28 research outputs found
Phenology of deer ked (Lipoptena cervi) host-seeking flight activity and its relationship with prevailing autumn weather
Humanised transgenic mice are resistant to chronic wasting disease prions from Norwegian reindeer and moose
Chronic wasting disease (CWD) is the transmissible spongiform encephalopathy or prion disease affecting cervids. In 2016 the first cases of CWD were reported in Europe in Norwegian wild reindeer and moose. The origin and zoonotic potential of these new prion isolates remain unknown. In this study to investigate zoonotic potential we inoculated brain tissue from CWD-infected Norwegian reindeer and moose into transgenic mice overexpressing human prion protein. After prolonged post-inoculation survival periods no evidence for prion transmission was seen suggesting that the zoonotic potential of these isolates is low
Gastrointestinal parasites in an isolated Norwegian population of wild red deer (Cervus elaphus)
From food defence to food supply chain integrity
Purpose – Consumer confidence in the European food industry has been shaken by a number of recent
scandals due to food fraud and accidental contamination, reminding the authors that deliberate incidents can
occur. Food defence methods aim to prevent or mitigate deliberate attacks on the food supply chain but are
not a legal requirement. The purpose of this paper is to discuss how proactive and reactive food defence
practices can help prevent or mitigate malicious attacks on the food chain and also food fraud, food crime and
food safety. The authors look at how food defence differs from food safety and how it contributes to food
supply chain integrity.
Design/methodology/approach – Food defence has been the focus of two different EU FP7 security
projects, EDEN and SNIFFER. Food industry stakeholders participated in workshops and demonstrations on
food defence and relevant technology was tested in different food production scenarios.
Findings – Food industry end-users reported a lack of knowledge regarding food defence practices. They
wished for further guidelines and training on risk assessment as well as access to validated test methods.
Novel detection tools and methods showed promise with authentication, identification, measurement,
assessment and control at multiple levels of the food supply chain prior to distribution and retail.
Practical implications – The prevention of a contamination incident, prior to retail, costs less than dealing
with a large foodborne disease outbreak. Food defence should therefore be integral to food supply chain
integrity and not just an afterthought in the wake of an incident.
Originality/value – It is argued that food defence practices have a vital role to play across the board in
unintentional and intentional food contamination incidents. The application of these methods can help ensure
food supply chain integrity.European Union (EU) Seventh Framework Programme
projects (FP7/2007-2013): EDEN: End-User Driven Demo for CBRNe, under Grant
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Food supply
chain integrity
Downloaded by UNIVERSIDAD DE BURGOS At 07:57 06 January 2017 (PT)
Agreement no. 313077, and SNIFFER: Sensory devices network for food supply chain
security, under Grant Agreement no. 312411
In silico exploration of Red Sea Bacillus genomes for natural product biosynthetic gene clusters
Background: The increasing spectrum of multidrug-resistant bacteria is a major global public health concern, necessitating discovery of novel antimicrobial agents. Here, members of the genus Bacillus are investigated as a potentially attractive source of novel antibiotics due to their broad spectrum of antimicrobial activities. We specifically focus on a computational analysis of the distinctive biosynthetic potential of Bacillus paralicheniformis strains isolated from the Red Sea, an ecosystem exposed to adverse, highly saline and hot conditions. Results: We report the complete circular and annotated genomes of two Red Sea strains, B. paralicheniformis Bac48 isolated from mangrove mud and B. paralicheniformis Bac84 isolated from microbial mat collected from Rabigh Harbor Lagoon in Saudi Arabia. Comparing the genomes of B. paralicheniformis Bac48 and B. paralicheniformis Bac84 with nine publicly available complete genomes of B. licheniformis and three genomes of B. paralicheniformis, revealed that all of the B. paralicheniformis strains in this study are more enriched in nonribosomal peptides (NRPs). We further report the first computationally identified trans-acyltransferase (trans-AT) nonribosomal peptide synthetase/polyketide synthase (PKS/ NRPS) cluster in strains of this species. Conclusions:B. paralicheniformis species have more genes associated with biosynthesis of antimicrobial bioactive compounds than other previously characterized species of B. licheniformis, which suggests that these species are better potential sources for novel antibiotics. Moreover, the genome of the Red Sea strain B. paralicheniformis Bac48 is more enriched in modular PKS genes compared to B. licheniformis strains and other B. paralicheniformis strains. This may be linked to adaptations that strains surviving in the Red Sea underwent to survive in the relatively hot and saline ecosystems
Developments of electric cars and fuel cell hydrogen electric cars
The world continues to strive in the search for clean power sources to run the millions of different vehicles on the road on daily basis as they are the main contributors to toxic emissions releases from internal combustion engines to the atmosphere. These toxic emissions contribute to climate change and air pollution and impact negatively on people's health. Fuel cell devices are gradually replacing the internal combustion engines in the transport industry. Some notable challenges of the PEMFC technology are discussed in this paper. High costs, low durability and hydrogen storage problems are some of the major obstacles being examined in this investigation. The paper explores the latest advances in electric cars technology and their design specifications. The study also compares the characteristics and the technologies of the three types of electric cars now available in the market.interna
Echinococcus multilocularis-adaptation of a worm egg isolation procedure coupled with a multiplex PCR assay to carry out large-scale screening of red foxes (Vulpes vulpes) in Norway
Echinococcus multilocularis, causing alveolar echinococcosis in humans, is a highly pathogenic emerging zoonotic disease in central Europe. The gold standard for the identification of this parasite in the main host, the red fox, namely identification of the adult parasite in the intestine at necropsy, is very laborious. Copro-enzyme-linked immunosorbent assay (ELISA) with confirmatory polymerase chain reaction (PCR) has been suggested as an acceptable alternative, but no commercial copro-ELISA tests are currently available and an in-house test is therefore required. Published methods for taeniid egg isolation and a multiplex PCR assay for simultaneous identification of E. multilocularis, E. granulosus and other cestodes were adapted to be carried out on pooled faecal samples from red foxes in Norway. None of the 483 fox faecal samples screened were PCR-positive for E. multilocularis, indicating an apparent prevalence of between 0% and 1.5%. The advantages and disadvantages of using the adapted method are discussed as well as the results pertaining to taeniid and non-taeniid cestodes as identified by multiplex PCR
Gastrointestinal parasites in an isolated Norwegian population of wild red deer (Cervus elaphus)
BACKGROUND: Thirteen red deer (Cervus elaphus), culled from the isolated population at the Mongstad Oil Refinery, Norway, were investigated for gastrointestinal helminths. These animals, enclosed by the refinery fence, do not have contact with other ruminants and have a high population density considering the available browsing area (1 km(2)) within the refinery site (3 km(2)). The population was estimated to be 110-130 at the time of culling. RESULTS: The helminth fauna among these sampled red deer was enumerated and species were identified based on morphology. Ostertagia leptospicularis/O. kolchida was detected in 83% [CI 55 - 95%], Spiculopteragia spiculoptera/S. mathevossiani in 92% [CI 65 - 99%] and Trichostrongylus axei in 42%, [CI 19 - 68%] of the abomasa examined. Characterisation of the intestinal parasite fauna revealed Capillaria bovis, Cooperia oncophora, Oesophagostomum venulosum, Trichuris globulosa and tapeworm fragments (presumed anoplocephalids) in seven individuals. Only one calf had an infection with more than one intestinal helminth (tapeworm fragment and Trichuris globulosa). The remaining six deer had single species intestinal infections. No significant age related trends were seen, with the exception of higher intensity of infection of T. axei in yearlings relative to other age classes. Assessment of abomasal parasite burden and body condition revealed no significant trends. In calves, statistically non-significant correlation was seen between increased parasite burden and decreased slaughter weight, whilst the opposite was seen in adults with the heaviest adults exhibiting the higher burdens. Given the small sample size the trends that were seen need further investigation. The parasite burden was aggregated with three adult red deer harbouring 75% of the total abomasal parasite count. CONCLUSION: This isolated population was parasitised by a reduced subset of gastrointestinal nematodes typical of this cervid across an extensive geographic range in Eurasia. The intensity and abundance of abomasal nematodes was higher in this isolated population than reported in similar studies of red deer populations across Europe