Growth of Oriented Au Nanostructures: Role of Oxide at the Interface

We report on the formation of oriented gold nano structures on Si(100) substrate by annealing procedures in low vacuum (\approx10-2 mbar) and at high temperature (\approx 975^{\circ} C). Various thicknesses of gold films have been deposited with SiOx (using high vacuum thermal evaporation) and without SiOx (using molecular beam epitaxy) at the interface on Si(100). Electron microscopy measurements were performed to determine the morphology, orientation of the structures and the nature of oxide layer. Interfacial oxide layer, low vacuum and high temperature annealing conditions are found to be necessary to grow oriented gold structures. These gold structures can be transferred by simple scratching method.Comment: 13 pages, 3 figures, Accepted in J. Appl. Phy

Transcriptome Analysis for Abiotic Stresses in Rice (<em>Oryza sativa</em> L.)

Rice, a model monocot system, belongs to the family Poaceae and genus Oryza. Rice is the second largest produced cereal and staple food crop fulfilling the demand of half the world’s population. Though rice demand is growing exponentially, its production is severely affected by variable environmental changes. The various abiotic factors drastically reduce the rice plant growth and yield by affecting its different growth stages. To fulfill the growing demand of rice, it is imperative to understand its molecular responses during stresses and to develop new varieties to overcome the stresses. Earlier, the microarray experiments have been used for the identification of coexpressive gene networks during various conditions in crop plants. Though the microarray experiments provided very useful information, the unviability of genome-wide information did not provide complete information about the regulatory gene networks involved in the stress response. The advancement of molecular techniques provided breakthrough to understanding the complex regulatory gene networks and their signaling pathways during stresses. The high-throughput RNA sequencing data have opened the floodgate of transcriptome data in rice. Here we have summarized some of the transcriptome data for abiotic molecular responses in rice, which further help to understand their complex regulatory mechanism

Antimicrobial Evaluation of Dichloro Chromene Isoxazoles and Isoxazoline Derivatives

In the present study, the synthesized benzopyran isoxazolines and isoxazoles were evaluated for antibacterial activity by Agar well diffusion method against multi drug resistance (MDR) clinical isolates of five bacterial strains.i.e., Staphylococcus sp. (2413), Enterococcus sp. (2449), and Escherichia sp. (2461), Acinetobacter sp. (2457) and Serratia sp (2442). MIC (Minimum inhibitory concentration) and MBC (Minimum bactericidal concentration) of the potent derivatives were calculated and tabulated. A partial structure activity relationship (SAR) was done on the basis of microbial inhibition data. Compounds 3, 6, 8, 9, 11, 14 and 20 were the most promising antibacterial molecule. Compound 3, the precursor of isoxazole / isoxazoline found to be most active antibacterial candidate as well as bactericidal too

Identification and quantification of biological active constituents of Amritarishta, a herbal formulation

Herbal formulations have been used by Indian and Chinese traditional systems of medication for a long time. Amritarishtais one of the herbal formulations that possess various biological activity viz., antioxidant, anticancer, analgesic, antipyretic,antidiabetic, etc. The active constituents include gallic acid, tannic acid, piperine, and quercetin, etc. Ethanolic extract of theformulation was analysed and quantified. Rf (Retardation factor), functional groups and amount of some of the major chemicalconstituents were analysed by TLC, FTIR, LC/MS, HPTLC and HPLC, respectively. LC/MS results reveal the presence ofquercetin, piperine, tannic acid and gallic acid in the formulation. With the help of HPTLC and HPLC, the quantity of 4chemical constituents in the formulation was estimated. This type of study is completely new to herbal research

One pot synthesis of luminescent Mn doped ZnSe nanoparticles and their silica based water dispersible formulation for targeted delivery of doxorubicin

348-355The manganese doped zinc selenide nanoparticles (ZnSe:Mn NPs) have been synthesized by thermolysis method using oleic acid and oleylamine as capping agents, and 1-octadecene as solvent. Coating of mesoporous silica is done on ZnSe:Mn (ZnSe:Mn@mSilica) which is further functionalized with amine functional groups by treating with (3-aminopropyl)trimethoxysilane. Further pegylation is done to achieve water dispersibility by conjugating carboxyl groups of poly(ethylene glycol) diacid with the amine groups. These pegylated NPs are subsequently treated with ethylenediamine followed by acrylic acid. Conjugation of tris-(hydroxymethyl-aminomethane) is performed by Michael-type addition reaction to afford ZnSe:Mn@mSilica-PEG-Tris-OH. These tris functionalized NPs have exhibited broad emission ranging from 590-620 nm that is an indicative for their suitability in diagnosis and monitoring progress of cancer treatment. To explore the usefulness of increased surface area because of mesoporosity, doxorubicin is loaded on ZnSe:Mn@mSilica-PEG-Tris-OH NPs through silyl ether linkage and evaluated for cytotoxicity against WEHI-164 mouse fibrosarcoma and RAJI human hematopoietic origin cancer cell lines. A decrease in 12% of cell viability of WEHI-164 cells while 30% decrease in RAJI cell lines (IC50 ≈ 45 nM) are observed. This shows that our formulation has more cytotoxic in RAJI cancer cell lines than that of WEHI-164 cancer cells. These results reveal that the formulation has potential for the application in drug delivery and diagnosis in chemotherapeutics

One pot synthesis of luminescent Mn doped ZnSe nanoparticles and its silica based water dispersible formulation for targeted delivery of doxorubicin

The manganese doped zinc selenide nanoparticles (ZnSe:Mn NPs) were synthesized by thermolysis method using oleic acid and oleylamine as a capping agent, 1-octadecene as solvent. Coating of mesoporous silica was done on ZnSe:Mn (ZnSe:Mn@mSilica) which was further functionalized with amine functional groups by treating with (3-aminopropyl)trimethoxysilane. Further pegylation was done to achieve water dispersibility by conjugating carboxyl groups of poly(ethylene glycol) diacid with the amine groups. These pegylated NPs were subsequently treated with ethylenediamine followed by acrylic acid. Conjugation of tris-(hydroxymethyl-aminomethan) was performed by Michael-type addition reaction to afford ZnSe:Mn@mSilica-PEG-Tris-OH. These TRIS functionalized NPs exhibited broad emission ranging from 590-620 nm that is an indicative for their suitability in diagnosis and monitoring progress of cancer treatment. To explore the usefulness of increased surface area because of mesoporosity, doxorubicin was loaded on ZnSe:Mn@mSilica-PEG-Tris-OH NPs through silyl ether linkage and evaluated for cytotoxicity against WEHI-164 mouse fibrosarcoma and RAJI human hematopoietic origin cancer cell lines. A decrease in 12 % of cell viability of WEHI-164 cells while 30% decrease in RAJI cell lines (IC50 ≈ 45 nM) were observed.  This shows that our formulation has more cytotoxic in RAJI cancer cell lines than that of WEHI-164 cancer cells. These results revealed that the formulation has potential for the application in drug delivery and diagnosis in chemotherapeutics

MUSiC : a model-unspecific search for new physics in proton-proton collisions at root s=13TeV

Results of the Model Unspecific Search in CMS (MUSiC), using proton-proton collision data recorded at the LHC at a centre-of-mass energy of 13 TeV, corresponding to an integrated luminosity of 35.9 fb(-1), are presented. The MUSiC analysis searches for anomalies that could be signatures of physics beyond the standard model. The analysis is based on the comparison of observed data with the standard model prediction, as determined from simulation, in several hundred final states and multiple kinematic distributions. Events containing at least one electron or muon are classified based on their final state topology, and an automated search algorithm surveys the observed data for deviations from the prediction. The sensitivity of the search is validated using multiple methods. No significant deviations from the predictions have been observed. For a wide range of final state topologies, agreement is found between the data and the standard model simulation. This analysis complements dedicated search analyses by significantly expanding the range of final states covered using a model independent approach with the largest data set to date to probe phase space regions beyond the reach of previous general searches.Peer reviewe

Measurement of prompt open-charm production cross sections in proton-proton collisions at root s=13 TeV

The production cross sections for prompt open-charm mesons in proton-proton collisions at a center-of-mass energy of 13TeV are reported. The measurement is performed using a data sample collected by the CMS experiment corresponding to an integrated luminosity of 29 nb(-1). The differential production cross sections of the D*(+/-), D-+/-, and D-0 ((D) over bar (0)) mesons are presented in ranges of transverse momentum and pseudorapidity 4 < p(T) < 100 GeV and vertical bar eta vertical bar < 2.1, respectively. The results are compared to several theoretical calculations and to previous measurements.Peer reviewe