7 research outputs found
Four-part differential leukocyte count using μflow cytometer
- Publication venue
- 'Institute of Electrical and Electronics Engineers (IEEE)'
- Publication date
- 01/01/2010
- Field of study
This paper reports the four-part differential leukocyte
count (DLC) of human blood using a MEMS
microflow (μflow) cytometer. It is achieved with a
two-color laser-induced fluorescence (LIF) detection
scheme. Four types of leukocytes including
neutrophils, eosinophils, lymphocytes and monocytes
are identified in blood samples, which are stained by
fluorescein isothiocyanate (FITC) and propidium
iodide (PI). The DLC results show good correlation
with the count from a commercial hematology
analyzer. The whole system is also implemented into a
portable instrument for space application
Platinum Black Electroplated Impedance Particle Sensor
- Publication venue
- 'Institute of Electrical and Electronics Engineers (IEEE)'
- Publication date
- 01/01/2006
- Field of study
We present a micro electrical impedance particle
sensor. To solve the problem of large electrode electrolyte
interface impedance, we electroplated the electrodes with
platinum black. Devices are fabricated with integrated parylene
technology. An electrical model for the system is proposed and
analytic solutions are obtained. Impedance spectra measurement
of the device filled with various media are in excellent agreement
with model analysis. Signals from individual 10 µm polystyrene
beads passing the sensing electrodes are successfully obtained
Lifestyles advice in primary care
- Publication venue
- Publication date
- 01/01/1994
- Field of study
SIGLEGBUnited Kingdo
Fluorescent labeling, sensing, and differentiation of leukocytes from undiluted whole blood samples
- Publication venue
- 'Elsevier BV'
- Publication date
- 01/01/2007
- Field of study
In this paper, we demonstrated leukocyte labeling, sensing and differentiation from undiluted human whole blood samples with microfabricated
devices.A challenging issue in leukocyte sensing from blood samples is the required high-dilution level, which is used mainly to prevent interference
from the overwhelmingly outnumbered erythrocytes. Dilution is undesirable for micro hemacytometers. It not only increases sample volume and
processing time, but also requires mixing and buffer storage for on-chip implementation. Unlike commercial bulk instruments and previous efforts
by other groups, we completely eliminated the requirement for dilution by staining leukocytes specifically with fluorescent dye acridine orange
(AO) in undiluted human whole blood and then sensing them in microfluidic devices. Green fluorescent signal centered at 525 nm is used for
leukocyte count and red fluorescent signal centered at 650 nm is used for leukocyte differentiation. Throughput of 1000 leukocytes per second was
achieved
Analysis of the Spectroscopic Aspects of Cationic Dye Basic Orange 21
- Author
- Publication venue
- 'American Chemical Society (ACS)'
- Publication date
- Field of study
Analysis of the Spectroscopic Aspects of Cationic Dye Basic Orange 21
- Author
- Publication venue
- Publication date
- Field of study
Spectroscopic properties of cationic
dye basic orange 21 (BO21)
in solutions, in solids, and within leukocytes were examined. Results
obtained with solutions indicate that influence of variables such
as pH, viscosity, salt composition, and various proteins on the absorption
spectrum of BO21 is negligible. However, in the presence of heparin,
a blue shift (484–465 nm) is observed, which is attributed
to the aggregation of BO21 on the polyanion. Applying density functional
theory demonstrates that in aqueous solutions (a) the formation of
BO21 oligomers is thermodynamically favorable, they are oriented in
an antiparallel dipolar arrangement, and their binding energies are
lower than those of parallel dipolar arrangements, (b) association
between BO21 aggregates and heparin is highly favorable, and (c) the
blue shift is due to the mixing of π → π* transitions
caused by BO21 molecule stacking. However, when embedded in basophils,
the absorption spectra of intracellular BO21 is extremely red-shifted,
with two peaks (at 505 and 550 nm) found to be attributed to BO21
and the BO21–heparin complex, respectively, which are intracellularly
hosted in nonaqueous environments. Initial evidence of the ability
to differentiate between leukocyte types by BO21 is presented
Intoxikationen
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- RL Wechsler
- RM Paddison
- RM Wilder
- RM Wilder
- Rodenacker
- Roncati
- Roncati
- Rosenblath
- Rossle
- RP Bergner
- RP Custer
- RP Smith
- RP Strong
- RR Grimier
- RR Grimier
- RR Sealock
- RS Banay
- RS Diaz-Rivera
- RT Clark Jr.
- RT Hewitt
- RT Johnstone
- RW Brain
- RW Gerard
- RW Gerard
- RW Thorp
- Röthig
- S Arieti
- S Benjamin
- S Bojlnov
- S Botbelo
- S Brock
- S Cobb
- S Cobb
- S Cole
- S Erlandsson
- S Genkin
- S Goldplam
- S Gordy
- S Gordy
- S Gray
- S Grzycxi
- S Handovsky
- S Hirschhorn
- S Hoffmann
- S Kahoun
- S Katzenelenbogen
- S Katzenelenbogen
- S Kubota
- S Környey
- S Környey
- S Környey
- S Környey
- S Környey
- S Környey
- S Levy
- S Litzner
- S Moeschlin
- S Moescrlin
- S Moesohlin
- S Moschini
- S Nesbitt
- S Nesbitt
- S Pap
- S Petersen
- S Pisani
- S Ruff
- S Ruff
- S Saltykow
- S Salén
- S Scheidegger
- S Scheidegger
- S Scheideoger
- S Sosxne
- S Spiegelman
- S Swaroop
- S Tolone
- S Tolone
- S Wawzonek
- S Wilson
- S Winblad
- SA Mackeith
- SA Mccounuck
- Sacerdotti
- SAK Wilson
- SAK Wilsox
- SAK Wilsox
- Salmon
- Samejuma
- Sattler
- SB Schryver
- SB Wolbach
- SB Wolbach
- SB Wortis
- SB Wortis
- SC Kasdan
- SC Swift
- Schelcher
- Schlokow
- Schnitzler
- Schnyder
- Schoute
- Schuster
- Schwalbe
- Schweinitz
- SD Ingham
- SE Gould
- SE Monroe
- Seelert
- Seelert
- Seifried
- Selbach
- Semidaloff
- SG Ross
- Shimazono
- SJ Lerro
- SL Last
- SL Wilens
- Smetana
- Smicnovicz
- SN Sarkar
- Sodré
- SP Hicks
- SP Hicks
- SP Hicks
- SP Hicks
- SP Hicks
- SP Hicks
- Spiethoff
- Spillmann
- SRH Shah
- SS Blackman Jr.
- SS Blackman Jr.
- SS Buckley
- SS Ketty
- SS Korsakow
- SS Korsakow
- SS Siengalewicz
- Steinbrinck
- Stewart
- Stiefler
- Stine
- Strong
- Stubenrauch
- Stuelp
- Stursrerg
- Störring
- Suzuki
- SW Gillman
- SW Hoobler
- SW Lmpincott
- Szczepanski
- Sézary
- T Afendulis
- T Alajouanine
- T Alajouanine
- T Alajouanine
- T Arajouanine
- T Brander
- T Broman
- T Browan
- T Ericsson
- T Kang
- T Leary
- T Lehoczky de
- T Lewis
- T Lids
- T Linz
- T Mclardy
- T Ohkuma
- T Röder-Kutsch
- T Schreuss
- T Schroeder
- T Schöndorf
- T Seldon
- T Simon
- T Sollmann
- T Spiegel
- T Sutton
- T Suzucki
- T Tanaka
- T Upners
- T Upners
- TA Gonzales
- Takasu
- Takeya-Sikô
- Takeya-Sikô
- TAL Davies
- Tarpeiner
- Tauber
- TC Butler
- TC Godwin
- TCM Young
- TD Rivers
- TD Spies
- TE Bachhuber
- TE Machella de
- TF Dixon
- TG Stewart
- TIC Storch
- TJ Fatherree
- TJ Kennedy Jr.
- TJ Putnam
- TL Althausen
- TL Duffy
- Traina
- Trimborn
- Trossarelli
- Tröger
- TS Evans
- U Bassi
- U Bassi
- U Cerletti
- U Friedeman
- U Friedeman
- U Luft
- U Mrrsutasii
- U Testa
- U Vollrate
- V Adinolfi
- V Albertini
- V Ascoli
- V Becker
- V Benoit
- V Calvo
- V Dhers
- V Fellenius
- V Gaupp
- V Kesztele
- V Koszler
- V Lachnpt
- V Leigheb
- V Meduna
- V Menon
- V Moellendorff
- V Monakow
- V Plavec
- V Tschisch
- V Zahle
- Vas
- Velden van der
- VH Vogel
- Villaverde
- Villaverde
- Villaverde
- Villaverde
- Villaverde
- VR Mason
- VR Mason
- W Altmann
- W Anschitz
- W Arcisz
- W Arnold
- W Beiglböck
- W Berger
- W Block
- W Blume
- W Blume
- W Blume
- W Boor de
- W Brainrussel
- W Bredemann
- W Brown
- W Cone
- W Cordes
- W Dameshek
- W Dontenwill
- W Ehrhardt
- W Filehne
- W Friemann
- W Gennerich
- W Goldfarb
- W Guiljarowski
- W Hay
- W Haymaker
- W Heine
- W Heineke
- W Heineke
- W Heinichen
- W Herkel
- W Hesse
- W Heubner
- W Heubner
- W Hoerburger
- W Hortsch
- W Huecx
- W Höfer
- W Höirer
- W Kat
- W Kirschbaum
- W Klein
- W Kürbitz
- W Laubender
- W Laubmann
- W Ludwig
- W Mahoney
- W Noell
- W Noell
- W Noell
- W Noell
- W Noell
- W Palmer
- W Paulus
- W Reiml
- W Reisert
- W Riese
- W Riese
- W Roehl
- W Rotter
- W Rotter
- W Rotter
- W Rotter
- W Schclz
- W Scholz
- W Scholz
- W Scholz
- W Scholz
- W Scholz
- W Scholz
- W Scholz
- W Scholz
- W Scholz
- W Schopper
- W Schorz
- W Schulemann
- W Schwarzacher
- W Schütze
- W Souullenberg
- W Spalteholz
- W Spielmeyer
- W Spielmeyer
- W Spielmeyer
- W Spielmeyer
- W Spielmeyer
- W Spielmeyer
- W Stecher
- W Steinbrinck
- W Stepp
- W Stich
- W Stocker
- W Straub
- W Uhthoff
- W Uhthoff
- W Uhthoff
- W Uhthoff
- W Wechselmann
- W Wechselmann
- W Weigeldt
- W Weigeldt
- W Weimann
- W Weimann
- W Weimann
- W Weimann
- W Weimann
- W Weimann
- W Weimann
- W Weimann
- W Weise
- W Wild
- WA Bastedo
- WA Horwitz
- WA Jacobs
- WA Jacobs
- WA Jacobs
- Wachholz
- Wagner-Jauregg
- Wassmuth
- WB Cadwalader
- WB Chew
- WC Black
- WD Mcnally
- WD Mcnally
- WD Vögtlin
- WE Clark
- WE Dixon
- WE Loomis
- WE Machle
- Weese
- Westphal
- WF Oettingen
- WG Haynes
- WG Lennox
- WG Lennox
- WG Lennox
- WG Lennox
- WG Spuiller
- WH Büel
- WH Eddy
- WH Hill
- WH Sebrell
- WH Sebrell
- Wieil
- Wiese
- Wirth
- WJ Buchanan
- WJ Dilling
- WJ Eicke
- WJ Eicre
- WJ Horsey
- WJ Horsey
- WJ Mccardie
- WJ Turner
- WJ Waters
- WJ Waters
- WJC Verhaart
- WJC Verhaart
- WJC Verhaart
- WK Hare
- WL Craddock
- WL Lillie
- WL Ritter
- WL Vögtlin
- WL Vögtlin
- WM Stephens
- WM Yater
- WN Aldrige
- WN Bispham
- WN Kemp
- WO Umiker
- Wohlwill
- Wolkow
- WP Lawson
- WR Bowen
- WR Hess
- WR Hess
- WR Hess
- WR Murray
- WT Haverfield
- WT Oettingen
- WU Mcclenahan
- WW Jetter
- WW Jetter
- WW Woons
- Y Jacksch
- Y Okamoto
- Y Tsiminakis
- YC Chang
- Z Swetnik
- Z Wechsler
- Z Wechsler
- Zangger
- Ziegler
- Zutt
- Ä Swansson
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- 01/01/1958
- Field of study