Funding: This research was supported by funds from the MIUR-FIRB project number RBFR08M6W8. Acknowledgments: ELGA LabWater is acknowledged for providing the PURELAB Option-Q and Ultra Analytic systems, which produced the ultra-pure water used for Ag determinations. Adam Douglas and Dhinesh Asogan are acknowledged for their technical support during LA-ICP-MS analysis at the University of Venice, and the authors gratefully acknowledge Bill Spence and Teledyne Cetac Technologies for the loan of the laser ablation instrumentation. Laura Molin and ISTM-CNR are acknowledged for MALDI-TOF-MS analysis. The synchrotron experiments were performed on beamline ID21 at the European Synchrotron Radiation Facility (ESRF), Grenoble, France (proposal #CH4121).Peer reviewedPostprin

2nd Broughton

Adhya

Ahlberg

Ansari

Ansell

AshaRani

Barillo

Bianco

Bikle

Bondarenko

Bullen

Carlo Barbante

Chernousova

Chiara Rigo

Coban

Coombs

Dagmar S. Urgast

Desouza

Federico Benetti

George

Graf

Hackett

Hashmi

Hiram Castillo-Michel

Ivan Mičetić

Ivan Munivrana

Iwata

Jörg Feldmann

Kalantari

Klasen

Lansdown

Larese

Loza

Mandal

Marco Roman

Masood

Michopoulou

Moiemen

Monteiro

Mulley

Nherera

Rigo

Roman

Rowan

Samberg

Sasidharan

Sterling

Tenaud

Tra Thanh

Urgast

Vignesh

Vincenzo Vindigni

Vlachou

Vogt

von Zglinicki

Wang

Warren R. L. Cairns

Werner-Linde

Wijnhoven

Wright

Zhang

The Analyst

English

Funding: This research was supported by funds from the MIUR-FIRB project number RBFR08M6W8. Acknowledgments: ELGA LabWater is acknowledged for providing the PURELAB Option-Q and Ultra Analytic systems, which produced the ultra-pure water used for Ag determinations. Adam Douglas and Dhinesh Asogan are acknowledged for their technical support during LA-ICP-MS analysis at the University of Venice, and the authors gratefully acknowledge Bill Spence and Teledyne Cetac Technologies for the loan of the laser ablation instrumentation. Laura Molin and ISTM-CNR are acknowledged for MALDI-TOF-MS analysis. The synchrotron experiments were performed on beamline ID21 at the European Synchrotron Radiation Facility (ESRF), Grenoble, France (proposal #CH4121).Peer reviewe

Roman, Marco

Rigo,  Chiara

Castillo-Michel,  Hiram

Urgast, Dagmar

Feldmann, Jörg

Munivrana, Ivan

Vindigni, Vincenzo

Mičetić, Ivan

Benetti, Federico

Barbante, Carlo

Cairns, Warren R.L.

Aberdeen University Research

Spatiotemporal distribution and speciation of silver nanoparticles in the healing wound

The medical application of nanomaterials is growing fast. Amongst the most widely used, silver nanoparticles are antimicrobial agents whose key application is the care of burns and chronic wounds. Still, their absorption, distribution, metabolism and excretion behaviour in vivo has not yet been systematically investigated. We collected full-profile specimens of skin from four hospital patients with mid-to-deep thickness burns or equivalent skin wounds, treated with dressings containing silver nanoparticles or silver sulfadiazine. Synchrotron radiation μXRF/μXANES and laser ablation-ICP-MS were used to provide the first semi-quantitative/high resolution direct information on the spatiotemporal distribution and speciation of silver in vivo. The metal was rapidly released onto the wound surface, followed by a significant structure-dependent penetration into the damaged tissues. This was accompanied by sequential processes of metallic silver dissolution, chloride complexation, change to metal-thiol protein complexes, and final mobilization into deeper skin layers towards the vascular networks. Complete local clearance of silver was observed after 12 days of treatment in the case of full healing. The results provide a complete insight into the dynamics of silver in real human wounds, and a new basis for the design of innovative silver nanomaterials with optimal antibacterial efficacy and minimized risk for the patient.The medical application of nanomaterials is growing fast. Amongst the most widely used, silver nanoparticles are antimicrobial agents whose key application is the care of burns and chronic wounds. Still, their absorption, distribution, metabolism and excretion behaviour in vivo has not yet been systematically investigated. We collected full-profile specimens of skin from four hospital patients with mid-to-deep thickness burns or equivalent skin wounds, treated with dressings containing silver nanoparticles or silver sulfadiazine. Synchrotron radiation μXRF/μXANES and laser ablation-ICP-MS were used to provide the first semi-quantitative/high resolution direct information on the spatiotemporal distribution and speciation of silver in vivo. The metal was rapidly released onto the wound surface, followed by a significant structure-dependent penetration into the damaged tissues. This was accompanied by sequential processes of metallic silver dissolution, chloride complexation, change to metal-thiol protein complexes, and final mobilization into deeper skin layers towards the vascular networks. Complete local clearance of silver was observed after 12 days of treatment in the case of full healing. The results provide a complete insight into the dynamics of silver in real human wounds, and a new basis for the design of innovative silver nanomaterials with optimal antibacterial efficacy and minimized risk for the patient. This journal i

Rigo, Chiara

Castillo-Michel, Hiram

Urgast, Dagmar S

Feldmann, Jörg

Mičetić, Ivan

Cairns, Warren R L

Archivio istituzionale della ricerca - Università degli Studi di Venezia Ca' Foscari

Crossref

The medical application of nanomaterials is growing fast. Amongst the most widely used, silver nanoparticles are antimicrobial agents whose key application is the care of burns and chronic wounds. Still, their absorption, distribution, metabolism and excretion behaviour in vivo has not yet been systematically investigated. We collected full-profile specimens of skin from four hospital patients with mid-to-deep thickness burns or equivalent skin wounds, treated with dressings containing silver nanoparticles or silver sulfadiazine. Synchrotron radiation μXRF/μXANES and laser ablation-ICP-MS were used to provide the first semi-quantitative/high resolution direct information on the spatiotemporal distribution and speciation of silver in vivo. The metal was rapidly released onto the wound surface, followed by a significant structure-dependent penetration into the damaged tissues. This was accompanied by sequential processes of metallic silver dissolution, chloride complexation, change to metal-thiol protein complexes, and final mobilization into deeper skin layers towards the vascular networks. Complete local clearance of silver was observed after 12 days of treatment in the case of full healing. The results provide a complete insight into the dynamics of silver in real human wounds, and a new basis for the design of innovative silver nanomaterials with optimal antibacterial efficacy and minimized risk for the patient

Archivio istituzionale della ricerca - Università di Padova

https://aura.abdn.ac.uk/bitstream/2164/17005/1/Roman_et_al_Analyst_SpatiotemporalDistributionAnd_AAM.pdf

Spatiotemporal distribution and speciation of silver nanoparticles in the healing wound

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Aberdeen University Research

Archivio istituzionale della ricerca - Università degli Studi di Venezia Ca' Foscari

Crossref

Archivio istituzionale della ricerca - Università di Padova