Region-wide glacier mass balances over the Pamir-Karakoram-Himalaya during 1999-2011 (vol 7, pg 1263, 2013)

ISI Document Delivery No.: 273OY Times Cited: 0 Cited Reference Count: 1 Cited References: Gardelle J, 2013, CRYOSPHERE, V7, P1263, DOI 10.5194/tc-7-1263-2013 Gardelle, J. Berthier, E. Arnaud, Y. Kaab, A. 0 COPERNICUS GESELLSCHAFT MBH GOTTINGEN CRYOSPHEREThe recent evolution of Pamir-Karakoram- Himalaya (PKH) glaciers, widely acknowledged as valuable high-altitude as well as mid-latitude climatic indicators, remains poorly known. To estimate the regionwide glacier mass balance for 9 study sites spread from the Pamir to the Hengduan Shan (eastern Himalaya), we compared the 2000 Shuttle Radar Topography Mission (SRTM) digital elevation model (DEM) to recent (2008- 2011) DEMs derived from SPOT5 stereo imagery. During the last decade, the region-wide glacier mass balances were contrasted with moderate mass losses in the eastern and central Himalaya (−0.22±0.12mw.e. yr−1 to −0.33±0.14mw.e. yr−1) and larger losses in the western Himalaya (−0.45±0.13mw.e. yr−1). Recently reported slight mass gain or balanced mass budget of glaciers in the central Karakoram is confirmed for a larger area (+0.10±0.16mw.e. yr−1) and also observed for glaciers in the western Pamir (+0.14±0.13mw.e. yr−1). Thus, the "Karakoram anomaly" should be renamed the "Pamir- Karakoram anomaly", at least for the last decade. The overall mass balance of PKH glaciers, −0.14±0.08mw.e. yr−1, is two to three times less negative than the global average for glaciers distinct from the Greenland and Antarctic ice sheets. Together with recent studies using ICESat and GRACE data, DEM differencing confirms a contrasted pattern of glacier mass change in the PKH during the first decade of the 21st century

Persistent Scatterer Interferometry (PSI) technique for landslide characterization and monitoring

: The measurement of landslide superficial displacement often represents the most effective method for defining its behavior, allowing one to observe the relationship with triggering factors and to assess the effectiveness of the mitigation measures. Persistent Scatterer Interferometry (PSI) represents a powerful tool to measure landslide displacement, as it offers a synoptic view that can be repeated at different time intervals and at various scales. In many cases, PSI data are integrated with in situ monitoring instrumentation, since the joint use of satellite and ground-based data facilitates the geological interpretation of a landslide and allows a better understanding of landslide geometry and kinematics. In this work, PSI interferometry and conventional ground-based monitoring techniques have been used to characterize and to monitor the Santo Stefano d’Aveto landslide located in the Northern Apennines, Italy. This landslide can be defined as an earth rotational slide. PSI analysis has contributed to a more in-depth investigation of the phenomenon. In particular, PSI measurements have allowed better redefining of the boundaries of the landslide and the state of activity, while the time series analysis has permitted better understanding of the deformation pattern and its relation with the causes of the landslide itself. The integration of ground-based monitoring data and PSI data have provided sound results for landslide characterization. The punctual information deriving from inclinometers can help in defining the actual location of the sliding surface and the involved volumes, while the measuring of pore water pressure conditions or water table level can suggest a correlation between the deformation patterns and the triggering factors

Rate-dependency of action potential duration and refractoriness in isolated myocytes from the rabbit AV node and atrium

During atrial fibrillation, ventricular rate is determined by atrioventricular nodal (AVN) conduction, which in part is dependent upon the refractoriness of single AVN cells. The aims of this study were to investigate the rate-dependency of the action potential duration (APD) and effective refractory period (ERP) in single myocytes isolated from the AV node and atrium of rabbit hearts, using whole cell patch clamping, and to determine the contribution of the 4-aminopyridine (4-AP)-sensitive current, ITO1to these relationships in the two cell types. AVN cells had a more positive maximum diastolic potential (-60±1 v-71±2 mV), lower Vmax(8±2 v 144±17 V/s) and higher input resistance [420±46 v 65±7 MOHgr (mean±s.eP<0.05n=9–33)], respectively, than atrial myocytes. Stepwise increases in rate from 75 beats/min caused activation failure and Wenckebach periodicity in AVN cells (at around 400 beats/min), but 1:1 activation in atrial cells (at up to 600 beats/min). Rate reduction from 300 to 75 beats/min shortened the ERP in both cell types (from 155±7 to 135±11 ms in AVN cells [P<0.05, n=6] and from 130±8 to 106±7 ms in atrial cells [P<0.05, n=10]). Rate increase from 300 to 480 and 600 beats/min shortened ERP in atrial cells, by 12±4% (n=8) and 26±7% (n=7), respectively (P<0.05). By contrast, AVN ERP did not shorten at rates >300 beats/min. In atrial cells, rate reduction to 75 beats/min caused marked shortening of APD50(from 51±6 to 29±6 ms, P<0.05). 4-AP (1 mm) significantly prolonged atrial APD50at 75 beats/min (P<0.05, n=7), but not at 300 or 400 beats/min. In AVN cells, in contrast, there was less effect of rate change on APD, and 4-AP did not alter APD50at any rate. 4-AP also did not affect APD90or ERP in either cell type. In conclusion, a lack of ERP-shortening at high rates in rabbit single AVN cells may contribute to ventricular rate control. ITO1contributed to the APD50rate relation in atrial, but not AVN cells and did not contribute to the ERP rate relation in either cell type

Functional Characterization of Rare Variants in the SHOX2 Gene Identified in Sinus Node Dysfunction and Atrial Fibrillation

Sinus node dysfunction (SND) and atrial fibrillation (AF) often coexist; however, the molecular mechanisms linking both conditions remain elusive. Mutations in the homeobox-containing SHOX2 gene have been recently associated with early-onset and familial AF. Shox2 is a key regulator of sinus node development, and its deficiency leads to bradycardia, as demonstrated in animal models. To provide an extended SHOX2 gene analysis in patients with distinct arrhythmias, we investigated SHOX2 as a susceptibility gene for SND and AF by screening 98 SND patients and 450 individuals with AF. The functional relevance of the novel mutations was investigated in vivo and in vitro, together with the previously reported p.H283Q variant. A heterozygous missense mutation (p.P33R) was identified in the SND cohort and four heterozygous variants (p.G77D, p.L129=, p.L130F, p.A293=) in the AF cohort. Overexpression of the pathogenic predicted mutations in zebrafish revealed pericardial edema for p.G77D and the positive control p.H283Q, whereas the p.P33R and p.A293= variants showed no effect. In addition, a dominant-negative effect with reduced heart rates was detected for p.G77D and p.H283Q. In vitro reporter assays demonstrated for both missense variants p.P33R and p.G77D significantly impaired transactivation activity, similar to the described p.H283Q variant. Also, a reduced Bmp4 target gene expression was revealed in zebrafish hearts upon overexpression of the p.P33R mutant. This study associates additional rare variants in the SHOX2 gene implicated in the susceptibility to distinct arrhythmias and allows frequency estimations in the AF cohort (3/990). We also demonstrate for the first time a genetic link between SND and AF involving SHOX2. Moreover, our data highlight the importance of functional investigations of rare variants

Error sources and guidelines for quality assessment of glacier area, elevation change, and velocity products derived from satellite data in the Glaciers_cci project

Satellite data provide a large range of information on glacier dynamics and changes. Results are often reported, provided and used without consideration of measurement accuracy (difference to a true value) and precision (variability of independent assessments). Whereas accuracy might be difficult to determine due to the limited availability of appropriate reference data and the complimentary nature of satellite measurements, precision can be obtained from a large range of measures with a variable effort for determination. This study provides a systematic overview on the factors influencing accuracy and precision of glacier area, elevation change (from altimetry and DEM differencing), and velocity products derived from satellite data, along with measures for calculating them. A tiered list of recommendations is provided (sorted for effort from Level 0 to 3) as a guide for analysts to apply what is possible given the datasets used and available to them. The more simple measures to describe product quality (Levels 0 and 1) can often easily be applied and should thus always be reported. Medium efforts (Level 2) require additional work but provide a more realistic assessment of product precision. Real accuracy assessment (Level 3) requires independent and coincidently acquired reference data with high accuracy. However, these are rarely available and their transformation into an unbiased source of information is challenging. This overview is based on the experiences and lessons learned in the ESA project Glaciers_cci rather than a review of the literature

An angiopoietin 2, FGF23, and BMP10 biomarker signature differentiates atrial fibrillation from other concomitant cardiovascular conditions

Early detection of atrial fibrillation (AF) enables initiation of anticoagulation and early rhythm control therapy to reduce stroke, cardiovascular death, and heart failure. In a cross-sectional, observational study, we aimed to identify a combination of circulating biomolecules reflecting different biological processes to detect prevalent AF in patients with cardiovascular conditions presenting to hospital. Twelve biomarkers identified by reviewing literature and patents were quantified on a high-precision, high-throughput platform in 1485 consecutive patients with cardiovascular conditions (median age 69 years [Q1, Q3 60, 78]; 60% male). Patients had either known AF (45%) or AF ruled out by 7-day ECG-monitoring. Logistic regression with backward elimination and a neural network approach considering 7 key clinical characteristics and 12 biomarker concentrations were applied to a randomly sampled discovery cohort (n=933) and validated in the remaining patients (n=552). In addition to age, sex, and body mass index (BMI), BMP10, ANGPT2, and FGF23 identified patients with prevalent AF (AUC 0.743 [95% CI 0.712, 0.775]). These circulating biomolecules represent distinct pathways associated with atrial cardiomyopathy and AF. Neural networks identified the same variables as the regression-based approach. The validation using regression yielded an AUC of 0.719 (95% CI 0.677, 0.762), corroborated using deep neural networks (AUC 0.784 [95% CI 0.745, 0.822]). Age, sex, BMI and three circulating biomolecules (BMP10, ANGPT2, FGF23) are associated with prevalent AF in unselected patients presenting to hospital. Findings should be externally validated. Results suggest that age and different disease processes approximated by these three biomolecules contribute to AF in patients. Our findings have the potential to improve screening programs for AF after external validation

Zn treatment effects on biological potential of fennel bulbs as affected by in vitro digestion process

Zn treatment effects on the stability of polyphenols, MDA (malondialdehyde) content, antioxidant and lipoxygenase inhibition activities of two varieties of fennel bulbs were studied by using an in vitro gastrointestinal digestion model. Likewise, the effect of Zn on viability cells of E. coli was also performed. The results revealed that high amounts of total phenolic and flavonoid compounds were released during the digestion process, especially after the intestinal phase. Additionally, the antioxidant and lipoxygenase inhibitory activity were affected by the gastrointestinal digestion process and seems to be correlated with total phenol contents. On the other hand, the viability of E. coli was not affected by the activity of our tested bulbs during passage through the artificial digestion model, but the treated bulbs activity contribute relatively to the inhibition growth of bacteria. The survival of E. coli in fennel bulbs was challenged with simulated gastrointestinal fluids and the results showed that the E. coli strains, despite having experienced a viability reduction at the intestinal phase, were able to overcome the exposure to the gastrointestinal synthetic fluids. This E. coli ability reinforces the need for good hygienic measures to assure safe fresh produce, even for those that are rich in antibacterial compounds.info:eu-repo/semantics/publishedVersio

Small Oscillatory Accelerations, Independent of Matrix Deformations, Increase Osteoblast Activity and Enhance Bone Morphology

A range of tissues have the capacity to adapt to mechanical challenges, an attribute presumed to be regulated through deformation of the cell and/or surrounding matrix. In contrast, it is shown here that extremely small oscillatory accelerations, applied as unconstrained motion and inducing negligible deformation, serve as an anabolic stimulus to osteoblasts in vivo. Habitual background loading was removed from the tibiae of 18 female adult mice by hindlimb-unloading. For 20 min/d, 5 d/wk, the left tibia of each mouse was subjected to oscillatory 0.6 g accelerations at 45 Hz while the right tibia served as control. Sham-loaded (n = 9) and normal age-matched control (n = 18) mice provided additional comparisons. Oscillatory accelerations, applied in the absence of weight bearing, resulted in 70% greater bone formation rates in the trabeculae of the metaphysis, but similar levels of bone resorption, when compared to contralateral controls. Quantity and quality of trabecular bone also improved as a result of the acceleration stimulus, as evidenced by a significantly greater bone volume fraction (17%) and connectivity density (33%), and significantly smaller trabecular spacing (−6%) and structural model index (−11%). These in vivo data indicate that mechanosensory elements of resident bone cell populations can perceive and respond to acceleratory signals, and point to an efficient means of introducing intense physical signals into a biologic system without putting the matrix at risk of overloading. In retrospect, acceleration, as opposed to direct mechanical distortion, represents a more generic and safe, and perhaps more fundamental means of transducing physical challenges to the cells and tissues of an organism

Variable Nav1.5 Protein Expression from the Wild-Type Allele Correlates with the Penetrance of Cardiac Conduction Disease in the Scn5a+/− Mouse Model

BACKGROUND: Loss-of-function mutations in SCN5A, the gene encoding Na(v)1.5 Na+ channel, are associated with inherited cardiac conduction defects and Brugada syndrome, which both exhibit variable phenotypic penetrance of conduction defects. We investigated the mechanisms of this heterogeneity in a mouse model with heterozygous targeted disruption of Scn5a (Scn5a(+/-) mice) and compared our results to those obtained in patients with loss-of-function mutations in SCN5A. METHODOLOGY/PRINCIPAL FINDINGS: Based on ECG, 10-week-old Scn5a(+/-) mice were divided into 2 subgroups, one displaying severe ventricular conduction defects (QRS interval>18 ms) and one a mild phenotype (QRS53 weeks), ajmaline effect was larger in the severely affected subgroup. These data matched the clinical observations on patients with SCN5A loss-of-function mutations with either severe or mild conduction defects. Ventricular tachycardia developed in 5/10 old severely affected Scn5a(+/-) mice but not in mildly affected ones. Correspondingly, symptomatic SCN5A-mutated Brugada patients had more severe conduction defects than asymptomatic patients. Old severely affected Scn5a(+/-) mice but not mildly affected ones showed extensive cardiac fibrosis. Mildly affected Scn5a(+/-) mice had similar Na(v)1.5 mRNA but higher Na(v)1.5 protein expression, and moderately larger I(Na) current than severely affected Scn5a(+/-) mice. As a consequence, action potential upstroke velocity was more decreased in severely affected Scn5a(+/-) mice than in mildly affected ones. CONCLUSIONS: Scn5a(+/-) mice show similar phenotypic heterogeneity as SCN5A-mutated patients. In Scn5a(+/-) mice, phenotype severity correlates with wild-type Na(v)1.5 protein expression