27 research outputs found

    Viscous and filamentous bulking in activated sludge: Rheological and hydrodynamic modelling based on experimental data

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    Although achieving good activated sludge settleability is a key requirement for meeting effluent quality criteria, wastewater treatment plants often face undesired floc structure changes. Filamentous bulking has widely been studied, however, viscous sludge formation much less investigated so far. Our main goal was to find relationship between sludge floc structure and related rheological properties, moreover, to estimate pressure loss in pipe networks through hydrodynamic modelling of the non-Newtonian flows in case of well settling (ideal-like), viscous and filamentous sludge. Severe viscous and filamentous kinds of bulking were generated separately in continuous-flow lab-scale systems initially seeded with the same reference (ideal-like) biomass and the entire evolution of viscous and filamentous bulking was monitored. The results suggested correlation between the rheological properties and the floc structure transformations, and showed the most appropriate fit for the Herschel-Bulkley model (vs. Power-law and Bingham). Validated computational fluid dynamics studies estimated the pipe pressure loss in a wide Reynolds number range for the initial well settling (reference) and the final viscous and filamentous sludge as well. A practical standard modelling protocol was developed for improving energy efficiency of sludge pumping in different floc structure scenarios

    Physicochemical and biological characterization of chitosan-microRNA nanocomplexes for gene delivery to MCF-7 breast cancer cells

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    Cancer gene therapy requires the design of non-viral vectors that carry genetic material and selectively deliver it with minimal toxicity. Non-viral vectors based on cationic natural polymers can form electrostatic complexes with negatively-charged polynucleotides such as microRNAs (miRNAs). Here we investigated the physicochemical/biophysical properties of chitosan–hsa-miRNA-145 (CS–miRNA) nanocomplexes and the biological responses of MCF-7 breast cancer cells cultured in vitro. Self-assembled CS–miRNA nanocomplexes were produced with a range of (+/−) charge ratios (from 0.6 to 8) using chitosans with various degrees of acetylation and molecular weight. The Z-average particle diameter of the complexes was <200 nm. The surface charge increased with increasing amount of chitosan. We observed that chitosan induces the base-stacking of miRNA in a concentration dependent manner. Surface plasmon resonance spectroscopy shows that complexes formed by low degree of acetylation chitosans are highly stable, regardless of the molecular weight. We found no evidence that these complexes were cytotoxic towards MCF-7 cells. Furthermore, CS–miRNA nanocomplexes with degree of acetylation 12% and 29% were biologically active, showing successful downregulation of target mRNA expression in MCF-7 cells. Our data, therefore, shows that CS–miRNA complexes offer a promising non-viral platform for breast cancer gene therapy

    Interplay between Structure and Dynamics in Chitosan Films Investigated with Solid-State NMR, Dynamic Mechanical Analysis, and X-ray Diffraction

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    Modern solid-state NMR techniques, combined with X-ray diffraction, revealed the molecular origin of the difference in mechanical properties of self-associated chitosan films. Films cast from acidic aqueous solutions were compared before and after neutralization, and the role of the counterion (acetate vs Cl⁻) was investigated. There is a competition between local structure and long-range order. Hydrogen bonding gives good mechanical strength to neutralized films, which lack long-range organization. The long-range structure is better defined in films cast from acidic solutions in which strong electrostatic interactions cause rotational distortion around the chitosan chains. Plasticization by acetate counterions enhances long-range molecular organization and film flexibility. In contrast, Cl⁻ counterions act as a defect and impair the long-range organization by immobilizing hydration water. Molecular motion and proton exchange are restricted, resulting in brittle films despite the high moisture content

    Rheological Study of Genipin Cross-Linked Chitosan Hydrogels

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    This paper reports the rheological behavior of chitosan solutions that have been cross-linked with different amounts of genipin, at body temperature and physiological pH. The effect of the cross-linker loading on the rheological properties of hydrogels has been evaluated. The oscillatory time sweep method was used to monitor the dynamic viscoelastic parameters during in situ (i.e., in the rheometer) gelation experiments, enabling the determination of the gelation time. The stress and frequency sweeps were employed to measure Gâ€Č of the cured hydrogels. It was found that the solutions of chitosan cross-linked with genipin, under physiological conditions, could form relatively strong elastic gels when compared to those of pure chitosan. Moreover, the gelation time obtained from the crossover of Gâ€Čâ€Č and Gâ€Č was in excellent agreement with the value obtained from the Winter–Chambon criterion. A significant reduction on this parameter was achieved even at low genipin concentrations. This behavior suggests that these formulations are able to be produced in situ and thus constitute promising matrices for cells and bioactive molecule encapsulations
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