Research of the Influence on the Modification of Beryllium in Al–Si Alloy

Modification of molten metal is performed for the eutectic and subeutectic silumins prior to its casting by using strontium, which has a time-limited effect up to 1–1.5 hours. Beryllium is used in order to prolong the modifying effect. The aim of the experiment is to examine both the distribution and the effect of beryllium in the structure on the temporary extension of the modification in the Al–Si alloys in combination with strontium modifier.Перед отливкой расплавленного металла выполняется модифицирование эвтектических и субэвтектических силуминов с помощью стронция, который имеет ограниченный 1–1,5 часами временный эффект воздействия. Бериллий используется для продления модифицирующего эффекта. Целью эксперимента является изучение распределения и влияния бериллия в структуре на временное продление модифицирующего воздействия в сплавах Al–Si в сочетании со стронциевым модификатором.Перед відливанням розтопленого металу виконується модифікування евтектичних і субевтектичних силумінів за допомогою Стронцію, який має обмежений 1–1,5 годинами тимчасовий ефект дії. Берилій використовується для продовження модифікувального ефекту. Метою експерименту є вивчення розподілу та впливу берилію в структурі на тимчасове подовження модифікувальної дії в стопах Al–Si у поєднанні зі стронційовим модифікатором

clostridium perfringens occurrence and ribotypes in healthy broilers reared in different european countries

Abstract The main aim of this study was to investigate the occurrence and ribotypes of Clostridium perfringens in broiler flocks reared in 2 European countries that apply European Union Regulation 1831/2003. A total of 1,532 cecum contents were collected between June 2005 and November 2006 from birds belonging to 51 intensively reared flocks produced in the Czech Republic and 41 intensive production, organic, and free-range flocks reared in Italy. Clostridium perfringens was detected in 64.7 and 82.9% of the Czech Republic and Italian flocks, respectively, at mean loads ranging between 3.65 and 4.77 log10 cfu per gram of cecum content. More than 1 ribotype was identified among isolates belonging to the same flock in 57.1 and 76.5% of the Czech Republic and Italian flocks, respectively. Moreover, common ribotypes were identified between strains belonging to 2 up to 8 different flocks. In particular, 4 ribotypes were shared between strains isolated in the 2 European countries. The results of this study report on C. perfringens occurrence and mean populations in broilers reared on diets devoid of antibiotic growth promoters. Moreover, these findings show for the first time the presence of common ribotyping profiles among isolates collected from birds reared more than 1,000 km apart

Changes in species composition and diversity of a montane beetle community over the last millennium in the High Tatras, Slovakia : Implications for forest conservation and management

Montane biomes are niche environments high in biodiversity with a variety of habitats. Often isolated, these non-continuous remnant ecosystems inhabit narrow ecological zones putting them under threat from changing climatic conditions and anthropogenic pressure. Twelve sediment cores were retrieved from a peat bog in Tatra National Park, Slovakia, and correlated to each other by wiggle-matching geochemical signals derived from micro-XRF scanning, to make a reconstruction of past conditions. A fossil beetle (Coleoptera) record, covering the last 1000 years at 50- to 100-year resolution, gives a new insight into changing flora and fauna in this region. Our findings reveal a diverse beetle community with varied ecological groups inhabiting a range of forest, meadow and synanthropic habitats. Changes in the beetle community were related to changes in the landscape, driven by anthropogenic activities. The first clear evidence for human activity in the area occurs c. 1250 CE and coincides with the arrival of beetle species living on the dung of domesticated animals (e.g. Aphodius spp.). From 1500 CE, human (re)settlement, and activities such as pasturing and charcoal burning, appear to have had a pronounced effect on the beetle community. Local beetle diversity declined steadily towards the present day, likely due to an infilling of the forest hollow leading to a decrease in moisture level. We conclude that beetle communities are directly affected by anthropogenic intensity and land-use change. When aiming to preserve or restore natural forest conditions, recording their past changes in diversity can help guide conservation and restoration. In doing so, it is important to look back beyond the time of significant human impact, and for this, information contained in paleoecological records is irreplaceable.Peer reviewe

Tailored antisense oligonucleotides designed to correct aberrant splicing reveal actionable groups of mutations for rare genetic disorders

Effective translation of rare disease diagnosis knowledge into therapeutic applications is achievable within a reasonable timeframe; where mutations are amenable to current antisense oligonucleotide technology. In our study, we identified five distinct types of abnormal splice-causing mutations in patients with rare genetic disorders and developed a tailored antisense oligonucleotide for each mutation type using phosphorodiamidate morpholino oligomers with or without octa-guanidine dendrimers and 2′-O-methoxyethyl phosphorothioate. We observed variations in treatment effects and efficiencies, influenced by both the chosen chemistry and the specific nature of the aberrant splicing patterns targeted for correction. Our study demonstrated the successful correction of all five different types of aberrant splicing. Our findings reveal that effective correction of aberrant splicing can depend on altering the chemical composition of oligonucleotides and suggest a fast, efficient, and feasible approach for developing personalized therapeutic interventions for genetic disorders within short time frames

Acetylcholinesterase sensors based on gold electrodes modified with dendrimer and polyaniline: A comparative research

Potentiometric and amperometric enzyme sensors based on modified gold electrodes have been developed and compared in pesticide determination. PAMAM dendrimer (generation G4) stabilized with 1-hexadecanethiol was used for the immobilization of acetylcholinesterase from electric eel and choline oxidase from Alcaligenes species in the assembly of amperometric sensor. Polyaniline-doped with camphorsulfonic acid was used to obtain potentiometric response. Trichlorfon, carbofuran and eserine suppress the biosensor response due to their inhibitory effect. The detection limits of 0.003 and 200 nmol l-1 (trichlorfon), 0.04 and 6 nmol l-1 (carbofuran) and 0.1 and 700 nmol l-1 were obtained for amperometric and potentiometric sensors, respectively. The difference in the biosensor behavior and the high sensitivity of the dendrimer modified sensor to the inhibitors is due to the specific organization of protein layer at charged surface of the modifier macromolecules. © 2004 Elsevier B.V. All rights reserved

Dysregulation of epicardial adipose tissue in cachexia due to heart failure. the role of natriuretic peptides and cardiolipin

Background: Cachexia worsens long-term prognosis of patients with heart failure (HF). Effective treatment of cachexia is missing. We seek to characterize mechanisms of cachexia in adipose tissue, which could serve as novel targets for the treatment. Methods: The study was conducted in advanced HF patients (n = 52; 83% male patients) undergoing heart transplantation. Patients with ≥7.5% non-intentional body weight (BW) loss during the last 6 months were rated cachectic. Clinical characteristics and circulating markers were compared between cachectic (n = 17) and the remaining, BW-stable patients. In epicardial adipose tissue (EAT), expression of selected genes was evaluated, and a combined metabolomic/lipidomic analysis was performed to assess (i) the role of adipose tissue metabolism in the development of cachexia and (ii) potential impact of cachexia-associated changes on EAT-myocardium environment. Results: Cachectic vs. BW-stable patients had higher plasma levels of natriuretic peptide B (BNP; 2007 ± 1229 vs. 1411 ± 1272 pg/mL; P = 0.010) and lower EAT thickness (2.1 ± 0.8 vs. 2.9 ± 1.4 mm; P = 0.010), and they were treated with ~2.5-fold lower dose of both β-blockers and angiotensin-converting enzyme inhibitors or angiotensin receptor blockers (ACE/ARB-inhibitors). The overall pattern of EAT gene expression suggested simultaneous activation of lipolysis and lipogenesis in cachexia. Lower ratio between expression levels of natriuretic peptide receptors C and A was observed in cachectic vs. BW-stable patients (0.47 vs. 1.30), supporting activation of EAT lipolysis by natriuretic peptides. Fundamental differences in metabolome/lipidome between BW-stable and cachectic patients were found. Mitochondrial phospholipid cardiolipin (CL), specifically the least abundant CL 70:6 species (containing C16:1, C18:1, and C18:2 acyls), was the most discriminating analyte (partial least squares discriminant analysis; variable importance in projection score = 4). Its EAT levels were higher in cachectic as compared with BW-stable patients and correlated with the degree of BW loss during the last 6 months (r = −0.94; P = 0.036). Conclusions: Our results suggest that (i) BNP signalling contributes to changes in EAT metabolism in cardiac cachexia and (ii) maintenance of stable BW and ‘healthy’ EAT-myocardium microenvironment depends on the ability to tolerate higher doses of both ACE/ARB inhibitors and β-adrenergic blockers. In line with preclinical studies, we show for the first time in humans the association of cachexia with increased adipose tissue levels of CL. Specifically, CL 70:6 could precipitate wasting of adipose tissue, and thus, it could represent a therapeutic target to ameliorate cachexia

Biopolymer-based structuring of liquid oil into soft solids and oleogels using water-continuous emulsions as templates

Physical trapping of a hydrophobic liquid oil in a matrix of water-soluble biopolymers was achieved using a facile two-step process by first formulating a surfactant-free oil-in-water emulsion stabilized by biopolymers (a protein and a polysaccharide) followed by complete removal of the water phase (by either high- or low-temperature drying of the emulsion) resulting in structured solid systems containing a high concentration of liquid oil (above 97 wt %). The microstructure of these systems was revealed by confocal and cryo-scanning electron microscopy, and the effect of biopolymer concentrations on the consistency of emulsions as well as the dried product was evaluated using a combination of small-amplitude oscillatory shear rheometry and large deformation fracture studies. The oleogel prepared by shearing the dried product showed a high gel strength as well as a certain degree of thixotropic recovery even at high temperatures. Moreover, the reversibility of the process was demonstrated by shearing the dried product in the presence of water to obtain reconstituted emulsions with rheological properties comparable to those of the fresh emulsion

Oral treatment of 4-methylumbelliferone reduced perineuronal nets and improved recognition memory in mice

Hyaluronan (HA) is a core constituent of perineuronal nets (PNNs) that surround subpopulations of neurones. The PNNs control synaptic stabilization in both the developing and adult central nervous system, and disruption of PNNs has shown to reactivate neuroplasticity. We investigated the possibility of memory prolongation by attenuating PNN formation using 4-methylumbelliferone (4-MU), an inhibitor of HA synthesis. Adult C57BL/6 mice were fed with chow containing 5% (w/w) 4-MU for 6 months, at a dose ~6.7 mg/g/day. The oral administration of 4-MU reduced the glycosaminoglycan level in the brain to 72% and the spinal cord to 50% when compared to the controls. Spontaneous object recognition test (SOR) performed at 2, 3, 6 and 7 months showed a significant increase in SOR score in the 6-months treatment group 24 h after object presentation. The effect however did not persist in the washout group (1-month post treatment). Immunohistochemistry confirmed a reduction of PNNs, with shorter and less arborization of aggrecan staining around dendrites in hippocampus after 6 months of 4-MU treatment. Histopathological examination revealed mild atrophy in articular cartilage but it did not affect the motor performance as demonstrated in rotarod test. In conclusion, systemic oral administration of 4-MU for 6 months reduced PNN formation around neurons and enhanced memory retention in mice. However, the memory enhancement was not sustained despite the reduction of PNNs, possibly due to the lack of memory enhancement training during the washout period. Our results suggest that 4-MU treatment might offer a strategy for PNN modulation in memory enhancement

The proposal of a GSSP for the Berriasian Stage (Cretaceous System): Part 1.

Here in the first part of this publication we discuss the possibilities for the selection of a GSSP for the Berriasian Stage of the Cretaceous System, based on the established methods for correlation in the Tithonian/Berriasian interval. This will be followed, in the second part, by an account of the stratigraphic evidence that justifies the locality of Tré Maroua (Hautes-Alpes, SE France) as the proposed GSSP. Here we discuss the possibilities for correlation in the historical J/K boundary interval, and the evolution of thinking on the positioning of the boundary over recent generations, and in relation to research in the last ten years. The Tithonian/Berriasian boundary level is accepted as occurring within magnetosubzone M19n.2n. The detailed distribution of calpionellids has been recorded at numerous sites, tied to magnetostratigraphy, and the base of the calpionellid Alpina Zone is taken to define the base of the Berriasian Stage. This is at a level just below the distinctive reversed magnetic subzone M19n.1r (the so-called Brodno reversal). We discuss a wide range of magnetostratigraphic and biostratigraphic data from key localities globally, in the type Berriasian areas of France and wider regions (Le Chouet, Saint Bertrand, Puerto Escaño, Rio Argos, Bosso, Brodno, Kurovice, Theodosia etc.). The characteristic datums that typify the J/K boundary interval in Tethys and its extensions are detailed, and the correlative viability of various fossil groups is discussed. The boundary level is correlated to well-known J/K sections globally, and a series of secondary markers and proxies are indicated which assist wider correlation. Particularly significant are the primary basal Berriasian marker, the base of the Alpina Subzone (marked by dominance of small Calpionella alpina, Crassicollaria parvula and Tintinopsella carpathica) and secondary markers bracketing the base of the Calpionella Zone, notably the FOs of the calcareous nannofossil species Nannoconus wintereri (just below the boundary) and the FO of Nannoconus steinmannii minor (just above). Notable proxies for the boundary are: 1) the base of the Arctoteuthis tehamaensis Zone in boreal and subboreal regions, 2) the dated base of the Alpina Subzone at 140.22 ± 0.14 Ma, which also gives a precise age estimate for the system boundary; and 3) the base of radiolarian “unitary zone” 14, which is situated just above the base of the Alpina Subzone

Detection and Identification of Old World Leishmania by High Resolution Melt Analysis

Protozoal parasites of the genus Leishmania are transmitted by sand fly bites to humans and animals. Three major forms of disease are caused by these parasites: cutaneous leishmaniasis, responsible for disfiguring skin wounds; mucocutaneous leishmaniasis, causing non-healing ulceration around the mouth and nose; and the potentially fatal visceral leishmaniasis, involving internal organs such as the spleen and liver. More than 2 million new human infections are caused annually by leishmaniasis globally, it is endemic in more than 88 countries and prevalent also as an imported disease in non-endemic regions due to travel and tourism. Most species of Leishmania that infect humans are zoonotic and transmitted from animal reservoir hosts. As various leishmanial parasites cause disease with similar symptoms, but require different therapeutic regimens and have dissimilar prognoses, reliable, sensitive and rapid diagnostic assays are needed. This study focuses on the five main species that cause leishmaniasis in the Old World. It presents a new assay for rapid detection, species identification and quantification of leishmanial parasites in clinical samples, reservoir hosts and sand flies. This technique could be especially valuable in regions where several leishmanial species exist, in non-endemic regions where infected patients require a rapid diagnosis, and for epidemiological host and vector studies leading to prevention programs