Correlação de imagens digitais

Resumo: Esta dissertação tem por objetivo elaborar a automação do processo de medição de alvo s ou feições imageados com a câmara de TV-HR-600. Neste processo de medição são empregados dois métodos de correlação . O primeiro método consiste em aplicar uma função de correlação a janelas de um estereopar de imagens digitais , para obter a correlação de áreas ao nível do pix el. O segundo método trata a correlação encontrada empregando a t e o r ia dos mínimos quadrados a um modelo matemático que remove as distorções geométricas e as diferenças sistemática s radiometricas inerente s ao processo de aquisição da imagem refinando o processo e elevando a precisão

Trans-Stilbenes in Commercial Grape Juices: Quantification Using HPLC Approaches

Trans-stilbenes belong to the group of polyphenolic phytoalexins, and occur in many plant foods. These compounds have received great attention by researchers due to their well-known beneficial health effects. In the present study a chromatographic method that comprises the use of variable wavelength (VWD) and fluorescence (FLD) detectors in series for the analysis of trans-stilbenes is presented. The relation of peak-area obtained with both detectors is proposed as an alternative and complementary approach for the rapid identification of these phenolic compounds. The proposed method was applied to determine trans-stilbenes in commercial fruit juices. Trans-piceid was the most common trans-stilbene found in the samples analyzed. The method was validated in terms of linearity, sensitivity and repeatability. Appropriate sensitivity and good linearity (r2 > 0.9991) were achieved.This work was financed under grant GRC2014/012 for consolidating and structuring of competitive research units do Galician University System, SpainS

Variación de compuestos bioactivos en algas marinas Himanthalia elongata deshidratadas expuestas a distintos procesos culinarios

Different studies have already demonstrated a direct relationship between seaweed consumption and the prevention and/or improvement in the treatment of diseases related to oxidative stress. Algae have been used, therefore, in the investigation of bioactive compounds, with the aim of developing new medicines and healthy foods. This article presents the results of the analysis of vitamin C, vitamin E, fucoxanthin, chlorophyll a, pheophytin a, lutein, β-carotene, total polyphenol content, and antioxidant activity in Himanthalia elongata dehydrated, hydrated by immersion, boiled, and steamed. These results contribute to a better understanding of the bioactive compounds’ behavior when subjected to different culinary methods and reaffirms the potential of H. elongata as interesting food in our dietDiferentes estudios han demostrado una relación directa entre el consumo de algas y la prevención y/o mejora en el tratamiento de enfermedades relacionadas con el estrés oxidativo. En las algas se ha investigado la presencia de compuestos bioactivos, con el objetivo de desarrollar nuevos medicamentos y alimentos para la salud. Este trabajo presenta los resultados del análisis de vitamina C, vitamina E, fucoxantina, clorofila a, feofitina, luteína, β-caroteno, contenido total de polifenoles y actividad antioxidante en Himanthalia elongata deshidratada, hidratada por inmersión, hervida y al vapor. Estos resultados contribuyen a una comprensión mejor del comportamiento de los compuestos bioactivos cuando la H. elongata se somete a diferentes métodos culinarios y confirma su interés potencial como alimento de nuestra dietaThis work was financed under project no. 09TAL023203PR from Xunta de GaliciaS

Cambios en compuestos bioactivos y actividad antioxidante de algas despue´s del proceso de cocinado

Wakame and Kombu seaweed, both fresh and reconstituted for consumption, were analyzed by HPLC for the determination of the content of ascorbic acid, vitamin E, β-carotene, lutein, fucoxanthin, chlorophyll a and pheophytin a; total polyphenol was done by Folin–Ciocalteau method and antioxidant activity by the DPPH test. Different behaviors have been observed on the contents of the bioactive compounds analyzed. Significant changes on ascorbic acid, β-carotene, lutein, fucoxanthin, chlorophyll a, pheophytin a, vitamin E, total polyphenols and antioxidant activity have been observed after the reconstitution of Wakame, whereas Kombu showed no significative variation of fucoxanthin, Vitamin E, total polyphenols and antioxidant activity. A high correlation between antioxidant activity and the above compounds was observedEn este trabajo se ha determinado ácido ascórbico, vitamina E, β-caroteno, luteína, fucoxantina, clorofila a y feofitina a mediante HPLC, polifenoles totales por el método Folin–Ciocalteau y actividad antioxidante mediante la prueba DPPH, en algas Wakame y Kombu, frescas y reconstituidas para el consumo. Se han observado cambios significativos en el contenido de ácido ascórbico, vitamina E, β-caroteno, luteína, fucoxantina, clorofila a y feofitina a, polifenoles totales y actividad antioxidante después de la reconstitución del Wakame, mientras que el Kombu no presenta variaciones significativas para la fucoxantina, vitamina E, polifenoles totales y actividad antioxidante. Se ha encontrado una alta correlación entre los compuestos bioactivos analizados y la actividad antioxidanteThis work was financed under project no. 09TAL023203PR from Xunta de GaliciaS

Swimming performance of Bradyrhizobium diazoefficiens is an emergent property of its two flagellar systems

Many bacterial species use flagella for self-propulsion in aqueous media. In the soil, which is a complex and structured environment, water is found in microscopic channels where viscosity and water potential depend on the composition of the soil solution and the degree of soil water saturation. Therefore, the motility of soil bacteria might have special requirements. An important soil bacterial genus is Bradyrhizobium, with species that possess one flagellar system and others with two different flagellar systems. Among the latter is B. diazoefficiens, which may express its subpolar and lateral flagella simultaneously in liquid medium, although its swimming behaviour was not described yet. These two flagellar systems were observed here as functionally integrated in a swimming performance that emerged as an epistatic interaction between those appendages. In addition, each flagellum seemed engaged in a particular task that might be required for swimming oriented toward chemoattractants near the soil inner surfaces at viscosities that may occur after the loss of soil gravitational water. Because the possession of two flagellar systems is not general in Bradyrhizobium or in related genera that coexist in the same environment, there may be an adaptive tradeoff between energetic costs and ecological benefits among these different species.Fil: Quelas, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Althabegoiti, Maria Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Jiménez Sánchez, Celia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina. Instituto de Recursos Naturales y Agrobiología de Sevilla; EspañaFil: Melgarejo, Augusto. Universidad Nacional de La Plata. Facultad de Ingeniería; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Marconi, Veronica Iris. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; ArgentinaFil: Mongiardini, Elias Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Trejo, Sebastian Alejandro. Universitat Autònoma de Barcelona; España. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Mengucci, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Ortega Calvo, José Julio. Instituto de Recursos Naturales y Agrobiología de Sevilla; EspañaFil: Lodeiro, Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentin

STRATEGY FOR EXTRACTION OF FOURSQUARE’S SOCIAL MEDIA GEOGRAPHIC INFORMATION THROUGH DATA MINING

This aim of this paper is the acquisition of geographic data from the Foursquare application, using data mining to perform exploratory and spatial analyses of the distribution of tourist attraction and their density distribution in Rio de Janeiro city. Thus, in accordance with the Extraction, Transformation, and Load methodology, three research algorithms were developed using a tree hierarchical structure to collect information for the categories of Museums, Monuments and Landmarks, Historic Sites, Scenic Lookouts, and Trails, in the foursquare database. Quantitative analysis was performed of check-ins per neighborhood of Rio de Janeiro city, and kernel density (hot spot) maps were generated The results presented in this paper show the need for the data filtering process — less than 50% of the mined data were used, and a large part of the density of the Museums, Historic Sites, and Monuments and Landmarks categories is in the center of the city; while the Scenic Lookouts and Trails categories predominate in the south zone. This kind of analysis was shown to be a tool to support the city's tourist management in relation to the spatial localization of these categories, the tourists’ evaluations of the places, and the frequency of the target public

Composición química del caparazón del cangrejo de las nieves (Chionoecetes opilio)

The snow crab (Chionoecetes opilio) shells have potential important economical and industrial applications due to theirchemical composition. They have a high content of protein (34.2% dw) and essential amino acids; they also have fat (17.1% dw), with a high proportion of ω3 polyunsaturated fatty acids. About 28.5% dw corresponds to ash (calcium, phosphorous, and magnesium are the major minerals). The mean concentrations of vitamin E, astaxantin, and β-carotene were 23.3, 9.49, and 0.2 mg/100 g dw, respectively. Results presented in this study indicate that snow crab by-products may serve as excellent nutritional components for future applications in the health and food sectorsLos caparazones del cangrejo de las nieves (Chionoecetes opilio) pueden tener aplicaciones industriales y económicas potencialmente importantes debido a su composición química. En su composición destaca el elevado contenido en proteínas (34,2% ms) y aminoácidos esenciales, en lípidos totales (17,1% ms), con una elevada proporción de ácidos grasos poliinsaturados ω3, en cenizas (28% (ms), principalmente calcio, fosfatos y magnesio. La concentración media de vitamina E es de 23,3 mg /100 g ms, de astaxantina 9,49 mg /100 g ms, de β-caroteno 0,2 mg /100 g ms. El alto contenido en nutrientes, puesto de manifiesto en este estudio, indica que los productos de desecho del cangrejo de las nieves pueden ser utilizados como productos de alto valor añadido para su aplicación en suplementación de dietas para alimentación humana y acuiculturaThis work was supported by the Consellería de Educación e Ordenación Universitaria da Xunta de Galicia (Project PGIDIT03BTF00701CT). Technical assistance of Patricia Ferraces Casais is highly appreciatedS

Avaliação Dos Critérios De Elegibilidade Do Programa Territórios Da Cidadania

O Programa Territórios da Cidadania (PTC) é uma das mais relevantes políticas de desenvolvimento territorial vigentes no Brasil, principalmente no que tange sua abrangência e importância. Trata-se de uma política pública social que apresenta escopo diferenciado, com enfoque territorial, gestão social e interministerial. Apesar de o PTC ser oriundo de um processo evolutivo de políticas públicas como: Programa Nacional de Fortalecimento da Agricultura Familiar (Pronaf) e Programa de Desenvolvimento Sustentável dos Territórios Rurais (Pronat), este ainda apresenta certos gargalos, como a definição de critérios para a escolha dos territórios. Neste sentido, o artigo apresenta metodologia estatística com o objetivo de avaliar os critérios de elegibilidade e o grau de importância desses critérios para que um município seja atendido pelo programa, a partir de indicadores proxies aos critérios definidos em decreto. Como resultado, critérios como densidade demográfica, existência de quilombos e população indígena não apresentaram o protagonismo esperado. Já o Índice de Desenvolvimento Humano apresentou-se como principal critério de elegibilidade, mostrando que o indicador se mantém relevante para o ciclo de monitoramento e avaliação de políticas públicas brasileiras. A agricultura familiar, um dos principais pilares do PTC e de seus programas antecessores, também se mostrou importante, mantendo a coerência do escopo e objetivos do programa.54459961

Codesign for People with Aphasia Through Tangible Design Languages

Codesign techniques encourage designers and end-users to work together in the creation of design solutions, but often make assumptions about the ways in which participants will be able to communicate. This can lead to the unwitting exclusion of people with communication impairments from the design of technologies that have the potential to transform their lives. This paper reports our research into codesign techniques for people whose communication skills are impaired. A variety of techniques were explored on two projects; some were adaptations of existing codesign techniques, others were created specially. In both cases, the emphasis was on creating tangible design languages. The results illustrate how people with communication impairments can be given a voice in design and demonstrate the benefits of doing so

Functional plasticity of antibacterial EndoU toxins.

Bacteria use several different secretion systems to deliver toxic EndoU ribonucleases into neighboring cells. Here, we present the first structure of a prokaryotic EndoU toxin in complex with its cognate immunity protein. The contact-dependent growth inhibition toxin CdiA-CTSTECO31 from Escherichia coli STEC_O31 adopts the eukaryotic EndoU fold and shares greatest structural homology with the nuclease domain of coronavirus Nsp15. The toxin contains a canonical His-His-Lys catalytic triad in the same arrangement as eukaryotic EndoU domains, but lacks the uridylate-specific ribonuclease activity that characterizes the superfamily. Comparative sequence analysis indicates that bacterial EndoU domains segregate into at least three major clades based on structural variations in the N-terminal subdomain. Representative EndoU nucleases from clades I and II degrade tRNA molecules with little specificity. In contrast, CdiA-CTSTECO31 and other clade III toxins are specific anticodon nucleases that cleave tRNAGlu between nucleotides C37 and m2 A38. These findings suggest that the EndoU fold is a versatile scaffold for the evolution of novel substrate specificities. Such functional plasticity may account for the widespread use of EndoU effectors by diverse inter-bacterial toxin delivery systems