Photography-based taxonomy is inadequate, unnecessary, and potentially harmful for biological sciences

The question whether taxonomic descriptions naming new animal species without type specimen(s) deposited in collections should be accepted for publication by scientific journals and allowed by the Code has already been discussed in Zootaxa (Dubois & Nemésio 2007; Donegan 2008, 2009; Nemésio 2009a–b; Dubois 2009; Gentile & Snell 2009; Minelli 2009; Cianferoni & Bartolozzi 2016; Amorim et al. 2016). This question was again raised in a letter supported by 35 signatories published in the journal Nature (Pape et al. 2016) on 15 September 2016. On 25 September 2016, the following rebuttal (strictly limited to 300 words as per the editorial rules of Nature) was submitted to Nature, which on 18 October 2016 refused to publish it. As we think this problem is a very important one for zoological taxonomy, this text is published here exactly as submitted to Nature, followed by the list of the 493 taxonomists and collection-based researchers who signed it in the short time span from 20 September to 6 October 2016

Effects of acid mine drainage and acidity on the activity of choroterpes picteti (Ephemeroptera : Leptophlebiidae)

Survival and behavior of the mayfly Choroterpes picteti (Leptophlebiidae) exposed to acid mine drainage (AMD: pH 3.3-6.4) and a reservoir polluted with arsenic (pH 6.8) from Sao Domingos mine (Portugal) were studied in laboratory and in situ bioassays (48 h) with the Multispecies Freshwater Biomonitor, and compared with water from a reference river and acidified reference water (acid only). Metal body-burdens showed a negative pH dependency for Mn and As, a positive one for Pb, and for Zn, Cu, Co, and Cd a decrease at pH < 4.4. Generally, survival decreased with decreasing pH. The 48-h LC50 (pH) for AMD and for acid only were similar (pH 4.8-4.9); however, the LT,0 (h) at pH 3.3 revealed AMD to be less toxic than acid only. C. picteti show diurnal rhythm with increased locomotor activity in the night. The circadian rhythm was weakened by acid exposure, but less so by AMD exposure. Compared to reference river water, ventilation was stimulated at pH < 6.0 in acid only and in reservoir water. Locomotion was stimulated at pH 5 in acid only and reservoir, however, it was reduced in all other treatments, when compared to reference river water. Under acid-only exposure, both locomotion and ventilation were significantly higher compared to AMD exposure at the corresponding pH values. The laboratory results were field validated

Chironomidae larvae as bioindicators of an acid mine drainage in Portugal

The abandoned Sao Domingos mine in South Portugal discharges acid metal pollution in the drainage system. A dynamic pH- and metal-gradient (pH 3.3-6.8) modulated by rainfall at the confluence of a tributary with the acid mine drainage (AMD)-channel, and a small reservoir contaminated with arsenic were sampled for Chironomidae in three different seasons and compared with a control river, to test whether this insect family is sensitive to AMD-pollution and arsenic. The AMD sites contained 18-22 taxa, compared to 22 taxa in the reservoir and 25 taxa in the control site. The chironomid fauna in the AMD was characterised by a high proportion of Chironominae and predatory Tanypodinae, and a low proportion of Orthocladiinae, these subfamilies being useful and easy bioindicators for AMD. The presence of morphological deformities in Ablabesmyia monilis and Procladius sp. is a potential bioindication of AMD but due to low numbers of larvae, it was of limited use. Despite high arsenic levels in the water, the small reservoir contained many taxa forming a typical lentic community.CETERAIAV/82/ 00PRAXIS/C/MGS/10200/199