Energy of Cohesion, Compressibility, and the Potential Energy Functions of the Graphite System

The lattice summations of the potential energy of importance in the graphite system have been computed by direct summation assuming a Lennard-Jones 6-12 potential between carbon atoms. From these summations, potential energy curves were constructed for interactions between a carbon atom and a graphite monolayer, between a carbon atom and a graphite surface, between a graphite monolayer and a semi-infinite graphite crystal and between two graphite semi-infinite crystals. Using these curves, the equilibrium distance between two isolated physically interacting carbon atoms was found to be 2.70 a, where a is the carbon-carbon distance in a graphite sheet. The distance between a surface plane and the rest of the crystal was found to be 1.7% greater than the interlayer spacing. Theoretical values of the energy of cohesion and the compressibility were calculated from the potential curve for the interaction between two semi-infinite crystals. They were (delta)E(sub c) = -330 ergs/sq cm and beta =3.18x10(exp -12)sq cm/dyne, respectively. These compared favorably with the experimental values of (delta)E(sub c) = -260 ergs/sq cm and beta = 2.97 X 10(exp -2) sq cm/dyne

Human Heme Oxygenase Oxidation of 5- and 15-Phenylhemes

Human heme oxygenase-1 (hHO-1) catalyzes the O2- dependent oxidation of heme to biliverdin, CO, and free iron. Previous work indicated that electrophilic addition of the terminal oxygen of the ferric hydroperoxo complex to the -meso-carbon gives 5-hydroxyheme. Earlier efforts to block this reaction with a 5-methyl substituent failed, as the reaction still gave biliverdin IX . Surprisingly, a 15-methyl substituent caused exclusive cleavage at the -meso- rather than at the normal, unsubstituted -meso-carbon. No CO was formed in these reactions, but the fragment cleaved from the porphyrin eluded identification. We report here that hHO-1 cleaves 5-phenylheme to biliverdin IX and oxidizes 15- phenylheme at the -meso position to give 10-phenylbiliverdin IX . The fragment extruded in the oxidation of 5-phenylheme is benzoic acid, one oxygen of which comes from O2 and the other from water. The 2.29- and 2.11-Å crystal structures of the hHO-1 complexes with 1- and 15-phenylheme, respectively, show clear electron density for both the 5- and 15-phenyl rings in both molecules of the asymmetric unit. The overall structure of 15-phenylheme-hHO-1 is similar to that of heme-hHO-1 except for small changes in distal residues 141–150 and in the proximal Lys18 and Lys22. In the 5-phenylhemehHO-1 structure, the phenyl-substituted heme occupies the same position as heme in the heme-HO-1 complex but the 5-phenyl substituent disrupts the rigid hydrophobic wall of residues Met34, Phe214, and residues 26–42 near the -meso carbon. The results provide independent support for an electrophilic oxidation mechanism and support a role for stereochemical control of the reaction regiospecificity.Fil: Wang, Jingling. University of California; Estados UnidosFil: Niemevz, Fernando. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Orgánica; ArgentinaFil: Lad, Latesh. University of California; Estados UnidosFil: Huang, Liusheng. University of California; Estados UnidosFil: Alvarez, Diego Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Orgánica; ArgentinaFil: Buldain, Graciela Yolanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Orgánica; ArgentinaFil: Poulos, Thomas L.. University of California; Estados UnidosFil: Ortiz de Montellano, Paul R.. University of California; Estados Unido

The Supposedly Subaltern Progressions: the example of Brazilian nursing

This is a study about women as health educators and public health nurses in the first half of the 20(th) century in Brazil. Historical sociology, as a methodological and theoretical tool, will guide our analysis of the relations among,institutions, professional power, and identities, highlighting the ways through which women professionals were capable-of creating their own territory of autonomous action. In Brazil, the configuration of the public health field in the early 1920S was intimately associated with nation-building processes, and demanded new professionals for traditionally female occupations. The training of the young candidates took peace at the Oswaldo Cruz Institute, in Rio de Janeiro, at Sao Paulo's Institute of Hygiene, at Medical Schools, in foreign centers such as the Teachers College and the Toronto School of Nursing, as well as by means of on-the-job training. The sociological literature has stressed the (basically male) medical dominance, to the detriment of a focus on-the new emerging professions. This trend has been clear in Brazil, and it is time that the "sociological gaze" took a close interest in the unique role played by women as health educators and visiting nurses, based in community health centers as early as 1925 in Sao Paulo, Rio de Janeiro and other centers, and in rural health units across the nation. Health education was the key element in this new scenario of campaigns against endemic diseases - a national practice which Brazilians called "campanhismo" - that stressed non-authoritarian means in place of old schemes of medical policy.172354

Bone Pain and Muscle Weakness in Cancer Patients

PURPOSE OF REVIEW: In this article, we will discuss the current understanding of bone pain and muscle weakness in cancer patients. We will describe the underlying physiology and mechanisms of cancer-induced bone pain (CIBP) and cancer-induced muscle wasting (CIMW), as well as current methods of diagnosis and treatment. We will discuss future therapies and research directions to help patients with these problems. RECENT FINDINGS: There are several pharmacologic therapies that are currently in preclinical and clinical testing that appear to be promising adjuncts to current CIBP and CIMW therapies. Such therapies include resiniferitoxin, which is a targeted inhibitor of noceciptive nerve fibers, and selective androgen receptor modulators, which show promise in increasing lean mass. CIBP and CIMW are significant causes of morbidity in affected patients. Current management is mostly palliative; however, targeted therapies are poised to revolutionize how these problems are treated

Quantitative traits for the tail suspension test: automation, optimization, and BXD RI mapping

Immobility in the tail suspension test (TST) is considered a model of despair in a stressful situation, and acute treatment with antidepressants reduces immobility. Inbred strains of mouse exhibit widely differing baseline levels of immobility in the TST and several quantitative trait loci (QTLs) have been nominated. The labor of manual scoring and various scoring criteria make obtaining robust data and comparisons across different laboratories problematic. Several studies have validated strain gauge and video analysis methods by comparison with manual scoring. We set out to find objective criteria for automated scoring parameters that maximize the biological information obtained, using a video tracking system on tapes of tail suspension tests of 24 lines of the BXD recombinant inbred panel and the progenitor strains C57BL/6J and DBA/2J. The maximum genetic effect size is captured using the highest time resolution and a low mobility threshold. Dissecting the trait further by comparing genetic association of multiple measures reveals good evidence for loci involved in immobility on chromosomes 4 and 15. These are best seen when using a high threshold for immobility, despite the overall better heritability at the lower threshold. A second trial of the test has greater duration of immobility and a completely different genetic profile. Frequency of mobility is also an independent phenotype, with a distal chromosome 1 locus

Synaptic and extrasynaptic NMDA receptors are gated by different endogenous coagonists.

N-methyl-D-aspartate receptors (NMDARs) are located in neuronal cell membranes at synaptic and extrasynaptic locations, where they are believed to mediate distinct physiological and pathological processes. Activation of NMDARs requires glutamate and a coagonist whose nature and impact on NMDAR physiology remain elusive. We report that synaptic and extrasynaptic NMDARs are gated by different endogenous coagonists, D-serine and glycine, respectively. The regionalized availability of the coagonists matches the preferential affinity of synaptic NMDARs for D-serine and extrasynaptic NMDARs for glycine. Furthermore, glycine and D-serine inhibit NMDAR surface trafficking in a subunit-dependent manner, which is likely to influence NMDARs subcellular location. Taking advantage of this coagonist segregation, we demonstrate that long-term potentiation and NMDA-induced neurotoxicity rely on synaptic NMDARs only. Conversely, long-term depression requires both synaptic and extrasynaptic receptors. Our observations provide key insights into the operating mode of NMDARs, emphasizing functional distinctions between synaptic and extrasynaptic NMDARs in brain physiology

IGERT: Sensor Science, Engineering, and Informatics

This Sensor Science, Engineering and Informatics (SSEI) IGERT program will provide multidisciplinary doctoral training in the area of sensor systems ranging from the science and engineering of new materials and sensing mechanisms to the interpretation of sensor data. The design and management of effective sensor systems requires a holistic understanding of how information is collected, stored, integrated, evaluated, and communicated within sensing systems and to decision makers in diverse application contexts. The SSEI IGERT weaves together three research focus areas: (1) Sensor Materials and Devices, (2) Sensor Systems and Networks, and (3) Sensor Informatics. The intellectual merit of the project includes education and research activities that are designed to ensure a feedback loop so that SSEI IGERT trainees are able to transform new knowledge from sensor-generated data to further development of sensor systems and networks and advances in sensor materials and devices, and vice versa. Innovative components of the program include (1) development and use of a testbed prototype that will require interdisciplinary interaction across the three research areas; (2) a tight integration of the social, legal, ethical, and economic dimensions of sensing environments in both research and training, (3) expanded relationships with companies and federal laboratories engaged in sensor research, (4) international collaborations, and (5) synergistic integration with sensor science and engineering education at the middle, high school, and undergraduate level. The broader impacts of the SSEI IGERT program are a new breed of scientists and engineers who will be versatile in dealing with the diverse technical components that contribute to sensing systems, knowledgeable in the legal, social, and ethical contexts of heavily sensed environments, and aware of the human values that must be preserved, protected and promoted within such systems. IGERT is an NSF-wide program intended to meet the challenges of educating U.S. Ph.D. scientists and engineers with the interdisciplinary background, deep knowledge in a chosen discipline, and the technical, professional, and personal skills needed for the career demands of the future. The program is intended to catalyze a cultural change in graduate education by establishing innovative new models for graduate education and training in a fertile environment for collaborative research that transcends traditional disciplinary boundaries

Probing ultrafast dynamics in a solid-density plasma created by an intense femtosecond laser

We report a study on the dynamics of a near-solid density plasma using an ultraviolet (266 nm) femtosecond probe laser pulse, which can penetrate to densities of ∼ 1022 cm-3, nearly an order of magnitude higher than the critical density of the 800 nm, femtosecond pump laser. Time-resolved probe-reflectivity from the plasma shows a rapid decay (picosecond- timescale) while the time-resolved reflected probe spectra show red shifts at early temporal delays and blue shifts at longer delays. This spectral behaviour of the reflected probe can be explained by a laser-driven shock moving inward and a subsequent hydrodynamic free expansion in the outward direction