Pragmatic aspects of spatial language acquisition and use across languages

Across languages, back is produced earlier and more frequently than front. This asymmetry has been attributed either to a conceptual/semantic asymmetry in the early meanings of these locatives (with back being more basic than front; conceptual immaturity account) or to the fact that Back configurations are inherently more ‘noteworthy’ than Front configurations (pragmatic account). Here, we tested the two accounts. In Study 1, children and adult speakers of English and Greek described Front/Back motion events. In Study 2, adult speakers of 10 additional languages described the same events. Despite cross-linguistic differences, speakers of all age and language groups typically used more Back than Front adpositions; furthermore, they often encoded Back information in occlusion verbs (e.g. hide) but no such verbs were available for Front. Thus, the front/back asymmetry is not due to children’s conceptual immaturity but should be linked to pragmatic factors that also shape adult spatial language production cross-linguistically

Intentionality and the Role of Labels in Categorization

Language has been shown to influence the ability to formcategories. Here we investigate whether linguistic labels areprivileged compared to other types of cues (e.g., numbers orsymbols), and whether labels exert their effects regardless ofwhether they are introduced intentionally. In a categorizationtask, we found that adults were more likely to use labels to de-termine category boundaries compared to numbers or symbols,and that these effects persisted in all intentionality manipula-tions. These findings suggest that labels have a powerful effecton categorization compared to other cues; most strikingly, la-bels (but not other cues) are used during categorization evenwhen people are specifically asked to ignore them. These re-sults provide novel support for the position that labels indicatecategory membership

SMCHD1 has separable roles in chromatin architecture and gene silencing that could be targeted in disease

Abstract The interplay between 3D chromatin architecture and gene silencing is incompletely understood. Here, we report a novel point mutation in the non-canonical SMC protein SMCHD1 that enhances its silencing capacity at endogenous developmental targets. Moreover, it also results in enhanced silencing at the facioscapulohumeral muscular dystrophy associated macrosatellite-array, D4Z4, resulting in enhanced repression of DUX4 encoded by this repeat. Heightened SMCHD1 silencing perturbs developmental Hox gene activation, causing a homeotic transformation in mice. Paradoxically, the mutant SMCHD1 appears to enhance insulation against other epigenetic regulators, including PRC2 and CTCF, while depleting long range chromatin interactions akin to what is observed in the absence of SMCHD1. These data suggest that SMCHD1’s role in long range chromatin interactions is not directly linked to gene silencing or insulating the chromatin, refining the model for how the different levels of SMCHD1-mediated chromatin regulation interact to bring about gene silencing in normal development and disease

Identification and functional characterisation of aquaporins in the grapevine, Vitis vinifera

© CSIRO 2009Plant aquaporins belong to a large superfamily of conserved proteins called the major intrinsic proteins (MIPs). There is limited information about the diversity of MIPs in grapevine, and their water transport capacity. The aim of the present study was to identify MIPs from grapevine and functionally characterise water transport of a subset of MIPs. Candidate genes were identified, by screening a Vitis vinifera L. (cv. Cabernet Sauvignon) cDNA library with gene specific probes, for aquaporin cDNAs encoding members of the plasma membrane intrinsic protein (PIP) and tonoplast intrinsic protein (TIP) subfamilies. The screen resulted in the identification of 11 full-length and two partial length aquaporin cDNAs. VvTIP2;1 isoforms had different 3′ UTRs, immediately upstream of the poly(A) tail, suggesting the presence of multiple cleavage sites for polyadenylation. Using published genome sequences of grapevine, we conducted a phylogenetic analysis of the MIPs with previously characterised MIPs from Arabidopsis. We identified 23 full-length MIP genes from the V. vinifera genome sequence of a near homozygous line (PN40024) that cluster into the four main subfamilies (and subgroups within) identified in other species. However, based on the identification of PIP2 genes in Cabernet Sauvignon that were not present in the PN40024 genome, there are likely to be more than 23 MIP genes in other heterozygous grapevine cultivars. Water transport capacity was determined for several PIPs and TIPs, by expression in Xenopus oocytes. Only VvPIP2 and VvTIP proteins function as water channels with the exception of VvPIP2;5. VvPIP2;5 differs from the water conducting VvPIP2;1 by the substitution of two highly conserved amino acids in Loop B (G97S, G100W), which was shown by homology modelling to likely form a hydrophobic block of the water pore.Megan C. Shelden, Susan M. Howitt, Brent N. Kaiser and Stephen D. Tyerma

SMCHD1 has separable roles in chromatin architecture and gene silencing that could be targeted in disease.

The interplay between 3D chromatin architecture and gene silencing is incompletely understood. Here, we report a novel point mutation in the non-canonical SMC protein SMCHD1 that enhances its silencing capacity at endogenous developmental targets. Moreover, it also results in enhanced silencing at the facioscapulohumeral muscular dystrophy associated macrosatellite-array, D4Z4, resulting in enhanced repression of DUX4 encoded by this repeat. Heightened SMCHD1 silencing perturbs developmental Hox gene activation, causing a homeotic transformation in mice. Paradoxically, the mutant SMCHD1 appears to enhance insulation against other epigenetic regulators, including PRC2 and CTCF, while depleting long range chromatin interactions akin to what is observed in the absence of SMCHD1. These data suggest that SMCHD1's role in long range chromatin interactions is not directly linked to gene silencing or insulating the chromatin, refining the model for how the different levels of SMCHD1-mediated chromatin regulation interact to bring about gene silencing in normal development and disease