(2S,4aR,6aR,7R,9S,10aS,10bR)-7-Carb­oxy-2-(3-fur­yl)-6a,10b-dimethyl-4,10-dioxoperhydro­benzo[f]isochromen-9-yl acetate

The asymmetric unit of the title compound, C22H26O8, contains two crystallographically independent mol­ecules with closely comparable conformations (r.m.s. overlay = 0.54 Å for 30 non-H atoms). All six-membered rings display chair conformations, with a slight distortion for the lactone ring. The mol­ecules are connected by O—H⋯O hydrogen bonds into chains along [010], with the independent mol­ecules segregated into separate chains. The two mol­ecules in the asymmetric unit face each other in a head-to-tail fashion, with the furan ring of one mol­ecule turned towards the carboxylic acid terminal of the other mol­ecule

How to Write a Business Plan for an Independent, Narrative, Feature Film : Case: "Get Up, Get Out”

The author of the thesis has worked in film industry in the United States in various positions since his graduation from the Stella Adler Studio of Acting, New York, in 2012. Researching business planning in independent feature filmmaking was a natural extension to what the author already does in his professional life, and the commissioned work was a perfect match for the thesis. The purpose of the thesis was to find out how to write a business plan for an independent feature film to be produced in the Unites States, and whether business planning in independent feature filmmaking in the United States was a common practice. The author took a deductive approach, using both QUAL and QUAN research methods, and used case study as his strategy. Data was primarily collected via conducting a survey that was sent out to 250 independent feature film producers based in the United States. Secondary data was collected from literature and online sources, as per the reference list. Based on both the survey and secondary sources, the author concluded that business planning is crucial and widely used in independent feature filmmaking in the United States – especially when the main funding source is other than the filmmaker him/herself. As a by-product, as commissioned by tinygiant (“the Commissioner”), the author has created a comprehensive business plan for the case film Get Up, Get Out. The business plan was written using leading industry literature and online sources as guidelines. The business plan will be used as a tool to attract investors to finance the film that has an estimated production cost (“negative cost”) of $1.5MM. The business plan will be classified, and not available to the public, as per the commissioner's request. For further studies, the author recommends using a much larger focus group when conducting a questionnaire, as the response rate was only 4%. The author would find further studies on independent film profitability correlating with the quality of business planning very interesting and useful. However, these findings are out of the scope of this thesis.The actual commissioner work, a business plan for "Get Up, Get Out" will not be publicized, as per the commissioner's request

Stanowisko prawne Wolnego Miasta Gdańska

Digitalizacja i deponowanie archiwalnych zeszytów RPEiS sfinansowane przez MNiSW w ramach realizacji umowy nr 541/P-DUN/201

In vitro Studies on Metabolism of Salvinorin A

Microbial transformation of natural products is a well established model for mammalian metabolism. Salvinorin A, a diterpenoid isolated from the hallucinogenic mint Salvia divinorum Epling & Játiva-M (Lamiaceae), is a potent non-nitrogenous κ-opioid receptor agonist. The metabolism of salvinorin A has still not yet been well established. Thirty fungal species were screened for the ability to metabolize salvinorin A. We observed that salvinorin A undergoes fast hydrolysis of the acetate group at carbon atom C2, resulting in formation of the pharmacologically inactive product, salvinorin B. Ex vivo experiments were also performed using organelle fractions isolated from rat liver and brain. Crude tissue homogenate and individual organelles show that the primary route of salvinorin A metabolism is hydrolysis to salvinorin B. No metabolic transformation of salvinorin B was observed in these studies

Expanding the landscape of diterpene structural diversity by stereochemically controlled combinatorial biosynthesis

Plant‐derived diterpenoids serve as important pharmaceuticals, food additives, and fragrances, yet their low natural abundance and high structural complexity limits their broader industrial utilization. By mimicking the modularity of diterpene biosynthesis in plants, we constructed 51 functional combinations of class I and II diterpene synthases, 41 of which are “new‐to‐nature”. Stereoselective biosynthesis of over 50 diterpene skeletons was demonstrated, including natural variants and novel enantiomeric or diastereomeric counterparts. Scalable biotechnological production for four industrially relevant targets was accomplished in engineered strains of Saccharomyces cerevisiae