Guidelines and Recommendations on Yeast Cell Death Nomenclature

Elucidating the biology of yeast in its full complexity has major implications for science, medicine and industry. One of the most critical processes determining yeast life and physiology is cellular demise. However, the investigation of yeast cell death is a relatively young field, and a widely accepted set of concepts and terms is still missing. Here, we propose unified criteria for the definition of accidental, regulated, and programmed forms of cell death in yeast based on a series of morphological and biochemical criteria. Specifically, we provide consensus guidelines on the differential definition of terms including apoptosis, regulated necrosis, and autophagic cell death, as we refer to additional cell death routines that are relevant for the biology of (at least some species of) yeast. As this area of investigation advances rapidly, changes and extensions to this set of recommendations will be implemented in the years to come. Nonetheless, we strongly encourage the authors, reviewers and editors of scientific articles to adopt these collective standards in order to establish an accurate framework for yeast cell death research and, ultimately, to accelerate the progress of this vibrant field of research

Guidelines and recommendations on yeast cell death nomenclature

Elucidating the biology of yeast in its full complexity has major implications for science, medicine and industry. One of the most critical processes determining yeast life and physiology is cel-lular demise. However, the investigation of yeast cell death is a relatively young field, and a widely accepted set of concepts and terms is still missing. Here, we propose unified criteria for the defi-nition of accidental, regulated, and programmed forms of cell death in yeast based on a series of morphological and biochemical criteria. Specifically, we provide consensus guidelines on the differ-ential definition of terms including apoptosis, regulated necrosis, and autophagic cell death, as we refer to additional cell death rou-tines that are relevant for the biology of (at least some species of) yeast. As this area of investigation advances rapidly, changes and extensions to this set of recommendations will be implemented in the years to come. Nonetheless, we strongly encourage the au-thors, reviewers and editors of scientific articles to adopt these collective standards in order to establish an accurate framework for yeast cell death research and, ultimately, to accelerate the pro-gress of this vibrant field of research

Relative tissue expression of homologous torsinB correlates with the neuronal specific importance of DYT1 dystonia-associated torsinA

A three base-pair deletion in the widely expressed TOR1A gene causes the childhood onset, neurological disease of DYT1 dystonia. Mouse Tor1a gene knockout also specifically affects the developing nervous system. However, in both cases, the basis of neuronal tissue specificity is unknown. TorsinA is one of four predicted mammalian torsin ATPases associated with assorted cellular activities (AAA+) proteins, raising the possibility that expression of a functionally homologous torsin compensates for torsinA loss in non-neuronal tissues. We find that all four mammalian torsins are endoplasmic reticulum resident glycoproteins. TorsinA, torsinB and torsin2 are all present in large M(r) complexes, which suggests that each assembles into an oligomeric AAA+ enzyme. Introducing a mutation (WB(EQ)) that typically stabilizes AAA+ proteins in a substrate-bound state causes torsinA and torsinB to associate with a shared nuclear envelope (NE) binding partner and this NE localization requires the torsinA interacting protein, lamina associated polypeptide 1. Although torsin proteins are widely expressed in the adult mouse, we identified that embryonic neuronal tissues contain relatively low torsinB levels. Therefore, our results reveal that torsinB expression inversely correlates with the cell and developmental requirement for torsinA. In conclusion, multiple cell types appear to utilize torsin AAA+ proteins and differential expression of torsinB may contribute to both the neuronal specific importance of torsinA and the symptom specificity of DYT1 dystonia.status: publishe

The State of the Dry Forest Zone and its Communities

94 p.The Dry Forest Zone is a region of eastern Oregon and northern California with challenging market conditions and high levels of poverty and unemployment. However, local entrepreneurship, collaboration, and commitment to integrated economic development and natural resource management in the zone are strong. In the past decade, the scope of community-based nonprofits, integrated biomass utilization businesses, and new networks has increased, fostering sustainable forest stewardship at an increasingly regional scale. The geography and climate of the zone support dry forests of pine and mixed conifer with fire regimes that are departed from their historical range of variability. These forests are prone to wildfire hazards and in need of active management to restore more diverse and variable-aged structures. As 68 percent of the land in the zone is public, the communities of this region rely on the economic and ecological productivity of these federal forests. The number of sawmills that once provided high levels of primary processing capacity and employment has shrunk to nine mills in the zone. More forest-related employment is now forestry support work, including activities such as firefighting, pest control, and thinning. Poverty and unemployment have increased, with estimated poverty levels in 2007 of over 15 percent in ten of the fifteen counties. Through the Dry Forest Zone project, we have an opportunity to build on the local strengths of this region and overcome these ecological and socioeconomic challenges.Financial support was provided the U.S. Endowment for Forestry and Communities, USDA Rural Development, and the Ford Foundation

Relative tissue expression of homologous torsins can explain the neuronal specific importance of DYT1 dystonia-associated torsinA

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Local benefits from land management : a strategy for measuring performance

3 pagesThe Forest Service faces a long-standing challenge of reporting accomplishments in ways that Congress and the public find compelling. Over the past several years, the Forest Service has made significant improvements to its accountability system. The Forest Service plays an important role in rural economies, especially in the rural West. However, the Forest Service does not track the economic effects that Forest Service work has on local communities. Describing the Forest Service’s impact on rural communities could increase understanding and support for the agency’s work. The Forest Service can use data that it already collects to track initial measures of the economic effects of its management on local communities.This briefing paper was made possible with funding from the USDA Forest Service, Pacific Northwest Research Station, and the Compton Foundation