252 research outputs found
Clasificación de cultivos y de sus medidas agroambientales mediante segmentación de imágenes QuickBird
En la últimas décadas han ido creciendo considerablemente
los conocimientos y la sensibilización
sobre la protección al medioambiente en
muy diversas áreas, entre las que se encuentra la
Agricultura. El uso intensivo del laboreo ocasiona
graves daños medioambientales como la
erosión del suelo, la contaminación de las aguas
superficiales (escorrentía y colmatación de embalses),
el descenso del contenido de la materia
orgánica y de la biodiversidad de los suelos labrados,
y el aumento de la emisión de CO2 del
suelo a la atmósfera. Actualmente, la Unión Europea
sólo subvenciona a los agricultores que
cumplen lo que se conoce como “Medidas Agroambientales
o de Condicionalidad” cuyo diseño
ha estado dentro de las competencias de las Políticas
Agrarias Autonómicas, Nacionales y Europeas.
Estas medidas consisten en alterar el
perfil y la estructura del suelo lo menos posible,
dejando éste sin labrar y permanentemente protegido
por cubiertas vegetales (rastrojo) en el
caso de cultivos herbáceos (ej. trigo, maíz, girasol),
o por cubiertas vegetales vivas o inertes
(restos de poda) en el caso de cultivos leñosos
(principalmente cítricos y olivar). El seguimiento
del cumplimiento de estas medidas se realiza a través de visitas presenciales a un 1% de
los campos susceptibles de recibir ayudas. Este
método es ineficiente y provoca muchos errores
con la consiguiente presentación de un ingente
número de reclamaciones. Para subsanar esta
problemática, en este artículo presentamos los resultados
obtenidos en la clasificación de los cultivos
y las medidas agroambientales asociadas a
éstos en una imagen multiespectral QuickBird tomada
a principios de Julio de una zona típica de
cultivos en régimen de secano de Andalucía. Se
aplicaron 5 métodos de clasificación (Paralelepípedos,
P; Mínima Distancia, MD; Distancia de
Mahalanobis, MC; Mapeo del Ángulo Espectral,
SAM; y Máxima Probabilidad, ML) para la discriminación
de rastrojo de trigo quemado y sin
quemar, arbolado, carreteras, olivar, cultivos herbáceos
de siembra primaveral y suelo desnudo.
Además, la imagen es segmentada en objetos
para comparar la fiabilidad obtenida aplicando
los métodos anteriores partiendo tanto de píxeles
como de objetos como Unidades Mínimas de
Información (MIU). El análisis de los resultados
permite concluir que las clasificaciones de todos
los usos de suelo basadas en objetos claramente
mejoraron las basadas en píxeles, obteniéndose
precisiones (overall accuracy) mayores al 85%.
La elección de un método de clasificación u otro
influye en gran medida en la precisión de los
mapas obtenidos.
Debido a que la precisión del mapa temático
que necesitamos obtener ha de ser muy elevada
para tomar decisiones sobre Conceder / No conceder
las ayudas, sería interesante estudiar si el
incremento de la resolución espacial que se obtenga
gracias a la fusión de imágenes multiespectral
y pancromática de QuickBird para
obtener una imagen fusionada con resolución espacial
de la pancromática (0.7 m) y espectral de
la multiespectral (4 bandas) mejora la precisión
de cualquiera de los métodos de clasificación estudiadosSoil management in crops is mainly based on
intensive tillage operations, which have a great
relevancy in terms of increase of atmospheric
CO2, desertification, erosion and land degradation.
Due to these negative environmental impacts,
the European Union only subsidizes
cropping systems which require the implementation
of certain no-tillage systems and agro-environmental
measures, such as keeping the
winter cereal residues and non-burning of stubble
to reduce erosion, and to increase the organic
matter, the fertility of soils and the crop production.
Nowadays, the follow-up of these agrarian
policy actions is achieved by ground visits to
sample targeted farms; however, this procedure is
time-consuming and very expensive. To improve
this control procedure, a study of the accuracy
performance of several classification methods
has been examined to verify if remote sensing
can offer the ability to efficiently identify crops
and their agro-environmental measures in a typical
agricultural Mediterranean area of dry conditions.
Five supervised classification methods
based on different decision rule routines, Parallelepiped
(P), Minimum Distance (MD), Mahalanobis
Classifier Distance (MC), Spectral Angle Mapper (SAM), and Maximum Likelihood
(ML), were examined to determine the most suitable
classification algorithm for the identification
of agro-environmental measures such as
winter cereal stubble and burnt stubble areas and
other land uses such as river side trees, vineyard,
olive orchards, spring sown crops, roads and bare
soil. An object segmentation of the satellite information
was also added to compare the accuracy
of the classification results of pixel and
object as Minimum Information Unit (MIU). A
multispectral QuickBird image taken in early
summer was used to test these MIU and classification
methods. The resulting classified images
indicated that object-based analyses clearly outperformed
pixel ones, yielding overall accuracies
higher than 85% in most of the classifications.
The choice of a classification method can markedly
influence the accuracy of classification
maps
Detection of hepatitis C virus RNA in saliva of patients with active infection not associated with periodontal or liver disease severity
BACKGROUND: Hepatitis C virus (HCV) is mainly transmitted by parenteral route, being blood transfusion and intravenous drug use the most frequent risk factors. However, it has been suggested that there are other routes of transmission. There are several studies where HCV RNA has been detected in saliva of patients infected with HCV, and epidemiological studies have proposed the dental treatments as possible risk factors for HCV transmission. The purpose of this study was to detect the presence of HCV RNA in saliva of patients with active infection and associating with periodontal or liver disease. METHODS: Patients with quantifiable HCV-RNA in serum were enrolled in the study. Periodontal disease was assessed using the modified gingival index (MGI). Presence of dental plaque was assessed with the use of disclosing tablets. Patients were clinically and laboratory evaluated to identify the stage of liver disease, the HCV RNA was determinate in saliva by nested RT-PCR. To determine associations between different parameters univariate and multivariate analysis were used. RESULTS: A total of 45 patients were included. Of these patients, 21 (46.6%) had hepatitis, 23 (51.1%) had cirrhosis and one patient (2.4%) presented hepatocellular carcinoma (HCC). Viral loads in serum ranged from 2.31–6.68 log IU/ml with a mean of 5.46 log IU/ml (95% CI 5.23–5.70). HCV RNA was positive in saliva of 29 patients (64.4%) and was not detected in 16 (35.6%). For univariate analysis three independent variables were associated with the detection of HCV-RNA in saliva: gender, viral load and dental plaque and multivariate analysis only one independent variable viral load >5.17 log IU/mL remained significantly associated with the detection of HCV in saliva (p = 0.0002). A statistical difference was observed when viral load was analyzed, log 5.85 IU/mL (95% CI 5.67–6.02) for patients with HCV in saliva vs. log 4.77 IU/mL (95% CI 4.35–5.19) for patients without HCV in saliva (p = 0.0001). The detection of HCV-RNA in saliva was more frequent in patients with relatively high serum viral loads. CONCLUSION: HCV-RNA in saliva was associated with the level of serum viral load but not with periodontal or liver disease severity
Aproximación Proteómica al síndrome de apneas-hipopneas del sueño
Comunicaciones a congreso
Consensus statement on the diagnosis, management, and treatment of angioedema mediated by Bradykinin. Part. II: treatment, follow-up, and special situations
Background: There are no previous Spanish guidelines or consensus statements on bradykinin-induced angioedema.
Aim: To draft a consensus statement on the management and treatment of angioedema mediated by bradykinin in light of currently available
scientifi c evidence and the experience of experts. This statement will serve as a guideline to health professionals.
Methods: The consensus was led by the Spanish Study Group on Bradykinin-Induced Angioedema, a working group of the Spanish Society
of Allergology and Clinical Immunology. A review was conducted of scientifi c papers on different types of bradykinin-induced angioedema
(hereditary and acquired angioedema due to C1 inhibitor defi ciency, hereditary angioedema related to estrogens, angioedema induced by
angiotensin-converting enzyme inhibitors). Several discussion meetings were held to reach the consensus.
Results: Treatment approaches are discussed, and the consensus reached is described. Specifi c situations are addressed, namely, pregnancy,
contraception, travelling, blood donation, and organ transplantation.
Conclusions: A review of and consensus on treatment of bradykinin-induced angioedema is presentedIntroducción: No existen guías previas españolas sobre el manejo del angioedema mediado por bradicinina.
Objetivos: Alcanzar un consenso sobre el manejo y tratamiento del angioedema mediado por bradicinina a la luz de la evidencia científi ca
disponible y la experiencia de los expertos, que sirva como guía para los profesionales de la salud.
Métodos: SGBA/GEAB, un grupo de trabajo de la SEAIC dirigió el consenso. Se realizó una revisión de los documentos científi cos publicados
sobre los diferentes tipos de angioedema mediado por bradicinina [angioedema hereditario o adquirido por defi ciencia de inhibidor de la
C1 esterasa, angioedema hereditario relacionado con estrógenos (AEH tipo III, AEH-FXII), angioedema inducido por IECA (inhibidores del
enzima convertidor de angiotensina]. Hubo varias reuniones del SGBA/GEAB para alcanzar el consenso.
Resultados: Se revisan y discuten los diferentes tratamientos disponibles y se describe el consenso alcanzado. Se abordan situaciones
específi cas (embarazo, anticoncepción, viajes, hemodonación, trasplante de órganos).
Conclusiones: Se presenta una revisión del tratamiento del angioedema mediado por bradicinina y un consenso sobre su tratamiento en
EspañaDr. Teresa Caballero is a researcher with the Hospital La Paz Health Research Institute (IdiPaz) program for promoting
research activities (2009
Study of Mechanical, Thermal, and Microstructural Properties of Polypropylene/Ceramic Waste Composites
In this work, the effect of reinforcement of the iPP with ceramic waste (CW), and the use of maleic anhydride compatibilizing agent grafted with polyolefin elastomer (POE-g-MAH) are studied. The composites were fabricated by extrusion and injection processes, and their morphology and microstructure, as well as fracture surface and mechanical and thermal properties were analyzed. Characterization by polarized optical microscopy showed that the ceramic waste particles were well-dispersed into the iPP matrix without the presence of agglomerates. However, the POE-g-MAH did not show good compatibility when it was added to the iPP/CW composite. Hardness Rockwell R, tensile and flexural measurements showed that the hardness, Young´s modulus, and flexural modulus increased with the incorporation of CW and without the POE-g-MAH. The ductility of the composites was several decreased with the addition of CW. POE-g-MAH affected the hardness, ductility, strength tensile, Young´s modulus, flexural modulus, and interfacial interaction in the iPP/CW composite. Analysis by X-ray diffraction showed that the CW also acted as a nucleating agent, increasing the crystallization degree, and forming the β-phase. Analysis of the Fourier transformed infrared showed transmittance bands of the iPP, CW, POE-g-MAH and composites. The bands were similar and there were no major changes in characteristic bands of composites, but CW and POE-g-MAH produced changes in the shape and intensity of band peaks of the iPP matrix. The CW addition to the iPP matrix modified the thermal properties of pure iPP, such as the degree of crystallization and melting temperature in the iPP/CW composites. The incorporation of POE-g-MAH decreased the crystallization temperature and crystallinity degree in the iPP/CW composite
Polymorphisms in host immunity modulating genes and risk of invasive aspergillosis: results from the aspBIOmics consortium
Recent studies suggest that immune-modulating single nucleotide polymorphisms (SNPs) influence the risk of developing cancer-related infections. Here, we evaluated whether 36 SNPs within 14 immune-related genes are associated with the risk of Invasive Aspergillosis (IA) and whether genotyping of these variants might improve disease risk prediction. We conducted a case-control association study of 781 immunocompromised patients, 149 of whom were diagnosed with IA. Association analysis showed that the IL4Rrs2107356 and IL8rs2227307 SNPs were associated with an increased risk of IA (OR=1.92, 95%CI: 1.20-3.09 and OR=1.73, 1.06-2.81) whereas the IL12Brs3212227 and IFN?rs2069705 variants were significantly associated with a decreased risk of developing the infection (OR=0.60, 0.38-0.96 and OR=0.63, 0.41-0.97). An allogeneic hematopoietic stem cell transplantation (allo-HSCT)-stratified analysis revealed that the effect observed for the IL4Rrs2107356 and IFN?rs2069705 SNPs was stronger in allo-HSCT (OR=5.63, 1.20-3.09 and OR=0.24, 0.10-0.59) than in non-HSCT patients, suggesting that the presence of these SNPs may render patients more vulnerable to infection especially under severe and prolonged immunosuppressive conditions. Importantly, in vitro studies revealed that carriers of the IFN?rs2069705C allele showed a significantly increased macrophage-mediated neutralisation of fungal conidia (P=0.0003) and, under stimulation conditions, produced higher levels of IFN? mRNA (P=0.049) and IFN? and TNFa cytokines (PLPS-96h=0.057, PPHA-96h=0.036 and PLPS+PHA-96h=0.030 and PPHA -72h=0.045, PLPS+PHA-72h=0.018, PLPS-96h=0.058 and PLPS+PHA -96h=0.0058, respectively). Finally, we also observed that the addition of SNPs significantly associated with IA to a model including clinical variables led to a substantial improvement in the discriminatory ability to predict the disease (AUC=0.659 vs. AUC=0.564, PLR=5.2•10-4 and P50.000Perm=9.34•10-5). These findings suggest that the IFN?rs2069705 SNP influences the risk of IA and that predictive models built with IFN?, IL8, IL12p70 and VEGFa variants might be used to predict disease risk and to implement risk-adapted prophylaxis or diagnostic strategies.This study was supported by grants PI12/02688 from the Fondo de Investigaciones Sanitarias (Madrid, Spain), PIM2010EPA-00756 from the ERA-NET PathoGenoMics (0315900A), and the Collaborative Research Center/Transregio 124 FungiNet. C.C. is supported by the Fundação para a Ciência e Tecnologia, Portugal (SFRH/BPD/96176/2013). This study also was supported by a donation of Consuelo González Moreno, an acute myeloid leukemia survivor. We thank Astella Pharma Inc. for supporting this work.info:eu-repo/semantics/publishedVersio
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Spatial Scale, Means and Gradients of Hydrographic Variables Define Pelagic Seascapes of Bluefin and Bullet Tuna Spawning Distribution
Seascape ecology is an emerging discipline focused on understanding how features of the marine habitat influence the
spatial distribution of marine species. However, there is still a gap in the development of concepts and techniques for its
application in the marine pelagic realm, where there are no clear boundaries delimitating habitats. Here we demonstrate
that pelagic seascape metrics defined as a combination of hydrographic variables and their spatial gradients calculated at
an appropriate spatial scale, improve our ability to model pelagic fish distribution. We apply the analysis to study the
spawning locations of two tuna species: Atlantic bluefin and bullet tuna. These two species represent a gradient in life
history strategies. Bluefin tuna has a large body size and is a long-distant migrant, while bullet tuna has a small body size
and lives year-round in coastal waters within the Mediterranean Sea. The results show that the models performance
incorporating the proposed seascape metrics increases significantly when compared with models that do not consider
these metrics. This improvement is more important for Atlantic bluefin, whose spawning ecology is dependent on the local
oceanographic scenario, than it is for bullet tuna, which is less influenced by the hydrographic conditions. Our study
advances our understanding of how species perceive their habitat and confirms that the spatial scale at which the seascape
metrics provide information is related to the spawning ecology and life history strategy of each species
Isolation of human fibroadipogenic progenitors and satellite cells from frozen muscle biopsies
Altres ajuts: Association Française contre les Myopathies (22525)Altres ajuts: Fundación Isabel GemioSkeletal muscle contains multiple cell types that work together to maintain tissue homeostasis. Among these, satellite cells (SC) and fibroadipogenic progenitors cells (FAPs) are the two main stem cell pools. Studies of these cells using animal models have shown the importance of interactions between these cells in repair of healthy muscle, and degeneration of dystrophic muscle. Due to the unavailability of fresh patient muscle biopsies, similar analysis of interactions between human FAPs and SCs is limited especially among the muscular dystrophy patients. To address this issue here we describe a method that allows the use of frozen human skeletal muscle biopsies to simultaneously isolate and grow SCs and FAPs from healthy or dystrophic patients. We show that while the purified SCs differentiate into mature myotubes, purified FAPs can differentiate into adipocytes or fibroblasts demonstrating their multipotency. We find that these FAPs can be immortalized and the immortalized FAPs (iFAPs) retain their multipotency. These approaches open the door for carrying out personalized analysis of patient FAPs and interactions with the SCs that lead to muscle loss
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