Scale-up of an intensified bioprocess for the expansion of bovine adipose-derived stem cells (bASCs) in stirred tank bioreactors

Cultivated meat is an emerging field, aiming to establish the production of animal tissue for human consumption in an in vitro environment, eliminating the need to raise and slaughter animals for their meat. To realise this, the expansion of primary cells in a bioreactor is needed to achieve the high cell numbers required. The aim of this study was to develop a scalable, microcarrier based, intensified bioprocess for the expansion of bovine adipose-derived stem cells as precursors of fat and muscle tissue. The intensified bioprocess development was carried out initially in spinner flasks of different sizes and then translated to fully controlled litre scale benchtop bioreactors. Bioprocess intensification was achieved by utilising the previously demonstrated bead-to-bead transfer phenomenon and through the combined addition of microcarrier and medium to double the existing surface area and working volume in the bioreactor. Choosing the optimal time point for the additions was critical in enhancing the cell expansion. A significant fold increase of 114.19 ± 1.07 was obtained at the litre scale in the intensified bioprocess compared to the baseline (**p < .005). The quality of the cells was evaluated pre- and post-expansion and the cells were found to maintain their phenotype and differentiation capacity

Chemin faisant. Mythes, cultes et société en Grèce ancienne. Mélanges en l'honneur de Pierre Brulé

Internationalement reconnu pour ses travaux sur la religion et la société grecques antiques, Pierre Brulé ne cesse de déconstruire le « miracle grec » en s'intéressant tout d'abord à ses marges, ses fractures, ses ombres. Les études rassemblées dans cet ouvrage s'inscrivent dans cette perspective en proposant de nouvelles approches, essentiellement dans trois domaines : l'histoire des femmes, l'histoire religieuse et l'histoire du corps. Ces trois domaines bien évidemment se recoupent, et l'analyse de la participation des femmes aux rituels et aux fêtes religieuses grecques, que ce soit à Athènes, à Olympie, à Delphes, ou bien encore dans les Lois de Platon, est complétée par l'étude d'associations, de magistrats, de l'usage du fouet… qui sont autant de manières de mettre en lumière comment les cités intégraient et contrôlaient leurs membres, quels que soient leur genre, leur identité, leur statut, n'hésitant pas à recourir à la violence s'il le fallait. De même, les réflexions proposées sur la médecine, les vêtements, les offrandes sacrificielles et le polythéisme, à partir de la prise en compte de mythes, de représentations, des noms des dieux, permettent de nouveau de modifier le regard porté sur le monde antique, en accordant bien souvent une place toute particulière au féminin. Ce refus des idées convenues, ce souci de donner la parole à ceux que les sources ont généralement laissé dans l'ombre, cette attention portée tout autant aux faits les plus concrets qu'à l'imaginaire, témoignent ainsi du renouvellement actuel de l'histoire de l'Antiquité, dont Pierre Brulé est, Chemin faisant, l'un des acteurs importants

Sub-MHz linewidth UV laser diode for metrological applications

International audienc

Sub-20 kHz low frequency noise near ultraviolet miniature external cavity laser diode

We present a compact InGaN fiber Bragg grating (FBG) semiconductor laser diode operating below 400 nm in the single-mode emission regime. This compact coherent laser source exhibits an intrinsic linewidth of 14 kHz in the near-UV range and a side-mode suppression ratio reaching up to 40 dB accompanied by a mW-level output power. Furthermore, the properties of the FBG, including its central wavelength, bandwidth, and reflectivity, can be readily customized to fulfill specific requirements. As a result, the small footprint design of this laser is compatible with integration into a standard butterfly package to ease the lab-to-market technology transfer. The combination of low frequency noise and fibered output signal positions these FBG laser systems as strong candidates for hybridization with integrated photonic platforms tailored for quantum information processing and metrology

Luminescence at 2.8 μm: Er3+-doped chalcogenide micro-waveguide

International audienc

Nature and origin of V-defects present in metalorganic vapor phase epitaxy-grown (InxAl1-x)N layers as a function of InN content, layer thickness and growth parameters

Our study of samples grown in different metalorganic chemical vapor deposition reactors and with different growth conditions reveals that V-pits are always present in (InxAl1-x)N films whatever the layer thickness and the InN content. V-pits are empty inverted pyramids terminating threading dislocations. InN-rich triangular regions are present around the threading dislocations terminated by pits with a hexagonal 6-fold symmetry distribution in {11 - 20} planes. The nature of the facets of the V-pits depends on the growth conditions: pits with either {11 - 2l}, I being between 1 and 3, or {1 - 101} facets have been observed. Moreover, the nature of the threading dislocations terminated by pits also depends on the growth conditions. Our observations suggest that with a high V/III ratio only edge a + c-type dislocations are terminated by pits whereas with a low V/III ratio both edge a-type and mixed a + c-type dislocations are terminated by pits

Development of an evanescent optical integrated sensor in the mid-infrared for detection of pollution in groundwater or seawater

International audienc