Spatiotemporal distribution and speciation of silver nanoparticles in the healing wound

Funding: This research was supported by funds from the MIUR-FIRB project number RBFR08M6W8. Acknowledgments: ELGA LabWater is acknowledged for providing the PURELAB Option-Q and Ultra Analytic systems, which produced the ultra-pure water used for Ag determinations. Adam Douglas and Dhinesh Asogan are acknowledged for their technical support during LA-ICP-MS analysis at the University of Venice, and the authors gratefully acknowledge Bill Spence and Teledyne Cetac Technologies for the loan of the laser ablation instrumentation. Laura Molin and ISTM-CNR are acknowledged for MALDI-TOF-MS analysis. The synchrotron experiments were performed on beamline ID21 at the European Synchrotron Radiation Facility (ESRF), Grenoble, France (proposal #CH4121).Peer reviewedPostprin

Active silver nanoparticles for wound healing

In this preliminary study, the silver nanoparticle (Ag NP)-based dressing, ActicoatTM Flex 3, has been applied to a 3D fibroblast cell culture in vitro and to a real partial thickness burn patient. The in vitro results show that Ag NPs greatly reduce mitochondrial activity, while cellular staining techniques show that nuclear integrity is maintained, with no signs of cell death. For the first time, transmission electron microscopy (TEM) and inductively coupled plasma mass spectrometry (ICP-MS) analyses were carried out on skin biopsies taken from a single patient during treatment. The results show that Ag NPs are released as aggregates and are localized in the cytoplasm of fibroblasts. No signs of cell death were observed, and the nanoparticles had different distributions within the cells of the upper and lower dermis. Depth profiles of the Ag concentrations were determined along the skin biopsies. In the healed sample, most of the silver remained in the surface layers, whereas in the unhealed sample, the silver penetrated more deeply. The Ag concentrations in the cell cultures were also determined. Clinical observations and experimental data collected here are consistent with previously published articles and support the safety of Ag NP-based dressing in wound treatment

Development of software alignment algorithms and optimization of the luminosity extraction via alignment of the Pˉ\bar {P}ANDA luminosity detector

Für die Vermessung bekannter und möglicher neuer Charmonium Zustände wird das $\bar {P}$ANDA-Experiment bei FAIR gebaut. Die hohe Impulsauflösung des beschleunigten Antiprotonenstrahls ermöglicht Messungen per Energy-Scan-Methode mit beispielloser Genauigkeit. Zur Normierung und Bestimmung der Wechselwirkungsquerschnitte ist die Kenntnis der Zeit-integrierten und absoluten Luminosität erforderlich, was mit dem Luminositätsdetektor erreicht wird. Bereits kleine Fehlausrichtungen seiner Sensoren führen zu großen Abweichungen der Luminosität, weswegen die Istposition präzise bestimmt und korrigiert werden muss. Der Detektor ist modular in drei Braugruppen aufgebaut, deren Positionen voneinander unabhängig bestimmt und korrigiert werden können. Für jede wurde ein Software Alignment Algorithmus entwickelt. Der Einfluss von so korrigierter Fehlausrichtung auf die Genauigkeit der Luminositätsbestimmung wurde untersucht. Die Luminosität lässt sich mit einer Unsicherheit von weniger als 1% bestimmen.To measure key parameters of charmonium resonances, the $\bar {P}$ANDA detector and experiment will be built. Due to the high momentum resolution of the HESR, it is ideally suited for the energy scan method. To normalize measurements and determine absolute cross sections, the time integrated and absolute luminosities are required. They will be measured and monitored with the PANDA Luminosity Detector. Even small deviations of the sensors from their design positions result in a large deterioration of the luminosity. Therefore the actual positions of the sensors must be determined very precisely and their misalignment must be accounted for during hit reconstruction. The LMD is constructed modularly and the misalignment of three separate sub-assemblies can be determined and corrected for independently. Specialized software algorithms were developed for their alignment. The influence of thusly corrected misalignment has been studied and the luminosity can be determined with an uncertainty below 1%

Precision Jet Measurements at HERA and Determination of α\alphas

The status is reviewed of recent high precision measurements of inclusive-jet, dijet and trijet production in deep-inelastic scattering and photoproduction by the HERA experiments H1 and ZEUS. The measurements are in good agreement with perturbative QCD calculations at next-to-leading order and are used for the extraction of the value of the strong coupling at the mass of the Z boson, \alpha_s(M_Z). The methods and results of the QCD analyses are presented and a summary of the values of \alpha_s(M_Z) from recent jet measurements at HERA is given.Comment: Two figures added, additional references give

Hyperon signatures in the PANDA experiment at FAIR

We present a detailed simulation study of the signatures from the sequential decays of the triple-strange pbar p -> Ω+Ω- -> K+ΛbarK- Λ -> K+pbarπ+K-pπ- process in the PANDA central tracking system with focus on hit patterns and precise time measurement. We present a systematic approach for studying physics channels at the detector level and develop input criteria for tracking algorithms and trigger lines. Finally, we study the beam momentum dependence on the reconstruction efficiency for the PANDA detector