Multiparametric Highly Sensitive Chemiluminescence Immunoassayfor Quantification of b-Lactam-Specific Immunoglobulin E

This document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.analchem.0c03020[EN] beta-lactams (BLCs) are the most widely used antibiotics and consequently the most common cause of drug allergy in the world. The diagnosis of drug allergy is complex and represents a serious challenge that includes a wide variety of methods. In vitro tests are based on the immunological determination of allergen-specific IgE, but the tests in the market lack the required sensitivity and specificity. In addition, the large sample volume, long incubation times, and single-plex configuration have brought their use into question to complement the clinical information. Here, we report a chemiluminescence immunoassay (CLIA) for multiparametric quantification of specific IgE to penicillin G, penicillin V, amoxicillin, and piperacillin, using histone H1 as a carrier. The developed CLIA allowed the determination of BLC-specific IgE below 0.1 IU/mL, thus allowing identification of allergic patients with better sensitivity, using only 25 mu L of a sample (serum). The immunoassay was successfully applied in a cohort of 140 human serum samples, showing good sensitivity (64.6%) as well as specificity (100%), which significantly improve the predictive character of existing BLC-allergy in vitro tests.P.Q.C. acknowledges financial support from Generalitat Valenciana through the research staff-training program (GVA ACIF/2018/173). This research has been funded by the H2020 Program (project COBIOPHAD, grant agreement No. 688448), which is an initiative of the Photonics PPP (www.photonics21.org).Quintero-Campos, P.; Juárez-Rodríguez, MJ.; Morais, S.; Maquieira Catala, Á. (2020). Multiparametric Highly Sensitive Chemiluminescence Immunoassayfor Quantification of b-Lactam-Specific Immunoglobulin E. Analytical Chemistry. 92(21):14608-14615. https://doi.org/10.1021/acs.analchem.0c03020S14608146159221World Health Organization. WHO Report on Surveillance of Antibiotic Consumption; 2018, 27–28.Doña, I., Torres, M. J., Montañez, M. I., & Fernández, T. D. (2017). In Vitro Diagnostic Testing for Antibiotic Allergy. Allergy, Asthma & Immunology Research, 9(4), 288. doi:10.4168/aair.2017.9.4.288Ariza, A., Garzon, D., Abánades, D. R., de los Ríos, V., Vistoli, G., Torres, M. J., … Pérez-Sala, D. (2012). Protein haptenation by amoxicillin: High resolution mass spectrometry analysis and identification of target proteins in serum. Journal of Proteomics, 77, 504-520. doi:10.1016/j.jprot.2012.09.030Romano, A., & Warrington, R. (2014). Antibiotic Allergy. Immunology and Allergy Clinics of North America, 34(3), 489-506. doi:10.1016/j.iac.2014.03.003Ansotegui, I. J., Melioli, G., Canonica, G. W., Caraballo, L., Villa, E., Ebisawa, M., … Zuberbier, T. (2020). IgE allergy diagnostics and other relevant tests in allergy, a World Allergy Organization position paper. World Allergy Organization Journal, 13(2), 100080. doi:10.1016/j.waojou.2019.100080Han, M., Shin, S., Park, H., Park, K. U., Park, M. H., & Song, E. Y. (2013). Comparison of Three Multiple Allergen Simultaneous Tests: RIDA Allergy Screen, MAST Optigen, and Polycheck Allergy. BioMed Research International, 2013, 1-6. doi:10.1155/2013/340513Park, D. J., Lee, J., Kim, S.-Y., Kwon, H. J., Lee, H. K., & Kim, Y. (2019). Evaluation of AdvanSure AlloScreen Max Panel With 92 Different Allergens for Detecting Allergen-Specific IgE. American Journal of Clinical Pathology, 151(6), 628-637. doi:10.1093/ajcp/aqz023Bojcukova, J., Vlas, T., Forstenlechner, P., & Panzner, P. (2019). Comparison of two multiplex arrays in the diagnostics of allergy. Clinical and Translational Allergy, 9(1). doi:10.1186/s13601-019-0270-yWurpts, G., Aberer, W., Dickel, H., Brehler, R., Jakob, T., Kreft, B., … Brockow, K. (2020). Guideline on diagnostic procedures for suspected hypersensitivity to beta-lactam antibiotics. Allergologie select, 4(01), 11-43. doi:10.5414/alx02104eHan, X., Cao, M., Wu, M., Wang, Y.-J., Yu, C., Zhang, C., … Huang, W. (2019). A paper-based chemiluminescence immunoassay device for rapid and high-throughput detection of allergen-specific IgE. The Analyst, 144(8), 2584-2593. doi:10.1039/c8an02020ePark, K. H., Lee, J., Sim, D. W., & Lee, S. C. (2018). Comparison of Singleplex Specific IgE Detection Immunoassays: ImmunoCAP Phadia 250 and Immulite 2000 3gAllergy. Annals of Laboratory Medicine, 38(1), 23-31. doi:10.3343/alm.2018.38.1.23Kosnik, M., Silar, M., Zidarn, M., & Korosec, P. (2017). Comparison of the diagnostic value of two assays for specific immunoglobulin E in suspected beta lactam allergy. Medicinski pregled, 70(7-8), 209-215. doi:10.2298/mpns1708209kBlumenthal, K. G., Peter, J. G., Trubiano, J. A., & Phillips, E. J. (2019). Antibiotic allergy. The Lancet, 393(10167), 183-198. doi:10.1016/s0140-6736(18)32218-9Wong, T., Atkinson, A., t’ Jong, G., Rieder, M. J., Chan, E. S., & Abrams, E. M. (2020). Beta-lactam allergy in the paediatric population. Paediatrics & Child Health, 25(1), 62-62. doi:10.1093/pch/pxz179Stone, C. A., Trubiano, J., Coleman, D. T., Rukasin, C. R. F., & Phillips, E. J. (2019). The challenge of de‐labeling penicillin allergy. Allergy, 75(2), 273-288. doi:10.1111/all.13848Zhang, S., Zhang, Z., Shi, W., Eremin, S. A., & Shen, J. (2006). Development of a Chemiluminescent ELISA for Determining Chloramphenicol in Chicken Muscle. Journal of Agricultural and Food Chemistry, 54(16), 5718-5722. doi:10.1021/jf060275jLiu, Y.-C., Jiang, W., Chen, Y.-J., Xiao, Y., Shi, J.-L., Qiao, Y.-B., … Wang, Q. (2013). A novel chemiluminescent ELISA for detecting furaltadone metabolite, 3-amino-5-morpholinomethyl-2-oxazolidone (AMOZ) in fish, egg, honey and shrimp samples. Journal of Immunological Methods, 395(1-2), 29-36. doi:10.1016/j.jim.2013.06.006Zhao, Z., Batley, M., D’Ambrosio, C., & Baldo, B. A. (2000). In vitro reactivity of penicilloyl and penicillanyl albumin and polylysine conjugates with IgE-antibody. Journal of Immunological Methods, 242(1-2), 43-51. doi:10.1016/s0022-1759(00)00213-1Medical, S.; Diagnostics, S. IMMULITE 3gAllergy Specific IgE Universal Kit. 2015, 1–35.Phadiatop, I. ImmunoCAP Determining the Presence of IgE Anti- Bodies with ImmunoCAP Phadiatop in Appropriate Evaluation of Atopic Patients. 2013, 1–2.Garvey, L. H., Ebo, D. G., Mertes, P., Dewachter, P., Garcez, T., Kopac, P., … Scherer, K. (2019). An EAACI position paper on the investigation of perioperative immediate hypersensitivity reactions. Allergy, 74(10), 1872-1884. doi:10.1111/all.1382

Determination of Allura Red and Tartrazine in Food Samples by Sequential Injection Analysis Combined with Voltammetric Detection at Antimony Film Electrode

An antimony film electrode prepared on-line and installed as part of a sequential injection system, was used as an electrochemical detector to determine azo dyes in food samples.The influence of several flow varia-bles were evaluated using a centra lcomposite design.In optimal conditions, the linear range of the calibration curve varied from 1–5 mM, with a limit of detection limit of 0.3 mM. The relative standard deviation of analytical repeatability was < 5.0 %.The method was validated by comparing the results obtained with those provided by HPLC; no significant difference were seen.Junta de Castilla y León Proyecto VA171U1

And QUIC meets IoT: performance assessment of MQTT over QUIC

We study the performance of the Message Queuing Telemetry Transport Protocol (MQTT) over QUIC. QUIC has been recently proposed as a new transport protocol, and it is gaining relevance at a very fast pace, favored by the support of key players, such as Google. It overcomes some of the limitations of the more widespread alternative, TCP, especially regarding the overhead of connection establishment. However, its use for Internet of Things (IoT) scenarios is still under consideration. In this paper we integrate a GO-based implementation of the QUIC protocol with MQTT, and we compare the performance of this combination with that exhibited by the more traditional MQTT/TLS/TCP approach. We use Linux Containers and we emulate various wireless network technologies by means of the ns3 simulator. The results of an extensive measurement campaign, show that QUIC protocol can indeed yield good performances for typical IoT use cases.The authors are grateful for the funding of the Industrial Doctorates Program from the University of Cantabria (Call 2018). This work has been partially supported by the Basque Government through the Elkartek program under the DIGITAL project (Grant agreement no. KK-2019/00095), as well as by the Spanish Government (Ministerio de Economía y Competitividad, Fondo Europeo de Desarrollo Regional, FEDER) by means of the project FIERCE: Future Internet Enabled Resilient smart CitiEs (RTI2018-093475-AI00)

Even lower latency in IIoT: evaluation of QUIC in industrial IoT scenarios

In this paper we analyze the performance of QUIC as a transport alternative for Internet of Things (IoT) services based on the Message Queuing Telemetry Protocol (MQTT). QUIC is a novel protocol promoted by Google, and was originally conceived to tackle the limitations of the traditional Transmission Control Protocol (TCP), specifically aiming at the reduction of the latency caused by connection establishment. QUIC use in IoT environments is not widespread, and it is therefore interesting to characterize its performance when in over such scenarios. We used an emulation-based platform, where we integrated QUIC and MQTT (using GO-based implementations) and compared their combined performance with the that exhibited by the traditional TCP/TLS approach. We used Linux containers as end devices, and the ns-3 simulator to emulate different network technologies, such as WiFi, cellular, and satellite, and varying conditions. The results evince that QUIC is indeed an appropriate protocol to guarantee robust, secure, and low latency communications over IoT scenarios.The authors are grateful for the funding of the Industrial Doctorates Program from the University of Cantabria (Call 2020). This work has been partially supported by the Basque Government through the Elkartek program under the DIGITAL project (grant agreement number KK-2019/00095), and by the Spanish Government (Ministerio de Economía y Competitividad, Fondo Europeo de Desarrollo Regional, FEDER) by means of the project FIERCE: Future Internet Enabled Resilient smart CitiEs (RTI2018-093475-AI00)

Assessment of Sensory Processing and Executive Functions at the School: Development, Reliability, and Validity of EPYFEI-Escolar

The authors thank the parents and teachers for their participation in this study. Especially to the teachers of the CEIP Ntra. Sra. Del Prado de Talavera de la Reina, Toledo (Spain) and CEIP Parque de las Infantas, Granada (Spain).The aim of this study was to determine the psychometric properties of the Assessment of Sensory Processing and Executive Functions at the School (EPYFEI-Escolar), a questionnaire designed to assess the sensory processing and executive functions as underlying processes for school participation. The total sample consisted of 536 children aged between 3 and 11 years old who lived in Spain. A total of 103 teachers completed the questionnaire. An exploratory factor analysis was conducted, which showed five main factors: (1) initiation, organization, execution, and supervision of the action; (2) inhibitory control; (3) sensory processing; (4) emotional self-regulation and play; and (5) self-competence. Some of these factors were similar to those found in the EPYFEI for parents in the home context. The reliability of the analysis was high, both for the whole questionnaire and for the factors it is composed of. The results provide evidence of the potential usefulness of the EPYFEI-Escolar in school contexts for determining academic needs and difficulties of children; moreover, this tool can also be used to plan intervention programs in the school environment according to the needs of each child and school.This study was supported by University of Málaga

Valoración Del Nivel De Ansiedad En La Persona Con Diabetes Durante La Pandemia

&nbsp;Introducción: En enfermería, la investigación permite tener un panorama sobre el sentir de las personas con diabetes, lo que daría como resultado mejoras en el cuidado. Objetivo: Determinar el nivel de ansiedad en el paciente diabético que asiste al Centro de Salud Oriente de San Juan del Río, en Querétaro, México. Metodología. Estudio transversal, descriptivo; muestreo por conveniencia. Se utilizó la escala de Síntomas de Ansiedad Asociados al COVID-19 que cuenta con 5 ítems; Alfa de Cronbach de .93; 5 opciones de respuesta en escala tipo Likert. El proyecto fue registrado y evaluado ante un Comité de Investigación y Bioética. En el análisis de datos se utilizó la estadística descriptiva. Resultados: Se encuestó a 46 pacientes, el 100% diabético. 76.1% era mujer. 53.2% tiene más de 10 años de padecer la enfermedad. 54.3% atiende su condición de salud con pastillas. 89.1% ha presentado alteraciones de la glucosa; 73.9% ha presentado cefalea e incremento de la tensión arterial el 41.3%. Al respecto de la actividad física, 63% ya no realiza lo que realizaba antes de la pandemia y vinculado a la alimentación, 60.9 incrementó el consumo. 28.2% de los participantes tuvieron un nivel de ansiedad moderado y 6.5% severo. Conclusiones: Se reconoce que quien padece diabetes presenta alteraciones no sólo a nivel biológico, donde además se suma una situación de confinamiento derivada de la pandemia. Es importante establecer modelos de atención que consideren los cambios epidemiológicos actuales. Introduction: In nursing, research allows an overview of the feelings of diabetic persons, which results in improvements in care. Objective: This paper focuses on determining the level of anxiety in diabetic patients at the Oriente Health Center of San Juan del Río in Querétaro, Mexico. &nbsp; Methodology:&nbsp; Descriptive cross-sectional study and convenience sampling technique was used. The Anxiety Symptoms associated to COVID-19 scale was applied on 5 items with 5 response options on a Likert-type scale. It had a Cronbach alpha of .93 and descriptive statistics were used in the data analysis. The project was registered and evaluated before a Research and Bioethics Committee. Results: 46 patients were surveyed and the results showed that 100% were diabetic. 76.1% were females while 53.2% have suffered for more than 10 years from the disease. 54.3% treat their health condition with pills, 89.1% have presented glucose alterations, 73.9% had a headache and an increase in blood pressure, 41.3% were involved in physical activity, 63% no longer do what they did before the pandemic which was linked to food, 60.9% experienced increased consumption, 28.2% of the participants had a moderate anxiety level, and 6.5% was severe. Conclusion: It is recognized that those who suffer from diabetes present alterations not only at a biological level, but a situation of confinement derived from the pandemic is also added. It is therefore important to establish models of care that consider current epidemiological change

Hábitos alimentarios y actividad física en relación con el sobrepeso y la obesidad en España

Introduction: The aim of this paper is to determine the eating habits and the physical activity in the Spanish population along 2012 and to analyze its relationship with overweight and obesity.Material and Methods: Cross-sectional study of the National Health Survey in 2012. A descriptive analysis of the eating habits and the physical activity was conducted within the general population and also within the genders for ages between 18 and 90 of age. Also, a multinomial logistic regression analysis was performed calculating the RRR adjusted to a series of sociodemographic variables. The goal was to assess the risk of overweight and obesity and know whether the individuals followed or not the recommendations given by the Spanish Society of Community Nutrition (SENC) in relation to eating habits, alcohol consumption and regular physical activity.Results: Less than 50% of the Spanish population followed the recommendations given by the SENC in the consumption of pasta and rice, vegetables, sausages and sweets. Women significantly followed the recommendations of taking fruits, vegetables, dairy products, fish, sausages, soft drinks, fast food and snacks and men followed the recommendations of pasta and rice, bread and cereals and legumes, in a statistically significant manner in both cases. More than a half of those surveyed (81.4%) did not physical activity during their free time being men the ones who performed physical activity more frequently. Daily consumption of fruit was associated with a higher risk of overweight (RRR adjusted=0.77; p=.008) and occasional consumption of sweets compared to the daily one showed a higher risk of overweight (RRR adjusted=0.83; p=.005) and obesity (RRR adjusted=0.73; p&lt;.001). Physical activity several times a week or monthly decreased the latter risk significantly (p&lt;.001).Conclusions: The Spanish population does not exercise enough in its free time. Association between eating habits and overweight and obesity is not clear and this paper follows the lead of the contradictions found in literature.Introducción: El objetivo del presente estudio es analizar los hábitos de alimentación y ejercicio físico de la población española en el año 2012 y su relación con el sobrepeso y la obesidad.Material y Métodos: Estudio transversal de la Encuesta Nacional de Salud de España del año 2012. Se realizó un análisis descriptivo de los hábitos alimentarios y actividad física en la población general y por sexos con edades comprendidas entre los 18 y los 90 años y un análisis de regresión logística multinomial calculando las RRR crudas y ajustadas a una serie de variables sociodemográficas para valorar el riesgo de presentar sobrepeso y obesidad según la adherencia a las recomendaciones nutricionales dadas por la Sociedad Española de Nutrición Comunitaria (SENC), el consumo o no de alcohol y la realización de actividad física regular.Resultados: Menos del 50% de la población española siguió las recomendaciones dadas por la SENC en el consumo de pasta y arroz, verduras, embutidos y dulces. Las mujeres siguieron más las recomendaciones de frutas, verduras, lácteos, pescado, embutidos, refrescos, comida rápida y aperitivos y los hombres las de pasta y arroz, pan y cereales y legumbres, en ambos casos con significación estadística. Más de la mitad de los encuestados (81,4%) no realizó actividad física durante su tiempo libre siendo los hombres los que la realizaban con mayor frecuencia. El consumo diario de fruta se asoció a un mayor riesgo de presentar sobrepeso (RRR ajustada=0,77; p=,008) y el consumo ocasional de dulces frente al diario se asoció a un mayor riesgo de tener sobrepeso (RRR ajustada=0,83; p=,005) y obesidad (RRR ajustada=0,73; p&lt;,001). La realización de actividad física varias veces en semana está directamente relacionada con la disminución significativa del exceso de peso (p&lt;,001).Conclusiones: La población española realiza poca actividad física en su tiempo libre. La asociación de determinados hábitos alimentarios y la presencia de exceso de peso no está clara, y este trabajo se suma a las contradicciones encontradas en la literatura al respecto

Subjects with allergic reactions to drugs show in vivo polarized patterns of cytokine expression depending on the chronology of the clinical reaction

Manuscrito aceptado el 28 junio de 2000Background: The mechanisms involved in adverse drug reactions with an immunologic basis (ADRIB) can be antibody dependent, mainly IgE or T cell dependent (sensitized T cells). These mechanisms are regulated by a number of cytokines, including IL-2, IL-4, IL-5, IFN-γ, and TNF-α, which follow the classical TH1/TH2 immunologic paradigm. Although evidence for this has been seen in ex vivo studies, the results are heterogeneous, and few in vivo studies have been carried out in subjects with ADRIB. Objective: We studied a group of patients who experienced either immediate reactions (n = 10) or nonimmediate reactions (n = 9) to drugs to determine the cytokine pattern profile during the acute stage of the response, as well as after recovery. Methods: PBMCs were taken at different time intervals of 24 hours or less and 7, 15, and 30 days after the onset of the reaction, and the specific cytokine transcription and production were determined by using quantitative competitive RT-PCR and ELISA, respectively. Results: There was a transient polarized pattern corresponding to a TH1 response with IL-2, IFN-γ, and TNF-α in nonimmediate reactions and to a TH2 response with IL-4 in immediate reactions. Conclusions: This is the first in vivo demonstration of these TH1/TH2 patterns in subjects with ADRIB and confirms that çan immunologic process is occurring related to the mechanisms involved in the pathologic manifestation. These findings are relevant to the understanding of the pathophysiologic mechanisms involved in ADRIB, suggesting that further studies in this direction are warranted.This work was supported in part by CICYT SAF 96/0240, Consejería de Salud de la Junta de Andalucía, FIS 98/0861, and the Spanish Society for Allergy and Clinical Immunology

Development, reliability, and validity of EPYFEI-Escolar

The aim of this study was to determine the psychometric properties of the Assessment of Sensory Processing and Executive Functions at the School (EPYFEI-Escolar), a questionnaire designed to assess the sensory processing and executive functions as underlying processes for school participation. The total sample consisted of 536 children aged between 3 and 11 years old who lived in Spain. A total of 103 teachers completed the questionnaire. An exploratory factor analysis was conducted, which showed five main factors: (1) initiation, organization, execution, and supervision of the action; (2) inhibitory control; (3) sensory processing; (4) emotional self-regulation and play; and (5) self-competence. Some of these factors were similar to those found in the EPYFEI for parents in the home context. The reliability of the analysis was high, both for the whole questionnaire and for the factors it is composed of. The results provide evidence of the potential usefulness of the EPYFEI-Escolar in school contexts for determining academic needs and difficulties of children; moreover, this tool can also be used to plan intervention programs in the school environment according to the needs of each child and school