9 research outputs found
Cardiopulmonary toxicity of peat wildfire particulate matter and the predictive utility of precision cut lung slices
BackgroundEmissions from a large peat fire in North Carolina in 2008 were associated with increased hospital admissions for asthma and the rate of heart failure in the exposed population. Peat fires often produce larger amounts of smoke and last longer than forest fires, however few studies have reported on their toxicity. Moreover, reliable alternatives to traditional animal toxicity testing are needed to reduce the number of animals required for hazard identification and risk assessments.MethodsSize-fractionated particulate matter (PM; ultrafine, fine, and coarse) were obtained from the peat fire while smoldering (ENCF-1) or when nearly extinguished (ENCF-4). Extracted samples were analyzed for chemical constituents and endotoxin content. Female CD-1 mice were exposed via oropharyngeal aspiration to 100μg/mouse, and assessed for relative changes in lung and systemic markers of injury and inflammation. At 24h post-exposure, hearts were removed for ex vivo functional assessments and ischemic challenge. Lastly, 8mm diameter lung slices from CD-1 mice were exposed (11μg) ± co-treatment of PM with polymyxin B (PMB), an endotoxin-binding compound.ResultsOn an equi-mass basis, coarse ENCF-1PM had the highest endotoxin content and elicited the greatest pro-inflammatory responses in the mice including: increases in bronchoalveolar lavage fluid protein, cytokines (IL-6, TNF-α, and MIP-2), neutrophils and intracellular reactive oxygen species (ROS) production. Exposure to fine or ultrafine particles from either period failed to elicit significant lung or systemic effects. In contrast, mice exposed to ENCF-1 ultrafine PM developed significantly decreased cardiac function and greater post-ischemia-associated myocardial infarction. Finally, similar exposures to mouse lung slices induced comparable patterns of cytokine production; and these responses were significantly attenuated by PMB.ConclusionsThe findings suggest that exposure to coarse PM collected during a peat fire causes greater lung inflammation in association with endotoxin and ROS, whereas the ultrafine PM preferentially affected cardiac responses. In addition, lung tissue slices were shown to be a predictive, alternative assay to assess pro-inflammatory effects of PM of differing size and composition. Importantly, these toxicological findings were consistent with the cardiopulmonary health effects noted in epidemiologic reports from exposed populations
Near-Road Modeling and Measurement of Cerium-Containing Particles Generated by Nanoparticle Diesel Fuel Additive Use
Cerium oxide nanoparticles
(nCe) are used as a fuel-borne catalyst
in diesel engines to reduce particulate emissions, yet the environmental
and human health impacts of the exhaust particles are not well understood.
To bridge the gap between emission measurements and ambient impacts,
size-resolved measurements of particle composition and mass concentration
have been performed in Newcastle-upon-Tyne, United Kingdom, where
buses have used an nCe additive since 2005. These observations show
that the noncrustal cerium fraction thought to be associated with
the use of nCe has a mass concentration ∼0.3 ng m<sup>–3</sup> with a size distribution peaking at 100–320 nm in aerodynamic
diameter. Simulations with a near-roadway multicomponent sectional
aerosol dynamic model predict that the use of nCe additives increases
the number concentration of nuclei mode particles (<50 nm in diameter)
while decreasing the total mass concentration. The near-road model
predicts a downwind mass size distribution of cerium-containing particles
peaking at 150 nm in aerodynamic diameter, a value similar to that
measured for noncrustal cerium in Newcastle. This work shows that
both the emission and atmospheric transformation of cerium-containing
particles needs to be taken into account by regional modelers, exposure
scientists, and policymakers when determining potential environmental
and human health impacts