Effects of Hybrid Poplar (Salicaceae) Clone and Phenology on Gypsy Moth (Lepidoptera: Lymantriidae) Performance in Wisconsin

Gypsy moth (Lymantria dispar) developmental interactions with two hybrid Populus species clones were studied in laboratory trials. Significant differences in larval performance were found between clones and within the same clone at different phenological states. No larvae were able to complete development on clone NM6. All gypsy moth larvae feeding on clone NC5271 survived when leafflush was synchronized with gypsy moth eclosion in early May. However, neonates feeding on NC5271 foliage in July experienced in- creased mortality. Weights of surviving gypsy moth larvae feeding on NC5271 foliage in May versus July were not significantly different

Prescribed Burning Guidelines in the Northern Great Plains

The use of fire to manage grasslands for wildlife is a relatively new management option for resource managers in the Northern Great Plains (NGP). Nearly all of the burning during the past 20-25 years has been conducted without the aid of specific guidelines for the region. This state-of-the-art set of recommendations was compiled because of this void. Records of 902 grassland fires (primarily on U.S. Fish and Wildlife lands), personal experiences, and synopses of other published fire research were used in developing the guidelines in this manual. Fifty-two percent of the 902 fires were in native prairie grasslands with lesser amounts in tame and native grass plantings, wetlands, and woodlands. Prescription grassland fires averaged 31 ha (77 acres) per burn. The personnel needed to safely conduct a grassland fire depended on the size of the burn, the kind of firebreaks, available equipment, and weather conditions. Costs and hours of effort to conduct fires were inversely related to burn area size. Cost ratios are extremely high for fires of less than 4 ha (10 acres). They are essentially the same for burns of 16 to 113 ha (40 to 280 acres). The two primary reasons for burning grasslands are wildlife habitat improvement and native prairie restoration. Fire use steadily increased between 1965 and 1984, but the greatest increase occurred following workshop instruction in 1978. These guidelines present a set of reasons, criteria, techniques, and examples of simple prescriptions which aid in the planning and execution of a safe and effective prescribed burning program for wildlife enhancement in grassland areas of the NGP

Effects of Fire in the Northern Great Plains

This publication is a review of selected literature about prescribed burning in the Northern Great Plains for management of wildlife. It discusses the effect of fire on soil nutrients and minerals, upland grasses and forbs, undesirable species, shrubs, trees, certain plant species, emergent vegetation in prairie wetlands, insects, nongame birds, upland game birds, waterfowl, shorebirds, small mammals, and livestock

Effects of Fire in the Northern Great Plains

Fire has been used inconsistently to manage native and tame grasslands in the Northern Great Plains (NGP) of the north-central U.S. and south-central Canada, particularly the grasslands found in prairies, plains, agricultural land retirement programs, and moist soil sites. This has happened for three primary reasons: (1) the reduction of American Indian use of fire after 1875, (2) fire suppression and land use changes that put increasingly more acres under annual tillage since about the same time, and (3) a growing resistance to the use of fire since about 1940, largely due to media overemphasis of its harmful effects (e.g., Bambi and Smokey the Bear ). Little can be done to change the first two factors but there is ample opportunity to change human attitudes about fire. Attitudes change when the knowledge (or lack of it) changes. We believe that people have been reluctant to include fire in resource management programs in the NGP because of a lack of adequate information about the effects of fire on the soils, plants, and animals in the region. This document provides information concerning fire effects on the grassland biome of the NGP, with special emphasis on the use of fire for wildlife management. In several instances we have drawn from published literature outside the geographic region, but only to provide a more complete reference for readers and decision makers. In most instances, we only state or abstract the published findings of others without interpretation, either pro or con. Readers can fit the information into their specific circumstances. English and scientific names are from Flora of the Great Plains by the Great Plains Flora Association and from the Checklist of Vertebrates of the United States, the U.S. territories, and Canada by RC. Banks, R.W. McDiarmid, and A.L. Gardner

Prescribed Burning Guidelines in the Northern Great Plains

This publication provides guidelines with reasons, criteria, techniques, and examples of simple prescriptions which aid in the planning and execution of a safe and effective prescribed burning program for wildlife enhancement in grassland areas of the Northern Great Plains

Annotated Bibliography of Fire Literature

Natural resource managers have greatly increased the use of fire to manage grassland habitats during the past two decades in the northern Great Plains region of the United States and Canada. In support of these efforts, we have compiled this annotated bibliography to provide a condensed reference of fire literature for those managers with an interest in fire ecology. References are arranged alphabetically by author and year, numbered consecutively, and referenced by number in the author and subject indexes that follow the bibliography. The intent in compiling the bibliography and indexes is more to identify subject matter and to direct the reader to sources rather than to provide a digested interpretation of each manuscript

Evaluation of polygenic risk scores for breast and ovarian cancer risk prediction in BRCA1 and BRCA2 mutation carriers

Background: Genome-wide association studies (GWAS) have identified 94 common single-nucleotide polymorphisms (SNPs) associated with breast cancer (BC) risk and 18 associated with ovarian cancer (OC) risk. Several of these are also associated with risk of BC or OC for women who carry a pathogenic mutation in the high-risk BC and OC genes BRCA1 or BRCA2. The combined effects of these variants on BC or OC risk for BRCA1 and BRCA2 mutation carriers have not yet been assessed while their clinical management could benefit from improved personalized risk estimates. Methods: We constructed polygenic risk scores (PRS) using BC and OC susceptibility SNPs identified through population-based GWAS: for BC (overall, estrogen receptor [ER]-positive, and ER-negative) and for OC. Using data from 15 252 female BRCA1 and 8211 BRCA2 carriers, the association of each PRS with BC or OC risk was evaluated using a weighted cohort approach, with time to diagnosis as the outcome and estimation of the hazard ratios (HRs) per standard deviation increase in the PRS. Results: The PRS for ER-negative BC displayed the strongest association with BC risk in BRCA1 carriers (HR = 1.27, 95% confidence interval [CI] = 1.23 to 1.31, P = 8.2 x 10(53)). In BRCA2 carriers, the strongest association with BC risk was seen for the overall BC PRS (HR = 1.22, 95% CI = 1.17 to 1.28, P = 7.2 x 10(-20)). The OC PRS was strongly associated with OC risk for both BRCA1 and BRCA2 carriers. These translate to differences in absolute risks (more than 10% in each case) between the top and bottom deciles of the PRS distribution; for example, the OC risk was 6% by age 80 years for BRCA2 carriers at the 10th percentile of the OC PRS compared with 19% risk for those at the 90th percentile of PRS. Conclusions: BC and OC PRS are predictive of cancer risk in BRCA1 and BRCA2 carriers. Incorporation of the PRS into risk prediction models has promise to better inform decisions on cancer risk management

Combined systems approaches reveal highly plastic responses to antimicrobial peptide challenge in Escherichia coli

Obtaining an in-depth understanding of the arms races between peptides comprising the innate immune response and bacterial pathogens is of fundamental interest and will inform the development of new antibacterial therapeutics. We investigated whether a whole organism view of antimicrobial peptide (AMP) challenge on Escherichia coli would provide a suitably sophisticated bacterial perspective on AMP mechanism of action. Selecting structurally and physically related AMPs but with expected differences in bactericidal strategy, we monitored changes in bacterial metabolomes, morphological features and gene expression following AMP challenge at sub-lethal concentrations. For each technique, the vast majority of changes were specific to each AMP, with such a plastic response indicating E. coli is highly capable of discriminating between specific antibiotic challenges. Analysis of the ontological profiles generated from the transcriptomic analyses suggests this approach can accurately predict the antibacterial mode of action, providing a fresh, novel perspective for previous functional and biophysical studies

Breast and Prostate Cancer Risks for Male BRCA1 and BRCA2 Pathogenic Variant Carriers Using Polygenic Risk Scores

Background: Recent population-based female breast cancer and prostate cancer polygenic risk scores (PRS) have been developed. We assessed the associations of these PRS with breast and prostate cancer risks for male BRCA1 and BRCA2 pathogenic variant carriers. Methods: 483 BRCA1 and 1318 BRCA2 European ancestry male carriers were available from the Consortium of Investigators of Modifiers of BRCA1/2 (CIMBA). A 147-single nucleotide polymorphism (SNP) prostate cancer PRS (PRSPC) and a 313-SNP breast cancer PRS were evaluated. There were 3 versions of the breast cancer PRS, optimized to predict overall (PRSBC), estrogen receptor (ER)-negative (PRSER-), or ER-positive (PRSER+) breast cancer risk. Results: PRSER+ yielded the strongest association with breast cancer risk. The odds ratios (ORs) per PRSER+ standard deviation estimates were 1.40 (95% confidence interval [CI] =1.07 to 1.83) for BRCA1 and 1.33 (95% CI = 1.16 to 1.52) for BRCA2 carriers. PRSPC was associated with prostate cancer risk for BRCA1 (OR = 1.73, 95% CI = 1.28 to 2.33) and BRCA2 (OR = 1.60, 95% CI = 1.34 to 1.91) carriers. The estimated breast cancer odds ratios were larger after adjusting for female relative breast cancer family history. By age 85 years, for BRCA2 carriers, the breast cancer risk varied from 7.7% to 18.4% and prostate cancer risk from 34.1% to 87.6% between the 5th and 95th percentiles of the PRS distributions. Conclusions: Population-based prostate and female breast cancer PRS are associated with a wide range of absolute breast and prostate cancer risks for male BRCA1 and BRCA2 carriers. These findings warrant further investigation aimed at providing personalized cancer risks for male carriers and informing clinical management.Peer reviewe

Identification of 12 new susceptibility loci for different histotypes of epithelial ovarian cancer.

To identify common alleles associated with different histotypes of epithelial ovarian cancer (EOC), we pooled data from multiple genome-wide genotyping projects totaling 25,509 EOC cases and 40,941 controls. We identified nine new susceptibility loci for different EOC histotypes: six for serous EOC histotypes (3q28, 4q32.3, 8q21.11, 10q24.33, 18q11.2 and 22q12.1), two for mucinous EOC (3q22.3 and 9q31.1) and one for endometrioid EOC (5q12.3). We then performed meta-analysis on the results for high-grade serous ovarian cancer with the results from analysis of 31,448 BRCA1 and BRCA2 mutation carriers, including 3,887 mutation carriers with EOC. This identified three additional susceptibility loci at 2q13, 8q24.1 and 12q24.31. Integrated analyses of genes and regulatory biofeatures at each locus predicted candidate susceptibility genes, including OBFC1, a new candidate susceptibility gene for low-grade and borderline serous EOC