Cytocompatible In Situ Cross-Linking of Degradable LbL Films Based on Thiol-Exchange Reaction

Formation of both mechanically durable and programmably degradable layer-by-layer (LbL) films in a biocompatible fashion has potential applications in cell therapy, tissue engineering, and drug-delivery systems, where the films are interfaced with living cells. In this work, we developed a simple but versatile method for generating in situ cross-linked and responsively degradable LbL films, based on the thiol-exchange reaction, under highly cytocompatible conditions (aqueous solution at pH 7.4 and room temperature). The cytocompatibility of the processes was confirmed by coating individual yeast cells with the cross-linked LbL films and breaking the films on demand, while maintaining the cell viability. In addition, the processes were applied to the controlled release of an anticancer drug in the HeLa cells.close1

Nanoplasmonic smooth silica versus porous calcium carbonate bead biosensors for detection of biomarkers

Nanoplasmonic biosensors based on gold nanoparticle functionalized smooth silica and porous calcium carbonate particles are presented. It is identified in this comparative study the role of porosity for adsorbing gold nanoparticles and subsequent detection of biomarkers. That is further applied in this study for detection of biomarkers. Detection of glucose a biomarker of diabetes is studied together with that of bovine serum albumin a very relevant bio-molecule. Raman scattering is used for label-free detection of molecules in the sub-mu MmM range detection capabilities, which covers the range corresponding to healthy and diseased persons. Implications of current study for detection and identification of biomarkers are discussed