pallidum cell ends have a complex morphology and assume at least four distinct morphotypes. Images of dividing treponemes and organisms shedding cell envelope-derived blebs provided evidence for the spirochete’s complex membrane biology. In the regions without flagellar filaments, peptidoglycan (PG) was visualized as a thin layer that divided the periplasmic space into zones of higher and lower electron densities adjacent to the CM and OM, respectively. Flagellar filaments were observed overlying the PG layer, while image modeling placed the PG-basal body contact site in the vicinity of the stator–P-collar junction. Bioinformatics and homology modeling indicated that the MotB proteins of T. pallidum, Treponema denticola, and Borrelia burgdorferi have membrane topologies and PG binding sites highly similar to those of their well-characterized Escherichia coli and Helicobacter pylori orthologs. Collectively, our results help to clarify fundamental differences in cell envelope ultrastructure between spirochetes and gram-negative bacteria. They also confirm that PG stabilizes the flagellar motor and enable us to propose that in most spirochetes motility results from rotation of the flagellar filaments against the PG

Mapping the Shores of the Brown Dwarf Desert III: Young Moving Groups

We present the results of an aperture masking interferometry survey for substellar companions around 67 members of the young (~8-200Myr) nearby (~5-86pc) AB Doradus, Beta Pictoris, Hercules-Lyra, TW Hya, and Tucana-Horologium stellar associations. Observations were made at near infrared wavelengths between 1.2-3.8 microns using the adaptive optics facilities of the Keck II, VLT UT4, and Palomar Hale Telescopes. Typical contrast ratios of ~100-200 were achieved at angular separations between ~40-320mas, with our survey being 100% complete for companions with masses below 0.25\msolar across this range. We report the discovery of a $0.52 \pm 0.09$\msolar companion to HIP14807, as well as the detections and orbits of previously known stellar companions to HD16760, HD113449, and HD160934. We show that the companion to HD16760 is in a face-on orbit, resulting in an upward revision of its mass from $M_2 \sin i \sim 14$\mjupiter to $M_2 = 0.28 \pm 0.04$\msolar. No substellar companions were detected around any of our sample members, despite our ability to detect companions with masses below 80\mjupiter for 50 of our targets: of these, our sensitivity extended down to 40\mjupiter around 30 targets, with a subset of 22 subject to the still more stringent limit of 20\mjupiter. A statistical analysis of our non-detection of substellar companions allows us to place constraints on their frequency around ~0.2-1.5\msolar stars. In particular, considering companion mass distributions that have been proposed in the literature, we obtain an upper limit estimate of ~9-11% for the frequency of 20-80\mjupiter companions between 3-30AU at 95% confidence, assuming that their semimajor axes are distributed according to $d\mathcal{N}/da \propto a^{-1}$ in this range.Comment: Accepted by Ap

Macrophage mediated recognition and clearance of Borrelia burgdorferi elicits MyD88-dependent and -independent phagosomal signals that contribute to phagocytosis and inflammation.

BACKGROUND: Macrophages play prominent roles in bacteria recognition and clearance, including Borrelia burgdorferi (Bb), the Lyme disease spirochete. To elucidate mechanisms by which MyD88/TLR signaling enhances clearance of Bb by macrophages, we studied wildtype (WT) and MyD88 RESULTS: MyD88 CONCLUSION: Our findings show that MyD88 signaling enhances, but is not required, for bacterial uptake or phagosomal maturation and provide mechanistic insights into how MyD88-mediated phagosomal signaling enhances Bb uptake and clearance

The age-redshift relation for Luminous Red Galaxies in the Sloan Digital Sky Survey

We present a detailed analysis of 17,852 quiescent, Luminous Red Galaxies (LRGs) selected from Sloan Digital Sky Survey (SDSS) Data Release Seven (DR7) spanning a redshift range of 0.0 < z < 0.4. These galaxies are co-added into four equal bins of velocity dispersion and luminosity to produce high signal-to-noise spectra (>100A^{-1}), thus facilitating accurate measurements of the standard Lick absorption-line indices. In particular, we have carefully corrected and calibrated these indices onto the commonly used Lick/IDS system, thus allowing us to compare these data with other measurements in the literature, and derive realistic ages, metallicities ([Z/H]) and alpha-element abundance ratios ([alpha/Fe]) for these galaxies using Simple Stellar Population (SSP) models. We use these data to study the relationship of these galaxy parameters with redshift, and find little evidence for evolution in metallicity or alpha-elements (especially for our intermediate mass samples). This demonstrates that our subsamples are consistent with pure passive evolving (i.e. no chemical evolution) and represent a homogeneous population over this redshift range. We also present the age-redshift relation for these LRGs and clearly see a decrease in their age with redshift (5 Gyrs over the redshift range studied here) which is fully consistent with the cosmological lookback times in a concordance Lambda CDM universe. We also see that our most massive sample of LRGs is the youngest compared to the lower mass galaxies. We provide these data now to help future cosmological and galaxy evolution studies of LRGs, and provide in the appendices of this paper the required methodology and information to calibrate SDSS spectra onto the Lick/IDS system.Comment: 26 pages, with several appendices containing data. Accepted for publication in MNRA

Doctor of Education Newsletter 2019

WSU Doctor of Education Inaugural Cohort 2019 Winona State University has an astounding reputation for educational practitioner preparation programs in the College of Education. As the summer of 2019 commences, a new journey begins for the inaugural cohort of doctoral students who embark on their quest to obtain Doctor of Education degrees.https://openriver.winona.edu/educationeddnewsletters/1000/thumbnail.jp

Doctoral Student Perspectives on Motivation and Persistence: Eye-Opening Insights Into the Ideas and Thoughts That Today\u27s Doctoral Students Have About Finishing the Doctoral Degree

It all comes down to this: we have an amazing team of faculty working with us who are present, supportive, intelligent, and motivated to help us succeed. They designed this program with those objectives in mind. We are in good hands, and any questions we have will be answered, so long as we ask them. Having the support system of our faculty, along with the tools we need to be successful, are major parts of the battle, already won. The rest is up to us. - A. Brooke Boultonhttps://openriver.winona.edu/educationeddbooks/1000/thumbnail.jp

Development of an in vitro three dimensional loading-measurement system for long bone fixation under multiple loading conditions: a technical description

The purpose of this investigation was to design and verify the capabilities of an in vitro loading-measurement system that mimics in vivo unconstrained three dimensional (3D) relative motion between long bone ends, applies uniform load components over the entire length of a test specimen, and measures 3D relative motion between test segment ends to directly determine test segment construct stiffness free of errors due to potting-fixture-test machine finite stiffness

Evidence for the role of EPHX2 gene variants in anorexia nervosa.

Anorexia nervosa (AN) and related eating disorders are complex, multifactorial neuropsychiatric conditions with likely rare and common genetic and environmental determinants. To identify genetic variants associated with AN, we pursued a series of sequencing and genotyping studies focusing on the coding regions and upstream sequence of 152 candidate genes in a total of 1205 AN cases and 1948 controls. We identified individual variant associations in the Estrogen Receptor-ß (ESR2) gene, as well as a set of rare and common variants in the Epoxide Hydrolase 2 (EPHX2) gene, in an initial sequencing study of 261 early-onset severe AN cases and 73 controls (P=0.0004). The association of EPHX2 variants was further delineated in: (1) a pooling-based replication study involving an additional 500 AN patients and 500 controls (replication set P=0.00000016); (2) single-locus studies in a cohort of 386 previously genotyped broadly defined AN cases and 295 female population controls from the Bogalusa Heart Study (BHS) and a cohort of 58 individuals with self-reported eating disturbances and 851 controls (combined smallest single locus P&lt;0.01). As EPHX2 is known to influence cholesterol metabolism, and AN is often associated with elevated cholesterol levels, we also investigated the association of EPHX2 variants and longitudinal body mass index (BMI) and cholesterol in BHS female and male subjects (N=229) and found evidence for a modifying effect of a subset of variants on the relationship between cholesterol and BMI (P&lt;0.01). These findings suggest a novel association of gene variants within EPHX2 to susceptibility to AN and provide a foundation for future study of this important yet poorly understood condition