Evaluation of total phenols and antioxidant activity of Mullein (Verbascum songaricum) ecotypes

زمینه و هدف: جنس گل ماهور بزرگترین جنس خانواده گل میمون است. اندام های این گیاه حاوی مواد موثره مانند فنول ها و موسیلاژ هستند. هدف از این تحقیق بررسی میزان ترکیبات فنلی تام و فعالیت آنتی اکسیدانی گل ماهور در جنوب غرب ایران بود. روش بررسی: در این مطالعه 10 اکوتیپ از گونه Verbascum songaricum ارزیابی شدند. استخراج و اندازه‌گیری فنول کل به روش فولین سیوکالتیو انجام شد. فعالیت آنتی‌اکسیدانی عصاره های نمونه های برگ، با استفاده از روش DPPH اندازه‌گیری شد. یافته‌ها: نتایج بدست آمده نشان داد که محتوای فنلی و فعالیت آنتی‌اکسیدانی اکوتیپ‌های مختلف متفاوت بود. بیشترین و کمترین میزان فنول کل عصاره برگ به ترتیب از اکوتیپ منطقه اردل (mg GAE/g DW 92/192) و منطقه میمند (mg GAE/g DW 71/107) بدست آمد. بیشترین فعالیت آنتی‌اکسیدانی مربوط به اکوتیپ منطقه اردل (g/mlμ 09/31 IC50: ) بود که با سایر اکوتیپ‌های گل ماهور تفاوت معنی‌داری داشت. نتیجه گیری: میزان فنول کل و فعالیت آنتی اکسیدانی نمونه ها متاثر از شرایط اقلیمی محل رویش بود. با توجه به میزان بالایی فنول و فعالیت آنتی‌اکسیدانی به نظر می­رسد گیاه دارویی گل ماهور گزینه مناسبی به عنوان یک آنتی­اکسیدان در درمان ­های مکمل باشد

Efficacy of Various Insecticides on Safflower fly, Acanthiophilus helianthi Rossi (Diptera: Tephritidae) in Kohgiloyeh and Boyerahmad Province (Iran).

Safflower fly (Acanthiophilus helianthi Rossi) is one of the main limiting factors to expand the production area of the crop in several countries. Since use of selective insecticides is one of the most important methods for pest management, we evaluated the efficacy of six insecticides against A. helianthi infesting safflower. Field studies were conducted at the farm of Agriculture Research Station Gachsaran, in 2008-2009, to determine the effects of six different insecticides on the damage and incidence of A. helianthi on safflower variety ‘Sina’. After first spray overall mean population larvae of A. helianthi inside flower heads was 3.83, 4.95, 5.79, 6.86, 7.67, 8.31 per 10 flower head on Endosulfan 35EC, Chlorpyriphos 20 EC, Monochrotophos 28 EC, Deltamethrin 2.8 EC, Malathion 56 EC, and Supracide 40 EC treated plants respectively, while on control plants the population larvae was 12.59. Similarly, after the second spray of each of the same insecticides the population larvae of A. helianthi was 4.69, 6.25, 7.63, 8.43, 9.37 and 10.75 respectively. Where in controls there were 17.20 larvae of safflower capsule fly. Percent decrease of population of larvae safflower capsule fly in comparison to control after the first spray was highest in Endosulfan 35 EC (74.22) followed by Chlorpyriphos 20 EC (64.93), Monochrotophos 28 EC (56.09), Deltamethrin 2.8 EC (49.63),Malathion 56 EC (40.96) and Supracide 40 EC (37.76). After the second spray percent decrease over control recorded was highest in Endosulfan 35 EC (85.56), followed by Chlorpyriphos 20 EC (73.10), Monochrotophos 28 EC (65.96), Deltamethrin 2.8 EC (62.81), Malathion 56 EC (54.54) and Supracide 40 EC (47.64)

Biodiversity of insects associated with safflower (Carthamus tinctorius) crop in Gachsaran, Iran

The present research conducted to see the biodiversity of insects in warm and cool areas from March to April in 2009 at the Gachsaran Agricultural Research Station. A total number of 4261 specimens, which were identified into 31 families and 92 species. Out of total 31 families collected, 10 families were pests, 2 families belongs to predators and beneficial insects and 7 families belongs to parasitoids and beneficial insects and finally, 12 families of insects are saprophage and polyphagous. There was no significant difference (P>0.05) in the number of insects collected between months. The mean number of insects collected in the month of March, April and May was 34.7, 30.7 and 36.2 respectively. The Basht area is most susceptible host for insects (37.9), while the Gachsaran and Lishter areas is less susceptible (33.5) and (28.4) so both sites not differ significantly in the population of insects. The family Coccinellidae had more population in the Gachsaran-safflower zone (98.3) than the Basht-safflower zone (54.6) and it was significantly similar to the family cicadellidae. Similarly, different families like Aphididae (98.0), Cicadellidae (74), Thripidae (50.0), Curculionidae (26.6), Noctuidae (25.0) and Torymidae (27.3) had higher population in the Gachsaran-safflower zone than in the Basht-safflower area with means of 92.3, 63.0, 42.6, 21.0, 21.6 and 19.3 respectively. Also the families like Nymphalide, Pieridae, Lygaeidae, Papilionidae, Miridae, Pentatomidae, Reduviidae, Geometridae, Gryllotalpidae, Mantidae, Acrididae, Saturnidae and Anthocoridae had mean population of 12.6, 7.4, 12.0, 4.1, 4.4, 8.6, 1.9, 0.5, 7.5, 2.4, 11.3, 3.3 and 8.9

The comparision of essential oil composition from Thymus vulgaris L., Melissa officinalis L. and Achillea millefolium L. in Shahrekord climate condition

In this research, the essential oil composition of Thymus vulgaris L., Melissa officinalis L. and Achillea millefolium L. were evaluated in Shahrekord climate condition. The aerial parts of these plants in full flowering stages were collected in summer 2017 from a research farm of Shahrekord University. The essential oils were extracted by hydro distillation method (clevenger apparatus) and were analyzed by GC and GC/MS. Results were showed that the thymol (33.2%), α-terpinene (18.50%), p-cymene (10.9%) and linalool (5%) were the most components of Thymus vulgaris. Neral (26.3%), geranial (25%), β-caryophyllene (6.9%), geranyl acetate (6.4%) and cis-crysantenol (5.8%) were the most important compound of M. officinalis essential oil and the cis-chrysanthenyl acetate (27.19%), cis-chrysantheol (13.16%), germacren D (7.24%), β-caryophyllene (6.69%) and thymol (6.15%) recorded in Achillea essential oil In conclusion, the results were showed that essential oils of thyme, lemon balm, and yarrow had suitable quality composition in Shahrekord climate conditions

The effect of LINC01296 expression in patients with cancer: A systematic review and meta-analysis

Background: Recently has been suggested that LINC01296 has an important role in tumor-promoting in different malignancies. We performed first meta-analysis to assess the association between the LINC01296 expression and clinicopathological criteria and the survival of patients with cancers. Methods: Relevant articles Identified by PubMed, EMBASE, Web of Science, and Scopus searching between December 2000 and 28 December 2018. Binomial data were evaluated by the odds ratio (OR) as the rapid statistic. The association between overall survival (OS) and the LINC01296 expression was evaluated using pooling the hazard ratio (HR) with its corresponding 95 confidence interval (CI). Results: Finally, 9 studies with 720 patients with cancer were included. The expression of LINC01296 showed a significant positive association with TNM stage (OR = 2.67, 95 CI = 1.83-3.88), tumor stage (OR= 2.22, 95 CI= 1.34-3.66) and lymph node metastasis (OR = 3.07, 95 CI = 2.23-4.21). A shorter OS was significantly associated with the expression of LINC01296 (HR = 3.95, 95 CI = 2.65-5.25) and lymph node metastasis (HR = 2.39, 95 CI =1.16-3.63). The OS did not show significant association with gender (HR = 0.83, 95 CI =-0.63-2.30) and tumor stage (HR= 2.66, 95 CI=-0.22-5.54). Conclusion: In conclusion, the results of this meta-analysis suggest that the expression of LINC01296 might be considered as a potential biomarker in patients with cancer. © 2020 Asian Pacific Organization for Cancer Prevention

Resistance of 14 accessions/cultivars of Lycopersicon spp. to two-spotted spider mite, Tetranychus urticae (Acari: Tetranychidae), in laboratory and greenhouse

Fourteen accessions and cultivars of Lycopersicon spp. were studied to evaluate their resistance to two-spotted spider mite, Tetranychus urticae Koch, under laboratory and greenhouse conditions. In vitro studies were carried out using thumbtack and leaf disk bioassays. Lycopersicon hirsutum and L. pennellii accessions supported more mites on the tack. The highest number of eggs (5.15 ± 0.48 eggs / â / d) was recorded on L. pimpinellifolium LA2533 and the lowest number (0 egg / â / d) was recorded on L. hirsutum and L. pennellii accessions. The highest mite mortality and lowest damage score occurred on the leaf disks of L. pennellii and L. hirsutum accessions that were strikingly in contrast to our observations on L. esculentum varieties (Nandi and Sankranthi). The developmental time of the mite was longest (8.61 ± 0.18 days) on leaf disks of L. esculentum NDTVR-73 and shortest (7.18 ± 0.51 days) on L. pimpinellifolium LA2533. In the greenhouse, the mite was unable to establish on L. hirsutum and L. pennellii accessions. Amongst the rest, Nandi and Sankranthi accessions/cultivars supported the highest, while NDTVR-73 supported the lowest mite population. The highest and lowest density of type VI glandular trichomes were recorded on L. esculentum NDTVR-73 (67.33 ± 6.34 trichomes/mm²) and L. pennellii LA2963 (0.79 ± 0.15 trichomes/mm²), respectively. No relation was found between density of type VI trichomes and resistance to the mite. Type IV glandular trichomes were observed only on the foliages of L. hirsutum and L. pennellii. A positive relation exists between the density of type IV glandular trichomes and resistance to the mite

Effect of Intensity on Changes in Cardiac Autonomic Control of Heart Rate and Arterial Stiffness After Equated Continuous Running Training Programs

[EN] Background: It is well known that exercise training has positive effects on both cardiac autonomic function and arterial stiffness (AS). However, it is not clear that which exercise training variables, intensity or volume, or both, play a crucial role in this regard. This study investigates the chronic effects of high-volume moderate-intensity training (HVMIT) and low-volume high-intensity training (LVHIT) on heart rate variability (HRV) and AS in sedentary adult men. Materials and Methods: Notably, 45 males (age: 42 ± 5.7 years) were randomly assigned to a control (n = 15), HVMIT (n = 15), or LVHIT (n = 15). The HVMIT group ran three times per week on a treadmill at 50–60% of VO2max for 45–60 min, while the LVHIT trained at 70–85% of VO2max for 25–40 min. Both training protocols were equated by caloric expenditure. HRV, pulse wave velocity (PWV), hemodynamic variables, and body composition were measured before and after 12 weeks. Results: Both protocols (i.e., HVMIT and LVHIT) significantly increased the SD of normal sinus beat intervals (SDNN) and high-frequency (HF) bands (p < 0.05) after 12 weeks. Whereas the low-frequency (LF)-HF ratio decreased significantly in both training protocols (p < 0.05); however, these changes were significantly greater in the LVHIT protocol (p < 0.05). Furthermore, the root mean square of successive RR interval differences (RMSSD) significantly increased only in the LVHIT (p < 0.05). Moreover, a significant decrease in LF and PWV was only observed following the LVHIT protocol (p < 0.05). Some measures of HRV and PWV were significantly correlated (r = 0.275–0.559; p < 0.05). Conclusion: These results show that the LVHIT protocol was more efficient for improving HRV variables and PWV than the HVMIT protocol after 12 weeks of continuous running training. Interestingly, changes in some HRV parameters were related to changes in PWV. Further studies should elaborate on the link between central and peripheral cardiovascular adaptations after continuous and intermittent training regimens differing in intensity

MAB21L1 loss of function causes a syndromic neurodevelopmental disorder with distinctive cerebellar, ocular, craniofacial and genital features (COFG syndrome).

BACKGROUND: Putative nucleotidyltransferase MAB21L1 is a member of an evolutionarily well-conserved family of the male abnormal 21 (MAB21)-like proteins. Little is known about the biochemical function of the protein; however, prior studies have shown essential roles for several aspects of embryonic development including the eye, midbrain, neural tube and reproductive organs. OBJECTIVE: A homozygous truncating variant in MAB21L1 has recently been described in a male affected by intellectual disability, scrotal agenesis, ophthalmological anomalies, cerebellar hypoplasia and facial dysmorphism. We employed a combination of exome sequencing and homozygosity mapping to identify the underlying genetic cause in subjects with similar phenotypic features descending from five unrelated consanguineous families. RESULTS: We identified four homozygous MAB21L1 loss of function variants (p.Glu281fs*20, p.Arg287Glufs*14 p.Tyr280* and p.Ser93Serfs*48) and one missense variant (p.Gln233Pro) in 10 affected individuals from 5 consanguineous families with a distinctive autosomal recessive neurodevelopmental syndrome. Cardinal features of this syndrome include a characteristic facial gestalt, corneal dystrophy, hairy nipples, underdeveloped labioscrotal folds and scrotum/scrotal agenesis as well as cerebellar hypoplasia with ataxia and variable microcephaly. CONCLUSION: This report defines an ultrarare but clinically recognisable Cerebello-Oculo-Facio-Genital syndrome associated with recessive MAB21L1 variants. Additionally, our findings further support the critical role of MAB21L1 in cerebellum, lens, genitalia and as craniofacial morphogenesis

Mechanical properties and microstructure of VPS and HVOF CoNiCrAlY coatings

HVOF and VPS coatings were sprayed using a Praxair (CO-210-24) CoNiCrAlY powder. Free standing coatings underwent vacuum annealing at different temperatures for times of up to 840h. Feedstock powder, as-sprayed and annealed coatings were characterised by SEM, EDS and XRD. The hardness and Young’s modulus of as-sprayed and annealed HVOF and VPS coatings were measured, including determination of Young’s moduli of the individual phases via nanoindentation and measurement of Young’s moduli of coatings at temperatures up to 500°C. The Eshelby inclusion model was used to investigate the effect of microstructure on the coatings’ mechanical properties. The sensitivity of the mechanical properties to microstructural details was confirmed. Young’s modulus was constant to ~200°C then decreased with increasing measurement temperature. Annealing increased Young’s modulus due to a combination of decreased porosity and β volume fraction. Oxide stringers in the HVOF coating maintained its higher hardness than the VPS coating even after annealing

pSESYNTH project: Community mobilization for a multi-disciplinary paleo database of the Global South

How to enhance paleoscientific research, collaboration and application in the Global South? The INQUA-funded multi-year pSESYNTH project envisions the first multi-disciplinary Holocene paleo database through a collaborative vision for past human-environmental systems in the Global South, and their future sustainability