Quantitative characteristics of rainbow trout (Oncorhynchus mykiss) semen throughout the reproductive season

The aim of this study was to determine changes in quantitative characteristics of the rainbow trout semen throughout the reproductive season. Semen samples were collected from October to May from broodstock males (n = 12) kept under natural photoperiod regime and semen volume, density, motility, duration of motility, spermatocrit and pH parameters were investigated. In October, one of the 12 brooders (8.3%) were spermiating, increasing to 12 (100%) in December and dropping to 3 (25%) in May. Semen volume significantly increased in January and February. The total volume of expressible semen was maximal in January, increasing from a mean value of 1.7 ml to 20.9 ml. The spermatozoa density showed an increasing trend from October to January, with mean values ranging between 1.4 and 10.3 x 109 sperm/ml. The spermatocrit did not vary with the sampling date. The semen samples collected in November showed the lowest motility of spermatozoa (40%). But the percentage of motile spermatozoa collected from January to May showed more than 80% motility. The duration of motility for the monitoring period ranged between 58.0 and 375.6 sec. In conclusion, the highest quality semen was collected during the medial part of the reproductive season.Bu çalışmanın amacı üreme mevsimi boyunca gökkuşağı alabalığı spermasının nicel özelliklerindeki değişimleri belirlemektir. Sperma örnekleri doğal fotoperiyot altında tutulan damızlıklardan (n = 12) ekim kasım ayları arasında toplandı. Sperma miktarı ve pH, sperma yoğunluğu ve motilite, motil kalma süresi, spermatokrit belirlendi. Ekimde 12 damızlıktan bir tanesi (%8.3) sperm verirken, aralık ayında sperm veren birey sayısı 12’ye (%100) yükseldi ve mayıs ayında 3 (%3) bireye düştü. Sperma miktarı ocak ve şubat aylarında önemli ölçüde arttı. Sağılabilen sperma miktarı, 1.7 ml’den 20.9 ml’ye yükselerek, ocak ayında en yüksek düzeye çıktı. Ekimde 1.4 x 109 sperm/ml olan spermatozoa yoğunluğu ocak ayında 10.3 x 109 sperm/ml olarak saptandı. Spermatokrit değerleri örnekleme zamanına bağlı olarak değişim göstermedi. En düşük motilite kasımdaki örneklerde, ocak ve mayıs arasında toplanan örneklerde ise %80’den yüskek motilite saptandı. Motil kalma süresi, üreme mevsimi boyunca, 58.0 ve 375.6 s arasında değişti. Sonuç olarak, yüksek kalitede sperma üreme mevsimi ortasında toplandı

Unique and conserved MicroRNAs in wheat chromosome 5D revealed by next-generation sequencing

MicroRNAs are a class of short, non-coding, single-stranded RNAs that act as post-transcriptional regulators in gene expression. miRNA analysis of Triticum aestivum chromosome 5D was performed on 454 GS FLX Titanium sequences of flow sorted chromosome 5D with a total of 3,208,630 good quality reads representing 1.34x and 1.61x coverage of the short (5DS) and long (5DL) arms of the chromosome respectively. In silico and structural analyses revealed a total of 55 miRNAs; 48 and 42 miRNAs were found to be present on 5DL and 5DS respectively, of which 35 were common to both chromosome arms, while 13 miRNAs were specific to 5DL and 7 miRNAs were specific to 5DS. In total, 14 of the predicted miRNAs were identified in wheat for the first time. Representation (the copy number of each miRNA) was also found to be higher in 5DL (1,949) compared to 5DS (1,191). Targets were predicted for each miRNA, while expression analysis gave evidence of expression for 6 out of 55 miRNAs. Occurrences of the same miRNAs were also found in Brachypodium distachyon and Oryza sativa genome sequences to identify syntenic miRNA coding sequences. Based on this analysis, two other miRNAs: miR1133 and miR167 were detected in B. distachyon syntenic region of wheat 5DS. Five of the predicted miRNA coding regions (miR6220, miR5070, miR169, miR5085, miR2118) were experimentally verified to be located to the 5D chromosome and three of them : miR2118, miR169 and miR5085, were shown to be 5D specific. Furthermore miR2118 was shown to be expressed in Chinese Spring adult leaves. miRNA genes identified in this study will expand our understanding of gene regulation in bread wheat

Teaching the Academic Word List in Foreign Language Speaking Classes

WOS: 00043485270039

Effects of hospital facilities on patient outcomes after cancer surgery: an international, prospective, observational study

Background Early death after cancer surgery is higher in low-income and middle-income countries (LMICs) compared with in high-income countries, yet the impact of facility characteristics on early postoperative outcomes is unknown. The aim of this study was to examine the association between hospital infrastructure, resource availability, and processes on early outcomes after cancer surgery worldwide.Methods A multimethods analysis was performed as part of the GlobalSurg 3 study-a multicentre, international, prospective cohort study of patients who had surgery for breast, colorectal, or gastric cancer. The primary outcomes were 30-day mortality and 30-day major complication rates. Potentially beneficial hospital facilities were identified by variable selection to select those associated with 30-day mortality. Adjusted outcomes were determined using generalised estimating equations to account for patient characteristics and country-income group, with population stratification by hospital.Findings Between April 1, 2018, and April 23, 2019, facility-level data were collected for 9685 patients across 238 hospitals in 66 countries (91 hospitals in 20 high-income countries; 57 hospitals in 19 upper-middle-income countries; and 90 hospitals in 27 low-income to lower-middle-income countries). The availability of five hospital facilities was inversely associated with mortality: ultrasound, CT scanner, critical care unit, opioid analgesia, and oncologist. After adjustment for case-mix and country income group, hospitals with three or fewer of these facilities (62 hospitals, 1294 patients) had higher mortality compared with those with four or five (adjusted odds ratio [OR] 3.85 [95% CI 2.58-5.75]; p<0.0001), with excess mortality predominantly explained by a limited capacity to rescue following the development of major complications (63.0% vs 82.7%; OR 0.35 [0.23-0.53]; p<0.0001). Across LMICs, improvements in hospital facilities would prevent one to three deaths for every 100 patients undergoing surgery for cancer.Interpretation Hospitals with higher levels of infrastructure and resources have better outcomes after cancer surgery, independent of country income. Without urgent strengthening of hospital infrastructure and resources, the reductions in cancer-associated mortality associated with improved access will not be realised

Metal Ceramic Bond After Multiple Castings of Base Metal Alloy

PubMedID: 19703623[No abstract available

Sewability properties of lining fabrics

The purpose of a lining is to provide the comfort of the wearer, preserve the shape of a garment, conceal construction details and raw edges of fabric, thus giving a neat appearance to the inside of the garment. In this study, it was aimed to research the sewability properties of main lining fabrics by using the L&M Sewability Tester. Six types of linings were used and they were grouped according to their warp and weft densities, yarn count and fabric composition. Three groups were generated by considering variations of the warp density, weft density or yarn count, while the other parameters were constant. At the end of the study, it has seen that, yarn count and density have a direct influence on the sewability values of lining fabrics. Also it was observed that sewing needle penetration force of lining fabrics were affected by the fabric density in both directions and yarn count

Bacterial pathogens and health problems of Acipenser gueldenstaedtii and Acipenser baerii sturgeons reared in the eastern Black Sea region of Turkey

KURTOGLU, ILKER ZEKI/0000-0002-4214-7997WOS: 000404612800004PubMed: 28580011Farming sturgeons is an economically important practice in a number of Asian and European countries. However, since it is not widely implementedin Turkey, fertilized eggs necessary for research and industrial activities are imported from Germany. Due to the interest of several fish farms in culturing sturgeon in Turkey and the lack of relevant data, this study investigated bacteria related health problems of two different sturgeon species, the diamond sturgeon (Acipenser gueldenstaedtii) and the Siberian sturgeon (Acipenser baerii). the fungal, parasitic and bacterial pathogens found in these fish were investigated until the fish reached about 3 kg of weight (3+ years). A number of bacterial disease pathogens (Acinetobacter radioresistens, some Aeromonas and Pseudomonas species and Bacillus mycoides) and parasite Trichodina sp. and fungus Saprolegnia sp. were identified in the fish. Both phenotypic and molecular characterizations of the isolated bacteria were performed. Furthermore, swim bladder and spinal problems, cannibalism, tumor growth and mechanical injuries on the external surface of the fish were observed during the study period.Recep Tayyip Erdogan University Research Project FundRecep Tayyip Erdogan University [2011.103.02.3]The study was funded by Recep Tayyip Erdogan University Research Project Fund (Project number; 2011.103.02.3)