On the negative hydrogen ion and its absorption coefficient

The continuous absorption coefficient of the negative hydrogen ion is discussed from the point of view of the sum rules, and it is shown that the absorption coefficient to the red of 5000 A is predominantly governed by the wave-function of the ground state of H- at distances of the order of five times the Bohr radius from the center. It appears that the wave-functions for H- now in use are not sufficiently accurate at these distances to provide reliable values for the absorption coefficient beyond 5000 A, Further, some objective criteria are stated which should enable the reliability of a given absorption curve to be tested

Weaving of UML sequence diagrams using STAIRS

In this report we explore aspect-oriented modeling for UML 2.0 sequence diagrams. We ensure that the aspect weaving is semantics-based by using a formal trace model for sequence diagrams. A major challenge is to handle unbounded loops which produce infinite traces. We establish a systematic way to permutate and rewrite the original loop definition so that the weaving in many typical cases can be performed on a finite structure. We prove that it is always sufficient to consider a loop with upper bound relative to the pointcut definition to discover if the loop has infinitely repeating matches. A running example illustrates the approach and a prototype weaving tool is being implemented

Proteinase-activated receptor-2: two potential inflammatory mediators of the gastrointestinal tract in Atlantic salmon

Proteinase-activated receptor 2 (PAR-2), activated by trypsin and other serine proteinases, is a key initiator of inflammatory responses in the intestine of mammals. Atlantic salmon fed diets with standard qualities of soybean meal (SBM) show enteritis of the distal intestine as well as increased activity of trypsin in both luminal contents and wall tissue. Luminal trypsin activity may possibly be involved in immune related disorders of the intestine also in Atlantic salmon via activation of PAR 2. In the present study our aim was to investigate if PAR-2 play a role in SBM induced enteritis. We performed multiple alignments based on nucleic acid sequences of PAR-2 from various animals available from public databases, and designed primers for use in cloning of the Atlantic salmon PAR-2 transcript. We further cloned and characterized the full length sequence of Atlantic salmon PAR-2 and investigated the expression in both early and chronic stages of SBM induced enteropathy. Two full length versions of PAR-2 cDNA were identified and termed PAR-2a and PAR-2b. Expression of the two PAR-2 transcripts was detected in all 18 tissues examined, but most extensively in the intestine and gills. A significant up-regulation in the distal intestine was observed for the PAR-2a transcript after 1 day feeding diets containing SBM. After 3 weeks of feeding, PAR-2a was down-regulated compared to the fish fed control diets. These findings may indicate that PAR-2a participates in inflammatory responses in both the early and later stages of the SBM enteropathy. In the chronic stages of the enteropathy, down-regulation of PAR-2a may indicate a possible desensitization of the PAR-2a receptor. Expression of PAR-2b was not altered in the first 7 days of SBM feeding, but a significant up regulation was observed after 3 weeks, suggesting a putative role in chronic stages of SBM induced enteritis. The expression differences of the two PAR-2 transcripts in the feed trials may indicate that they have different roles in the SBM induced enteritis

Consistent changes in the intestinal microbiota of Atlantic salmon fed insect meal diets

Background Being part of fish's natural diets, insects have become a practical alternative feed ingredient for aquaculture. While nutritional values of insects have been extensively studied in various fish species, their impact on the fish microbiota remains to be fully explored. In an 8-week freshwater feeding trial, Atlantic salmon (Salmo salar) were fed either a commercially relevant reference diet or an insect meal diet wherein black soldier fly (Hermetia illucens) larvae meal comprised 60% of total ingredients. Microbiota of digesta and mucosa origin from the proximal and distal intestine were collected and profiled along with feed and water samples. Results The insect meal diet markedly modulated the salmon intestinal microbiota. Salmon fed the insect meal diet showed similar or lower alpha-diversity indices in the digesta but higher alpha-diversity indices in the mucosa. A group of bacterial genera, dominated by members of the Bacillaceae family, was enriched in salmon fed the insect meal diet, which confirms our previous findings in a seawater feeding trial. We also found that microbiota in the intestine closely resembled that of the feeds but was distinct from the water microbiota. Notably, bacterial genera associated with the diet effects were also present in the feeds. Conclusions We conclude that salmon fed the insect meal diets show consistent changes in the intestinal microbiota. The next challenge is to evaluate the extent to which these alterations are attributable to feed microbiota and dietary nutrients, and what these changes mean for fish physiology and health.publishedVersio

A comprehensive transcriptional body map of Atlantic salmon unveils the vital role of the intestine in the immune system and highlights functional specialization within its compartments

The intestine is a barrier organ that plays an important role in the immune system of Atlantic salmon. The immune functions are distributed among the diffuse gut lymphoid tissue containing diverse immune cells, and other cell types. Comparison of intestinal transcriptomes with those of other organs and tissues offers an opportunity to elucidate the specific roles of the intestine and its relationship with other parts of the body. In this work, a meta-analysis was performed on a large volume of data obtained using a genome-wide DNA oligonucleotide microarray. The intestine ranks third by the expression level of immune genes after the spleen and head kidney. The activity of antigen presentation and innate antiviral immunity is higher in the intestine than in any other tissue. By comparing transcriptome profiles, intestine shows the greatest similarity with the gill, head kidney, spleen, epidermis, and olfactory rosette (descending order), which emphasizes the integrity of the peripheral mucosal system and its strong connections with the major lymphoid organs. T cells-specific genes dominate among the genes co-expressed in these tissues. The transcription signature of CD8+ (86 genes, r > 0.9) includes a master gene of immune tolerance foxp3 and other negative regulators. Different segments of the intestine were compared in a separate experiment, in which expression gradients along the intestine were found across several functional groups of genes. The expression of luminal and intracellular (lysosome) proteases is markedly higher in pyloric caeca and distal intestine respectively. Steroid metabolism and cytochromes P450 are highly expressed in pyloric caeca and mid intestine while the distal intestine harbors genes related to vitamin and iron metabolism. The expression of genes for antigen presenting proteins and immunoglobulins shows a gradual increase towards the distal intestine.publishedVersio

Alternative protein sources in the diet modulate microbiota and functionality in the distal intestine of Atlantic salmon ( Salmo salar )

The present study aimed to investigate whether alternative dietary protein sources modulate the microbial communities in the distal intestine (DI) of Atlantic salmon, and whether alterations in microbiota profiles are reflected in modifications in host intestinal function and health status. A 48-day feeding trial was conducted, in which groups of fish received one of five diets: a reference diet in which fishmeal (diet FM) was the only protein source and four experimental diets with commercially relevant compositions containing alternative ingredients as partial replacements of fishmeal, i.e., poultry meal (diet PM), a mix of soybean meal and wheat gluten (diet SBMWG), a mix of soy protein concentrate and poultry meal (diet SPCPM), and guar meal and wheat gluten (diet GMWG). Samples were taken of DI digesta and mucosa for microbial profiling using high-throughput sequencing and from DI whole tissue for immunohistochemistry and expression profiling of marker genes for gut health. Regardless of diet, there were significant differences between the microbial populations in the digesta and the mucosa in the salmon DI. Microbial richness was higher in the digesta than the mucosa. The digesta-associated bacterial communities were more affected by the diet than the mucosa-associated microbiota. Interestingly, both legume-based diets (SBMWG and GMWG) presented high relative abundance of lactic acid bacteria in addition to alteration in the expression of a salmon gene related to cell proliferation (pcna). It was, however, not possible to ascertain the cause-effect relationship between changes in bacterial communities and the host's intestinal responses to the diets. IMPORTANCE The intestine of cultivated Atlantic salmon shows symptoms of compromised function, which are most likely caused by imbalances related to the use of new feed ingredients. Intestinal microbiota profiling may become in the future a valuable endpoint measurement in order to assess fish intestinal health status and effects of diet. The present study aimed to gain information about whether alternative dietary protein sources modulate the microbial communities in the Atlantic salmon intestine and whether alterations in microbiota profiles are reflected in alterations in host intestinal function and health status. We demonstrate here that there are substantial differences between the intestinal digesta and mucosa in the presence and abundance of bacteria. The digesta-associated microbiota showed clear dependence on the diet composition, whereas mucosa-associated microbiota appeared to be less affected by diet composition. Most important, the study identified bacterial groups associated with diet-induced gut dysfunction that may be utilized as microbial markers of gut health status in fish

Convective quenching of stellar pulsations

Context: we study the convection-pulsation coupling that occurs in cold Cepheids close to the red edge of the classical instability strip. In these stars, the surface convective zone is supposed to stabilise the radial oscillations excited by the kappa-mechanism. Aims: we study the influence of the convective motions onto the amplitude and the nonlinear saturation of acoustic modes excited by kappa-mechanism. We are interested in determining the physical conditions needed to lead to a quenching of oscillations by convection. Methods: we compute two-dimensional nonlinear simulations (DNS) of the convection-pulsation coupling, in which the oscillations are sustained by a continuous physical process: the kappa-mechanism. Thanks to both a frequential analysis and a projection of the physical fields onto an acoustic subspace, we study how the convective motions affect the unstable radial oscillations. Results: depending on the initial physical conditions, two main behaviours are obtained: (i) either the unstable fundamental acoustic mode has a large amplitude, carries the bulk of the kinetic energy and shows a nonlinear saturation similar to the purely radiative case; (ii) or the convective motions affect significantly the mode amplitude that remains very weak. In this second case, convection is quenching the acoustic oscillations. We interpret these discrepancies in terms of the difference in density contrast: larger stratification leads to smaller convective plumes that do not affect much the purely radial modes, while large-scale vortices may quench the oscillations.Comment: 15 pages, 17 figures, 3 tables, accepted for publication in A&

Effect of diets containing a purified soybean trypsin inhibitor on growth performance, digestive proteases and intestinal histology in juvenile sea bream (Sparus aurata L.)

Juvenile sea bream were fed on diets containing 0.0, 2.0 or 4.0 g/kg of a soybean trypsin inhibitor (SBTI) for 30 days. The growth performance, total protease activity and intestinal histology were studied after 0,15 and 30 days of dietary treatment. No signi¢cant di¡erences were found in the weight gain, speci¢c growth rate (SGR) and feed conversion rate in fish fed on inhibitor-supplemented diets when compared with those fed on an inhibitor-free diet. Only the SGR at day 15 decreased signi¢cantly with protease inhibitor inclusion, although this effect was not observed at day 30. In relation to proteolytic activity at day 15, the total protease activity in the distal intestine decreased in ¢sh fed on inhibitor-supplemented diets. Zymograms of these extracts showed that the SBTI reduced the intensity of some proteolytic fractions in the distal intestine. A noticeable reduction in the protease activity of the intestinal content in fish fed onthe highest level of soybean inhibitor (4.0 g/kg) was also observed. However, at day 30, the inhibition e¡ect on these active bands was not detected, and the total protease activity was similar to that in ¢sh fed on an inhibitor-free diet. Histological examination revealed no perceptible differences in the intestinal structure between any of the diet groups. In addition, all ¢shweremaintained under experimentation for 10 more days and fed on an inhibitor-free diet to determine whether the possible effects caused by the protease inhibitor could be reverted.The administration of SBTI-supplemented diets did not affect sea bream growth performance or intestine histology after 30 days, and only a decrease in the total alkaline protease activity was found at day 15

A reusable observer pattern implementation using package templates

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